##gff-version 3
##sequence-region BBa_I20252 1 924
BBa_I20252	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I20252	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I20252	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I20252	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I20252	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I20252	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I20252	.	ribosome_entry_site	63	75	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_I20252	.	ribosome_entry_site	69	72	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_I20252	.	ribosome_entry_site	63	75	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_I20252	.	engineered_region	63	75	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_I20252	.	terminator	796	924	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_I20252	.	terminator	796	875	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I20252	.	engineered_region	796	875	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I20252	.	stem_loop	807	850	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I20252	.	terminator	884	924	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I20252	.	engineered_region	884	924	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I20252	.	stop_codon	917	917	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I20252	.	stem_loop	891	910	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I20252	.	polyA_site	911	924	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I20252	.	CDS	82	787	.	+	0	ID=BBa_E1010;Name=BBa_E1010
BBa_I20252	.	engineered_tag	763	787	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_I20252	.	CDS	82	756	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
>BBa_I20252
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacacaggaaagtact
agatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacga
gttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggt
ccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgaca
tcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgt
taccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtcc
gacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctga
aaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaa
aaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatc
gttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctac
tagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaac
gctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata