BBa_I20282
type: ComponentDefinition
persistent identity: BBa I20282
display id: BBa_I20282
version: 1
was generated by: iGEM to SBOL conversion
description: Protein Balloon Generator
created: 2008-05-03T11:00:00Z
modified: 2015-08-31T04:07:40Z
mutable provenance: 2007 Melbourne iGEM team and Maura Cannon lab at UMass Amherst
owned by: james
owned by: myers
mutable description: The device receives a PoPS-input signal and produces a protein balloon that can changes the buoyancy of a cellular chassis.
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 52
owning group id: 167
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: n/a
star: false
creator: Drew Endy
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_B0034
component: BBa_B0010
component: BBa_I750016
component: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_I750016
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I20282_sequence/1

BBa_B0034
type: Component
persistent identity: component1962295
display id: component1962295
version: 1
top level: BBa_I20282
definition: BBa_B0034
access: public

BBa_B0010
type: Component
persistent identity: component1962322
display id: component1962322
version: 1
top level: BBa_I20282
definition: BBa_B0010
access: public

BBa_I750016
type: Component
persistent identity: component1962321
display id: component1962321
version: 1
top level: BBa_I20282
definition: BBa_I750016
access: public

BBa_B0012
type: Component
persistent identity: component1962324
display id: component1962324
version: 1
top level: BBa_I20282
definition: BBa_B0012
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1962295
display id: annotation1962295
version: 1
top level: BBa_I20282
location: https://synbiohub.org/public/igem/BBa_I20282/annotation1962295/range1962295/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I20282/annotation1962295/range1962295/1
type: Range
persistent identity: range1962295
display id: range1962295
version: 1
top level: BBa_I20282
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_I750016
type: SequenceAnnotation
persistent identity: annotation1962321
display id: annotation1962321
version: 1
top level: BBa_I20282
location: https://synbiohub.org/public/igem/BBa_I20282/annotation1962321/range1962321/1
component: BBa_I750016

https://synbiohub.org/public/igem/BBa_I20282/annotation1962321/range1962321/1
type: Range
persistent identity: range1962321
display id: range1962321
version: 1
top level: BBa_I20282
direction: direction/reverse
start: 21
end: 6084
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1962322
display id: annotation1962322
version: 1
top level: BBa_I20282
location: https://synbiohub.org/public/igem/BBa_I20282/annotation1962322/range1962322/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I20282/annotation1962322/range1962322/1
type: Range
persistent identity: range1962322
display id: range1962322
version: 1
top level: BBa_I20282
direction: direction/forward
start: 6093
end: 6172
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1962324
display id: annotation1962324
version: 1
top level: BBa_I20282
location: https://synbiohub.org/public/igem/BBa_I20282/annotation1962324/range1962324/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I20282/annotation1962324/range1962324/1
type: Range
persistent identity: range1962324
display id: range1962324
version: 1
top level: BBa_I20282
direction: direction/forward
start: 6181
end: 6221
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_I750016
type: ComponentDefinition
persistent identity: BBa I750016
display id: BBa_I750016
version: 1
was generated by: iGEM to SBOL conversion
description: Gas Vesicle polycistonic gene
created: 2007-10-20T11:00:00Z
modified: 2015-08-31T04:08:05Z
mutable provenance: This 5.7Kbp part contains 11 open reading frames coding for gas vesicle genes (gvpB,R,N,F,G,L,S,K,J,T and U) from Bacillus megaterium VT1660 AF053765. Original AF053765 DNA in pBluescriptIIKS plasmid (pNL29 in "Gas Vesicle genes identified in Bacillus megaterium and Functional Expression in Escherichia coli, Ning Li and Maura C. Cannon Jounal of bacteriology May 1998 p2450-2458).
owned by: james
owned by: myers
mutable description: This 5.7Kbp part contains 11 open reading frames coding for gas vesicle genes (gvpB,R,N,F,G,L,S,K,J,T and U) from Bacillus megaterium VT1660 AF053765. Promotion of the sequence results in expression of gas vesicles which slow the rate of settling of the cells in Saline.
discontinued: false
dominant: false
experience: experience/None
group u list: _132_
m user id: 0
owner id: 1721
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Site directed mutagenesis was performed in four rounds to remove 3 PstI sites and one EcoRI site from gvpL in the sequence. Primers with biobrick prefix and suffix were then used in a PCR reaction to amplify the part. Finally BBa_J61035 was used to provide the plasmid backbone which introduced a RBS at the front of the part.
star: true
creator: Phillip Dodson
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: b0034
sequence annotation: gvpB
sequence annotation: gvpR
sequence annotation: gvpN
sequence annotation: c->a
sequence annotation: gvpF
sequence annotation: gvpG
sequence annotation: gvpL
sequence annotation: t->c
sequence annotation: gvpL g213a
sequence annotation: gvpL g318a
sequence annotation: gvpL g351a
sequence annotation: t->a
sequence annotation: First copy of 40bp section
sequence annotation: gvpL g696a
sequence annotation: 10 repeats of 40bp induced durring mutagenesis
sequence annotation: gvpS
sequence annotation: gvpK
sequence annotation: gvpJ
sequence annotation: gvpT
sequence annotation: g->a
sequence annotation: gvpU
sequence: https://synbiohub.org/public/igem/BBa_I750016_sequence/1

