##gff-version 3
##sequence-region BBa_I20257 1 3912
BBa_I20257	.	engineered_region	1187	3912	.	+	0	ID=BBa_I20253;Name=BBa_I20253
BBa_I20257	.	CDS	1202	3856	.	+	0	ID=BBa_I2032;Name=BBa_I2032;Parent=BBa_I20253
BBa_I20257	.	promoter	3699	3726	.	+	0	ID=annotation1928674;Name=putative E. coli promoter;Parent=BBa_I2032
BBa_I20257	.	terminator	3865	3912	.	+	0	ID=BBa_B0016;Name=BBa_B0016;Parent=BBa_I20253
BBa_I20257	.	ribosome_entry_site	1187	1195	.	+	0	ID=BBa_B0073;Name=BBa_B0073;Parent=BBa_I20253
BBa_I20257	.	engineered_region	1	1178	.	+	0	ID=BBa_S03914;Name=BBa_S03914
BBa_I20257	.	promoter	1	44	.	+	0	ID=BBa_R0184;Name=BBa_R0184;Parent=BBa_S03914
BBa_I20257	.	sequence_feature	31	31	.	+	0	ID=annotation1932680;Name=Center of binding site symmetry;Parent=BBa_R0184
BBa_I20257	.	operator	20	44	.	+	0	ID=annotation1932679;Name=lacI binding site;Parent=BBa_R0184
BBa_I20257	.	promoter	1	20	.	+	0	ID=annotation1932678;Name=BBa_R0183 fragment;Parent=BBa_R0184
BBa_I20257	.	engineered_region	53	1178	.	+	0	ID=BBa_S03910;Name=BBa_S03910;Parent=BBa_S03914
BBa_I20257	.	CDS	68	1178	.	+	0	ID=BBa_C0013;Name=BBa_C0013;Parent=BBa_S03910
BBa_I20257	.	engineered_tag	1154	1178	.	+	0	ID=annotation2214005;Name=Help:Barcodes;Parent=BBa_C0013
BBa_I20257	.	ribosome_entry_site	53	61	.	+	0	ID=BBa_B0073;Name=BBa_B0073;Parent=BBa_S03910
>BBa_I20257
tcatacgactcactataggggaattgtgagcggataacaattcctactagagtcacaccactactagatgaaaccagta
acgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgttt
ctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggc
gggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgatt
aaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcgg
cggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgt
ggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcc
catgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccat
taagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcgga
acgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcg
atgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggata
tctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcg
cctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgccc
gtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaa
tgcagctggcacgacaggtttcccgactggaaagcgggcagtaataacactgatagtgctagtgtagatcactactaga
gtcacaccactactagatgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttc
aacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtg
aagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcat
cactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccg
acagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaa
ccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcg
tatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaag
aaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcata
aggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgcca
aaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgca
ggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtg
gctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgt
ttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtc
gccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaac
cggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggc
tcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttc
ccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggac
tgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcggg
tgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtct
ccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtac
agcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgc
gatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgtt
gctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgaga
acactggtgaaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatggctggcttacggtgttactcg
cagtgtgactaagcgttcagtcatgacgctggcttacgggtccaaagagttcggcttccgtcaacaagtgctggaagat
accattcagccagctattgattccggcaagggtctgatgttcactcagccgaatcaggctgctggatacatggctaagc
tgatttgggaatctgtgagcgtgacggtggtagctgcggttgaagcaatgaactggcttaagtctgctgctaagctgct
ggctgctgaggtcaaagataagaagactggagagattcttcgcaagcgttgcgctgtgcattgggtaactcctgatggt
ttccctgtgtggcaggaatacaagaagcctattcagacgcgcttgaacctgatgttcctcggtcagttccgcttacagc
ctaccattaacaccaacaaagatagcgagattgatgcacacaaacaggagtctggtatcgctcctaactttgtacacag
ccaagacggtagccaccttcgtaagactgtagtgtgggcacacgagaagtacggaatcgaatcttttgcactgattcac
gactccttcggtaccattccggctgacgctgcgaacctgttcaaagcagtgcgcgaaactatggttgacacatatgagt
cttgtgatgtactggctgatttctacgaccagttcgctgaccagttgcacgagtctcaattggacaaaatgccagcact
tccggctaaaggtaacttgaacctccgtgacatcttagagtcggacttcgcgttcgcgtaataatactagagctagcat
aaccccttggggcctctaaacgggtcttgaggggttttttg