LOCUS       BBa_I2035                847 bp    DNA     linear       20-Nov-2024
DEFINITION  T7 RNAP dependent, lacI regulated GFP expression system
ACCESSION   BBa_I2035
VERSION     BBa_I2035.1  
FEATURES             Location/Qualifiers
     promoter        1..20
                     /label=BBa_R0183 fragment
     misc_feature    20..44
                     /label=lacI binding site
     misc_feature    31..31
                     /label=Center of binding site symmetry
     promoter        1..44
                     /label=BBa_R0184
     RBS             53..65
                     /label=RBS-3\Weak
     RBS             59..62
                     /label=RBS
     misc_feature    53..65
                     /label=BBa_B0032
     RBS             53..65
                     /label=BBa_B0032
     CDS             72..791
                     /label=GFP protein
     CDS             72..791
                     /label=BBa_E0040
     terminator      800..847
                     /label=BBa_B0016
     misc_feature    53..847
                     /label=BBa_E0241
ORIGIN
        1 tcatacgact cactataggg gaattgtgag cggataacaa ttcctactag agtcacacag
       61 gaaagtacta gatgcgtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg
      121 aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg
      181 caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat
      241 ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga tacccagatc
      301 atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa
      361 ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg
      421 atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc
      481 ttggacacaa attggaatac aactataact cacacaatgt atacatcatg gcagacaaac
      541 aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc
      601 aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag
      661 acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc
      721 acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat
      781 acaaataata atactagagc tagcataacc ccttggggcc tctaaacggg tcttgagggg
      841 ttttttg
//