BBa_I20284 1 Bxb1 Bacteriophage Bxb1 Integrase-Freiburg standard 2009-07-09T11:00:00Z 2015-08-31T04:07:40Z Source: http://www.uniprot.org/uniprot/Q9B086 References: [1] Genome organization and characterization of mycobacteriophage Bxb1., mediavilla et al., Mol microbiol, 2000. PMID 1123671. [2] Mycobacteriophage Bxb1 integrates into the Mycobacterium smegmatis groEL1 gene. Kim AI, Ghosh P, Aaron MA, Bibb LA, Jain S, Hatfull GF. Mol Microbiol. 2003 [3] The orientation of mycobacteriophage Bxb1 integration is solely dependent on the central dinucleotide of attP and attB. Ghosh P, Kim AI, Hatfull GF. Mol Cell. 2003 PMID:14636570 [4] Control of phage Bxb1 excision by a novel recombination directionality factor., Ghosh P. et al, PLoS Biol., 2006. PMID: 16719562. [5] Phage Bxb1 integrase mediates highly efficient site-specific recombination in mammalian cells., Russel JP et al., Biotechniques, 2006. PMID:16629393 [6] A diversity of serine phage integrases mediate site-specific recombination in mammalian cells. Keravala A, Groth AC, Jarrahian S, Thyagarajan B, Hoyt JJ, Kirby PJ, Calos MP. Mol Genet Genomics, 2006 PMID: 16699779 Bxb1 integrase is a recombinase enzyme from the serine recombinase family. Bxb1 naturally catalyze integration of the phage genome into the GroEL1 gene of M.segmentis, which contains the attB site [2]. Minimal attB, attP,[2],[3] attL and attR [4] have been characterized. Excisionase has been identified, is relatively big (28kDa) for a RDF, and is efficient both in vivo and in vitro [4]. In vitro recombination is very efficient ranging a 100% efficiency in 1hour for integration [2] and for excision [4]. Interestingly the excisionase subunit does not bind DNA and acts stoechiometrically on Bxb1 integrase [4]. The enzyme does not require supercoiled DNA, and can also works with linear products [2]. The enzyme also works in human, rat, and mouse cells [5, 6]. When recombination sites are placed in the same DNA molecule, recombination outcome is inversion of the sequence they surround. false false _11_ 0 3868 11 Not in stock false Sequences cleaned up for restriction sites according to Freiburg standard (25) false Jerome Bonnet BBa_I20284_sequence 1 atggccggcagagccctggtagtcatccgcctgtcccgcgtcaccgatgctacgacttcaccggagcgtcagctggagtcttgccagcagctctgcgcccagcgcggctgggacgtcgtcggggtagcggaggatctggacgtctccggggcggtcgatccgttcgaccggaagcgcagaccgaacctggcccggtggctagcgttcgaggagcaaccgtttgacgtgatcgtggcgtaccgggtagatcggttgacccgatcgatccggcatcttcagcagctggtccactgggccgaggaccacaagaagctggtcgtctccgcgaccgaagcgcacttcgatacgacgacgccgtttgcggcggtcgtcatcgcgcttatgggaacggtggcgcagatggaattagaagcgatcaaagagcggaaccgttcggctgcgcatttcaatatccgcgccgggaaataccgaggatccctgccgccgtggggatacctgcctacgcgcgtggacggggagtggcggctggtgccggaccctgtgcagcgagagcgcatcctcgaggtgtatcaccgcgtcgtcgacaaccacgagccgctgcatctggtggcccacgacctgaaccggcgtggtgtcctgtcgccgaaggactacttcgcgcagctgcaaggccgcgagccgcagggccgggagtggtcggctaccgcgctgaagcgatcgatgatctccgaggcgatgctcgggtacgcgactctgaacggtaagaccgtccgagacgacgacggagccccgctggtgcgggctgagccgatcctgacccgtgagcagctggaggcgctgcgcgccgagctcgtgaagacctcccgggcgaagcccgcggtgtctaccccgtcgctgctgctgcgggtgttgttctgcgcggtgtgcggggagcccgcgtacaagttcgccgggggaggacgtaagcacccgcgctaccgctgccgctcgatggggttcccgaagcactgcgggaacggcacggtggcgatggccgagtgggacgcgttctgcgaggagcaggtactggatctgctcggggacgcggagcgtctggagaaagtctgggtagcgggctcggactccgcggtcgaactcgcggaggtgaacgcggagctggtggacctgacgtcgctgatcggctccccggcctaccgggcgggctctccgcagcgagaagcactggatgcccgtattgcggcgctggccgcgcggcaagaggagctggagggcctggaggctcgcccgtctggctgggagtggcgcgagaccgggcagcggttcggggactggtggcgggagcaggacaccgcggcaaagaacacctggcttcggtcgatgaacgttcggctgacgttcgacgtccgcggcgggctgactcgcacgatcgacttcggggatcttcaggagtacgagcagcatctcaggctcggcagcgtggtcgaacggctacacaccgggatgtcgaccggttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z