LOCUS       BBa_I20300               736 bp    DNA     linear       23-Dec-2024
DEFINITION  RBS from T7 gene 4, optimal aligned spacing + Z0301 venus YFP
ACCESSION   BBa_I20300
VERSION     BBa_I20300.1  
FEATURES             Location/Qualifiers
     RBS             1..16
                     /label=BBa_I20299
     misc_feature    20..55
                     /label=gtc ...gtt -> gta ... gtt codon shuffle
     misc_feature    17..736
                     /label=BBa_Z0301
ORIGIN
        1 tacgaggagg atgaagatgg taagcaaagg agaggaatta tttacaggag ttgttccgat
       61 cctggttgaa ctggacggtg acgttaacgg tcacaaattc tctgtttctg gtgaaggtga
      121 aggtgacgct acctacggta aactgaccct gaaactgatc tgcaccaccg gtaaactgcc
      181 ggttccgtgg ccgaccctcg taactaccct gggttacggt ctccaatgct tcgctcgtta
      241 cccggaccac atgaaacagc acgacttctt caaatctgct atgccggaag gttacgttca
      301 ggaacgtacc atcttcttca aagacgacgg taactacaaa acccgtgctg aagttaaatt
      361 cgaaggtgac accctggtta accgtatcga actgaaaggt atcgacttca aagaagacgg
      421 taacatcctg ggtcacaaac tggaatacaa ctacaactct cacaacgttt acatcaccgc
      481 tgacaaacag aaaaacggta tcaaagctaa cttcaaaatc cgtcacaaca tcgaagacgg
      541 tggtgttcag ctggctgacc actaccagca gaacacccca attggtgatg gcccagttct
      601 gctgccggac aaccactacc tgtcttacca gtctgctctg tctaaagacc cgaacgaaaa
      661 acgtgaccac atggttctgc tggagtttgt taccgctgct ggtatcaccc tgggtatgga
      721 cgaactgtac aaataa
//