BBa_I2037
type: ComponentDefinition
persistent identity: BBa I2037
display id: BBa_I2037
version: 1
was generated by: iGEM to SBOL conversion
description: mCherry reporter device and GFP reporter device in sequence
created: 2007-05-22T11:00:00Z
modified: 2015-08-31T04:07:40Z
mutable provenance: n/a
owned by: myers
owned by: james
mutable description: test
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: n/a
star: false
creator: Bartholomew Canton
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E70202
component: BBa_I20222
sequence annotation: BBa_I20222
sequence annotation: BBa_E70202
sequence: https://synbiohub.org/public/igem/BBa_I2037_sequence/1

BBa_E70202
type: Component
persistent identity: component2233660
display id: component2233660
version: 1
top level: BBa_I2037
definition: BBa_E70202
access: public

BBa_I20222
type: Component
persistent identity: component2233646
display id: component2233646
version: 1
top level: BBa_I2037
definition: BBa_I20222
access: public

BBa_I20222
type: SequenceAnnotation
persistent identity: annotation2233646
display id: annotation2233646
version: 1
top level: BBa_I2037
location: https://synbiohub.org/public/igem/BBa_I2037/annotation2233646/range2233646/1
component: BBa_I20222

https://synbiohub.org/public/igem/BBa_I2037/annotation2233646/range2233646/1
type: Range
persistent identity: range2233646
display id: range2233646
version: 1
top level: BBa_I2037
direction: direction/reverse
start: 1
end: 1113
orientation: inline

BBa_E70202
type: SequenceAnnotation
persistent identity: annotation2233660
display id: annotation2233660
version: 1
top level: BBa_I2037
location: https://synbiohub.org/public/igem/BBa_I2037/annotation2233660/range2233660/1
component: BBa_E70202

https://synbiohub.org/public/igem/BBa_I2037/annotation2233660/range2233660/1
type: Range
persistent identity: range2233660
display id: range2233660
version: 1
top level: BBa_I2037
direction: direction/reverse
start: 1122
end: 2055
orientation: inline

BBa_E70202
type: ComponentDefinition
persistent identity: BBa E70202
display id: BBa_E70202
version: 1
was generated by: iGEM to SBOL conversion
description: Reporter for Dedicated Translation
created: 2006-05-01T11:00:00Z
modified: 2015-08-31T04:07:27Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: false
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Bartholomew Canton
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0010
component: BBa_E0040
component: BBa_B0012
component: BBa_B0073
component: BBa_R0040
sequence annotation: BBa_R0040
sequence annotation: BBa_B0073
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_E70202_sequence/1

BBa_B0010
type: Component
persistent identity: component1911780
display id: component1911780
version: 1
top level: BBa_E70202
definition: BBa_B0010
access: public

BBa_E0040
type: Component
persistent identity: component1911779
display id: component1911779
version: 1
top level: BBa_E70202
definition: GFP
access: public

BBa_B0012
type: Component
persistent identity: component1911782
display id: component1911782
version: 1
top level: BBa_E70202
definition: BBa_B0012
access: public

BBa_B0073
type: Component
persistent identity: component1911778
display id: component1911778
version: 1
top level: BBa_E70202
definition: BBa_B0073
access: public

BBa_R0040
type: Component
persistent identity: component1911774
display id: component1911774
version: 1
top level: BBa_E70202
definition: p(tetR)
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1911774
display id: annotation1911774
version: 1
top level: BBa_E70202
location: https://synbiohub.org/public/igem/BBa_E70202/annotation1911774/range1911774/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_E70202/annotation1911774/range1911774/1
type: Range
persistent identity: range1911774
display id: range1911774
version: 1
top level: BBa_E70202
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0073
type: SequenceAnnotation
persistent identity: annotation1911778
display id: annotation1911778
version: 1
top level: BBa_E70202
location: https://synbiohub.org/public/igem/BBa_E70202/annotation1911778/range1911778/1
component: BBa_B0073

