LOCUS       BBa_I20296              1003 bp    DNA     linear       19-Dec-2024
DEFINITION  T7 RNAP dependent expression of GFP-LacZalpha fusion
ACCESSION   BBa_I20296
VERSION     BBa_I20296.1  
FEATURES             Location/Qualifiers
     promoter        1..23
                     /label=BBa_I719005
     misc_feature    36..39
                     /label=conserved
     RBS             32..43
                     /label=BBa_B0034
     CDS             50..229
                     /label=lacZa
     misc_feature    230..286
                     /label=linker
     CDS             287..1003
                     /label=GFP
     misc_feature    50..1003
                     /label=BBa_E0051
ORIGIN
        1 taatacgact cactataggg agatactaga gaaagaggag aaatactaga tgaccatgat
       61 tacggattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
      121 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
      181 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcg cgggcggcag
      241 cgaaggcggc ggcagcgaac atcatcatca tcatcatggc agcgaacgta aaggagaaga
      301 acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa
      361 attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt
      421 tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta ccctgaccta
      481 tggtgttcaa tgctttgcga gatacccaga tcatatgaaa cagcatgact ttttcaagag
      541 tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg acgggaacta
      601 caagacacgt gctgaagtca agtttgaagg tgataccctt gttaatagaa tcgagttaaa
      661 aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat acaactataa
      721 ctcacacaat gtatacatca tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa
      781 aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc aacaaaatac
      841 tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca cacaatctgc
      901 cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt ttgtaacagc
      961 tgctgggatt acacatggca tggatgaact atacaaataa taa
//