##gff-version 3
##sequence-region BBa_I20282 1 6221
BBa_I20282	.	engineered_region	21	6084	.	+	0	ID=BBa_I750016;Name=BBa_I750016
BBa_I20282	.	CDS	381	647	.	+	0	ID=annotation1951684;Name=gvpR;Parent=BBa_I750016
BBa_I20282	.	CDS	2384	2652	.	+	0	ID=annotation1951687;Name=gvpG;Parent=BBa_I750016
BBa_I20282	.	point_mutation	738	738	.	+	0	ID=annotation1954599;Name=c->a;Parent=BBa_I750016
BBa_I20282	.	CDS	3880	4167	.	+	0	ID=annotation1951689;Name=gvpS;Parent=BBa_I750016
BBa_I20282	.	point_mutation	2723	2723	.	+	0	ID=annotation1954600;Name=t->c;Parent=BBa_I750016
BBa_I20282	.	point_mutation	3348	3348	.	+	0	ID=annotation1951698;Name=gvpL g696a;Parent=BBa_I750016
BBa_I20282	.	sequence_alteration	3242	3242	.	+	0	ID=annotation1954602;Name=t->a;Parent=BBa_I750016
BBa_I20282	.	point_mutation	2792	2792	.	+	0	ID=annotation1954601;Name=t->c;Parent=BBa_I750016
BBa_I20282	.	point_mutation	4552	4552	.	+	0	ID=annotation1954603;Name=t->a;Parent=BBa_I750016
BBa_I20282	.	sequence_feature	3324	3363	.	+	0	ID=annotation1954616;Name=First copy of 40bp section;Parent=BBa_I750016
BBa_I20282	.	CDS	4766	5644	.	+	0	ID=annotation1951692;Name=gvpT;Parent=BBa_I750016
BBa_I20282	.	point_mutation	3003	3003	.	+	0	ID=annotation1951697;Name=gvpL g351a;Parent=BBa_I750016
BBa_I20282	.	point_mutation	2970	2970	.	+	0	ID=annotation1951696;Name=gvpL g318a;Parent=BBa_I750016
BBa_I20282	.	CDS	676	1603	.	+	0	ID=annotation1951685;Name=gvpN;Parent=BBa_I750016
BBa_I20282	.	CDS	2653	3862	.	+	0	ID=annotation1951688;Name=gvpL;Parent=BBa_I750016
BBa_I20282	.	CDS	5668	6084	.	+	0	ID=annotation1951693;Name=gvpU;Parent=BBa_I750016
BBa_I20282	.	CDS	4121	4405	.	+	0	ID=annotation1951690;Name=gvpK;Parent=BBa_I750016
BBa_I20282	.	point_mutation	5153	5153	.	+	0	ID=annotation1954605;Name=g->a;Parent=BBa_I750016
BBa_I20282	.	CDS	4427	4729	.	+	0	ID=annotation1951691;Name=gvpJ;Parent=BBa_I750016
BBa_I20282	.	point_mutation	2865	2865	.	+	0	ID=annotation1951694;Name=gvpL g213a;Parent=BBa_I750016
BBa_I20282	.	CDS	39	305	.	+	0	ID=annotation1951683;Name=gvpB;Parent=BBa_I750016
BBa_I20282	.	sequence_feature	3364	3763	.	+	0	ID=annotation1954598;Name=10 repeats of 40bp induced durring mutagenesis;Parent=BBa_I750016
BBa_I20282	.	ribosome_entry_site	21	32	.	+	0	ID=annotation1951682;Name=b0034;Parent=BBa_I750016
BBa_I20282	.	point_mutation	5090	5090	.	+	0	ID=annotation1954604;Name=g->a;Parent=BBa_I750016
BBa_I20282	.	CDS	1617	2384	.	+	0	ID=annotation1951686;Name=gvpF;Parent=BBa_I750016
BBa_I20282	.	terminator	6093	6172	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I20282	.	engineered_region	6093	6172	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I20282	.	stem_loop	6104	6147	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I20282	.	terminator	6181	6221	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I20282	.	stop_codon	6214	6214	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I20282	.	polyA_site	6208	6221	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I20282	.	stem_loop	6188	6207	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I20282	.	engineered_region	6181	6221	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I20282	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I20282	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_I20282