b0034
type: SequenceAnnotation
persistent identity: annotation1951682
display id: annotation1951682
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951682/range1951682/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_I750016/annotation1951682/range1951682/1
type: Range
persistent identity: range1951682
display id: range1951682
version: 1
top level: BBa_I750016
direction: direction/forward
start: 1
end: 12
orientation: inline

gvpB
type: SequenceAnnotation
persistent identity: annotation1951683
display id: annotation1951683
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951683/range1951683/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I750016/annotation1951683/range1951683/1
type: Range
persistent identity: range1951683
display id: range1951683
version: 1
top level: BBa_I750016
direction: direction/forward
start: 19
end: 285
orientation: inline

gvpR
type: SequenceAnnotation
persistent identity: annotation1951684
display id: annotation1951684
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951684/range1951684/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I750016/annotation1951684/range1951684/1
type: Range
persistent identity: range1951684
display id: range1951684
version: 1
top level: BBa_I750016
direction: direction/forward
start: 361
end: 627
orientation: inline

gvpN
type: SequenceAnnotation
persistent identity: annotation1951685
display id: annotation1951685
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951685/range1951685/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I750016/annotation1951685/range1951685/1
type: Range
persistent identity: range1951685
display id: range1951685
version: 1
top level: BBa_I750016
direction: direction/forward
start: 656
end: 1583
orientation: inline

c->a
type: SequenceAnnotation
persistent identity: annotation1954599
display id: annotation1954599
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1954599/range1954599/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_I750016/annotation1954599/range1954599/1
type: Range
persistent identity: range1954599
display id: range1954599
version: 1
top level: BBa_I750016
direction: direction/forward
start: 718
end: 718
orientation: inline

gvpF
type: SequenceAnnotation
persistent identity: annotation1951686
display id: annotation1951686
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951686/range1951686/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I750016/annotation1951686/range1951686/1
type: Range
persistent identity: range1951686
display id: range1951686
version: 1
top level: BBa_I750016
direction: direction/forward
start: 1597
end: 2364
orientation: inline

gvpG
type: SequenceAnnotation
persistent identity: annotation1951687
display id: annotation1951687
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951687/range1951687/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I750016/annotation1951687/range1951687/1
type: Range
persistent identity: range1951687
display id: range1951687
version: 1
top level: BBa_I750016
direction: direction/forward
start: 2364
end: 2632
orientation: inline

gvpL
type: SequenceAnnotation
persistent identity: annotation1951688
display id: annotation1951688
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951688/range1951688/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I750016/annotation1951688/range1951688/1
type: Range
persistent identity: range1951688
display id: range1951688
version: 1
top level: BBa_I750016
direction: direction/forward
start: 2633
end: 3842
orientation: inline

t->c
type: SequenceAnnotation
persistent identity: annotation1954600
display id: annotation1954600
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1954600/range1954600/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_I750016/annotation1954600/range1954600/1
type: Range
persistent identity: range1954600
display id: range1954600
version: 1
top level: BBa_I750016
direction: direction/forward
start: 2703
end: 2703
orientation: inline

