LOCUS BBa_I20282 6221 bp DNA linear 22-Dec-2024 DEFINITION Protein Balloon Generator ACCESSION BBa_I20282 VERSION BBa_I20282.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 RBS 21..32 /label=b0034 CDS 39..305 /label=gvpB CDS 381..647 /label=gvpR CDS 676..1603 /label=gvpN CDS 1617..2384 /label=gvpF CDS 2384..2652 /label=gvpG CDS 2653..3862 /label=gvpL CDS 3880..4167 /label=gvpS CDS 4121..4405 /label=gvpK CDS 4427..4729 /label=gvpJ CDS 4766..5644 /label=gvpT CDS 5668..6084 /label=gvpU misc_feature 2865..2865 /label=gvpL g213a misc_feature 2970..2970 /label=gvpL g318a misc_feature 3003..3003 /label=gvpL g351a misc_feature 3348..3348 /label=gvpL g696a misc_feature 3364..3763 /label=10 repeats of 40bp induced durring mutagenesis misc_feature 738..738 /label=c->a misc_feature 2723..2723 /label=t->c misc_feature 2792..2792 /label=t->c misc_feature 3242..3242 /label=t->a misc_feature 4552..4552 /label=t->a misc_feature 5090..5090 /label=g->a misc_feature 5153..5153 /label=g->a misc_feature 3324..3363 /label=First copy of 40bp section misc_feature 21..6084 /label=BBa_I750016 stem_loop 6104..6147 /label=stem_loop misc_feature 6093..6172 /label=BBa_B0010 terminator 6093..6172 /label=BBa_B0010 stem_loop 6188..6207 /label=T7 TE misc_feature 6214..6214 /label=stop polyA_site 6208..6221 /label=polya misc_feature 6181..6221 /label=BBa_B0012 terminator 6181..6221 /label=BBa_B0012 ORIGIN 1 aaagaggaga aatactagag aaagaggaga aatactagat gtctattcaa aaaagtacta 61 atagttcaag tttagcagaa gtcattgacc gtattttaga taaaggaatt gttattgatg 121 cttttgcaag agtttctgtt gtaggaattg aaattttaac gattgaagcg cgagtggtta 181 ttgccagtgt tgatacatgg ttacgctatg cagaagcagt agggcttctt cgtgacgacg 241 tagaagaaaa cggtcttcct gaacgttcaa attcaagtga agggcagccg cgttttagta 301 tttaatatga agtggtatgg aaccttcctt ttttgagggt ggttccagct tttagatcta 361 actattggag gctactaaaa atggaaatta aaaaaattat gcaagccgtg aacgactttt 421 tcggtgaaca cgtagctcct cctcataaaa ttacctcggt ggaagctact gaagatgaag 481 gttggagagt tattgttgaa gtcattgaag aacgagaata tatgaaaaaa tacgccaaag 541 atgaaatgct cggaacgtac gagtgctttg taaataaaga aaaagaagtc atttcattca 601 aacgactcga cgtcagatat agaagcgcca ttggcattga agcataaacg ataagatggc 661 aggaggaacg taaaaatgac cgtcttaaca gacaaaagga aaaaaggcag tggagctttt 721 atacaagatg acgagacaaa agaggttctt tcaagagcgc tgagctattt aaaatccggc 781 tattccattc attttacagg tcctgccggc ggaggcaaaa cctctttagc gcgagcgctt 841 gctaaaaaga gaaagcgtcc tgtaatgctg atgcacggga atcacgagct caacaacaaa 901 gatttaattg gcgattttac gggatacacg agcaaaaaag taatcgacca gtacgttcgt 961 tctgtctata aaaaagatga acaggtgagt gaaaactggc aggatggccg attgcttgaa 1021 gctgtaaaaa atggctatac gctgatttac gacgaattta ctcgttctaa gcctgcgacg 1081 aataatatct ttctatcgat attagaagaa ggcgtgctgc cgctgtatgg agtaaaaatg 1141 accgatcctt ttgtgcgcgt gcatcccgat ttccgcgtca tcttcacaag caatccagct 1201 gagtatgccg gcgtatatga tacgcaagat gcgcttctcg acaggttaat taccatgttt 1261 attgattata aagacatcga cagagagaca gcgattttaa