BBa_I2028
type: ComponentDefinition
persistent identity: BBa I2028
display id: BBa_I2028
version: 1
was generated by: iGEM to SBOL conversion
description: RhlR Receiver Test (R1075.B0034.C0071.B0015.R0071.E0022)
created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:40Z
owned by: james
owned by: myers
mutable description: rhlR receiver test: -to test basal activity, give no input of chemical signal, and reporter indicates TIPs leakage -to test binding, put in solution of chemical signal made by rhlI (AI-2), the inducer binds to the lasR protein to activate the promoter R0071, to make TIPs that will express reporter
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Sara Neves (Fighting Darwins)
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_C0071
component: BBa_B0034
component: BBa_R1075
component: BBa_E0022
component: BBa_R0071
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_R1075
sequence annotation: BBa_B0034
sequence annotation: BBa_C0071
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0071
sequence annotation: BBa_E0022
sequence: https://synbiohub.org/public/igem/BBa_I2028_sequence/1

BBa_C0071
type: Component
persistent identity: component959136
display id: component959136
version: 1
top level: BBa_I2028
definition: rhlR
access: public

BBa_B0034
type: Component
persistent identity: component959126
display id: component959126
version: 1
top level: BBa_I2028
definition: BBa_B0034
access: public

BBa_R1075
type: Component
persistent identity: component959121
display id: component959121
version: 1
top level: BBa_I2028
definition: const.
access: public

BBa_E0022
type: Component
persistent identity: component959180
display id: component959180
version: 1
top level: BBa_I2028
definition: ECFP
access: public

BBa_R0071
type: Component
persistent identity: component959168
display id: component959168
version: 1
top level: BBa_I2028
definition: RhlR+C4
access: public

BBa_B0012
type: Component
persistent identity: component959152
display id: component959152
version: 1
top level: BBa_I2028
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component959142
display id: component959142
version: 1
top level: BBa_I2028
definition: BBa_B0010
access: public

BBa_R1075
type: SequenceAnnotation
persistent identity: annotation959121
display id: annotation959121
version: 1
top level: BBa_I2028
location: https://synbiohub.org/public/igem/BBa_I2028/annotation959121/range959121/1
component: BBa_R1075

https://synbiohub.org/public/igem/BBa_I2028/annotation959121/range959121/1
type: Range
persistent identity: range959121
display id: range959121
version: 1
top level: BBa_I2028
direction: direction/forward
start: 1
end: 49
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation959126
display id: annotation959126
version: 1
top level: BBa_I2028
location: https://synbiohub.org/public/igem/BBa_I2028/annotation959126/range959126/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I2028/annotation959126/range959126/1
type: Range
persistent identity: range959126
display id: range959126
version: 1
top level: BBa_I2028
direction: direction/forward
start: 58
end: 69
orientation: inline

BBa_C0071
type: SequenceAnnotation
persistent identity: annotation959136
display id: annotation959136
version: 1
top level: BBa_I2028
location: https://synbiohub.org/public/igem/BBa_I2028/annotation959136/range959136/1
component: BBa_C0071

https://synbiohub.org/public/igem/BBa_I2028/annotation959136/range959136/1
type: Range
persistent identity: range959136
display id: range959136
version: 1
top level: BBa_I2028
direction: direction/forward
start: 76
end: 837
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation959142
display id: annotation959142
version: 1
top level: BBa_I2028
location: https://synbiohub.org/public/igem/BBa_I2028/annotation959142/range959142/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I2028/annotation959142/range959142/1
type: Range
persistent identity: range959142
display id: range959142
version: 1
top level: BBa_I2028
direction: direction/forward
start: 871
end: 950
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation959152
display id: annotation959152
version: 1
top level: BBa_I2028
location: https://synbiohub.org/public/igem/BBa_I2028/annotation959152/range959152/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I2028/annotation959152/range959152/1
type: Range
persistent identity: range959152
display id: range959152
version: 1
top level: BBa_I2028
direction: direction/forward
start: 959
end: 999
orientation: inline

BBa_R0071
type: SequenceAnnotation
persistent identity: annotation959168
display id: annotation959168
version: 1
top level: BBa_I2028
location: https://synbiohub.org/public/igem/BBa_I2028/annotation959168/range959168/1
component: BBa_R0071

https://synbiohub.org/public/igem/BBa_I2028/annotation959168/range959168/1
type: Range
persistent identity: range959168
display id: range959168
version: 1
top level: BBa_I2028
direction: direction/forward
start: 1008
end: 1060
orientation: inline

