LOCUS       BBa_I20251               929 bp    DNA     linear       22-Nov-2024
DEFINITION  Device used to characterize Ribosome Binding Sites through 
            measurement of RFP expression
ACCESSION   BBa_I20251
VERSION     BBa_I20251.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     CDS             61..735
                     /label=mrfp1
     misc_feature    61..741
                     /label=BBa_E1010
     misc_feature    742..766
                     /label=barcode
     misc_feature    775..854
                     /label=BBa_B0010
     stem_loop       786..829
                     /label=stem_loop
     misc_feature    863..903
                     /label=BBa_B0012
     stem_loop       870..889
                     /label=T7 TE
     polyA_site      890..903
                     /label=polya
     misc_feature    896..896
                     /label=stop
ORIGIN
        1 gaattgtccc tatcagtgat agagattgac atccctatca gtgatagaga tactgagcac
       61 tactagtgcg gccgctctag atggcttcct ccgaagacgt tatcaaagag ttcatgcgtt
      121 tcaaagttcg tatggaaggt tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg
      181 aaggtcgtcc gtacgaaggt acccagaccg ctaaactgaa agttaccaaa ggtggtccgc
      241 tgccgttcgc ttgggacatc ctgtccccgc agttccagta cggttccaaa gcttacgtta
      301 aacacccggc tgacatcccg gactacctga aactgtcctt cccggaaggt ttcaaatggg
      361 aacgtgttat gaacttcgaa gacggtggtg ttgttaccgt tacccaggac tcctccctgc
      421 aagacggtga gttcatctac aaagttaaac tgcgtggtac caacttcccg tccgacggtc
      481 cggttatgca gaaaaaaacc atgggttggg aagcttccac cgaacgtatg tacccggaag
      541 acggtgctct gaaaggtgaa atcaaaatgc gtctgaaact gaaagacggt ggtcactacg
      601 acgctgaagt taaaaccacc tacatggcta aaaaaccggt tcagctgccg ggtgcttaca
      661 aaaccgacat caaactggac atcacctccc acaacgaaga ctacaccatc gttgaacagt
      721 acgaacgtgc tgaaggtcgt cactccaccg gtgcttaata acgctgatag tgctagtgta
      781 gatcgctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
      841 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
      901 gggtgggcct ttctgcgttt ataatgcag
//