LOCUS       BBa_I20264               917 bp    DNA     linear       22-Nov-2024
DEFINITION  RBS Measkit testing B0033
ACCESSION   BBa_I20264
VERSION     BBa_I20264.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     RBS             44..54
                     /label=RBS-4\Weaker
     RBS             50..53
                     /label=RBS
     misc_feature    44..54
                     /label=BBa_B0033
     RBS             44..54
                     /label=BBa_B0033
     CDS             61..780
                     /label=GFP protein
     CDS             61..780
                     /label=BBa_E0040
     stem_loop       800..843
                     /label=stem_loop
     misc_feature    789..868
                     /label=BBa_B0010
     terminator      789..868
                     /label=BBa_B0010
     stem_loop       884..903
                     /label=T7 TE
     misc_feature    910..910
                     /label=stop
     polyA_site      904..917
                     /label=polya
     misc_feature    877..917
                     /label=BBa_B0012
     terminator      877..917
                     /label=BBa_B0012
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagtcacaca ggactactag
       61 atgcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
      121 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga
      181 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt
      241 gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag
      301 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc
      361 aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt
      421 aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa
      481 ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga
      541 atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac
      601 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
      661 ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt
      721 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaataataa
      781 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
      841 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
      901 gcctttctgc gtttata
//