BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_I750016 1 BBa_I750016 Gas Vesicle polycistonic gene 2007-10-20T11:00:00Z 2015-08-31T04:08:05Z This 5.7Kbp part contains 11 open reading frames coding for gas vesicle genes (gvpB,R,N,F,G,L,S,K,J,T and U) from Bacillus megaterium VT1660 AF053765. Original AF053765 DNA in pBluescriptIIKS plasmid (pNL29 in "Gas Vesicle genes identified in Bacillus megaterium and Functional Expression in Escherichia coli, Ning Li and Maura C. Cannon Jounal of bacteriology May 1998 p2450-2458). This 5.7Kbp part contains 11 open reading frames coding for gas vesicle genes (gvpB,R,N,F,G,L,S,K,J,T and U) from Bacillus megaterium VT1660 AF053765. Promotion of the sequence results in expression of gas vesicles which slow the rate of settling of the cells in Saline. false false _132_ 0 1721 9 It's complicated false Site directed mutagenesis was performed in four rounds to remove 3 PstI sites and one EcoRI site from gvpL in the sequence. Primers with biobrick prefix and suffix were then used in a PCR reaction to amplify the part. Finally BBa_J61035 was used to provide the plasmid backbone which introduced a RBS at the front of the part. true Phillip Dodson annotation1951693 1 gvpU range1951693 1 5648 6064 annotation1951691 1 gvpJ range1951691 1 4407 4709 annotation1951696 1 gvpL g318a range1951696 1 2950 2950 annotation1951697 1 gvpL g351a range1951697 1 2983 2983 annotation1951685 1 gvpN range1951685 1 656 1583 annotation1951692 1 gvpT range1951692 1 4746 5624 annotation1954598 1 10 repeats of 40bp induced durring mutagenesis range1954598 1 3344 3743 annotation1951687 1 gvpG range1951687 1 2364 2632 annotation1951694 1 gvpL g213a range1951694 1 2845 2845 annotation1954616 1 First copy of 40bp section range1954616 1 3304 3343 annotation1954601 1 t->c range1954601 1 2772 2772 annotation1951690 1 gvpK range1951690 1 4101 4385 annotation1951684 1 gvpR range1951684 1 361 627 annotation1954600 1 t->c range1954600 1 2703 2703 annotation1951689 1 gvpS range1951689 1 3860 4147 annotation1954602 1 t->a range1954602 1 3222 3222 annotation1954603 1 t->a range1954603 1 4532 4532 annotation1954604 1 g->a range1954604 1 5070 5070 annotation1954605 1 g->a range1954605 1 5133 5133 annotation1951683 1 gvpB range1951683 1 19 285 annotation1951682 1 b0034 range1951682 1 1 12 annotation1951688 1 gvpL range1951688 1 2633 3842 annotation1954599 1 c->a range1954599 1 718 718 annotation1951698 1 gvpL g696a range1951698 1 3328 3328 annotation1951686 1 gvpF range1951686 1 1597 2364 BBa_I20282 1 BBa_I20282 Protein Balloon Generator 2008-05-03T11:00:00Z 2015-08-31T04:07:40Z 2007 Melbourne iGEM team and Maura Cannon lab at UMass Amherst The device receives a PoPS-input signal and produces a protein balloon that can changes the buoyancy of a cellular chassis. false false _11_ 0 52 167 Not in stock false n/a false Drew Endy component1962322 1 BBa_B0010 component1962324 1 BBa_B0012 component1962321 1 BBa_I750016 component1962295 