##gff-version 3
##sequence-region BBa_I20283 1 1803
BBa_I20283	.	terminator	744	777	.	+	0	ID=BBa_B1002;Name=BBa_B1002
BBa_I20283	.	polyA_site	747	752	.	+	0	ID=annotation1898415;Name=Poly A tail;Parent=BBa_B1002
BBa_I20283	.	polyA_site	768	774	.	+	0	ID=annotation1898414;Name=Poly A tail;Parent=BBa_B1002
BBa_I20283	.	stem_loop	753	768	.	+	0	ID=annotation1898413;Name=stem loop;Parent=BBa_B1002
BBa_I20283	.	engineered_region	744	777	.	+	0	ID=annotation1898412;Name=B1002;Parent=BBa_B1002
BBa_I20283	.	ribosome_entry_site	32	44	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_I20283	.	ribosome_entry_site	32	44	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_I20283	.	engineered_region	32	44	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_I20283	.	ribosome_entry_site	38	41	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_I20283	.	terminator	1770	1803	.	+	0	ID=BBa_B1002;Name=BBa_B1002
BBa_I20283	.	polyA_site	1773	1778	.	+	0	ID=annotation1898415;Name=Poly A tail;Parent=BBa_B1002
BBa_I20283	.	polyA_site	1794	1800	.	+	0	ID=annotation1898414;Name=Poly A tail;Parent=BBa_B1002
BBa_I20283	.	stem_loop	1779	1794	.	+	0	ID=annotation1898413;Name=stem loop;Parent=BBa_B1002
BBa_I20283	.	engineered_region	1770	1803	.	+	0	ID=annotation1898412;Name=B1002;Parent=BBa_B1002
BBa_I20283	.	promoter	786	839	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I20283	.	non_covalent_binding_site	786	804	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I20283	.	engineered_region	786	839	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I20283	.	promoter	805	810	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I20283	.	promoter	828	833	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I20283	.	non_covalent_binding_site	811	829	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I20283	.	ribosome_entry_site	1749	1761	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_I20283	.	ribosome_entry_site	1749	1761	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_I20283	.	engineered_region	1749	1761	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_I20283	.	ribosome_entry_site	1755	1758	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_I20283	.	CDS	867	1641	.	+	0	ID=BBa_C0051;Name=BBa_C0051
BBa_I20283	.	tag	1578	1610	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_I20283	.	engineered_tag	1617	1641	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_I20283	.	CDS	870	1577	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_I20283	.	terminator	1650	1683	.	+	0	ID=BBa_B1002;Name=BBa_B1002
BBa_I20283	.	polyA_site	1653	1658	.	+	0	ID=annotation1898415;Name=Poly A tail;Parent=BBa_B1002
BBa_I20283	.	polyA_site	1674	1680	.	+	0	ID=annotation1898414;Name=Poly A tail;Parent=BBa_B1002
BBa_I20283	.	stem_loop	1659	1674	.	+	0	ID=annotation1898413;Name=stem loop;Parent=BBa_B1002
BBa_I20283	.	engineered_region	1650	1683	.	+	0	ID=annotation1898412;Name=B1002;Parent=BBa_B1002
BBa_I20283	.	promoter	1	23	.	+	0	ID=BBa_I719005;Name=BBa_I719005
BBa_I20283	.	ribosome_entry_site	848	860	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_I20283	.	ribosome_entry_site	848	860	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_I20283	.	engineered_region	848	860	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_I20283	.	ribosome_entry_site	854	857	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_I20283	.	CDS	51	735	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_I20283	.	engineered_tag	711	735	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_I20283	.	CDS	54	670	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_I20283	.	tag	671	704	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_I20283	.	promoter	1692	1740	.	+	0	ID=BBa_R0051;Name=BBa_R0051
BBa_I20283	.	promoter	1706	1711	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_I20283	.	non_covalent_binding_site	1716	1732	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_I20283	.	non_covalent_binding_site	1692	1708	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_I20283	.	engineered_region	1692	1740	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_I20283	.	promoter	1729	1734	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
>BBa_I20283
taatacgactcactatagggagatactagagtcacacaggaaagtactagatgtccagattagataaaagtaaagtgat
taacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgta
gagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggc
accatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgc
tttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaat
caattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactt
taggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgcc
attattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcata
tgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataac
actgatagtgctagtgtagatcactactagagcgcaaaaaaccccgcttcggcggggttttttcgctactagagtccct
atcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacacaggaaagtactagatg
agcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaa
atgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatgg
catcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttca
atcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttt
tttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcac
aaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagcca
agctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttg
ggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtaccc
aatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgct
gcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagcgcaaaaaac
cccgcttcggcggggttttttcgctactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggtt
gctactagagtcacacaggaaagtactagagcgcaaaaaaccccgcttcggcggggttttttcgc