aaagaggagaaatactagagaaagaggagaaatactagatgtctattcaaaaaagtactaatagttcaagtttagcaga
agtcattgaccgtattttagataaaggaattgttattgatgcttttgcaagagtttctgttgtaggaattgaaatttta
acgattgaagcgcgagtggttattgccagtgttgatacatggttacgctatgcagaagcagtagggcttcttcgtgacg
acgtagaagaaaacggtcttcctgaacgttcaaattcaagtgaagggcagccgcgttttagtatttaatatgaagtggt
atggaaccttccttttttgagggtggttccagcttttagatctaactattggaggctactaaaaatggaaattaaaaaa
attatgcaagccgtgaacgactttttcggtgaacacgtagctcctcctcataaaattacctcggtggaagctactgaag
atgaaggttggagagttattgttgaagtcattgaagaacgagaatatatgaaaaaatacgccaaagatgaaatgctcgg
aacgtacgagtgctttgtaaataaagaaaaagaagtcatttcattcaaacgactcgacgtcagatatagaagcgccatt
ggcattgaagcataaacgataagatggcaggaggaacgtaaaaatgaccgtcttaacagacaaaaggaaaaaaggcagt
ggagcttttatacaagatgacgagacaaaagaggttctttcaagagcgctgagctatttaaaatccggctattccattc
attttacaggtcctgccggcggaggcaaaacctctttagcgcgagcgcttgctaaaaagagaaagcgtcctgtaatgct
gatgcacgggaatcacgagctcaacaacaaagatttaattggcgattttacgggatacacgagcaaaaaagtaatcgac
cagtacgttcgttctgtctataaaaaagatgaacaggtgagtgaaaactggcaggatggccgattgcttgaagctgtaa
aaaatggctatacgctgatttacgacgaatttactcgttctaagcctgcgacgaataatatctttctatcgatattaga
agaaggcgtgctgccgctgtatggagtaaaaatgaccgatccttttgtgcgcgtgcatcccgatttccgcgtcatcttc
acaagcaatccagctgagtatgccggcgtatatgatacgcaagatgcgcttctcgacaggttaattaccatgtttattg
attataaagacatcgacagagagacagcgattttaacggagaaaacggacgtagaagaagatgaagcgcgcacaattgt
aacgctcgtagcaaacgtgcgaaaccgctctggagacgaaaacagcagcggacttagcctgcgggcttcgcttatgatc
gctacccttgccacgcagcaagacattcctatcgatggaagtgacgaagattttcaaacgttatgtatcgatattttgc
atcatccgcttaccaaatgtttggatgaagaaaatgcaaaaagcaaagccgaaaaaatcattttagaagaatgtaagaa
tatagacactgaagaaaagtaaaggagcttgaaaacatgagtgaaacaaacgaaacaggtatttatatttttagcgcca
ttcaaacggataaagacgaagaatttggcgccgtggaagtagaaggaacaaaagctgaaacatttttgattcgctacaa
agacgcggctatggtagcagctgaagtaccgatgaaaatttatcatcctaatcgccaaaatttattaatgcatcaaaac
gcagtagcagcgattatggacaagaacgatacggttattccaatcagctttgggaatgtattcaaatcaaaagaagacg
taaaagttcttttggaaaacctttatccgcagtttgaaaagctgtttccagcgatcaaaggaaaaattgaagtcggttt
aaaagtaattgggaaaaaagaatggcttgagaaaaaagtaaacgaaaatcctgaacttgagaaagtatcagcatccgta
aaaggaaaatcagaagcagccggttattatgagcgtattcaacttggaggaatggctcaaaagatgtttacttccctgc
aaaaagaagtcaagacagatgtgttttctccgcttgaagaagcagcggaagcagcaaaagcaaatgagccaacgggcga
aacgatgcttttaaacgcgtctttcttaattaaccgagaagatgaagcgaagtttgatgaaaaagtaaatgaagcgcat
gaaaactggaaagacaaagccgattttcattacagcggtccttggcctgcttataattttgtgaacattcgcctaaaag
tagaagagaaataacgtgcttcacaaattagtaaccgcacccattaaccttgtagtgaaaatcggcgaaaaagtacagg
aagaagctgataaacagctatatgaccttccgacgattcagcaaaagctcattcagcttcaaatgatgtttgagcttgg
tgaaattccagaagaagcgtttcaagaaaaagaagatgaattgttaatgaggtacgaaattgcgaaacgcagagaaatt
gaacaatgggaagagctaacacaaaaaagaaatgaggaatcctagatgggagaattactgtatttatacggtttaattc
caacaaaagaagcagcagccatagagccgtttccatcttataaggggtttgacggagaacattcactgtacccaattgc
gtttgatcaggtgacggctgtagtttctaagctggatgctgacacctattcagaaaaagtgattcaagaaaaaatggag
caggatatgagctggctgcaagaaaaagcatttcatcatcacgaaacggtagccgctttgtacgaagaatttacgatca
ttccattaaaattttgcaccatttataaaggtgaagaaagtctgcaagcagctattgagattaacaaagaaaagataga