1 BBa_B0034 annotation1962321 1 BBa_I750016 range1962321 1 21 6084 annotation1962322 1 BBa_B0010 range1962322 1 6093 6172 annotation1962295 1 BBa_B0034 range1962295 1 1 12 annotation1962324 1 BBa_B0012 range1962324 1 6181 6221 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_I20282_sequence 1 aaagaggagaaatactagagaaagaggagaaatactagatgtctattcaaaaaagtactaatagttcaagtttagcagaagtcattgaccgtattttagataaaggaattgttattgatgcttttgcaagagtttctgttgtaggaattgaaattttaacgattgaagcgcgagtggttattgccagtgttgatacatggttacgctatgcagaagcagtagggcttcttcgtgacgacgtagaagaaaacggtcttcctgaacgttcaaattcaagtgaagggcagccgcgttttagtatttaatatgaagtggtatggaaccttccttttttgagggtggttccagcttttagatctaactattggaggctactaaaaatggaaattaaaaaaattatgcaagccgtgaacgactttttcggtgaacacgtagctcctcctcataaaattacctcggtggaagctactgaagatgaaggttggagagttattgttgaagtcattgaagaacgagaatatatgaaaaaatacgccaaagatgaaatgctcggaacgtacgagtgctttgtaaataaagaaaaagaagtcatttcattcaaacgactcgacgtcagatatagaagcgccattggcattgaagcataaacgataagatggcaggaggaacgtaaaaatgaccgtcttaacagacaaaaggaaaaaaggcagtggagcttttatacaagatgacgagacaaaagaggttctttcaagagcgctgagctatttaaaatccggctattccattcattttacaggtcctgccggcggaggcaaaacctctttagcgcgagcgcttgctaaaaagagaaagcgtcctgtaatgctgatgcacgggaatcacgagctcaacaacaaagatttaattggcgattttacgggatacacgagcaaaaaagtaatcgaccagtacgttcgttctgtctataaaaaagatgaacaggtgagtgaaaactggcaggatggccgattgcttgaagctgtaaaaaatggctatacgctgatttacgacgaatttactcgttctaagcctgcgacgaataatatctttctatcgatattagaagaaggcgtgctgccgctgtatggagtaaaaatgaccgatccttttgtgcgcgtgcatcccgatttccgcgtcatcttcacaagcaatccagctgagtatgccggcgtatatgatacgcaagatgcgcttctcgacaggttaattaccatgtttattgattataaagacatcgacagagagacagcgattttaacggagaaaacggacgtagaagaagatgaagcgcgcacaattgtaacgctcgtagcaaacgtgcgaaaccgctctggagacgaaaacagcagcggacttagcctgcgggcttcgcttatgatcgctacccttgccacgcagcaagacattcctatcgatggaagtgacgaagattttcaaacgttatgtatcgatattttgcatcatccgcttaccaaatgtttggatgaagaaaatgcaaaaagcaaagccgaaaaaatcattttagaagaatgtaagaatatagacactgaagaaaagtaaaggagcttgaaaacatgagtgaaacaaacgaaacaggtatttatatttttagcgccattcaaacggataaagacgaagaatttggcgccgtggaagtagaaggaacaaaagctgaaacatttttgattcgctacaaagacgcggctatggtagcagctgaagtaccgatgaaaatttatcatcctaatcgccaaaatttattaatgcatcaaaacgcagtagcagcgattatggacaagaacgatacggttattccaatcagctttgggaatgtattcaaatcaaaagaagacgtaaaagttcttttggaaaacctttatccgcagtttgaaaagctgtttccagcgatcaaaggaaaaattgaagtcggtttaaaagtaattgggaaaaaagaatggcttgagaaaaaagtaaacgaaaatcctgaacttgagaaagtatcagcatccgtaaaaggaaaatcagaagcagccggttattatgagcgtattcaacttggaggaatggctcaaaagatgtttacttccctgcaaaaagaagtcaagacagatgtgttttctccgcttgaagaagcagcggaagcagcaaaagcaaatgagccaacgggcgaaacgatgcttttaaacgcgtctttcttaattaaccgagaagatgaagcgaagtttgatgaaaaagtaaatgaagcgcatgaaaactggaaagacaaagccgattttcattacagcggtccttggcctgcttataattttgtgaacattcgcctaaaagtagaagagaaataacgtgcttcacaaattagtaaccgcacccattaaccttgtagtgaaaatcggcgaaaaagtacaggaagaagctgataaacagctatatgaccttccgacgattcagcaaaagctcattcagcttcaaatgatgtttgagcttggtgaaattccagaagaagcgtttcaagaaaaagaagatgaattgttaatgaggtacgaaattgcgaaacgcagagaaattgaacaatgggaagagctaacacaaaaaagaaatgaggaatcctagatgggagaattactgtatttatacggtttaattccaacaaaagaagcagcagccatagagccgtttccatcttataaggggtttgacggagaacattcactgtacccaattgcgtttgatcaggtgacggctgtagtttctaagctggatgctgacacctattcagaaaaagtgattcaagaaaaaatggagcaggatatgagctggctgcaagaaaaagcatttcatcatcacgaaacggtagccgctttgtacgaagaatttacgatcattccattaaaattttgcaccatttataaaggtgaagaaagtctgcaagcagctattgagattaacaaagaaaagatagaaaattcactgacgctgcttcaaggaaatgaagagtggaatgtgaaaatttactgtgatgatacagagcttaaaaaaggaatcagcgaaacgaatgaaagcgtgaaagcgaaaaaacaagaaattagtcacttatcaccaggaagacagttttttgaaaagaaaaaaatagatcagctgattgaaaaagaattagagcttcacaaaaacaaagtgtgtgaagagatacatgacaagctaaaagaattatcgctttatgactctgttaaaaagaattggagcaaagacgtaactggcgcagctgaacagatggcgtggaacagcgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtaaacgaaatagaagagcttcagcaaaggcttgaaaataaaggctggaagtttgaagtgacgggaccatggccgccctatcatttctcgagctttgcgtaaagtgaggaattaacattatgtctcttaaacaatccatggagaataaagatattgctcttattgatattttagatgtcattttagataaaggagtcgccattaaaggagacttaatcatttccatagctggcgtcgatttagtgtatttggatttgcgggtgcttatttcttcggttgaaacgcttgtgcaagcaaaagaaggaaatcacaaaccaatcacttctgaacaatttgataaacaaaaggaggaattaatggatgcaaccggtcagccaagcaaatggacgaatccacttggatcctgatcaagctgaacaaggcttagcgcagcttgtgatgacagttattgagctattgaggcaaatcgttgaacgtcatgccatgaggcgggtggagggtggaacgttgacggacgaacaaattgaaaacttaggaattgcactaatgaacttagaagaaaaaatggacgagttgaaagaggtgttcggtctggatgcagaagatttaaatattgatcttggaccgctaggcagcctgctttaagcggtcagtaggaggaacagtatggcagtcgaacataatatgcagtcaagtacgattgtagatgtgctcgaaaagattttggataaaggagtcgttatagcgggggacatcaccgtaggaattgcagatgtcgagctattaacgataaagatccgcttgattgtggcttcggttgataaggcaaaagaaatcggcatggactggtgggaaaatgatccgtatctcagttcaaaaggagccaataacaaagcgctcgaagaagaaaataaaatgctgcatgagcggttaaaaacgcttgaagaaaaaatagaaacgaaacgttaaaaactgtacgctacttaaaaaatggagggatttacaatggcaactgaaacaaaattagataacacacaggcagaaaacaaggaaaataaaaatgcggaaaacggttcaaaagaaaagaacggttcaaaagcaagcaaaacaacaagcagcgggccaatcaaacgagcggtagcaggaggcatcatcggtgcaacgattggatatgtatcgactcctgaaaatcgaaaaagtctccttgaccgcattgatacagacgaattaaaaagcaaagcatctgatttaggaacaaaggtaaaagaaaaatcaaaaagcagcgtggccagcctgaaaacatctgcgggaagcttgtttaaaaaagataaagataaatcaaaagatgatgaagaaaacgtaaattcttctagtagcgaaacagaagacgataacgttcaagagtacgacgagttaaaagaagaaaatcaaactcttcaagatcgcttatcacagcttgaagaaaaaatgaacatgcttgttgagcttagcctcaataaaaatcaagacgaagaagcggaagatacagattccgacgaagaagagaacgatgagaacgatgaaaacgatgaaaacgagcaggacgatgaaaacgaagaagaaacatctaagccacgtaaaaaggataaaaaagaagctgaggaagaagaaagtgaaagtgacgaagacagcgaggaagaagaggaagattctcgctcaaacaaaaaaaataaaaaagtaaaaacagaagaagaagacgaagatgaaagcgaagaagaaaaaaaggaagcgaaaccaaaaaagtcaacagctaaaaaatcaaaaaatacaaaagcaaagaaaaacacggacgaagaagatgatgaagcaacatctctttctagtgaagacgatacaacagcctaagacgtaaaggaggaaagaaagacatgagtacaggcccttctttttcaactaaagacaatacgcttgaatactttgtgaaagcttctaataaacacggcttttcacttgatatttcattaaatgtaaacggcgctgtgatttccggtaccatgatttcagcaaaagaatactttgattacttaagcgaaacgtttgaagaaggcagtgaagtggctcaggcgctaagcgaacaattctctttagcaagcgaagcgagcgaatcaaacggagaagcagaagcccattttattcatttgaaaaatacaaagatttactgtggagacagtaaatctactccttctaaaggcaaaatcttttggagagggaaaatagcagaagtagacgggtttttcttaggaaagatttctgatgcaaaatcaacgagtaaaaagagttcataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0034_sequence 