BBa_I2037
1
BBa_I2037
mCherry reporter device and GFP reporter device in sequence
2007-05-22T11:00:00Z
2015-08-31T04:07:40Z
n/a
test
false
false
_11_
0
135
84
Not in stock
false
n/a
false
Bartholomew Canton
component2233660
1
BBa_E70202
component2233646
1
BBa_I20222
annotation2233646
1
BBa_I20222
range2233646
1
1
1113
annotation2233660
1
BBa_E70202
range2233660
1
1122
2055
BBa_S00162
1
BBa_S00162
mCherry + 3' Hairpin + Terminator
2007-03-06T12:00:00Z
2015-05-08T01:14:16Z
n/a
This is a theoretical intermediate part that will never be physically built, but is necessary for creating the part files of my RBS measurement devices which do not use Biobricks Cloning Sites for inserting the RBS sequence. This is the region found downstream of the RBS sequence in all of the mCherry measurement devices.
false
false
_11_
0
571
10
Not in stock
false
n/a
false
Heather Keller
component1920665
1
BBa_B0012
component1920662
1
BBa_B0101
component1920663
1
BBa_B0010
component1920660
1
BBa_J06504
annotation1920662
1
BBa_B0101
range1920662
1
723
780
annotation1920660
1
BBa_J06504
range1920660
1
1
714
annotation1920663
1
BBa_B0010
range1920663
1
789
868
annotation1920665
1
BBa_B0012
range1920665
1
877
917
BBa_B0104
1
AvrII/XbaI
AvrII/XbaI mixed site
2007-03-06T12:00:00Z
2015-08-31T04:07:21Z
n/a
This is the 6 bp mixed site that results when ligating the compatible ends (CTAG 5' overhang) that result from AvrII cleavage (CCTAGA) of the upstream part and XbaI (TCTAGA) cleavage of the downstream part.
false
false
_11_
0
571
10
Not in stock
false
n/a
false
Heather Keller
annotation1920669
1
AvrII/XbaI mixed site
range1920669
1
1
6
BBa_J06504
1
mCherry
monomeric RFP optimized for bacteria
2005-07-17T11:00:00Z
2016-01-25T01:05:28Z
mCherry from Roger Y. Tsien's lab, altered to be BioBrick compatible
Released HQ 2013
mRFP (DsRed) derived, altered to be a biobrick by removing a PstI site and adding in the ends.
false
false
_20_
4206
340
20
In stock
false
<p>
Made a point mutation to eliminate a PstI site in the middle and then added BioBrick ends using PCR.
</p>
<p>
Sequenced using primers that bind to the pSB1A2 plasmid on either side of the brick.
</p>
true
ytwang
annotation1585833
1
C->T (removing PstI site)
range1585833
1
352
352
annotation1585829
1
mCherry
range1585829
1
1
711
BBa_B2022
1
BBa_B2022
4B RBS from T7
2007-05-22T11:00:00Z
2015-08-31T04:07:21Z
to be added automatically later
to be added automatically later
false
false
_11_
0
135
84
Not in stock
false
to be added automatically later
false
Bartholomew Canton
BBa_E0040
1
GFP
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
2004-09-29T11:00:00Z
2016-01-26T02:09:38Z
Released HQ 2013
GFP (mut3b) [note that this part does not have a barcode]
false
true
_11_1_
4206
61
7
In stock
false
true
jcbraff
annotation1934520
1
GFP protein
range1934520
1
1
720
BBa_B0100
1
OmpA 5
OmpA 5
2007-02-28T12:00:00Z
2015-08-31T04:07:21Z
MG1655 genome, OmpA gene
5 prime UTR from the OmpA gene in E.coli used for stabilizing the downstream RNA transcript
false
false
_11_
0
571
10
Not in stock
false
This region was PCR amplified from the genome of MG1655.
false
Heather Keller
annotation1919682
1
ss1
range1919682
1
64
74
annotation1919681
1
hp1
range1919681
1
1
63
annotation1919684
1
ss2
range1919684
1
104
115
annotation1919683
1
hp2
range1919683
1
75
103
BBa_E70202
1
BBa_E70202
Reporter for Dedicated Translation
2006-05-01T11:00:00Z
2015-08-31T04:07:27Z
-- No description --
false
false
_11_6_
0
135
6
Not in stock
false
false
Bartholomew Canton
component1911779
1
BBa_E0040
component1911782
1
BBa_B0012
component1911778
1
BBa_B0073
component1911780
1
BBa_B0010
component1911774
1
BBa_R0040
annotation1911782
1
BBa_B0012
range1911782
1
894
934
annotation1911778
1
BBa_B0073
range1911778
1
63
71
annotation1911780
1
BBa_B0010
range1911780
1
806
885
annotation1911779
1
BBa_E0040
range1911779
1
78
797
annotation1911774
1
BBa_R0040
range1911774
1
1
54
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_S00164
1
BBa_S00164
R0011 + B0100 + AvrII/XbaI scar
2007-03-06T12:00:00Z
2015-05-08T01:14:16Z
n/a
This is a theoretical intermediate part that will never be physically built, but is necessary for creating the part files of my RBS measurement devices which do not use Biobricks Cloning Sites for inserting the RBS sequence. This is the region found upstream of the RBS sequence in all of the GFP and mCherry measurement devices, including the AvrII/XbaI scar site that is created between this part and the RBS during cloning.