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation959180
display id: annotation959180
version: 1
top level: BBa_I2028
location: https://synbiohub.org/public/igem/BBa_I2028/annotation959180/range959180/1
component: BBa_E0022

https://synbiohub.org/public/igem/BBa_I2028/annotation959180/range959180/1
type: Range
persistent identity: range959180
display id: range959180
version: 1
top level: BBa_I2028
direction: direction/forward
start: 1067
end: 1828
orientation: inline

const.
type: ComponentDefinition
persistent identity: BBa R1075
display id: BBa_R1075
version: 1
was generated by: iGEM to SBOL conversion
description: Constitutive Promoter II
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: Gaal, Gourse, et al.
owned by: james
owned by: myers
mutable description: constitutive promoter which outputs .4 TIPs point mutants of wild type rrnB promoter P1
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Chris Walsh (Fighting Darwins)
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R1075_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation309024
display id: annotation309024
version: 1
top level: const.
location: https://synbiohub.org/public/igem/BBa_R1075/annotation309024/range309024/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R1075/annotation309024/range309024/1
type: Range
persistent identity: range309024
display id: range309024
version: 1
top level: const.
direction: direction/forward
start: 15
end: 20
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation309020
display id: annotation309020
version: 1
top level: const.
location: https://synbiohub.org/public/igem/BBa_R1075/annotation309020/range309020/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R1075/annotation309020/range309020/1
type: Range
persistent identity: range309020
display id: range309020
version: 1
top level: const.
direction: direction/forward
start: 35
end: 40
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

RhlR+C4
type: ComponentDefinition
persistent identity: BBa R0071
display id: BBa_R0071
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (RhlR & C4-HSL regulated)
created: 2004-01-24T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: Pseudomonas aeruginosa rhlAB promoter
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Promoter activated by RhlR in concert with C4-HSL. C4-HSL is produced by RhlI, and acts as a quorum-sensing autoinducer. This is the natural sequence taken from Pseudomonas aeruginosa. Crosstalk: this promoter is also activated at a low level by LasR with its associated HSL.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The -10 and -35 sites are labeled as identified in [Pearson97]. The part begins with the RhlR binding site, and (rather arbitrarily) ends with the first transcribed base (+1).
star: true
creator: Ronny Krashinsky
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: RhlR
sequence annotation: -35
sequence annotation: -10
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0071_sequence/1

RhlR
type: SequenceAnnotation
persistent identity: annotation297102
display id: annotation297102
version: 1
top level: RhlR+C4
location: https://synbiohub.org/public/igem/BBa_R0071/annotation297102/range297102/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0071/annotation297102/range297102/1
type: Range
persistent identity: range297102
display id: range297102
version: 1
top level: RhlR+C4
direction: direction/forward
start: 1
end: 20
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation297103
display id: annotation297103
version: 1
top level: RhlR+C4
location: https://synbiohub.org/public/igem/BBa_R0071/annotation297103/range297103/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0071/annotation297103/range297103/1
type: Range
persistent identity: range297103
display id: range297103
version: 1
top level: RhlR+C4
direction: direction/forward
start: 19
end: 24
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation297104
display id: annotation297104
version: 1
top level: RhlR+C4
location: https://synbiohub.org/public/igem/BBa_R0071/annotation297104/range297104/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0071/annotation297104/range297104/1
type: Range
persistent identity: range297104
display id: range297104
version: 1
top level: RhlR+C4
direction: direction/forward
start: 42
end: 47
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation297105
display id: annotation297105
version: 1
top level: RhlR+C4
location: https://synbiohub.org/public/igem/BBa_R0071/annotation297105/range297105/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0071/annotation297105/range297105/1
type: Range
persistent identity: range297105
display id: range297105
version: 1
top level: RhlR+C4
direction: direction/forward
start: 53
end: 53
orientation: inline

ECFP
type: ComponentDefinition
persistent identity: BBa E0022
display id: BBa_E0022
version: 1
was generated by: iGEM to SBOL conversion
description: enhanced cyan fluorescent protein derived from A. victoria GFP
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:25Z
mutable provenance: Modified from <bb_part>BBa_E0021</bb_part>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Cyan fluorescent protein (ECFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_E0022 cyan fluorescent protein is based on BioBrick part BBa_E0021. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: BBa_E0022
sequence annotation: CFP (LVA)
sequence annotation: A
sequence annotation: SsrA
sequence annotation: 2
sequence: https://synbiohub.org/public/igem/BBa_E0022_sequence/1

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation7040
display id: annotation7040
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation7040/range7040/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_E0022/annotation7040/range7040/1
type: Range
persistent identity: range7040
display id: range7040
version: 1
top level: ECFP
direction: direction/forward
start: 1
end: 762
orientation: inline