cggagaaaac ggacgtagaa 1321 gaagatgaag cgcgcacaat tgtaacgctc gtagcaaacg tgcgaaaccg ctctggagac 1381 gaaaacagca gcggacttag cctgcgggct tcgcttatga tcgctaccct tgccacgcag 1441 caagacattc ctatcgatgg aagtgacgaa gattttcaaa cgttatgtat cgatattttg 1501 catcatccgc ttaccaaatg tttggatgaa gaaaatgcaa aaagcaaagc cgaaaaaatc 1561 attttagaag aatgtaagaa tatagacact gaagaaaagt aaaggagctt gaaaacatga 1621 gtgaaacaaa cgaaacaggt atttatattt ttagcgccat tcaaacggat aaagacgaag 1681 aatttggcgc cgtggaagta gaaggaacaa aagctgaaac atttttgatt cgctacaaag 1741 acgcggctat ggtagcagct gaagtaccga tgaaaattta tcatcctaat cgccaaaatt 1801 tattaatgca tcaaaacgca gtagcagcga ttatggacaa gaacgatacg gttattccaa 1861 tcagctttgg gaatgtattc aaatcaaaag aagacgtaaa agttcttttg gaaaaccttt 1921 atccgcagtt tgaaaagctg tttccagcga tcaaaggaaa aattgaagtc ggtttaaaag 1981 taattgggaa aaaagaatgg cttgagaaaa aagtaaacga aaatcctgaa cttgagaaag 2041 tatcagcatc cgtaaaagga aaatcagaag cagccggtta ttatgagcgt attcaacttg 2101 gaggaatggc tcaaaagatg tttacttccc tgcaaaaaga agtcaagaca gatgtgtttt 2161 ctccgcttga agaagcagcg gaagcagcaa aagcaaatga gccaacgggc gaaacgatgc 2221 ttttaaacgc gtctttctta attaaccgag aagatgaagc gaagtttgat gaaaaagtaa 2281 atgaagcgca tgaaaactgg aaagacaaag ccgattttca ttacagcggt ccttggcctg 2341 cttataattt tgtgaacatt cgcctaaaag tagaagagaa ataacgtgct tcacaaatta 2401 gtaaccgcac ccattaacct tgtagtgaaa atcggcgaaa aagtacagga agaagctgat 2461 aaacagctat atgaccttcc gacgattcag caaaagctca ttcagcttca aatgatgttt 2521 gagcttggtg aaattccaga agaagcgttt caagaaaaag aagatgaatt gttaatgagg 2581 tacgaaattg cgaaacgcag agaaattgaa caatgggaag agctaacaca aaaaagaaat 2641 gaggaatcct agatgggaga attactgtat ttatacggtt taattccaac aaaagaagca 2701 gcagccatag agccgtttcc atcttataag gggtttgacg gagaacattc actgtaccca 2761 attgcgtttg atcaggtgac ggctgtagtt tctaagctgg atgctgacac ctattcagaa 2821 aaagtgattc aagaaaaaat ggagcaggat atgagctggc tgcaagaaaa agcatttcat 2881 catcacgaaa cggtagccgc tttgtacgaa gaatttacga tcattccatt aaaattttgc 2941 accatttata aaggtgaaga aagtctgcaa gcagctattg agattaacaa agaaaagata 3001 gaaaattcac tgacgctgct tcaaggaaat gaagagtgga atgtgaaaat ttactgtgat 3061 gatacagagc ttaaaaaagg aatcagcgaa acgaatgaaa gcgtgaaagc gaaaaaacaa 3121 gaaattagtc acttatcacc aggaagacag ttttttgaaa agaaaaaaat agatcagctg 3181 attgaaaaag aattagagct tcacaaaaac aaagtgtgtg aagagataca tgacaagcta 3241 aaagaattat cgctttatga ctctgttaaa aagaattgga gcaaagacgt aactggcgca 3301 gctgaacaga tggcgtggaa cagcgtgttt cttctcccgt ctctgcaaat tactaagttc 3361 gtacgtgttt cttctcccgt ctctgcaaat tactaagttc gtacgtgttt cttctcccgt 3421 ctctgcaaat tactaagttc gtacgtgttt cttctcccgt ctctgcaaat tactaagttc 3481 gtacgtgttt cttctcccgt ctctgcaaat tactaagttc gtacgtgttt cttctcccgt 3541 ctctgcaaat tactaagttc gtacgtgttt cttctcccgt ctctgcaaat tactaagttc 3601 gtacgtgttt cttctcccgt ctctgcaaat tactaagttc gtacgtgttt cttctcccgt 3661 ctctgcaaat tactaagttc gtacgtgttt cttctcccgt ctctgcaaat tactaagttc 3721 gtacgtgttt cttctcccgt ctctgcaaat tactaagttc