false
false
_11_
0
571
10
Not in stock
false
n/a
false
Heather Keller
component1920713
1
BBa_B0104
component1920702
1
BBa_R0011
component1920711
1
BBa_B0100
annotation1920711
1
BBa_B0100
range1920711
1
56
170
annotation1920713
1
BBa_B0104
range1920713
1
171
176
annotation1920702
1
BBa_R0011
range1920702
1
1
54
BBa_I20222
1
BBa_I20222
4B RBS from T7 charactization device
2007-05-22T11:00:00Z
2015-08-31T04:07:39Z
to be added automatially later
to be added automatially later
false
false
_11_
0
135
84
Not in stock
false
to be added automatially later
false
Bartholomew Canton
component2226384
1
BBa_B2022
component2226396
1
BBa_S00162
component2226383
1
BBa_S00164
annotation2226383
1
BBa_S00164
range2226383
1
1
176
annotation2226396
1
BBa_S00162
range2226396
1
197
1113
annotation2226384
1
BBa_B2022
range2226384
1
177
196
BBa_R0040
1
p(tetR)
TetR repressible promoter
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210.
Released HQ 2013
Sequence for pTet inverting regulator driven by the TetR protein.</P>
false
true
_1_
0
24
7
In stock
false
<P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P>
true
June Rhee, Connie Tao, Ty Thomson, Louis Waldman
annotation1986785
1
-35
range1986785
1
20
25
annotation1986783
1
TetR 1
range1986783
1
1
19
annotation1986784
1
BBa_R0040
range1986784
1
1
54
annotation1986787
1
-10
range1986787
1
43
48
annotation1986786
1
TetR 2
range1986786
1
26
44
BBa_B0101
1
HP3 3
HP3 3
2007-02-28T12:00:00Z
2015-08-31T04:07:21Z
The part was made by primer annealing.
3' stabilizing mRNA designed by Christina Smolke and Jay Keasling for stabilizing gfp.
false
false
_11_
0
571
10
Not in stock
false
A single base pair change (A to T at position 25) from the original design was made in order to remove a PstI site occurring in the loop of the hairpin. This should have no effect on the secondary structure.
false
Heather Keller
annotation1919685
1
stem_loop
range1919685
1
6
58
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
BBa_R0011
1
lacI+pL
Promoter (lacI regulated, lambda pL hybrid)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
represillator of Elowitz and Leibler (2000)
Released HQ 2013
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
false
true
_1_
0
24
7
In stock
false
<P> <P>hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site.<P>LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
true
Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
annotation2001
1
lac O1
range2001
1
26
42
annotation7064
1
BBa_R0011
range7064
1
1
54
annotation1999
1
lac O1
range1999
1
3
19
annotation2000
1
-35
range2000
1
20
25
annotation2002
1
-10
range2002
1
43
48
BBa_B0073
1
BBa_B0073
Specialized RBS
2006-05-01T11:00:00Z
2015-08-31T04:07:21Z
-- No description --
false
false
_11_6_
0
135
6
Not in stock
false
false
Bartholomew Canton
BBa_B0100_sequence
1
gccaggggtgctcggcataagccgaagatatcggtagagttaatattgagcagatcccccggtgaaggatttaaccgtgttatctcgttggagatattcatggcgtattttggat
BBa_E70202_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacaccactactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0104_sequence
1
cctaga
BBa_S00162_sequence
1
atggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataatactagaggatcgcctgatcccggtgcacccgggcagctgcatagtctgggtgcaccgggatcaggtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0073_sequence
1
tcacaccac
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_J06504_sequence
1
atggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataa
BBa_R0040_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
BBa_I2037_sequence
1
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacagccaggggtgctcggcataagccgaagatatcggtagagttaatattgagcagatcccccggtgaaggatttaaccgtgttatctcgttggagatattcatggcgtattttggatcctagaaaccctcaggaggtaaaccaatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataatactagaggatcgcctgatcccggtgcacccgggcagctgcatagtctgggtgcaccgggatcaggtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacaccactactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_S00164_sequence
1
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacagccaggggtgctcggcataagccgaagatatcggtagagttaatattgagcagatcccccggtgaaggatttaaccgtgttatctcgttggagatattcatggcgtattttggatcctaga
BBa_B0101_sequence
1
gatcgcctgatcccggtgcacccgggcagctgcatagtctgggtgcaccgggatcagg
BBa_E0040_sequence
1
atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
BBa_B2022_sequence
1
aaccctcaggaggtaaacca
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_I20222_sequence
1
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacagccaggggtgctcggcataagccgaagatatcggtagagttaatattgagcagatcccccggtgaaggatttaaccgtgttatctcgttggagatattcatggcgtattttggatcctagaaaccctcaggaggtaaaccaatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataatactagaggatcgcctgatcccggtgcacccgggcagctgcatagtctgggtgcaccgggatcaggtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_R0011_sequence
1
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z