aaattcactgacgctgcttcaaggaaatgaagagtggaatgtgaaaatttactgtgatgatacagagcttaaaaaagga
atcagcgaaacgaatgaaagcgtgaaagcgaaaaaacaagaaattagtcacttatcaccaggaagacagttttttgaaa
agaaaaaaatagatcagctgattgaaaaagaattagagcttcacaaaaacaaagtgtgtgaagagatacatgacaagct
aaaagaattatcgctttatgactctgttaaaaagaattggagcaaagacgtaactggcgcagctgaacagatggcgtgg
aacagcgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaag
ttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaa
gttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattacta
agttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattact
aagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattac
taagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtaaacgaaatagaagagcttcagcaaaggct
tgaaaataaaggctggaagtttgaagtgacgggaccatggccgccctatcatttctcgagctttgcgtaaagtgaggaa
ttaacattatgtctcttaaacaatccatggagaataaagatattgctcttattgatattttagatgtcattttagataa
aggagtcgccattaaaggagacttaatcatttccatagctggcgtcgatttagtgtatttggatttgcgggtgcttatt
tcttcggttgaaacgcttgtgcaagcaaaagaaggaaatcacaaaccaatcacttctgaacaatttgataaacaaaagg
aggaattaatggatgcaaccggtcagccaagcaaatggacgaatccacttggatcctgatcaagctgaacaaggcttag
cgcagcttgtgatgacagttattgagctattgaggcaaatcgttgaacgtcatgccatgaggcgggtggagggtggaac
gttgacggacgaacaaattgaaaacttaggaattgcactaatgaacttagaagaaaaaatggacgagttgaaagaggtg
ttcggtctggatgcagaagatttaaatattgatcttggaccgctaggcagcctgctttaagcggtcagtaggaggaaca
gtatggcagtcgaacataatatgcagtcaagtacgattgtagatgtgctcgaaaagattttggataaaggagtcgttat
agcgggggacatcaccgtaggaattgcagatgtcgagctattaacgataaagatccgcttgattgtggcttcggttgat
aaggcaaaagaaatcggcatggactggtgggaaaatgatccgtatctcagttcaaaaggagccaataacaaagcgctcg
aagaagaaaataaaatgctgcatgagcggttaaaaacgcttgaagaaaaaatagaaacgaaacgttaaaaactgtacgc
tacttaaaaaatggagggatttacaatggcaactgaaacaaaattagataacacacaggcagaaaacaaggaaaataaa
aatgcggaaaacggttcaaaagaaaagaacggttcaaaagcaagcaaaacaacaagcagcgggccaatcaaacgagcgg
tagcaggaggcatcatcggtgcaacgattggatatgtatcgactcctgaaaatcgaaaaagtctccttgaccgcattga
tacagacgaattaaaaagcaaagcatctgatttaggaacaaaggtaaaagaaaaatcaaaaagcagcgtggccagcctg
aaaacatctgcgggaagcttgtttaaaaaagataaagataaatcaaaagatgatgaagaaaacgtaaattcttctagta
gcgaaacagaagacgataacgttcaagagtacgacgagttaaaagaagaaaatcaaactcttcaagatcgcttatcaca
gcttgaagaaaaaatgaacatgcttgttgagcttagcctcaataaaaatcaagacgaagaagcggaagatacagattcc
gacgaagaagagaacgatgagaacgatgaaaacgatgaaaacgagcaggacgatgaaaacgaagaagaaacatctaagc
cacgtaaaaaggataaaaaagaagctgaggaagaagaaagtgaaagtgacgaagacagcgaggaagaagaggaagattc
tcgctcaaacaaaaaaaataaaaaagtaaaaacagaagaagaagacgaagatgaaagcgaagaagaaaaaaaggaagcg
aaaccaaaaaagtcaacagctaaaaaatcaaaaaatacaaaagcaaagaaaaacacggacgaagaagatgatgaagcaa
catctctttctagtgaagacgatacaacagcctaagacgtaaaggaggaaagaaagacatgagtacaggcccttctttt
tcaactaaagacaatacgcttgaatactttgtgaaagcttctaataaacacggcttttcacttgatatttcattaaatg
taaacggcgctgtgatttccggtaccatgatttcagcaaaagaatactttgattacttaagcgaaacgtttgaagaagg
cagtgaagtggctcaggcgctaagcgaacaattctctttagcaagcgaagcgagcgaatcaaacggagaagcagaagcc
cattttattcatttgaaaaatacaaagatttactgtggagacagtaaatctactccttctaaaggcaaaatcttttgga
gagggaaaatagcagaagtagacgggtttttcttaggaaagatttctgatgcaaaatcaacgagtaaaaagagttcata
atactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggt
gaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata