Autoregulated synthesis of T7 RNAP with lacI negative feedback (orthogonal translation)
An early version of a virtual machine. T7 RNAP synthesis is autoregulated. Basal amount of E. coli RNAP are needed to "boot up" the system. A lacI negative feedback loop is included to maintain a stable level of T7 RNAP.
This protein generator uses an orthogonal RBS that is not recognized by E. coli. Use I2041 for expression of T7 RNAP in E. coli. The downstream terminator is a T7 terminator. This protein generator is designed for use in a virtual machine encoding orthogonal ribosomes and RNAP.
This sequence is based on the T7lac promoter described by Dubendorff and Studier (JMB 1991, 219, 45-59). The only modification is the mutation at -16.
A mutant version of the T7lac promoter designed by Dubendorff and Studier. The promoter is based on the strong ??10 T7 promoter but incorporates an A->C mutation at -16. The LacI operator site is a partially symmetric 25bp sequence.
The -16 mutation was included to weaken the consensus promoter as described by Imburgio and co-workers (see BBa_R0183 for more information)
Bacteriophage T7
Gene 1 coding region from bacteriophage T7 encoding a RNAP polymerase (RNAP). This single-protein RNAP recognizes a 21 bp consensus promoter sequence and is orthogonal to E. coli RNAP.
T7 gene 1 specific transcriptional terminator
LacI repressor protein, without SsrA degradation tag. No Barcode.
Conversion of the iGEM parts registry to SBOL2.1