t->c
type: SequenceAnnotation
persistent identity: annotation1954601
display id: annotation1954601
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1954601/range1954601/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_I750016/annotation1954601/range1954601/1
type: Range
persistent identity: range1954601
display id: range1954601
version: 1
top level: BBa_I750016
direction: direction/forward
start: 2772
end: 2772
orientation: inline

gvpL g213a
type: SequenceAnnotation
persistent identity: annotation1951694
display id: annotation1951694
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951694/range1951694/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_I750016/annotation1951694/range1951694/1
type: Range
persistent identity: range1951694
display id: range1951694
version: 1
top level: BBa_I750016
direction: direction/forward
start: 2845
end: 2845
orientation: inline

gvpL g318a
type: SequenceAnnotation
persistent identity: annotation1951696
display id: annotation1951696
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951696/range1951696/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_I750016/annotation1951696/range1951696/1
type: Range
persistent identity: range1951696
display id: range1951696
version: 1
top level: BBa_I750016
direction: direction/forward
start: 2950
end: 2950
orientation: inline

gvpL g351a
type: SequenceAnnotation
persistent identity: annotation1951697
display id: annotation1951697
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951697/range1951697/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_I750016/annotation1951697/range1951697/1
type: Range
persistent identity: range1951697
display id: range1951697
version: 1
top level: BBa_I750016
direction: direction/forward
start: 2983
end: 2983
orientation: inline

t->a
type: SequenceAnnotation
persistent identity: annotation1954602
display id: annotation1954602
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1954602/range1954602/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_I750016/annotation1954602/range1954602/1
type: Range
persistent identity: range1954602
display id: range1954602
version: 1
top level: BBa_I750016
direction: direction/forward
start: 3222
end: 3222
orientation: inline

First copy of 40bp section
type: SequenceAnnotation
persistent identity: annotation1954616
display id: annotation1954616
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1954616/range1954616/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_I750016/annotation1954616/range1954616/1
type: Range
persistent identity: range1954616
display id: range1954616
version: 1
top level: BBa_I750016
direction: direction/forward
start: 3304
end: 3343
orientation: inline

gvpL g696a
type: SequenceAnnotation
persistent identity: annotation1951698
display id: annotation1951698
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951698/range1951698/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_I750016/annotation1951698/range1951698/1
type: Range
persistent identity: range1951698
display id: range1951698
version: 1
top level: BBa_I750016
direction: direction/forward
start: 3328
end: 3328
orientation: inline

10 repeats of 40bp induced durring mutagenesis
type: SequenceAnnotation
persistent identity: annotation1954598
display id: annotation1954598
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1954598/range1954598/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I750016/annotation1954598/range1954598/1
type: Range
persistent identity: range1954598
display id: range1954598
version: 1
top level: BBa_I750016
direction: direction/forward
start: 3344
end: 3743
orientation: inline

gvpS
type: SequenceAnnotation
persistent identity: annotation1951689
display id: annotation1951689
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951689/range1951689/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I750016/annotation1951689/range1951689/1
type: Range
persistent identity: range1951689
display id: range1951689
version: 1
top level: BBa_I750016
direction: direction/forward
start: 3860
end: 4147
orientation: inline

gvpK
type: SequenceAnnotation
persistent identity: annotation1951690
display id: annotation1951690
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951690/range1951690/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I750016/annotation1951690/range1951690/1
type: Range
persistent identity: range1951690
display id: range1951690
version: 1
top level: BBa_I750016
direction: direction/forward
start: 4101
end: 4385
orientation: inline

gvpJ
type: SequenceAnnotation
persistent identity: annotation1951691
display id: annotation1951691
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951691/range1951691/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I750016/annotation1951691/range1951691/1
type: Range
persistent identity: range1951691
display id: range1951691
version: 1
top level: BBa_I750016
direction: direction/forward
start: 4407
end: 4709
orientation: inline

t->a
type: SequenceAnnotation
persistent identity: annotation1954603
display id: annotation1954603
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1954603/range1954603/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_I750016/annotation1954603/range1954603/1
type: Range
persistent identity: range1954603
display id: range1954603
version: 1
top level: BBa_I750016
direction: direction/forward
start: 4532
end: 4532
orientation: inline