1 aaagaggagaaa BBa_I750016_sequence 1 aaagaggagaaatactagatgtctattcaaaaaagtactaatagttcaagtttagcagaagtcattgaccgtattttagataaaggaattgttattgatgcttttgcaagagtttctgttgtaggaattgaaattttaacgattgaagcgcgagtggttattgccagtgttgatacatggttacgctatgcagaagcagtagggcttcttcgtgacgacgtagaagaaaacggtcttcctgaacgttcaaattcaagtgaagggcagccgcgttttagtatttaatatgaagtggtatggaaccttccttttttgagggtggttccagcttttagatctaactattggaggctactaaaaatggaaattaaaaaaattatgcaagccgtgaacgactttttcggtgaacacgtagctcctcctcataaaattacctcggtggaagctactgaagatgaaggttggagagttattgttgaagtcattgaagaacgagaatatatgaaaaaatacgccaaagatgaaatgctcggaacgtacgagtgctttgtaaataaagaaaaagaagtcatttcattcaaacgactcgacgtcagatatagaagcgccattggcattgaagcataaacgataagatggcaggaggaacgtaaaaatgaccgtcttaacagacaaaaggaaaaaaggcagtggagcttttatacaagatgacgagacaaaagaggttctttcaagagcgctgagctatttaaaatccggctattccattcattttacaggtcctgccggcggaggcaaaacctctttagcgcgagcgcttgctaaaaagagaaagcgtcctgtaatgctgatgcacgggaatcacgagctcaacaacaaagatttaattggcgattttacgggatacacgagcaaaaaagtaatcgaccagtacgttcgttctgtctataaaaaagatgaacaggtgagtgaaaactggcaggatggccgattgcttgaagctgtaaaaaatggctatacgctgatttacgacgaatttactcgttctaagcctgcgacgaataatatctttctatcgatattagaagaaggcgtgctgccgctgtatggagtaaaaatgaccgatccttttgtgcgcgtgcatcccgatttccgcgtcatcttcacaagcaatccagctgagtatgccggcgtatatgatacgcaagatgcgcttctcgacaggttaattaccatgtttattgattataaagacatcgacagagagacagcgattttaacggagaaaacggacgtagaagaagatgaagcgcgcacaattgtaacgctcgtagcaaacgtgcgaaaccgctctggagacgaaaacagcagcggacttagcctgcgggcttcgcttatgatcgctacccttgccacgcagcaagacattcctatcgatggaagtgacgaagattttcaaacgttatgtatcgatattttgcatcatccgcttaccaaatgtttggatgaagaaaatgcaaaaagcaaagccgaaaaaatcattttagaagaatgtaagaatatagacactgaagaaaagtaaaggagcttgaaaacatgagtgaaacaaacgaaacaggtatttatatttttagcgccattcaaacggataaagacgaagaatttggcgccgtggaagtagaaggaacaaaagctgaaacatttttgattcgctacaaagacgcggctatggtagcagctgaagtaccgatgaaaatttatcatcctaatcgccaaaatttattaatgcatcaaaacgcagtagcagcgattatggacaagaacgatacggttattccaatcagctttgggaatgtattcaaatcaaaagaagacgtaaaagttcttttggaaaacctttatccgcagtttgaaaagctgtttccagcgatcaaaggaaaaattgaagtcggtttaaaagtaattgggaaaaaagaatggcttgagaaaaaagtaaacgaaaatcctgaacttgagaaagtatcagcatccgtaaaaggaaaatcagaagcagccggttattatgagcgtattcaacttggaggaatggctcaaaagatgtttacttccctgcaaaaagaagtcaagacagatgtgttttctccgcttgaagaagcagcggaagcagcaaaagcaaatgagccaacgggcgaaacgatgcttttaaacgcgtctttcttaattaaccgagaagatgaagcgaagtttgatgaaaaagtaaatgaagcgcatgaaaactggaaagacaaagccgattttcattacagcggtccttggcctgcttataattttgtgaacattcgcctaaaagtagaagagaaataacgtgcttcacaaattagtaaccgcacccattaaccttgtagtgaaaatcggcgaaaaagtacaggaagaagctgataaacagctatatgaccttccgacgattcagcaaaagctcattcagcttcaaatgatgtttgagcttggtgaaattccagaagaagcgtttcaagaaaaagaagatgaattgttaatgaggtacgaaattgcgaaacgcagagaaattgaacaatgggaagagctaacacaaaaaagaaatgaggaatcctagatgggagaattactgtatttatacggtttaattccaacaaaagaagcagcagccatagagccgtttccatcttataaggggtttgacggagaacattcactgtacccaattgcgtttgatcaggtgacggctgtagtttctaagctggatgctgacacctattcagaaaaagtgattcaagaaaaaatggagcaggatatgagctggctgcaagaaaaagcatttcatcatcacgaaacggtagccgctttgtacgaagaatttacgatcattccattaaaattttgcaccatttataaaggtgaagaaagtctgcaagcagctattgagattaacaaagaaaagatagaaaattcactgacgctgcttcaaggaaatgaagagtggaatgtgaaaatttactgtgatgatacagagcttaaaaaaggaatcagcgaaacgaatgaaagcgtgaaagcgaaaaaacaagaaattagtcacttatcaccaggaagacagttttttgaaaagaaaaaaatagatcagctgattgaaaaagaattagagcttcacaaaaacaaagtgtgtgaagagatacatgacaagctaaaagaattatcgctttatgactctgttaaaaagaattggagcaaagacgtaactggcgcagctgaacagatggcgtggaacagcgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtacgtgtttcttctcccgtctctgcaaattactaagttcgtaaacgaaatagaagagcttcagcaaaggcttgaaaataaaggctggaagtttgaagtgacgggaccatggccgccctatcatttctcgagctttgcgtaaagtgaggaattaacattatgtctcttaaacaatccatggagaataaagatattgctcttattgatattttagatgtcattttagataaaggagtcgccattaaaggagacttaatcatttccatagctggcgtcgatttagtgtatttggatttgcgggtgcttatttcttcggttgaaacgcttgtgcaagcaaaagaaggaaatcacaaaccaatcacttctgaacaatttgataaacaaaaggaggaattaatggatgcaaccggtcagccaagcaaatggacgaatccacttggatcctgatcaagctgaacaaggcttagcgcagcttgtgatgacagttattgagctattgaggcaaatcgttgaacgtcatgccatgaggcgggtggagggtggaacgttgacggacgaacaaattgaaaacttaggaattgcactaatgaacttagaagaaaaaatggacgagttgaaagaggtgttcggtctggatgcagaagatttaaatattgatcttggaccgctaggcagcctgctttaagcggtcagtaggaggaacagtatggcagtcgaacataatatgcagtcaagtacgattgtagatgtgctcgaaaagattttggataaaggagtcgttatagcgggggacatcaccgtaggaattgcagatgtcgagctattaacgataaagatccgcttgattgtggcttcggttgataaggcaaaagaaatcggcatggactggtgggaaaatgatccgtatctcagttcaaaaggagccaataacaaagcgctcgaagaagaaaataaaatgctgcatgagcggttaaaaacgcttgaagaaaaaatagaaacgaaacgttaaaaactgtacgctacttaaaaaatggagggatttacaatggcaactgaaacaaaattagataacacacaggcagaaaacaaggaaaataaaaatgcggaaaacggttcaaaagaaaagaacggttcaaaagcaagcaaaacaacaagcagcgggccaatcaaacgagcggtagcaggaggcatcatcggtgcaacgattggatatgtatcgactcctgaaaatcgaaaaagtctccttgaccgcattgatacagacgaattaaaaagcaaagcatctgatttaggaacaaaggtaaaagaaaaatcaaaaagcagcgtggccagcctgaaaacatctgcgggaagcttgtttaaaaaagataaagataaatcaaaagatgatgaagaaaacgtaaattcttctagtagcgaaacagaagacgataacgttcaagagtacgacgagttaaaagaagaaaatcaaactcttcaagatcgcttatcacagcttgaagaaaaaatgaacatgcttgttgagcttagcctcaataaaaatcaagacgaagaagcggaagatacagattccgacgaagaagagaacgatgagaacgatgaaaacgatgaaaacgagcaggacgatgaaaacgaagaagaaacatctaagccacgtaaaaaggataaaaaagaagctgaggaagaagaaagtgaaagtgacgaagacagcgaggaagaagaggaagattctcgctcaaacaaaaaaaataaaaaagtaaaaacagaagaagaagacgaagatgaaagcgaagaagaaaaaaaggaagcgaaaccaaaaaagtcaacagctaaaaaatcaaaaaatacaaaagcaaagaaaaacacggacgaagaagatgatgaagcaacatctctttctagtgaagacgatacaacagcctaagacgtaaaggaggaaagaaagacatgagtacaggcccttctttttcaactaaagacaatacgcttgaatactttgtgaaagcttctaataaacacggcttttcacttgatatttcattaaatgtaaacggcgctgtgatttccggtaccatgatttcagcaaaagaatactttgattacttaagcgaaacgtttgaagaaggcagtgaagtggctcaggcgctaagcgaacaattctctttagcaagcgaagcgagcgaatcaaacggagaagcagaagcccattttattcatttgaaaaatacaaagatttactgtggagacagtaaatctactccttctaaaggcaaaatcttttggagagggaaaatagcagaagtagacgggtttttcttaggaaagatttctgatgcaaaatcaacgagtaaaaagagttcataa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z