LOCUS BBa_I20280 3999 bp DNA linear 25-Nov-2024 DEFINITION T7 autogenerator ACCESSION BBa_I20280 VERSION BBa_I20280.1 FEATURES Location/Qualifiers promoter 1..20 /label=BBa_R0183 fragment misc_feature 20..44 /label=lacI binding site misc_feature 31..31 /label=Center of binding site symmetry promoter 1..44 /label=BBa_R0184 RBS 53..61 /label=BBa_B0073 promoter 2565..2592 /label=putative E. coli promoter CDS 68..2722 /label=BBa_I2032 terminator 2731..2778 /label=BBa_B0016 misc_feature 1..2778 /label=BBa_I20271 promoter 2787..2809 /label=BBa_R0182 RBS 2818..2826 /label=BBa_B0073 misc_feature 3919..3943 /label=Help:Barcodes CDS 2833..3943 /label=BBa_C0013 misc_feature 2818..3943 /label=BBa_S03910 misc_feature 2787..3943 /label=BBa_I20272 misc_feature 1..3943 /label=BBa_I20276 terminator 3952..3999 /label=BBa_B0016 ORIGIN 1 tcatacgact cactataggg gaattgtgag cggataacaa ttcctactag agtcacacca 61 ctactagatg aacacgatta acatcgctaa gaacgacttc tctgacatcg aactggctgc 121 tatcccgttc aacactctgg ctgaccatta cggtgagcgt ttagctcgcg aacagttggc 181 ccttgagcat gagtcttacg agatgggtga agcacgcttc cgcaagatgt ttgagcgtca 241 acttaaagct ggtgaggttg cggataacgc tgccgccaag cctctcatca ctaccctact 301 ccctaagatg attgcacgca tcaacgactg gtttgaggaa gtgaaagcta agcgcggcaa 361 gcgcccgaca gccttccagt tcctgcaaga aatcaagccg gaagccgtag cgtacatcac 421 cattaagacc actctggctt gcctaaccag tgctgacaat acaaccgttc aggctgtagc 481 aagcgcaatc ggtcgggcca ttgaggacga ggctcgcttc ggtcgtatcc gtgaccttga 541 agctaagcac ttcaagaaaa acgttgagga acaactcaac aagcgcgtag ggcacgtcta 601 caagaaagca tttatgcaag ttgtcgaggc tgacatgctc tctaagggtc tactcggtgg 661 cgaggcgtgg tcttcgtggc ataaggaaga ctctattcat gtaggagtac gctgcatcga 721 gatgctcatt gagtcaaccg gaatggttag cttacaccgc caaaatgctg gcgtagtagg 781 tcaagactct gagactatcg aactcgcacc tgaatacgct gaggctatcg caacccgtgc 841 aggtgcgctg gctggcatct ctccgatgtt ccaaccttgc gtagttcctc ctaagccgtg 901 gactggcatt actggtggtg gctattgggc taacggtcgt cgtcctctgg cgctggtgcg 961 tactcacagt aagaaagcac tgatgcgcta cgaagacgtt tacatgcctg aggtgtacaa 1021 agcgattaac attgcgcaaa acaccgcatg gaaaatcaac aagaaagtcc tagcggtcgc 1081 caacgtaatc accaagtgga agcattgtcc ggtcgaggac atccctgcga ttgagcgtga 1141 agaactcccg atgaaaccgg aagacatcga catgaatcct gaggctctca ccgcgtggaa 1201 acgtgctgcc gctgctgtgt accgcaagga caaggctcgc aagtctcgcc gtatcagcct 1261 tgagttcatg cttgagcaag ccaataagtt tgctaaccat aaggccatct ggttccctta 1321 caacatggac tggcgcggtc gtgtttacgc tgtgtcaatg ttcaacccgc aaggtaacga 1381 tatgaccaaa ggactgctta cgctggcgaa aggtaaacca atcggtaagg aaggttacta 1441 ctggctgaaa atccacggtg caaactgtgc gggtgtcgat aaggttccgt tccctgagcg 1501 catcaagttc attgaggaaa accacgagaa catcatggct tgcgctaagt ctccactgga 1561 gaacacttgg tgggctgagc aagattctcc gttctgcttc cttgcgttct gctttgagta 1621 cgctggggta cagcaccacg gcctgagcta taactgctcc cttccgctgg cgtttgacgg 1681 gtcttgctct ggcatccagc acttctccgc gatgctccga gatgaggtag gtggtcgcgc 1741 ggttaacttg cttcctagtg aaaccgttca ggacatctac gggattgttg ctaagaaagt 1801 caacgagatt ctacaagcag acgcaatcaa tgggaccgat aacgaagtag ttaccgtgac 1861 cgatgagaac actggtgaaa tctctgagaa agtcaagctg ggcactaagg cactggctgg 1921 tcaatggctg gcttacggtg ttactcgcag tgtgactaag cgttcagtca tgacgctggc 1981 ttacgggtcc aaagagttcg gcttccgtca acaagtgctg gaagatacca ttcagccagc 2041 tattgattcc ggcaagggtc tgatgttcac tcagccgaat caggctgctg gatacatggc 2101 taagctgatt tgggaatctg tgagcgtgac ggtggtagct gcggttgaag caatgaactg 2161 gcttaagtct gctgctaagc tgctggctgc tgaggtcaaa gataagaaga ctggagagat 2221 tcttcgcaag cgttgcgctg tgcattgggt aactcctgat ggtttccctg tgtggcagga 2281 atacaagaag cctattcaga cgcgcttgaa cctgatgttc ctcggtcagt tccgcttaca 2341 gcctaccatt aacaccaaca aagatagcga gattgatgca cacaaacagg agtctggtat 2401 cgctcctaac tttgtacaca gccaagacgg tagccacctt cgtaagactg tagtgtgggc 2461 acacgagaag tacggaatcg aatcttttgc actgattcac gactccttcg gtaccattcc 2521 ggctgacgct gcgaacctgt tcaaagcagt gcgcgaaact atggttgaca catatgagtc 2581 ttgtgatgta ctggctgatt tctacgacca gttcgctgac cagttgcacg agtctcaatt 2641 ggacaaaatg ccagcacttc cggctaaagg taacttgaac ctccgtgaca tcttagagtc 2701 ggacttcgcg ttcgcgtaat aatactagag ctagcataac cccttggggc ctctaaacgg 2761 gtcttgaggg gttttttgta ctagagtaat acgtctcact atagggagat actagagtca 2821 caccactact agatgaaacc agtaacgtta tacgatgtcg cagagtatgc cggtgtctct 2881 tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa 2941 aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc acaacaactg 3001 gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct gcacgcgccg 3061 tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag cgtggtggtg 3121 tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg 3181 caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc cattgctgtg 3241 gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca gacacccatc 3301 aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca tctggtcgca 3361 ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg 3421 cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat agcggaacgg 3481 gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct gaatgagggc 3541 atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc 3601 attaccgagt ccgggctgcg cgttggtgcg gatatctcgg tagtgggata cgacgatacc 3661 gaagacagct catgttatat cccgccgtta accaccatca aacaggattt tcgcctgctg 3721 gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt gaagggcaat 3781 cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc 3841 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg 3901 gaaagcgggc agtaataaca ctgatagtgc tagtgtagat cactactaga gctagcataa 3961 ccccttgggg cctctaaacg ggtcttgagg ggttttttg //