gvpT
type: SequenceAnnotation
persistent identity: annotation1951692
display id: annotation1951692
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951692/range1951692/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I750016/annotation1951692/range1951692/1
type: Range
persistent identity: range1951692
display id: range1951692
version: 1
top level: BBa_I750016
direction: direction/forward
start: 4746
end: 5624
orientation: inline

g->a
type: SequenceAnnotation
persistent identity: annotation1954604
display id: annotation1954604
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1954604/range1954604/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_I750016/annotation1954604/range1954604/1
type: Range
persistent identity: range1954604
display id: range1954604
version: 1
top level: BBa_I750016
direction: direction/forward
start: 5070
end: 5070
orientation: inline

g->a
type: SequenceAnnotation
persistent identity: annotation1954605
display id: annotation1954605
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1954605/range1954605/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_I750016/annotation1954605/range1954605/1
type: Range
persistent identity: range1954605
display id: range1954605
version: 1
top level: BBa_I750016
direction: direction/forward
start: 5133
end: 5133
orientation: inline

gvpU
type: SequenceAnnotation
persistent identity: annotation1951693
display id: annotation1951693
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951693/range1951693/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I750016/annotation1951693/range1951693/1
type: Range
persistent identity: range1951693
display id: range1951693
version: 1
top level: BBa_I750016
direction: direction/forward
start: 5648
end: 6064
orientation: inline

