BBa_I20300 1 BBa_I20300 RBS from T7 gene 4, optimal aligned spacing + Z0301 venus YFP 2010-06-20T11:00:00Z 2015-08-31T04:07:40Z n/a A composite of: (1) BBa_I20299, a strong RBS derived from phage T7 gene 4, and (2) BBa_Z0301, a venus YFP codon-optimized for E.coli expression Note there are no Biobrick "scar" in between. false false _11_ 0 1950 11 Not in stock false See individual parts false Francois St-Pierre component2071369 1 BBa_Z0301 component2071367 1 BBa_I20299 annotation2071369 1 BBa_Z0301 range2071369 1 17 736 annotation2071367 1 BBa_I20299 range2071367 1 1 16 BBa_Z0301 1 BBa_Z0301 Venus YFP - E. coli codon optimized 2006-06-20T11:00:00Z 2015-05-08T01:14:55Z Venus Sequence obtained from Prof. Miyawaki. Codon optimized from Gene Design by Boeke Lab. Brighter Variant than standard EYFP. false false _11_ 0 64 10 It's complicated false None really false Sriram Kosuri annotation1881736 1 gtc ...gtt -> gta ... gtt codon shuffle range1881736 1 4 39 BBa_I20299 1 BBa_I20299 RBS from T7 gene 4, optimal aligned spacing 2010-06-20T11:00:00Z 2015-08-31T04:07:40Z Derived from the RBS of phage T7 gene 4 RBS derived from phage T7 gene 4. Similar to I20298, but with the last (3') nucleotides removed. This produces an optimal aligned spacing of 5 nucleotides between the Shine-Dalgarno (SD) sequence and the ATG of the following CDS, when the RBS is placed immediately before the ATG i.e. without Biobrick "scar". For information on "optimal aligned spacing", see Chen et al., (1994) NAR, 22(23):4953-7 [PMID 7528374] false false _11_ 0 1950 11 Not in stock false See "long description" false Francois St-Pierre BBa_Z0301_sequence 1 atggtaagcaaaggagaggaattatttacaggagttgttccgatcctggttgaactggacggtgacgttaacggtcacaaattctctgtttctggtgaaggtgaaggtgacgctacctacggtaaactgaccctgaaactgatctgcaccaccggtaaactgccggttccgtggccgaccctcgtaactaccctgggttacggtctccaatgcttcgctcgttacccggaccacatgaaacagcacgacttcttcaaatctgctatgccggaaggttacgttcaggaacgtaccatcttcttcaaagacgacggtaactacaaaacccgtgctgaagttaaattcgaaggtgacaccctggttaaccgtatcgaactgaaaggtatcgacttcaaagaagacggtaacatcctgggtcacaaactggaatacaactacaactctcacaacgtttacatcaccgctgacaaacagaaaaacggtatcaaagctaacttcaaaatccgtcacaacatcgaagacggtggtgttcagctggctgaccactaccagcagaacaccccaattggtgatggcccagttctgctgccggacaaccactacctgtcttaccagtctgctctgtctaaagacccgaacgaaaaacgtgaccacatggttctgctggagtttgttaccgctgctggtatcaccctgggtatggacgaactgtacaaataa BBa_I20300_sequence 1 tacgaggaggatgaagatggtaagcaaaggagaggaattatttacaggagttgttccgatcctggttgaactggacggtgacgttaacggtcacaaattctctgtttctggtgaaggtgaaggtgacgctacctacggtaaactgaccctgaaactgatctgcaccaccggtaaactgccggttccgtggccgaccctcgtaactaccctgggttacggtctccaatgcttcgctcgttacccggaccacatgaaacagcacgacttcttcaaatctgctatgccggaaggttacgttcaggaacgtaccatcttcttcaaagacgacggtaactacaaaacccgtgctgaagttaaattcgaaggtgacaccctggttaaccgtatcgaactgaaaggtatcgacttcaaagaagacggtaacatcctgggtcacaaactggaatacaactacaactctcacaacgtttacatcaccgctgacaaacagaaaaacggtatcaaagctaacttcaaaatccgtcacaacatcgaagacggtggtgttcagctggctgaccactaccagcagaacaccccaattggtgatggcccagttctgctgccggacaaccactacctgtcttaccagtctgctctgtctaaagacccgaacgaaaaacgtgaccacatggttctgctggagtttgttaccgctgctggtatcaccctgggtatggacgaactgtacaaataa BBa_I20299_sequence 1 tacgaggaggatgaag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z