LOCUS       BBa_I20295              1004 bp    DNA     linear       14-May-2024
DEFINITION  T7 RNAP dependent expression of GFP-LacZalpha fusion
ACCESSION   BBa_I20295
VERSION     BBa_I20295.1  
FEATURES             Location/Qualifiers
     promoter        1..23
                     /label=BBa_I719005
     RBS             32..44
                     /label=RBS-3\Weak
     RBS             38..41
                     /label=RBS
     misc_feature    32..44
                     /label=BBa_B0032
     RBS             32..44
                     /label=BBa_B0032
     CDS             51..230
                     /label=lacZa
     misc_feature    231..287
                     /label=linker
     CDS             288..1004
                     /label=GFP
     misc_feature    51..1004
                     /label=BBa_E0051
ORIGIN
        1 taatacgact cactataggg agatactaga gtcacacagg aaagtactag atgaccatga
       61 ttacggattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
      121 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
      181 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc gcgggcggca
      241 gcgaaggcgg cggcagcgaa catcatcatc atcatcatgg cagcgaacgt aaaggagaag
      301 aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca
      361 aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat
      421 ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact accctgacct
      481 atggtgttca atgctttgcg agatacccag atcatatgaa acagcatgac tttttcaaga
      541 gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat gacgggaact
      601 acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa
      661 aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa tacaactata
      721 actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gttaacttca
      781 aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat caacaaaata
      841 ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc acacaatctg
      901 ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag
      961 ctgctgggat tacacatggc atggatgaac tatacaaata ataa
//