https://synbiohub.org/public/igem/BBa_I20282_sequence/1
type: Sequence
persistent identity: BBa I20282 sequence
display id: BBa_I20282_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagagaaagaggagaaatactagatgtctattcaaaaaagtactaatagttcaagtttagcagaagtcattgaccgtattttagataaaggaattgttattgatgcttttgcaagagtttctgttgtaggaattgaaattttaacgattgaagcgcgagtggttattgccagtgttgatacatggttacgctatgcagaagcagtagggcttcttcgtgacgacgtagaagaaaacggtcttcctgaacgttcaaattcaagtgaagggcagccgcgttttagtatttaatatgaagtggtatggaaccttccttttttgagggtggttccagcttttagatctaactattggaggctactaaaaatggaaattaaaaaaattatgcaagccgtgaacgactttttcggtgaacacgtagctcctcctcataaaattacctcggtggaagctactgaagatgaaggttggagagttattgttgaagtcattgaagaacgagaatatatgaaaaaatacgccaaagatgaaatgctcggaacgtacgagtgctttgtaaataaagaaaaagaagtcatttcattcaaacgactcgacgtcagatatagaagcgccattggcattgaagcataaacgataagatggcaggaggaacgtaaaaatgaccgtcttaacagacaaaaggaaaaaaggcagtggagcttttatacaagatgacgagacaaaagaggttctttcaagagcgctgagctatttaaaatccggctattccattcattttacaggtcctgccggcggaggcaaaacctctttagcgcgagcgcttgctaaaaagagaaagcgtcctgtaatgctgatgcacgggaatcacgagctcaacaacaaagatttaattggcgattttacgggatacacgagcaaaaaagtaatcgaccagtacgttcgttctgtctataaaaaagatgaacaggtgagtgaaaactggcaggatggccgattgcttgaagctgtaaaaaatggctatacgctgatttacgacgaatttactcgttctaagcctgcgacgaataatatctttctatcgatattagaagaaggcgtgctgccgctgtatggagtaaaaatgaccgatccttttgtgcgcgtgcatcccgatttccgcgtcatcttcacaagcaatccagctgagtatgccggcgtatatgatacgcaagatgcgcttctcgacaggttaattaccatgtttattgattataaagacatcgacagagagacagcgattttaacggagaaaacggacgtagaagaagatgaagcgcgcacaattgtaacgctcgtagcaaacgtgcgaaaccgctctggagacgaaaacagcagcggacttagcctgcgggcttcgcttatgatcgctacccttgccacgcagcaagacattcctatcgatggaagtgacgaagattttcaaacgttatgtatcgatattttgcatcatccgcttaccaaatgtttggatgaagaaaatgcaaaaagcaaagccgaaaaaatcattttagaagaatgtaagaatatagacactgaagaaaagtaaaggagcttgaaaacatgagtgaaacaaacgaaacaggtatttatatttttagcgccattcaaacggataaagacgaagaatttggcgccgtggaagtagaaggaacaaaagctgaaacatttttgattcgctacaaagacgcggctatggtagcagctgaagtaccgatgaaaatttatcatcctaatcgccaaaatttattaatgcatcaaaacgcagtagcagcgattatggacaagaacgatacggttattccaatcagctttgggaatgtattcaaatcaaaagaagacgtaaaagttcttttggaaaacctttatccgcagtttgaaaagctgtttccagcgatcaaaggaaaaattgaagtcggtttaaaagtaattgggaaaaaagaatgg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I750016_sequence/1
type: Sequence
persistent identity: BBa I750016 sequence
display id: BBa_I750016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgtctattcaaaaaagtactaatagttcaagtttagcagaagtcattgaccgtattttagataaaggaattgttattgatgcttttgcaagagtttctgttgtaggaattgaaattttaacgattgaagcgcgagtggttattgccagtgttgatacatggttacgctatgcagaagcagtagggcttcttcgtgacgacgtagaagaaaacggtcttcctgaacgttcaaattcaagtgaagggcagccgcgttttagtatttaatatgaagtggtatggaaccttccttttttgagggtggttccagcttttagatctaactattggaggctactaaaaatggaaattaaaaaaattatgcaagccgtgaacgactttttcggtgaacacgtagctcctcctcataaaattacctcggtggaagctactgaagatgaaggttggagagttattgttgaagtcattgaagaacgagaatatatgaaaaaatacgccaaagatgaaatgctcggaacgtacgagtgctttgtaaataaagaaaaagaagtcatttcattcaaacgactcgacgtcagatatagaagcgccattggcattgaagcataaacgataagatggcaggaggaacgtaaaaatgaccgtcttaacagacaaaaggaaaaaaggcagtggagcttttatacaagatgacgagacaaaagaggttctttcaagagcgctgagctatttaaaatccggctattccattcattttacaggtcctgccggcggaggcaaaacctctttagcgcgagcgcttgctaaaaagagaaagcgtcctgtaatgctgatgcacgggaatcacgagctcaacaacaaagatttaattggcgattttacgggatacacgagcaaaaaagtaatcgaccagtacgttcgttctgtctataaaaaagatgaacaggtgagtgaaaactggcaggatggccgattgcttgaagctgtaaaaaatggctatacgctgatttacgacgaatttactcgttctaagcctgcgacgaataatatctttctatcgatattagaagaaggcgtgctgccgctgtatggagtaaaaatgaccgatccttttgtgcgcgtgcatcccgatttccgcgtcatcttcacaagcaatccagctgagtatgccggcgtatatgatacgcaagatgcgcttctcgacaggttaattaccatgtttattgattataaagacatcgacagagagacagcgattttaacggagaaaacggacgtagaagaagatgaagcgcgcacaattgtaacgctcgtagcaaacgtgcgaaaccgctctggagacgaaaacagcagcggacttagcctgcgggcttcgcttatgatcgctacccttgccacgcagcaagacattcctatcgatggaagtgacgaagattttcaaacgttatgtatcgatattttgcatcatccgcttaccaaatgtttggatgaagaaaatgcaaaaagcaaagccgaaaaaatcattttagaagaatgtaagaatatagacactgaagaaaagtaaaggagcttgaaaacatgagtgaaacaaacgaaacaggtatttatatttttagcgccattcaaacggataaagacgaagaatttggcgccgtggaagtagaaggaacaaaagctgaaacatttttgattcgctacaaagacgcggctatggtagcagctgaagtaccgatgaaaatttatcatcctaatcgccaaaatttattaatgcatcaaaacgcagtagcagcgattatggacaagaacgatacggttattccaatcagctttgggaatgtattcaaatcaaaagaagacgtaaaagttcttttggaaaacctttatccgcagtttgaaaagctgtttccagcgatcaaaggaaaaattgaagtcggtttaaaagtaattgggaaaaaagaatggcttgagaaaaaagtaaacga…
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z