CFP (LVA)
type: SequenceAnnotation
persistent identity: annotation2150
display id: annotation2150
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2150/range2150/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E0022/annotation2150/range2150/1
type: Range
persistent identity: range2150
display id: range2150
version: 1
top level: ECFP
direction: direction/forward
start: 1
end: 762
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation2155
display id: annotation2155
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2155/range2155/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_E0022/annotation2155/range2155/1
type: Range
persistent identity: range2155
display id: range2155
version: 1
top level: ECFP
direction: direction/forward
start: 69
end: 69
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation2153
display id: annotation2153
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2153/range2153/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_E0022/annotation2153/range2153/1
type: Range
persistent identity: range2153
display id: range2153
version: 1
top level: ECFP
direction: direction/forward
start: 719
end: 756
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation2154
display id: annotation2154
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2154/range2154/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_E0022/annotation2154/range2154/1
type: Range
persistent identity: range2154
display id: range2154
version: 1
top level: ECFP
direction: direction/forward
start: 757
end: 762
orientation: inline

rhlR
type: ComponentDefinition
persistent identity: BBa C0071
display id: BBa_C0071
version: 1
was generated by: iGEM to SBOL conversion
description: rhlR repressor/activator from P. aeruginosa PA3477 (+LVA)
created: 2004-01-26T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: P. aeruginosa PA3477
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcriptional regulator, in complex with N-butyryl-HSL, RhlR binds to the Rhl promoter
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: (1/28/04 Edit Jamboree) (0) No BB sites (1) ATG (2) TAATAA (3) Blast checks out (4) No codon changes (5) Maybe chassis compatability (check by experiment) (6) Codon usage not optimal (but looks OK) (7) ssrA tag added
star: true
creator: Debra Lin, Srini Devadas, David Gray, Ronny Krashinsky, and Chris Zheng Liu, Boopers
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: rhlR
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0071_sequence/1

rhlR
type: SequenceAnnotation
persistent identity: annotation301192
display id: annotation301192
version: 1
top level: rhlR
location: https://synbiohub.org/public/igem/BBa_C0071/annotation301192/range301192/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0071/annotation301192/range301192/1
type: Range
persistent identity: range301192
display id: range301192
version: 1
top level: rhlR
direction: direction/forward
start: 1
end: 723
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation306523
display id: annotation306523
version: 1
top level: rhlR
location: https://synbiohub.org/public/igem/BBa_C0071/annotation306523/range306523/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0071/annotation306523/range306523/1
type: Range
persistent identity: range306523
display id: range306523
version: 1
top level: rhlR
direction: direction/forward
start: 724
end: 756
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213995
display id: annotation2213995
version: 1
top level: rhlR
location: https://synbiohub.org/public/igem/BBa_C0071/annotation2213995/range2213995/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0071/annotation2213995/range2213995/1
type: Range
persistent identity: range2213995
display id: range2213995
version: 1
top level: rhlR
direction: direction/forward
start: 763
end: 787
orientation: inline

https://synbiohub.org/public/igem/BBa_I2028_sequence/1
type: Sequence
persistent identity: BBa I2028 sequence
display id: BBa_I2028_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttaaatttcctcttttcaggccggaataactccctataatgcgccaccatactagagaaagaggagaaatactagatgaggaatgacggaggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgtcctggaaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcccttcacccggccgaagaccgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggccgtggatccggcgatcctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccggatgctctggaacgaggctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttccgtggcgcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgctgacccagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagatcctgcaatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttccaccacaagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctcatcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcctgtgaaatctggcagttaccgttagctttcgaattggctaaaaagtgttctactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R1075_sequence/1
type: Sequence
persistent identity: BBa R1075 sequence
display id: BBa_R1075_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttaaatttcctcttttcaggccggaataactccctataatgcgccacca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0071_sequence/1
type: Sequence
persistent identity: BBa R0071 sequence
display id: BBa_R0071_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcctgtgaaatctggcagttaccgttagctttcgaattggctaaaaagtgttc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0022_sequence/1
type: Sequence
persistent identity: BBa E0022 sequence
display id: BBa_E0022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0071_sequence/1
type: Sequence
persistent identity: BBa C0071 sequence
display id: BBa_C0071_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaggaatgacggaggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgtcctggaaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcccttcacccggccgaagaccgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggccgtggatccggcgatcctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccggatgctctggaacgaggctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttccgtggcgcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgctgacccagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagatcctgcaatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttccaccacaagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctcatcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z