BBa_R0184
1
BBa_R0184
T7 promoter (lacI repressible)
2007-04-25T11:00:00Z
2015-05-08T01:14:15Z
This sequence is based on the T7lac promoter described by Dubendorff and Studier (JMB 1991, 219, 45-59). The only modification is the mutation at -16.
A mutant version of the T7lac promoter designed by Dubendorff and Studier. The promoter is based on the strong ??10 T7 promoter but incorporates an A->C mutation at -16. The LacI operator site is a partially symmetric 25bp sequence.
false
false
_11_
0
135
84
Not in stock
false
The -16 mutation was included to weaken the consensus promoter as described by Imburgio and co-workers (see BBa_R0183 for more information)
false
Bartholomew Canton
annotation1932680
1
Center of binding site symmetry
range1932680
1
31
31
annotation1932679
1
lacI binding site
range1932679
1
20
44
annotation1932678
1
BBa_R0183 fragment
range1932678
1
1
20
BBa_B0032
1
BBa_B0032
RBS.3 (medium) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1710
1
RBS
range1710
1
7
10
annotation7027
1
BBa_B0032
range7027
1
1
13
annotation1709
1
RBS-3\Weak
range1709
1
1
13
BBa_I2033
1
BBa_I2033
lacI repressible T7 promoter and a weak E. coli RBS
2007-05-12T11:00:00Z
2015-08-31T04:07:40Z
composite part
This part is an intermediate test construct in an early version of a biological virtual machine (VM2.0). It is composed of a lacI repressible T7 promoter and an E. coli RBS. The composite part can be composed with a coding region which will be expressed in the presence of T7 RNA polymerase and in the absence of sufficient amounts of lacI repressor.
false
false
_11_
0
135
84
Not in stock
false
n/a
false
Bartholomew Canton
component2218383
1
BBa_R0184
component2218385
1
BBa_B0032
annotation2218383
1
BBa_R0184
range2218383
1
1
44
annotation2218385
1
BBa_B0032
range2218385
1
53
65
BBa_R0184_sequence
1
tcatacgactcactataggggaattgtgagcggataacaattcc
BBa_B0032_sequence
1
tcacacaggaaag
BBa_I2033_sequence
1
tcatacgactcactataggggaattgtgagcggataacaattcctactagagtcacacaggaaag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z