##gff-version 3
##sequence-region BBa_I2030 1 11458
BBa_I2030	.	ribosome_entry_site	7027	7038	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I2030	.	conserved_region	7031	7034	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I2030	.	ribosome_entry_site	8022	8033	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I2030	.	conserved_region	8026	8029	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I2030	.	CDS	4590	5256	.	+	0	ID=BBa_C0078;Name=BBa_C0078
BBa_I2030	.	tag	5193	5225	.	+	0	ID=annotation306608;Name=LVA;Parent=BBa_C0078
BBa_I2030	.	CDS	4590	5192	.	+	0	ID=annotation305970;Name=LasI;Parent=BBa_C0078
BBa_I2030	.	engineered_tag	5232	5256	.	+	0	ID=annotation2214003;Name=Help:Barcodes;Parent=BBa_C0078
BBa_I2030	.	ribosome_entry_site	9186	9197	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I2030	.	conserved_region	9190	9193	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I2030	.	terminator	7720	7848	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_I2030	.	terminator	7808	7848	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I2030	.	stem_loop	7815	7834	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I2030	.	stop_codon	7841	7841	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I2030	.	polyA_site	7835	7848	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I2030	.	engineered_region	7808	7848	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I2030	.	terminator	7720	7799	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I2030	.	stem_loop	7731	7774	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I2030	.	engineered_region	7720	7799	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I2030	.	ribosome_entry_site	3861	3872	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I2030	.	conserved_region	3865	3868	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I2030	.	engineered_region	2171	3644	.	+	0	ID=BBa_Q04120;Name=BBa_Q04120
BBa_I2030	.	promoter	3445	3644	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_Q04120
BBa_I2030	.	promoter	3581	3586	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_I2030	.	CDS	3445	3532	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_I2030	.	engineered_region	3445	3644	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_I2030	.	promoter	3605	3610	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_I2030	.	non_covalent_binding_site	3610	3644	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_I2030	.	start_codon	3617	3617	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_I2030	.	non_covalent_binding_site	3533	3570	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_I2030	.	ribosome_entry_site	2171	2182	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04120
BBa_I2030	.	conserved_region	2175	2178	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I2030	.	CDS	2189	3274	.	+	0	ID=BBa_C0013;Name=BBa_C0013;Parent=BBa_Q04120
BBa_I2030	.	engineered_tag	3275	3299	.	+	0	ID=annotation2214005;Name=Help:Barcodes;Parent=BBa_C0013
BBa_I2030	.	terminator	3396	3436	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q04120
BBa_I2030	.	stem_loop	3403	3422	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I2030	.	stop_codon	3429	3429	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I2030	.	polyA_site	3423	3436	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I2030	.	engineered_region	3396	3436	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I2030	.	terminator	3308	3387	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q04120
BBa_I2030	.	stem_loop	3319	3362	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I2030	.	engineered_region	3308	3387	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I2030	.	CDS	8040	8979	.	+	0	ID=BBa_C0080;Name=BBa_C0080
BBa_I2030	.	tag	8916	8948	.	+	0	ID=annotation308196;Name=LVA;Parent=BBa_C0080
BBa_I2030	.	CDS	8040	8915	.	+	0	ID=annotation308191;Name=araC;Parent=BBa_C0080
BBa_I2030	.	engineered_tag	8955	8979	.	+	0	ID=annotation2214004;Name=Help:Barcodes;Parent=BBa_C0080
BBa_I2030	.	promoter	9125	9177	.	+	0	ID=BBa_R0071;Name=BBa_R0071
BBa_I2030	.	non_covalent_binding_site	9125	9144	.	+	0	ID=annotation297102;Name=RhlR;Parent=BBa_R0071
BBa_I2030	.	promoter	9166	9171	.	+	0	ID=annotation297104;Name=-10;Parent=BBa_R0071
BBa_I2030	.	promoter	9143	9148	.	+	0	ID=annotation297103;Name=-35;Parent=BBa_R0071
BBa_I2030	.	start_codon	9177	9177	.	+	0	ID=annotation297105;Name=start;Parent=BBa_R0071
BBa_I2030	.	engineered_region	1	1252	.	+	0	ID=BBa_Q04800;Name=BBa_Q04800
BBa_I2030	.	terminator	1055	1095	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q04800
