BBa_I20278
type: ComponentDefinition
persistent identity: BBa I20278
display id: BBa_I20278
version: 1
was generated by: iGEM to SBOL conversion
description: T7 autogene
created: 2008-04-07T11:00:00Z
modified: 2015-08-31T04:07:40Z
mutable provenance: na
owned by: james
owned by: myers
mutable description: T7 RNAP transcribes itself and a LacI gene represses the same promoter. An orthogonal RBS control the expression of both genes.
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: I20278 is a redesign of I20257. The objective of the redesign was to increase the expression levels of LacI.
star: false
creator: Bartholomew Canton
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_I20275
component: BBa_I20271
sequence annotation: BBa_I20271
sequence annotation: BBa_I20275
sequence: https://synbiohub.org/public/igem/BBa_I20278_sequence/1

BBa_I20275
type: Component
persistent identity: component2266582
display id: component2266582
version: 1
top level: BBa_I20278
definition: BBa_I20275
access: public

BBa_I20271
type: Component
persistent identity: component2266576
display id: component2266576
version: 1
top level: BBa_I20278
definition: BBa_I20271
access: public

BBa_I20271
type: SequenceAnnotation
persistent identity: annotation2266576
display id: annotation2266576
version: 1
top level: BBa_I20278
location: https://synbiohub.org/public/igem/BBa_I20278/annotation2266576/range2266576/1
component: BBa_I20271

https://synbiohub.org/public/igem/BBa_I20278/annotation2266576/range2266576/1
type: Range
persistent identity: range2266576
display id: range2266576
version: 1
top level: BBa_I20278
direction: direction/reverse
start: 1
end: 2778
orientation: inline

BBa_I20275
type: SequenceAnnotation
persistent identity: annotation2266582
display id: annotation2266582
version: 1
top level: BBa_I20278
location: https://synbiohub.org/public/igem/BBa_I20278/annotation2266582/range2266582/1
component: BBa_I20275

https://synbiohub.org/public/igem/BBa_I20278/annotation2266582/range2266582/1
type: Range
persistent identity: range2266582
display id: range2266582
version: 1
top level: BBa_I20278
direction: direction/reverse
start: 2787
end: 3943
orientation: inline

BBa_I20271
type: ComponentDefinition
persistent identity: BBa I20271
display id: BBa_I20271
version: 1
was generated by: iGEM to SBOL conversion
description: T7 autogene
created: 2008-03-30T11:00:00Z
modified: 2015-08-31T04:07:39Z
mutable provenance: na
owned by: james
owned by: myers
mutable description: Expression of T7 RNAP is controlled by a Lac-repressible T7 promoter and an orthogonal ribosome binding site. T??, a terminator from bacteriophage T7 should end transcription with an ~80% efficiency.
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: na
star: false
creator: Bartholomew Canton
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0016
component: BBa_I2032
component: BBa_B0073
component: BBa_R0184
sequence annotation: BBa_R0184
sequence annotation: BBa_B0073
sequence annotation: BBa_I2032
sequence annotation: BBa_B0016
sequence: https://synbiohub.org/public/igem/BBa_I20271_sequence/1

BBa_B0016
type: Component
persistent identity: component1962109
display id: component1962109
version: 1
top level: BBa_I20271
definition: BBa_B0016
access: public

BBa_I2032
type: Component
persistent identity: component1962108
display id: component1962108
version: 1
top level: BBa_I20271
definition: BBa_I2032
access: public

BBa_B0073
type: Component
persistent identity: component1962106
display id: component1962106
version: 1
top level: BBa_I20271
definition: BBa_B0073
access: public

BBa_R0184
type: Component
persistent identity: component1962105
display id: component1962105
version: 1
top level: BBa_I20271
definition: BBa_R0184
access: public

BBa_R0184
type: SequenceAnnotation
persistent identity: annotation1962105
display id: annotation1962105
version: 1
top level: BBa_I20271
location: https://synbiohub.org/public/igem/BBa_I20271/annotation1962105/range1962105/1
component: BBa_R0184

https://synbiohub.org/public/igem/BBa_I20271/annotation1962105/range1962105/1
type: Range
persistent identity: range1962105
display id: range1962105
version: 1
top level: BBa_I20271
direction: direction/reverse
start: 1
end: 44
orientation: inline

