LOCUS       BBa_I20294              1005 bp    DNA     linear       16-May-2024
DEFINITION  T7 RNAP dependent expression of GFP-LacZalpha fusion
ACCESSION   BBa_I20294
VERSION     BBa_I20294.1  
FEATURES             Location/Qualifiers
     promoter        1..23
                     /label=BBa_I719005
     misc_feature    38..41
                     /label=conserved
     RBS             32..45
                     /label=BBa_B0031
     CDS             52..231
                     /label=lacZa
     misc_feature    232..288
                     /label=linker
     CDS             289..1005
                     /label=GFP
     misc_feature    52..1005
                     /label=BBa_E0051
ORIGIN
        1 taatacgact cactataggg agatactaga gtcacacagg aaacctacta gatgaccatg
       61 attacggatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
      121 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
      181 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg cgcgggcggc
      241 agcgaaggcg gcggcagcga acatcatcat catcatcatg gcagcgaacg taaaggagaa
      301 gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac
      361 aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa
      421 tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac taccctgacc
      481 tatggtgttc aatgctttgc gagataccca gatcatatga aacagcatga ctttttcaag
      541 agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga tgacgggaac
      601 tacaagacac gtgctgaagt caagtttgaa ggtgataccc ttgttaatag aatcgagtta
      661 aaaggtattg attttaaaga agatggaaac attcttggac acaaattgga atacaactat
      721 aactcacaca atgtatacat catggcagac aaacaaaaga atggaatcaa agttaacttc
      781 aaaattagac acaacattga agatggaagc gttcaactag cagaccatta tcaacaaaat
      841 actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc cacacaatct
      901 gccctttcga aagatcccaa cgaaaagaga gaccacatgg tccttcttga gtttgtaaca
      961 gctgctggga ttacacatgg catggatgaa ctatacaaat aataa
//