BBa_I2030	.	stem_loop	1062	1081	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I2030	.	stop_codon	1088	1088	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I2030	.	polyA_site	1082	1095	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I2030	.	engineered_region	1055	1095	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I2030	.	CDS	19	933	.	+	0	ID=BBa_C0080;Name=BBa_C0080;Parent=BBa_Q04800
BBa_I2030	.	tag	895	927	.	+	0	ID=annotation308196;Name=LVA;Parent=BBa_C0080
BBa_I2030	.	CDS	19	894	.	+	0	ID=annotation308191;Name=araC;Parent=BBa_C0080
BBa_I2030	.	engineered_tag	934	958	.	+	0	ID=annotation2214004;Name=Help:Barcodes;Parent=BBa_C0080
BBa_I2030	.	terminator	967	1046	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q04800
BBa_I2030	.	stem_loop	978	1021	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I2030	.	engineered_region	967	1046	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I2030	.	promoter	1104	1252	.	+	0	ID=BBa_R0080;Name=BBa_R0080;Parent=BBa_Q04800
BBa_I2030	.	operator	1109	1147	.	+	0	ID=annotation301457;Name=araO1;Parent=BBa_R0080
BBa_I2030	.	non_covalent_binding_site	1107	1132	.	+	0	ID=annotation301456;Name=c-amp2;Parent=BBa_R0080
BBa_I2030	.	promoter	1216	1221	.	+	0	ID=annotation308601;Name=-35;Parent=BBa_R0080
BBa_I2030	.	non_covalent_binding_site	1146	1175	.	+	0	ID=annotation301458;Name=c-amp1;Parent=BBa_R0080
BBa_I2030	.	non_covalent_binding_site	1176	1204	.	+	0	ID=annotation301462;Name=ara1 and ara2;Parent=BBa_R0080
BBa_I2030	.	promoter	1239	1244	.	+	0	ID=annotation308602;Name=-10;Parent=BBa_R0080
BBa_I2030	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04800
BBa_I2030	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I2030	.	terminator	10323	10451	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_I2030	.	terminator	10411	10451	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I2030	.	stem_loop	10418	10437	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I2030	.	stop_codon	10444	10444	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I2030	.	polyA_site	10438	10451	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I2030	.	engineered_region	10411	10451	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I2030	.	terminator	10323	10402	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I2030	.	stem_loop	10334	10377	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I2030	.	engineered_region	10323	10402	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I2030	.	engineered_region	5402	6337	.	+	0	ID=BBa_I2010;Name=BBa_I2010
BBa_I2030	.	terminator	6209	6288	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I2010
BBa_I2030	.	stem_loop	6220	6263	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I2030	.	engineered_region	6209	6288	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I2030	.	terminator	6297	6337	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I2010
BBa_I2030	.	stem_loop	6304	6323	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I2030	.	stop_codon	6330	6330	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I2030	.	polyA_site	6324	6337	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I2030	.	engineered_region	6297	6337	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I2030	.	CDS	5420	6175	.	+	0	ID=BBa_C0079;Name=BBa_C0079;Parent=BBa_I2010
BBa_I2030	.	CDS	5420	6136	.	+	0	ID=annotation307935;Name=lasR;Parent=BBa_C0079
BBa_I2030	.	tag	6137	6169	.	+	0	ID=annotation307948;Name=LVA;Parent=BBa_C0079
BBa_I2030	.	engineered_tag	6176	6200	.	+	0	ID=annotation2213999;Name=Help:Barcodes;Parent=BBa_C0079
BBa_I2030	.	sequence_alteration	5899	5899	.	+	0	ID=annotation308006;Name=G;Parent=BBa_C0079
BBa_I2030	.	ribosome_entry_site	5402	5413	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I2010
BBa_I2030	.	conserved_region	5406	5409	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I2030	.	terminator	8988	9116	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_I2030	.	terminator	9076	9116	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I2030	.	stem_loop	9083	9102	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I2030	.	stop_codon	9109	9109	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I2030	.	polyA_site	9103	9116	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I2030	.	engineered_region	9076	9116	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I2030	.	terminator	8988	9067	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I2030	.	stem_loop	8999	9042	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I2030	.	engineered_region	8988	9067	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I2030	.	ribosome_entry_site	4572	4583	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I2030	.	conserved_region	4576	4579	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I2030	.	ribosome_entry_site	10517	10528	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I2030	.	conserved_region	10521	10524	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I2030	.	promoter	10460	10508	.	+	0	ID=BBa_R1075;Name=BBa_R1075