BBa_B0073
type: SequenceAnnotation
persistent identity: annotation1962106
display id: annotation1962106
version: 1
top level: BBa_I20271
location: https://synbiohub.org/public/igem/BBa_I20271/annotation1962106/range1962106/1
component: BBa_B0073

https://synbiohub.org/public/igem/BBa_I20271/annotation1962106/range1962106/1
type: Range
persistent identity: range1962106
display id: range1962106
version: 1
top level: BBa_I20271
direction: direction/reverse
start: 53
end: 61
orientation: inline

BBa_I2032
type: SequenceAnnotation
persistent identity: annotation1962108
display id: annotation1962108
version: 1
top level: BBa_I20271
location: https://synbiohub.org/public/igem/BBa_I20271/annotation1962108/range1962108/1
component: BBa_I2032

https://synbiohub.org/public/igem/BBa_I20271/annotation1962108/range1962108/1
type: Range
persistent identity: range1962108
display id: range1962108
version: 1
top level: BBa_I20271
direction: direction/reverse
start: 68
end: 2722
orientation: inline

BBa_B0016
type: SequenceAnnotation
persistent identity: annotation1962109
display id: annotation1962109
version: 1
top level: BBa_I20271
location: https://synbiohub.org/public/igem/BBa_I20271/annotation1962109/range1962109/1
component: BBa_B0016

https://synbiohub.org/public/igem/BBa_I20271/annotation1962109/range1962109/1
type: Range
persistent identity: range1962109
display id: range1962109
version: 1
top level: BBa_I20271
direction: direction/reverse
start: 2731
end: 2778
orientation: inline

BBa_I20275
type: ComponentDefinition
persistent identity: BBa I20275
display id: BBa_I20275
version: 1
was generated by: iGEM to SBOL conversion
description: LacI protein generator
created: 2008-04-07T11:00:00Z
modified: 2015-08-31T04:07:40Z
mutable provenance: na
owned by: james
owned by: myers
mutable description: A mutant T7 promoter and an orthogonal RBS control expression of LacI
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: na
star: false
creator: Bartholomew Canton
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_S03910
component: BBa_R0180
sequence annotation: BBa_R0180
sequence annotation: BBa_S03910
sequence: https://synbiohub.org/public/igem/BBa_I20275_sequence/1

BBa_S03910
type: Component
persistent identity: component2245860
display id: component2245860
version: 1
top level: BBa_I20275
definition: BBa_S03910
access: public

BBa_R0180
type: Component
persistent identity: component2245856
display id: component2245856
version: 1
top level: BBa_I20275
definition: BBa_R0180
access: public

BBa_R0180
type: SequenceAnnotation
persistent identity: annotation2245856
display id: annotation2245856
version: 1
top level: BBa_I20275
location: https://synbiohub.org/public/igem/BBa_I20275/annotation2245856/range2245856/1
component: BBa_R0180

https://synbiohub.org/public/igem/BBa_I20275/annotation2245856/range2245856/1
type: Range
persistent identity: range2245856
display id: range2245856
version: 1
top level: BBa_I20275
direction: direction/reverse
start: 1
end: 23
orientation: inline

BBa_S03910
type: SequenceAnnotation
persistent identity: annotation2245860
display id: annotation2245860
version: 1
top level: BBa_I20275
location: https://synbiohub.org/public/igem/BBa_I20275/annotation2245860/range2245860/1
component: BBa_S03910

https://synbiohub.org/public/igem/BBa_I20275/annotation2245860/range2245860/1
type: Range
persistent identity: range2245860
display id: range2245860
version: 1
top level: BBa_I20275
direction: direction/reverse
start: 32
end: 1157
orientation: inline

BBa_B0073
type: ComponentDefinition
persistent identity: BBa B0073
display id: BBa_B0073
version: 1
was generated by: iGEM to SBOL conversion
description: Specialized RBS
created: 2006-05-01T11:00:00Z
modified: 2015-08-31T04:07:21Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Bartholomew Canton
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0073_sequence/1