gtaaacgaaa tagaagagct 3781 tcagcaaagg cttgaaaata aaggctggaa gtttgaagtg acgggaccat ggccgcccta 3841 tcatttctcg agctttgcgt aaagtgagga attaacatta tgtctcttaa acaatccatg 3901 gagaataaag atattgctct tattgatatt ttagatgtca ttttagataa aggagtcgcc 3961 attaaaggag acttaatcat ttccatagct ggcgtcgatt tagtgtattt ggatttgcgg 4021 gtgcttattt cttcggttga aacgcttgtg caagcaaaag aaggaaatca caaaccaatc 4081 acttctgaac aatttgataa acaaaaggag gaattaatgg atgcaaccgg tcagccaagc 4141 aaatggacga atccacttgg atcctgatca agctgaacaa ggcttagcgc agcttgtgat 4201 gacagttatt gagctattga ggcaaatcgt tgaacgtcat gccatgaggc gggtggaggg 4261 tggaacgttg acggacgaac aaattgaaaa cttaggaatt gcactaatga acttagaaga 4321 aaaaatggac gagttgaaag aggtgttcgg tctggatgca gaagatttaa atattgatct 4381 tggaccgcta ggcagcctgc tttaagcggt cagtaggagg aacagtatgg cagtcgaaca 4441 taatatgcag tcaagtacga ttgtagatgt gctcgaaaag attttggata aaggagtcgt 4501 tatagcgggg gacatcaccg taggaattgc agatgtcgag ctattaacga taaagatccg 4561 cttgattgtg gcttcggttg ataaggcaaa agaaatcggc atggactggt gggaaaatga 4621 tccgtatctc agttcaaaag gagccaataa caaagcgctc gaagaagaaa ataaaatgct 4681 gcatgagcgg ttaaaaacgc ttgaagaaaa aatagaaacg aaacgttaaa aactgtacgc 4741 tacttaaaaa atggagggat ttacaatggc aactgaaaca aaattagata acacacaggc 4801 agaaaacaag gaaaataaaa atgcggaaaa cggttcaaaa gaaaagaacg gttcaaaagc 4861 aagcaaaaca acaagcagcg ggccaatcaa acgagcggta gcaggaggca tcatcggtgc 4921 aacgattgga tatgtatcga ctcctgaaaa tcgaaaaagt ctccttgacc gcattgatac 4981 agacgaatta aaaagcaaag catctgattt aggaacaaag gtaaaagaaa aatcaaaaag 5041 cagcgtggcc agcctgaaaa catctgcggg aagcttgttt aaaaaagata aagataaatc 5101 aaaagatgat gaagaaaacg taaattcttc tagtagcgaa acagaagacg ataacgttca 5161 agagtacgac gagttaaaag aagaaaatca aactcttcaa gatcgcttat cacagcttga 5221 agaaaaaatg aacatgcttg ttgagcttag cctcaataaa aatcaagacg aagaagcgga 5281 agatacagat tccgacgaag aagagaacga tgagaacgat gaaaacgatg aaaacgagca 5341 ggacgatgaa aacgaagaag aaacatctaa gccacgtaaa aaggataaaa aagaagctga 5401 ggaagaagaa agtgaaagtg acgaagacag cgaggaagaa gaggaagatt ctcgctcaaa 5461 caaaaaaaat aaaaaagtaa aaacagaaga agaagacgaa gatgaaagcg aagaagaaaa 5521 aaaggaagcg aaaccaaaaa agtcaacagc taaaaaatca aaaaatacaa aagcaaagaa 5581 aaacacggac gaagaagatg atgaagcaac atctctttct agtgaagacg atacaacagc 5641 ctaagacgta aaggaggaaa gaaagacatg agtacaggcc cttctttttc aactaaagac 5701 aatacgcttg aatactttgt gaaagcttct aataaacacg gcttttcact tgatatttca 5761 ttaaatgtaa acggcgctgt gatttccggt accatgattt cagcaaaaga atactttgat 5821 tacttaagcg aaacgtttga agaaggcagt gaagtggctc aggcgctaag cgaacaattc 5881 tctttagcaa gcgaagcgag cgaatcaaac ggagaagcag aagcccattt tattcatttg 5941 aaaaatacaa agatttactg tggagacagt aaatctactc cttctaaagg caaaatcttt 6001 tggagaggga aaatagcaga agtagacggg tttttcttag gaaagatttc tgatgcaaaa 6061 tcaacgagta aaaagagttc ataatactag agccaggcat caaataaaac gaaaggctca 6121 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag 6181 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a //