BBa_I2030	.	promoter	10474	10479	.	+	0	ID=annotation309024;Name=-35;Parent=BBa_R1075
BBa_I2030	.	promoter	10494	10499	.	+	0	ID=annotation309020;Name=-10;Parent=BBa_R1075
BBa_I2030	.	promoter	6862	7018	.	+	0	ID=BBa_R0079;Name=BBa_R0079
BBa_I2030	.	non_covalent_binding_site	6907	6924	.	+	0	ID=annotation300985;Name=OP2;Parent=BBa_R0079
BBa_I2030	.	promoter	6978	6983	.	+	0	ID=annotation318495;Name=-35;Parent=BBa_R0079
BBa_I2030	.	promoter	7001	7006	.	+	0	ID=annotation318496;Name=-10;Parent=BBa_R0079
BBa_I2030	.	non_covalent_binding_site	6967	6986	.	+	0	ID=annotation300992;Name=OP1;Parent=BBa_R0079
BBa_I2030	.	CDS	9204	10314	.	+	0	ID=BBa_C0013;Name=BBa_C0013
BBa_I2030	.	engineered_tag	10290	10314	.	+	0	ID=annotation2214005;Name=Help:Barcodes;Parent=BBa_C0013
BBa_I2030	.	promoter	7857	8013	.	+	0	ID=BBa_R0079;Name=BBa_R0079
BBa_I2030	.	non_covalent_binding_site	7902	7919	.	+	0	ID=annotation300985;Name=OP2;Parent=BBa_R0079
BBa_I2030	.	promoter	7973	7978	.	+	0	ID=annotation318495;Name=-35;Parent=BBa_R0079
BBa_I2030	.	promoter	7996	8001	.	+	0	ID=annotation318496;Name=-10;Parent=BBa_R0079
BBa_I2030	.	non_covalent_binding_site	7962	7981	.	+	0	ID=annotation300992;Name=OP1;Parent=BBa_R0079
BBa_I2030	.	CDS	10535	11321	.	+	0	ID=BBa_C0071;Name=BBa_C0071
BBa_I2030	.	tag	11258	11290	.	+	0	ID=annotation306523;Name=LVA;Parent=BBa_C0071
BBa_I2030	.	engineered_tag	11297	11321	.	+	0	ID=annotation2213995;Name=Help:Barcodes;Parent=BBa_C0071
BBa_I2030	.	CDS	10535	11257	.	+	0	ID=annotation301192;Name=rhlR;Parent=BBa_C0071
BBa_I2030	.	terminator	5265	5393	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_I2030	.	terminator	5353	5393	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I2030	.	stem_loop	5360	5379	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I2030	.	stop_codon	5386	5386	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I2030	.	polyA_site	5380	5393	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I2030	.	engineered_region	5353	5393	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I2030	.	terminator	5265	5344	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I2030	.	stem_loop	5276	5319	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I2030	.	engineered_region	5265	5344	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I2030	.	promoter	3653	3852	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_I2030	.	promoter	3789	3794	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_I2030	.	CDS	3653	3740	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_I2030	.	engineered_region	3653	3852	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_I2030	.	promoter	3813	3818	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_I2030	.	non_covalent_binding_site	3818	3852	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_I2030	.	start_codon	3825	3825	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_I2030	.	non_covalent_binding_site	3741	3778	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_I2030	.	terminator	11330	11458	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_I2030	.	terminator	11418	11458	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I2030	.	stem_loop	11425	11444	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I2030	.	stop_codon	11451	11451	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I2030	.	polyA_site	11445	11458	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I2030	.	engineered_region	11418	11458	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I2030	.	terminator	11330	11409	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I2030	.	stem_loop	11341	11384	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I2030	.	engineered_region	11330	11409	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I2030	.	engineered_region	6346	6853	.	+	0	ID=BBa_I2012;Name=BBa_I2012
BBa_I2030	.	terminator	6725	6853	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_I2012
BBa_I2030	.	terminator	6813	6853	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I2030	.	stem_loop	6820	6839	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I2030	.	stop_codon	6846	6846	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I2030	.	polyA_site	6840	6853	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I2030	.	engineered_region	6813	6853	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I2030	.	terminator	6725	6804	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I2030	.	stem_loop	6736	6779	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I2030	.	engineered_region	6725	6804	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I2030	.	ribosome_entry_site	6346	6357	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I2012