BBa_R0184
type: ComponentDefinition
persistent identity: BBa R0184
display id: BBa_R0184
version: 1
was generated by: iGEM to SBOL conversion
description: T7 promoter (lacI repressible)
created: 2007-04-25T11:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: This sequence is based on the T7lac promoter described by Dubendorff and Studier (JMB 1991, 219, 45-59). The only modification is the mutation at -16.
owned by: james
owned by: myers
mutable description: A mutant version of the T7lac promoter designed by Dubendorff and Studier. The promoter is based on the strong ??10 T7 promoter but incorporates an A->C mutation at -16. The LacI operator site is a partially symmetric 25bp sequence.
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The -16 mutation was included to weaken the consensus promoter as described by Imburgio and co-workers (see BBa_R0183 for more information)
star: false
creator: Bartholomew Canton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0183 fragment
sequence annotation: lacI binding site
sequence annotation: Center of binding site symmetry
sequence: https://synbiohub.org/public/igem/BBa_R0184_sequence/1

BBa_R0183 fragment
type: SequenceAnnotation
persistent identity: annotation1932678
display id: annotation1932678
version: 1
top level: BBa_R0184
location: https://synbiohub.org/public/igem/BBa_R0184/annotation1932678/range1932678/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0184/annotation1932678/range1932678/1
type: Range
persistent identity: range1932678
display id: range1932678
version: 1
top level: BBa_R0184
direction: direction/forward
start: 1
end: 20
orientation: inline

lacI binding site
type: SequenceAnnotation
persistent identity: annotation1932679
display id: annotation1932679
version: 1
top level: BBa_R0184
location: https://synbiohub.org/public/igem/BBa_R0184/annotation1932679/range1932679/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0184/annotation1932679/range1932679/1
type: Range
persistent identity: range1932679
display id: range1932679
version: 1
top level: BBa_R0184
direction: direction/forward
start: 20
end: 44
orientation: inline

Center of binding site symmetry
type: SequenceAnnotation
persistent identity: annotation1932680
display id: annotation1932680
version: 1
top level: BBa_R0184
location: https://synbiohub.org/public/igem/BBa_R0184/annotation1932680/range1932680/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_R0184/annotation1932680/range1932680/1
type: Range
persistent identity: range1932680
display id: range1932680
version: 1
top level: BBa_R0184
direction: direction/forward
start: 31
end: 31
orientation: inline

BBa_S03910
type: ComponentDefinition
persistent identity: BBa S03910
display id: BBa_S03910
version: 1
was generated by: iGEM to SBOL conversion
description: B0073:C0013
created: 2007-11-29T12:00:00Z
modified: 2015-05-08T01:14:32Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _84_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Bartholomew Canton
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_C0013
component: BBa_B0073
sequence annotation: BBa_B0073
sequence annotation: BBa_C0013
sequence: https://synbiohub.org/public/igem/BBa_S03910_sequence/1

BBa_C0013
type: Component
persistent identity: component2243962
display id: component2243962
version: 1
top level: BBa_S03910
definition: lacI
access: public

BBa_B0073
type: Component
persistent identity: component2243960
display id: component2243960
version: 1
top level: BBa_S03910
definition: BBa_B0073
access: public

BBa_B0073
type: SequenceAnnotation
persistent identity: annotation2243960
display id: annotation2243960
version: 1
top level: BBa_S03910
location: https://synbiohub.org/public/igem/BBa_S03910/annotation2243960/range2243960/1
component: BBa_B0073

https://synbiohub.org/public/igem/BBa_S03910/annotation2243960/range2243960/1
type: Range
persistent identity: range2243960
display id: range2243960
version: 1
top level: BBa_S03910
direction: direction/reverse
start: 1
end: 9
orientation: inline

BBa_C0013
type: SequenceAnnotation
persistent identity: annotation2243962
display id: annotation2243962
version: 1
top level: BBa_S03910
location: https://synbiohub.org/public/igem/BBa_S03910/annotation2243962/range2243962/1
component: BBa_C0013

https://synbiohub.org/public/igem/BBa_S03910/annotation2243962/range2243962/1
type: Range
persistent identity: range2243962
display id: range2243962
version: 1
top level: BBa_S03910
direction: direction/reverse
start: 16
end: 1126
orientation: inline

