T7 gene 1 specific transcriptional terminator
T7 RNAP transcribes itself and a LacI gene represses the same promoter. An orthogonal RBS control the expression of both genes.
I20278 is a redesign of I20257. The objective of the redesign was to increase the expression levels of LacI.
Expression of T7 RNAP is controlled by a Lac-repressible T7 promoter and an orthogonal ribosome binding site. T??, a terminator from bacteriophage T7 should end transcription with an ~80% efficiency.
A mutant T7 promoter and an orthogonal RBS control expression of LacI
This sequence is based on the T7lac promoter described by Dubendorff and Studier (JMB 1991, 219, 45-59). The only modification is the mutation at -16.
A mutant version of the T7lac promoter designed by Dubendorff and Studier. The promoter is based on the strong ??10 T7 promoter but incorporates an A->C mutation at -16. The LacI operator site is a partially symmetric 25bp sequence.
The -16 mutation was included to weaken the consensus promoter as described by Imburgio and co-workers (see BBa_R0183 for more information)
Bacteriophage T7
Gene 1 coding region from bacteriophage T7 encoding a RNAP polymerase (RNAP). This single-protein RNAP recognizes a 21 bp consensus promoter sequence and is orthogonal to E. coli RNAP.
T7 promoter with an A->T mutation at the -16 position of the consensus sequence
LacI repressor protein, without SsrA degradation tag. No Barcode.
Conversion of the iGEM parts registry to SBOL2.1