BBa_I2030	.	conserved_region	6350	6353	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I2030	.	engineered_region	6364	6716	.	+	0	ID=BBa_E0033;Name=BBa_E0033;Parent=BBa_I2012
BBa_I2030	.	promoter	6536	6554	.	+	0	ID=annotation1938940;Name=T7 promoter;Parent=BBa_E0033
BBa_I2030	.	sequence_feature	6562	6711	.	+	0	ID=annotation1938941;Name=lacZ gene fragment;Parent=BBa_E0033
BBa_I2030	.	sequence_alteration	6450	6450	.	+	0	ID=annotation308381;Name=A;Parent=BBa_E0033
BBa_I2030	.	sequence_alteration	6441	6441	.	+	0	ID=annotation308387;Name=C;Parent=BBa_E0033
BBa_I2030	.	sequence_alteration	6444	6444	.	+	0	ID=annotation308375;Name=T;Parent=BBa_E0033
BBa_I2030	.	CDS	6364	6711	.	+	0	ID=annotation308320;Name=lacZ alpha;Parent=BBa_E0033
BBa_I2030	.	promoter	6389	6398	.	+	0	ID=annotation1938939;Name=T3 promoter;Parent=BBa_E0033
BBa_I2030	.	sequence_alteration	6471	6471	.	+	0	ID=annotation308383;Name=C;Parent=BBa_E0033
BBa_I2030	.	sequence_alteration	6474	6474	.	+	0	ID=annotation308388;Name=G;Parent=BBa_E0033
BBa_I2030	.	sequence_feature	6364	6378	.	+	0	ID=annotation1938942;Name=lacZ gene fragment;Parent=BBa_E0033
BBa_I2030	.	CDS	3879	4563	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_I2030	.	tag	4499	4532	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_I2030	.	engineered_tag	4539	4563	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_I2030	.	CDS	3882	4498	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_I2030	.	engineered_region	1261	2162	.	+	0	ID=BBa_Q04400;Name=BBa_Q04400
BBa_I2030	.	terminator	1972	2051	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q04400
BBa_I2030	.	stem_loop	1983	2026	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I2030	.	engineered_region	1972	2051	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I2030	.	CDS	1279	1938	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_Q04400
BBa_I2030	.	tag	1899	1932	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_I2030	.	engineered_tag	1939	1963	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_I2030	.	CDS	1282	1898	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_I2030	.	promoter	2109	2162	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_Q04400
BBa_I2030	.	engineered_region	2109	2162	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I2030	.	promoter	2128	2133	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I2030	.	non_covalent_binding_site	2109	2127	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I2030	.	promoter	2151	2156	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I2030	.	non_covalent_binding_site	2134	2152	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I2030	.	terminator	2060	2100	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q04400
BBa_I2030	.	stem_loop	2067	2086	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I2030	.	stop_codon	2093	2093	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I2030	.	polyA_site	2087	2100	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I2030	.	engineered_region	2060	2100	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I2030	.	ribosome_entry_site	1261	1272	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04400
BBa_I2030	.	conserved_region	1265	1268	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I2030	.	CDS	7045	7711	.	+	0	ID=BBa_C0070;Name=BBa_C0070
BBa_I2030	.	engineered_tag	7687	7711	.	+	0	ID=annotation2213996;Name=Help:Barcodes;Parent=BBa_C0070
BBa_I2030	.	CDS	7045	7647	.	+	0	ID=annotation301203;Name=rhlL;Parent=BBa_C0070
BBa_I2030	.	tag	7648	7680	.	+	0	ID=annotation306820;Name=LVA;Parent=BBa_C0070
>BBa_I2030
aaagaggagaaatactagatggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtgg
cgggtttaacgccgattgaggccaatggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattct
caatctcaccattcgcggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctg
ttcccgccaggagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtc
cgcgcgcctactggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgca
ccagccgcatttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcg
ataaatctgcttgagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcggg
tacgcgaggcttgtcagtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttg
cctgtcgccgtcgcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgc
atcagccaggcgaagctgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatc
aactctatttctcgcgagtatttaaaaaatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagt
gaatgatgtagccgtcaagttgtcagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctag
tgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttg
tttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcg
taacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagca
tttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccattactagagaaag
aggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaat
cgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataag
cgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagct
ggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacattt
aggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcacta
gagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcg
ctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcacca
aggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtggg
tccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccag
gcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctac
tagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgaca
tccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaaaccagtaacgttatacgat
gtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgc
gggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtc
gttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgcc
gatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatc
ttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctg
cactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggt
acgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtct
cggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcga
ctggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgcc
aacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgg
gatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggca
aaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtg
aaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcac
gacaggtttcccgactggaaagcgggcagtaataacactgatagtgctagtgtagatcactactagagccaggcatcaa
ataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtc
acactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttgg
ccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagtt
agctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataaca
atttcacacatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacag
gtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggcttta
cactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaa
atactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggt
ttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctt
tgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaaga
ttttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtaca
cggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatg
cattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaaga
agaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgca
gagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctg
caaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaagaggagaa
atactagatgatcgttcagatcggtcgtcgtgaagagttcgacaaaaaactgctgggtgaaatgcacaaactgcgtgct
caggttttcaaagaacgtaaaggttgggacgtttccgttatcgacgaaatggaaatcgacggttacgacgctctgtccc
cgtactacatgctgatccaggaagacaccccggaagctcaggttttcggttgctggcgtatcttcgacaccaccggtcc
gtacatgctgaaaaacaccttcccggaactgctgcacggtaaagaagctccgtgctccccgcacatctgggaactgtcc
cgtttcgctatcaactccggtcagaaaggttccctgggtttctccgactgcaccctggaagctatgcgtgctctggctc
gttactccttgcagaacgacatccagaccctggttaccgttaccaccgttggtgttgaaaaaatgatgatccgtgctgg
tctggacgtttcccgtttcggtccgcacctgaaaatcggtatcgaacgtgctgttgctctgcgtatcgaactgaacgct
aaaacccagatcgctctgtacggtggtgttctggttgaacagcgtctggctgtttccgctgctaacgacgaaaactacg
ctctggttgcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcag
tcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgg
gtgggcctttctgcgtttatatactagagaaagaggagaaatactagatggccttggttgacggttttcttgagctgga
acgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttc
ggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcatt
acgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttctgggaacc
gtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatg
ccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtt
tcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggactggccttcgaaca
tccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgg
gagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtga
cctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctcgctgcaaacgacgaaaactacgcttt
agtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcga
aagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgg
gcctttctgcgtttatatactagagaaagaggagaaatactagatgaccatgattacgccaagcgcgcaattaaccctc
