LOCUS       BBa_I20271              2778 bp    DNA     linear       16-Jun-2024
DEFINITION  T7 autogene
ACCESSION   BBa_I20271
VERSION     BBa_I20271.1  
FEATURES             Location/Qualifiers
     promoter        1..20
                     /label=BBa_R0183 fragment
     misc_feature    20..44
                     /label=lacI binding site
     misc_feature    31..31
                     /label=Center of binding site symmetry
     promoter        1..44
                     /label=BBa_R0184
     RBS             53..61
                     /label=BBa_B0073
     promoter        2565..2592
                     /label=putative E. coli promoter
     CDS             68..2722
                     /label=BBa_I2032
     terminator      2731..2778
                     /label=BBa_B0016
ORIGIN
        1 tcatacgact cactataggg gaattgtgag cggataacaa ttcctactag agtcacacca
       61 ctactagatg aacacgatta acatcgctaa gaacgacttc tctgacatcg aactggctgc
      121 tatcccgttc aacactctgg ctgaccatta cggtgagcgt ttagctcgcg aacagttggc
      181 ccttgagcat gagtcttacg agatgggtga agcacgcttc cgcaagatgt ttgagcgtca
      241 acttaaagct ggtgaggttg cggataacgc tgccgccaag cctctcatca ctaccctact
      301 ccctaagatg attgcacgca tcaacgactg gtttgaggaa gtgaaagcta agcgcggcaa
      361 gcgcccgaca gccttccagt tcctgcaaga aatcaagccg gaagccgtag cgtacatcac
      421 cattaagacc actctggctt gcctaaccag tgctgacaat acaaccgttc aggctgtagc
      481 aagcgcaatc ggtcgggcca ttgaggacga ggctcgcttc ggtcgtatcc gtgaccttga
      541 agctaagcac ttcaagaaaa acgttgagga acaactcaac aagcgcgtag ggcacgtcta
      601 caagaaagca tttatgcaag ttgtcgaggc tgacatgctc tctaagggtc tactcggtgg
      661 cgaggcgtgg tcttcgtggc ataaggaaga ctctattcat gtaggagtac gctgcatcga
      721 gatgctcatt gagtcaaccg gaatggttag cttacaccgc caaaatgctg gcgtagtagg
      781 tcaagactct gagactatcg aactcgcacc tgaatacgct gaggctatcg caacccgtgc
      841 aggtgcgctg gctggcatct ctccgatgtt ccaaccttgc gtagttcctc ctaagccgtg
      901 gactggcatt actggtggtg gctattgggc taacggtcgt cgtcctctgg cgctggtgcg
      961 tactcacagt aagaaagcac tgatgcgcta cgaagacgtt tacatgcctg aggtgtacaa
     1021 agcgattaac attgcgcaaa acaccgcatg gaaaatcaac aagaaagtcc tagcggtcgc
     1081 caacgtaatc accaagtgga agcattgtcc ggtcgaggac atccctgcga ttgagcgtga
     1141 agaactcccg atgaaaccgg aagacatcga catgaatcct gaggctctca ccgcgtggaa
     1201 acgtgctgcc gctgctgtgt accgcaagga caaggctcgc aagtctcgcc gtatcagcct
     1261 tgagttcatg cttgagcaag ccaataagtt tgctaaccat aaggccatct ggttccctta
     1321 caacatggac tggcgcggtc gtgtttacgc tgtgtcaatg ttcaacccgc aaggtaacga
     1381 tatgaccaaa ggactgctta cgctggcgaa aggtaaacca atcggtaagg aaggttacta
     1441 ctggctgaaa atccacggtg caaactgtgc gggtgtcgat aaggttccgt tccctgagcg
     1501 catcaagttc attgaggaaa accacgagaa catcatggct tgcgctaagt ctccactgga
     1561 gaacacttgg tgggctgagc aagattctcc gttctgcttc cttgcgttct gctttgagta
     1621 cgctggggta cagcaccacg gcctgagcta taactgctcc cttccgctgg cgtttgacgg
     1681 gtcttgctct ggcatccagc acttctccgc gatgctccga gatgaggtag gtggtcgcgc
     1741 ggttaacttg cttcctagtg aaaccgttca ggacatctac gggattgttg ctaagaaagt
     1801 caacgagatt ctacaagcag acgcaatcaa tgggaccgat aacgaagtag ttaccgtgac
     1861 cgatgagaac actggtgaaa tctctgagaa agtcaagctg ggcactaagg cactggctgg
     1921 tcaatggctg gcttacggtg ttactcgcag tgtgactaag cgttcagtca tgacgctggc
     1981 ttacgggtcc aaagagttcg gcttccgtca acaagtgctg gaagatacca ttcagccagc
     2041 tattgattcc ggcaagggtc tgatgttcac tcagccgaat caggctgctg gatacatggc
     2101 taagctgatt tgggaatctg tgagcgtgac ggtggtagct gcggttgaag caatgaactg
     2161 gcttaagtct gctgctaagc tgctggctgc tgaggtcaaa gataagaaga ctggagagat
     2221 tcttcgcaag cgttgcgctg tgcattgggt aactcctgat ggtttccctg tgtggcagga
     2281 atacaagaag cctattcaga cgcgcttgaa cctgatgttc ctcggtcagt tccgcttaca
     2341 gcctaccatt aacaccaaca aagatagcga gattgatgca cacaaacagg agtctggtat
     2401 cgctcctaac tttgtacaca gccaagacgg tagccacctt cgtaagactg tagtgtgggc
     2461 acacgagaag tacggaatcg aatcttttgc actgattcac gactccttcg gtaccattcc
     2521 ggctgacgct gcgaacctgt tcaaagcagt gcgcgaaact atggttgaca catatgagtc
     2581 ttgtgatgta ctggctgatt tctacgacca gttcgctgac cagttgcacg agtctcaatt
     2641 ggacaaaatg ccagcacttc cggctaaagg taacttgaac ctccgtgaca tcttagagtc
     2701 ggacttcgcg ttcgcgtaat aatactagag ctagcataac cccttggggc ctctaaacgg
     2761 gtcttgaggg gttttttg
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