BBa_I20300
1
BBa_I20300
RBS from T7 gene 4, optimal aligned spacing + Z0301 venus YFP
2010-06-20T11:00:00Z
2015-08-31T04:07:40Z
n/a
A composite of:
(1) BBa_I20299, a strong RBS derived from phage T7 gene 4, and
(2) BBa_Z0301, a venus YFP codon-optimized for E.coli expression
Note there are no Biobrick "scar" in between.
false
false
_11_
0
1950
11
Not in stock
false
See individual parts
false
Francois St-Pierre
component2071369
1
BBa_Z0301
component2071367
1
BBa_I20299
annotation2071369
1
BBa_Z0301
range2071369
1
17
736
annotation2071367
1
BBa_I20299
range2071367
1
1
16
BBa_Z0301
1
BBa_Z0301
Venus YFP - E. coli codon optimized
2006-06-20T11:00:00Z
2015-05-08T01:14:55Z
Venus Sequence obtained from Prof. Miyawaki. Codon optimized from Gene Design by Boeke Lab.
Brighter Variant than standard EYFP.
false
false
_11_
0
64
10
It's complicated
false
None really
false
Sriram Kosuri
annotation1881736
1
gtc ...gtt -> gta ... gtt codon shuffle
range1881736
1
4
39
BBa_I20299
1
BBa_I20299
RBS from T7 gene 4, optimal aligned spacing
2010-06-20T11:00:00Z
2015-08-31T04:07:40Z
Derived from the RBS of phage T7 gene 4
RBS derived from phage T7 gene 4. Similar to I20298, but with the last (3') nucleotides removed. This produces an optimal aligned spacing of 5 nucleotides between the Shine-Dalgarno (SD) sequence and the ATG of the following CDS, when the RBS is placed immediately before the ATG i.e. without Biobrick "scar". For information on "optimal aligned spacing", see Chen et al., (1994) NAR, 22(23):4953-7 [PMID 7528374]
false
false
_11_
0
1950
11
Not in stock
false
See "long description"
false
Francois St-Pierre
BBa_Z0301_sequence
1
atggtaagcaaaggagaggaattatttacaggagttgttccgatcctggttgaactggacggtgacgttaacggtcacaaattctctgtttctggtgaaggtgaaggtgacgctacctacggtaaactgaccctgaaactgatctgcaccaccggtaaactgccggttccgtggccgaccctcgtaactaccctgggttacggtctccaatgcttcgctcgttacccggaccacatgaaacagcacgacttcttcaaatctgctatgccggaaggttacgttcaggaacgtaccatcttcttcaaagacgacggtaactacaaaacccgtgctgaagttaaattcgaaggtgacaccctggttaaccgtatcgaactgaaaggtatcgacttcaaagaagacggtaacatcctgggtcacaaactggaatacaactacaactctcacaacgtttacatcaccgctgacaaacagaaaaacggtatcaaagctaacttcaaaatccgtcacaacatcgaagacggtggtgttcagctggctgaccactaccagcagaacaccccaattggtgatggcccagttctgctgccggacaaccactacctgtcttaccagtctgctctgtctaaagacccgaacgaaaaacgtgaccacatggttctgctggagtttgttaccgctgctggtatcaccctgggtatggacgaactgtacaaataa
BBa_I20300_sequence
1
tacgaggaggatgaagatggtaagcaaaggagaggaattatttacaggagttgttccgatcctggttgaactggacggtgacgttaacggtcacaaattctctgtttctggtgaaggtgaaggtgacgctacctacggtaaactgaccctgaaactgatctgcaccaccggtaaactgccggttccgtggccgaccctcgtaactaccctgggttacggtctccaatgcttcgctcgttacccggaccacatgaaacagcacgacttcttcaaatctgctatgccggaaggttacgttcaggaacgtaccatcttcttcaaagacgacggtaactacaaaacccgtgctgaagttaaattcgaaggtgacaccctggttaaccgtatcgaactgaaaggtatcgacttcaaagaagacggtaacatcctgggtcacaaactggaatacaactacaactctcacaacgtttacatcaccgctgacaaacagaaaaacggtatcaaagctaacttcaaaatccgtcacaacatcgaagacggtggtgttcagctggctgaccactaccagcagaacaccccaattggtgatggcccagttctgctgccggacaaccactacctgtcttaccagtctgctctgtctaaagacccgaacgaaaaacgtgaccacatggttctgctggagtttgttaccgctgctggtatcaccctgggtatggacgaactgtacaaataa
BBa_I20299_sequence
1
tacgaggaggatgaag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z