actaaagggaacaaaagctggagctccaccgcggtggcggcagcactagagctagtggatcccccgggctgtagaaatt
cgatatcaagcttatcgataccgtcgacctcgagggggggcccggtacccaattcgccctatagtgagtcgtattacgc
gcgctcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacat
ccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaataata
atactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggt
gaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcccctcgctga
gcgcgtcccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaacatacagatttccg
gcgaaatcaaggctacctgccagttctggcaggtttggccgcgggttctttttggtacacgaaagctactagagaaaga
ggagaaatactagatgatcgaactgctgtccgaatccctggaaggtctgtccgctgctatgatcgctgaactgggtcgt
taccgtcaccaggttttcatcgaaaaactgggttgggacgttgtttccacctcccgtgttcgtgaccaggagttcgacc
agttcgaccacccgcagacccgttacatcgttgctatgtcccgtcagggtatctgcggttgcgctcgtctgctgccgac
caccgacgcttacctgctgaaagacgttttcgcttacctgtgctccgaaaccccgccgtccgacccgtccgtttgggaa
ctgtcccgttacgctgcttccgctgctgacgacccgcagctggctatgaaaatcttctggtcctccctccagtgcgctt
ggtacctgggtgcttcctccgttgttgctgttaccaccaccgctatggaacgttacttcgttcgtaacggtgttatcct
ccagcgtctgggtccgccgcagaaagttaaaggtgaaaccctggttgctatctccttcccggcttaccaggaacgtggt
ctggaaatgctgctgcgttaccacccggaatggctccagggtgttccgctgtccatggctgttgctgcaaacgacgaaa
actacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaag
gctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcac
cttcgggtgggcctttctgcgtttatatactagaggcccctcgctgagcgcgtcccggagctgggggcaacctagctgc
cacctgcttttctgctagctattccagcgaaaacatacagatttccggcgaaatcaaggctacctgccagttctggcag
gtttggccgcgggttctttttggtacacgaaagctactagagaaagaggagaaatactagatggctgaagcgcaaaatg
atcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggccaatggttatctcga
tttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggtggtgaaaaat
cagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactacggtcgtcatc
cggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatggcttaactggccgtc
aatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctgtttgggcaaatcatt
aacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgttactgcggcgcatgg
aagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcgatcacctggc
agacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcgccgtcgcgtctgtcacatcttttccgccag
cagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagccaggcgaagctgcttttgagcactacccgga
tgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcgagtatttaaaaaatgcaccgg
ggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcagctgcaaacgac
gaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacg
aaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggc
tcaccttcgggtgggcctttctgcgtttatatactagagtcctgtgaaatctggcagttaccgttagctttcgaattgg
ctaaaaagtgttctactagagaaagaggagaaatactagatgaaaccagtaacgttatacgatgtcgcagagtatgccg
gtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagc
ggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgtt
gccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgcca
gcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgt
cagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcg
ttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtgg
agcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtct
ggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtcc
ggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgc
tgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccga
agacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgc
ttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccc
tggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgact
ggaaagcgggcagtaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctc
agtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttc
gggtgggcctttctgcgtttatatactagagttaaatttcctcttttcaggccggaataactccctataatgcgccacc
atactagagaaagaggagaaatactagatgaggaatgacggaggctttttgctgtggtgggacggtttgcgtagcgaga
tgcagccgatccacgacagccagggcgtgttcgccgtcctggaaaaggaagtgcggcgcctgggcttcgattactacgc
ctatggcgtgcgccacacgattcccttcacccggccgaagaccgaggtccatggcacctatcccaaggcctggctggag
cgataccagatgcagaactacggggccgtggatccggcgatcctcaacggcctgcgctcctcggaaatggtggtctgga
gcgacagcctgttcgaccagagccggatgctctggaacgaggctcgcgattggggcctctgtgtcggcgcgaccttgcc
gatccgcgcgccgaacaatttgctcagcgtgctttccgtggcgcgcgaccagcagaacatctccagcttcgagcgcgag
gaaatccgcctgcggctgcgttgcatgatcgagttgctgacccagaagctgaccgacctggagcatccgatgctgatgt
ccaacccggtctgcctgagccatcgcgaacgcgagatcctgcaatggaccgccgacggcaagagttccggggaaatcgc
catcatcctgagcatctccgagagcacggtgaacttccaccacaagaacatccagaagaagttcgacgcgccgaacaag
acgctggctgccgcctacgccgcggcgctgggtctcatcgctgcaaacgacgaaaactacgctttagtagcttaataac
actgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggccttt
cgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgttt
ata