BBa_I20251 1 BBa_I20251 Device used to characterize Ribosome Binding Sites through measurement of RFP expression 2007-08-14T11:00:00Z 2015-08-31T04:07:39Z . Device is based on I2056 and contains a partial biobrick site (Spe1, Not1, Xba1) between R0040 and RFP. To insert a BB promoter for testing, digest the promoter Xba1 and Spe1 and the kit EcoR1 and Xba1. Use .5ul of each restriction enzyme during the ligation to ensure the formation of two mixed sites. This design provides the proper spacing, identical to actual construction conditions. false false _11_ 0 2010 11 Not in stock false . false Jason Kelly, Karen Wong, Nishant Bhat annotation1941302 1 barcode range1941302 1 742 766 annotation1941300 1 mrfp1 range1941300 1 61 735 annotation1941304 1 stem_loop range1941304 1 786 829 annotation1941297 1 -35 range1941297 1 20 25 annotation1941307 1 polya range1941307 1 890 903 annotation1941301 1 BBa_E1010 range1941301 1 61 741 annotation1941308 1 stop range1941308 1 896 896 annotation1941298 1 TetR 2 range1941298 1 26 44 annotation1941295 1 TetR 1 range1941295 1 1 19 annotation1941303 1 BBa_B0010 range1941303 1 775 854 annotation1941296 1 BBa_R0040 range1941296 1 1 54 annotation1941299 1 -10 range1941299 1 43 48 annotation1941305 1 BBa_B0012 range1941305 1 863 903 annotation1941306 1 T7 TE range1941306 1 870 889 BBa_I20251_sequence 1 gaattgtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagtgcggccgctctagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttataatgcag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z