BBa_I20285 1 BBa_I20285 Bxb1 Excisionase-Freiburg standard 2009-07-10T11:00:00Z 2015-08-31T04:07:40Z http://www.uniprot.org/uniprot/Q9B074 This protein is a recombination directionality factor (RDF). Association with Bxb1 integrase, and triggers recombination between AttL and attR and excision of the phage genome. Bxb1 Excisionase is relatively big (28kDa) for a RDF, and is efficient both in vivo and in vitro [4]. In vitro recombination is very efficient ranging a 100% efficiency in 1hour for integration [2] and for excision [4]. Interestingly the excisionase subunit does not bind DNA and acts stoechiometrically on Bxb1 integrase [4]. Reverse inversion reaction when attL and attR in same DNA molecule. No known catalytic activity per se. false false _11_ 0 3868 11 Not in stock false sequence cleaned for restriction sites according to Freiburg standard. false Jerome Bonnet annotation2011203 1 NgoMIV range2011203 1 4 9 annotation2011202 1 Start range2011202 1 1 3 annotation2011204 1 AgeI range2011204 1 774 779 BBa_I20285_sequence 1 atggccggcactcagcgtatcgtctttctacccgatactcagttgcctttcgaggcgcgcaaagagatgcaagcggtcatccgcttcatcggggatgtccagccgtacggcgtggtacatatcggtgacgtcctagacctgccgcagccctcgcgctggaaccggggaaccaagggcgagttcgagggctcggtgtaccgcgacgcggactacgccaagaagaacctgatggagccgctgcgcaaggtctacgacggctggatcgggatgcacgagggcaaccacgatctgcgagcccgcgagtacctggccaagaacgcaccggccctggagggtacgcacgctttcgacatcgacgtgctgctcgacttcgacgggttcggtgtggagctgctgcctgacttctacgacatcgctccgggctggatctccactcacgggcacatgggcaagatgacgctatcccagatcgccggatcgacagcgctcaacggtgccaagaagttcggcaagtccgtggtctgcggccacacgcaccggcaggctgtcgtctcgcactcgttcgggtacggcggctcggtgcgcaagaccgtcaccggcatggaagtcgggcacctgatggacatgaagaaggccaactatctaaagggcggagccgggaactggcagatgggcttcgggatgctcacggtcgacggcaagcatgtcaaggctgagatcgtcccgatcctgggaggcaagttcaccgttgacggccaggtctgggaagtcaccggttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z