BBa_I20299 1 BBa_I20299 RBS from T7 gene 4, optimal aligned spacing 2010-06-20T11:00:00Z 2015-08-31T04:07:40Z Derived from the RBS of phage T7 gene 4 RBS derived from phage T7 gene 4. Similar to I20298, but with the last (3') nucleotides removed. This produces an optimal aligned spacing of 5 nucleotides between the Shine-Dalgarno (SD) sequence and the ATG of the following CDS, when the RBS is placed immediately before the ATG i.e. without Biobrick "scar". For information on "optimal aligned spacing", see Chen et al., (1994) NAR, 22(23):4953-7 [PMID 7528374] false false _11_ 0 1950 11 Not in stock false See "long description" false Francois St-Pierre BBa_I20299_sequence 1 tacgaggaggatgaag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z