BBa_I2032
type: ComponentDefinition
persistent identity: BBa I2032
display id: BBa_I2032
version: 1
was generated by: iGEM to SBOL conversion
description: T7 RNA polymerase
created: 2007-04-11T11:00:00Z
modified: 2016-10-14T06:52:19Z
mutable provenance: Bacteriophage T7
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Gene 1 coding region from bacteriophage T7 encoding a RNAP polymerase (RNAP). This single-protein RNAP recognizes a 21 bp consensus promoter sequence and is orthogonal to E. coli RNAP.
discontinued: false
dominant: true
experience: experience/None
group u list: _11_
m user id: 27399
owner id: 135
owning group id: 84
sample status: In stock
status: status/Available
bookmark: false
mutable notes: n/a
star: false
creator: Bartholomew Canton
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: putative E. coli promoter
sequence: https://synbiohub.org/public/igem/BBa_I2032_sequence/1

putative E. coli promoter
type: SequenceAnnotation
persistent identity: annotation1928674
display id: annotation1928674
version: 1
top level: BBa_I2032
location: https://synbiohub.org/public/igem/BBa_I2032/annotation1928674/range1928674/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I2032/annotation1928674/range1928674/1
type: Range
persistent identity: range1928674
display id: range1928674
version: 1
top level: BBa_I2032
direction: direction/forward
start: 2498
end: 2525
orientation: inline

BBa_B0016
type: ComponentDefinition
persistent identity: BBa B0016
display id: BBa_B0016
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (T7 RNAP specific, T_Phi)
created: 2003-12-04T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Sri Kosuri
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: T7 gene 1 specific transcriptional terminator
discontinued: false
dominant: true
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Sri Kosuri
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_B0016_sequence/1

BBa_R0180
type: ComponentDefinition
persistent identity: BBa R0180
display id: BBa_R0180
version: 1
was generated by: iGEM to SBOL conversion
description: T7 RNAP promoter
created: 2005-09-25T11:00:00Z
modified: 2015-05-08T01:14:15Z
owned by: james
owned by: myers
mutable description: T7 promoter with an A->T mutation at the -16 position of the consensus sequence
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Barry Canton
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_R0180_sequence/1

lacI
type: ComponentDefinition
persistent identity: BBa C0013
display id: BBa_C0013
version: 1
was generated by: iGEM to SBOL conversion
description: Repressor, lacI
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: www.ncbi.nlm.nih.gov
owned by: james
owned by: myers
mutable description: LacI repressor protein, without SsrA degradation tag. No Barcode.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Yong-Su Jin (Fighting Darwins)
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0013_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214005
display id: annotation2214005
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0013/annotation2214005/range2214005/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0013/annotation2214005/range2214005/1
type: Range
persistent identity: range2214005
display id: range2214005
version: 1
top level: lacI
direction: direction/forward
start: 1087
end: 1111
orientation: inline

https://synbiohub.org/public/igem/BBa_I20278_sequence/1
type: Sequence
persistent identity: BBa I20278 sequence
display id: BBa_I20278_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcatacgactcactataggggaattgtgagcggataacaattcctactagagtcacaccactactagatgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctggcttacgggtccaaagagttcg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I20271_sequence/1
type: Sequence
persistent identity: BBa I20271 sequence
display id: BBa_I20271_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcatacgactcactataggggaattgtgagcggataacaattcctactagagtcacaccactactagatgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctggcttacgggtccaaagagttcg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I20275_sequence/1
type: Sequence
persistent identity: BBa I20275 sequence
display id: BBa_I20275_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: ttatacgactcactatagggagatactagagtcacaccactactagatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0073_sequence/1
type: Sequence
persistent identity: BBa B0073 sequence
display id: BBa_B0073_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacaccac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0184_sequence/1
type: Sequence
persistent identity: BBa R0184 sequence
display id: BBa_R0184_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcatacgactcactataggggaattgtgagcggataacaattcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S03910_sequence/1
type: Sequence
persistent identity: BBa S03910 sequence
display id: BBa_S03910_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacaccactactagatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I2032_sequence/1
type: Sequence
persistent identity: BBa I2032 sequence
display id: BBa_I2032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctggcttacgggtccaaagagttcggcttccgtcaacaagtgctggaagataccattcagccagctattgattccggcaagggtctgatgtt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0016_sequence/1
type: Sequence
persistent identity: BBa B0016 sequence
display id: BBa_B0016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctagcataaccccttggggcctctaaacgggtcttgaggggttttttg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0180_sequence/1
type: Sequence
persistent identity: BBa R0180 sequence
display id: BBa_R0180_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatacgactcactatagggaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0013_sequence/1
type: Sequence
persistent identity: BBa C0013 sequence
display id: BBa_C0013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataacactgatagtgctagtgtagatcac
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z