LOCUS BBa_I2029 3876 bp DNA linear 22-Dec-2024 DEFINITION RhlI/RhlR Signal Test (I2027.I2028) ACCESSION BBa_I2029 VERSION BBa_I2029.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1223..1226 /label=conserved RBS 1219..1230 /label=BBa_B0034 CDS 1237..1839 /label=rhlL misc_feature 1840..1872 /label=LVA misc_feature 1879..1903 /label=Help:Barcodes CDS 1237..1878 /label=BBa_C0070 stem_loop 1923..1966 /label=stem_loop misc_feature 1912..1991 /label=BBa_B0010 terminator 1912..1991 /label=BBa_B0010 stem_loop 2007..2026 /label=T7 TE misc_feature 2033..2033 /label=stop polyA_site 2027..2040 /label=polya misc_feature 2000..2040 /label=BBa_B0012 terminator 2000..2040 /label=BBa_B0012 promoter 2083..2088 /label=-10 promoter 2063..2068 /label=-35 promoter 2049..2097 /label=BBa_R1075 misc_feature 2110..2113 /label=conserved RBS 2106..2117 /label=BBa_B0034 CDS 2124..2846 /label=rhlR misc_feature 2847..2879 /label=LVA misc_feature 2886..2910 /label=Help:Barcodes CDS 2124..2885 /label=BBa_C0071 stem_loop 2930..2973 /label=stem_loop misc_feature 2919..2998 /label=BBa_B0010 terminator 2919..2998 /label=BBa_B0010 stem_loop 3014..3033 /label=T7 TE misc_feature 3040..3040 /label=stop polyA_site 3034..3047 /label=polya misc_feature 3007..3047 /label=BBa_B0012 terminator 3007..3047 /label=BBa_B0012 misc_feature 3056..3075 /label=RhlR promoter 3074..3079 /label=-35 promoter 3097..3102 /label=-10 misc_feature 3108..3108 /label=start promoter 3056..3108 /label=BBa_R0071 CDS 3115..3876 /label=CFP (LVA) misc_feature 3833..3870 /label=SsrA misc_feature 3871..3876 /label=2 misc_feature 3183..3183 /label=A misc_feature 3115..3876 /label=BBa_E0022 misc_feature 3115..3876 /label=BBa_E0022 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatga tcgaactgct gtccgaatcc 1261 ctggaaggtc tgtccgctgc tatgatcgct gaactgggtc gttaccgtca ccaggttttc 1321 atcgaaaaac tgggttggga cgttgtttcc acctcccgtg ttcgtgacca ggagttcgac 1381 cagttcgacc acccgcagac ccgttacatc gttgctatgt cccgtcaggg tatctgcggt 1441 tgcgctcgtc tgctgccgac caccgacgct tacctgctga aagacgtttt cgcttacctg 1501 tgctccgaaa ccccgccgtc cgacccgtcc gtttgggaac tgtcccgtta cgctgcttcc 1561 gctgctgacg acccgcagct ggctatgaaa atcttctggt cctccctcca gtgcgcttgg 1621 tacctgggtg cttcctccgt tgttgctgtt accaccaccg ctatggaacg ttacttcgtt 1681 cgtaacggtg ttatcctcca gcgtctgggt ccgccgcaga aagttaaagg tgaaaccctg 1741 gttgctatct ccttcccggc ttaccaggaa cgtggtctgg aaatgctgct gcgttaccac 1801 ccggaatggc tccagggtgt tccgctgtcc atggctgttg ctgcaaacga cgaaaactac 1861 gctttagtag cttaataact ctgatagtgc tagtgtagat ctctactaga gccaggcatc 1921 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 1981 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata 2041 tactagagtt aaatttcctc ttttcaggcc ggaataactc cctataatgc gccaccatac 2101 tagagaaaga ggagaaatac tagatgagga atgacggagg ctttttgctg tggtgggacg 2161 gtttgcgtag cgagatgcag ccgatccacg acagccaggg cgtgttcgcc gtcctggaaa 2221 aggaagtgcg gcgcctgggc ttcgattact acgcctatgg cgtgcgccac acgattccct 2281 tcacccggcc gaagaccgag gtccatggca cctatcccaa ggcctggctg gagcgatacc 2341 agatgcagaa ctacggggcc gtggatccgg cgatcctcaa cggcctgcgc tcctcggaaa 2401 tggtggtctg gagcgacagc ctgttcgacc agagccggat gctctggaac gaggctcgcg 2461 attggggcct ctgtgtcggc gcgaccttgc cgatccgcgc gccgaacaat ttgctcagcg 2521 tgctttccgt ggcgcgcgac cagcagaaca tctccagctt cgagcgcgag gaaatccgcc 2581 tgcggctgcg ttgcatgatc gagttgctga cccagaagct gaccgacctg gagcatccga 2641 tgctgatgtc caacccggtc tgcctgagcc atcgcgaacg cgagatcctg caatggaccg 2701 ccgacggcaa gagttccggg gaaatcgcca tcatcctgag catctccgag agcacggtga 2761 acttccacca caagaacatc cagaagaagt tcgacgcgcc gaacaagacg ctggctgccg 2821 cctacgccgc ggcgctgggt ctcatcgctg caaacgacga aaactacgct ttagtagctt 2881 aataacactg atagtgctag tgtagatcac tactagagcc aggcatcaaa taaaacgaaa 2941 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta 3001 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac tagagtcctg 3061 tgaaatctgg cagttaccgt tagctttcga attggctaaa aagtgttcta ctagatggtg 3121 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac 3181 gtgaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag 3241 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg 3301 accaccctga cctggggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac 3361 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag 3421 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac 3481 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg 3541 gagtacaact acatcagcca caacgtctat atcaccgccg acaagcagaa gaacggcatc 3601 aaggccaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac 3661 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg 3721 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg 3781 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gaggcctgct 3841 gcaaacgacg aaaactacgc tttagtagct taataa //