LOCUS       BBa_I20250               928 bp    DNA     linear       22-Dec-2024
DEFINITION  Device used to characterize Ribosome Binding Sites through 
            measurement of RFP expression
ACCESSION   BBa_I20250
VERSION     BBa_I20250.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     CDS             61..735
                     /label=mrfp1
     misc_feature    61..741
                     /label=BBa_E1010
     misc_feature    742..766
                     /label=barcode
     misc_feature    775..854
                     /label=BBa_B0010
     stem_loop       786..829
                     /label=stem_loop
     misc_feature    863..903
                     /label=BBa_B0012
     stem_loop       870..889
                     /label=T7 TE
     polyA_site      890..903
                     /label=polya
     misc_feature    896..896
                     /label=stop
ORIGIN
        1 gaattgtccc tatcagtgat agagattgac atccctatca gtgatagaga tactgagcac
       61 gaattcgcgg ccgctctaga tggcttcctc cgaagacgtt atcaaagagt tcatgcgttt
      121 caaagttcgt atggaaggtt ccgttaacgg tcacgagttc gaaatcgaag gtgaaggtga
      181 aggtcgtccg tacgaaggta cccagaccgc taaactgaaa gttaccaaag gtggtccgct
      241 gccgttcgct tgggacatcc tgtccccgca gttccagtac ggttccaaag cttacgttaa
      301 acacccggct gacatcccgg actacctgaa actgtccttc ccggaaggtt tcaaatggga
      361 acgtgttatg aacttcgaag acggtggtgt tgttaccgtt acccaggact cctccctgca
      421 agacggtgag ttcatctaca aagttaaact gcgtggtacc aacttcccgt ccgacggtcc
      481 ggttatgcag aaaaaaacca tgggttggga agcttccacc gaacgtatgt acccggaaga
      541 cggtgctctg aaaggtgaaa tcaaaatgcg tctgaaactg aaagacggtg gtcactacga
      601 cgctgaagtt aaaaccacct acatggctaa aaaaccggtt cagctgccgg gtgcttacaa
      661 aaccgacatc aaactggaca tcacctccca caacgaagac tacaccatcg ttgaacagta
      721 cgaacgtgct gaaggtcgtc actccaccgg tgcttaataa cgctgatagt gctagtgtag
      781 atcgctacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
      841 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg
      901 ggtgggcctt tctgcgttta taatgcag
//