https://synbiohub.org/public/igem/BBa_E70202/annotation1911778/range1911778/1
type: Range
persistent identity: range1911778
display id: range1911778
version: 1
top level: BBa_E70202
direction: direction/reverse
start: 63
end: 71
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1911779
display id: annotation1911779
version: 1
top level: BBa_E70202
location: https://synbiohub.org/public/igem/BBa_E70202/annotation1911779/range1911779/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_E70202/annotation1911779/range1911779/1
type: Range
persistent identity: range1911779
display id: range1911779
version: 1
top level: BBa_E70202
direction: direction/reverse
start: 78
end: 797
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1911780
display id: annotation1911780
version: 1
top level: BBa_E70202
location: https://synbiohub.org/public/igem/BBa_E70202/annotation1911780/range1911780/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_E70202/annotation1911780/range1911780/1
type: Range
persistent identity: range1911780
display id: range1911780
version: 1
top level: BBa_E70202
direction: direction/forward
start: 806
end: 885
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1911782
display id: annotation1911782
version: 1
top level: BBa_E70202
location: https://synbiohub.org/public/igem/BBa_E70202/annotation1911782/range1911782/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_E70202/annotation1911782/range1911782/1
type: Range
persistent identity: range1911782
display id: range1911782
version: 1
top level: BBa_E70202
direction: direction/forward
start: 894
end: 934
orientation: inline

BBa_I20222
type: ComponentDefinition
persistent identity: BBa I20222
display id: BBa_I20222
version: 1
was generated by: iGEM to SBOL conversion
description: 4B RBS from T7 charactization device
created: 2007-05-22T11:00:00Z
modified: 2015-08-31T04:07:39Z
mutable provenance: to be added automatially later
owned by: james
owned by: myers
mutable description: to be added automatially later
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: to be added automatially later
star: false
creator: Bartholomew Canton
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_S00164
component: BBa_S00162
component: BBa_B2022
sequence annotation: BBa_S00164
sequence annotation: BBa_B2022
sequence annotation: BBa_S00162
sequence: https://synbiohub.org/public/igem/BBa_I20222_sequence/1

BBa_S00164
type: Component
persistent identity: component2226383
display id: component2226383
version: 1
top level: BBa_I20222
definition: BBa_S00164
access: public

BBa_S00162
type: Component
persistent identity: component2226396
display id: component2226396
version: 1
top level: BBa_I20222
definition: BBa_S00162
access: public

BBa_B2022
type: Component
persistent identity: component2226384
display id: component2226384
version: 1
top level: BBa_I20222
definition: BBa_B2022
access: public

BBa_S00164
type: SequenceAnnotation
persistent identity: annotation2226383
display id: annotation2226383
version: 1
top level: BBa_I20222
location: https://synbiohub.org/public/igem/BBa_I20222/annotation2226383/range2226383/1
component: BBa_S00164

https://synbiohub.org/public/igem/BBa_I20222/annotation2226383/range2226383/1
type: Range
persistent identity: range2226383
display id: range2226383
version: 1
top level: BBa_I20222
direction: direction/reverse
start: 1
end: 176
orientation: inline

BBa_B2022
type: SequenceAnnotation
persistent identity: annotation2226384
display id: annotation2226384
version: 1
top level: BBa_I20222
location: https://synbiohub.org/public/igem/BBa_I20222/annotation2226384/range2226384/1
component: BBa_B2022

https://synbiohub.org/public/igem/BBa_I20222/annotation2226384/range2226384/1
type: Range
persistent identity: range2226384
display id: range2226384
version: 1
top level: BBa_I20222
direction: direction/reverse
start: 177
end: 196
orientation: inline

BBa_S00162
type: SequenceAnnotation
persistent identity: annotation2226396
display id: annotation2226396
version: 1
top level: BBa_I20222
location: https://synbiohub.org/public/igem/BBa_I20222/annotation2226396/range2226396/1
component: BBa_S00162

https://synbiohub.org/public/igem/BBa_I20222/annotation2226396/range2226396/1
type: Range
persistent identity: range2226396
display id: range2226396
version: 1
top level: BBa_I20222
direction: direction/reverse
start: 197
end: 1113
orientation: inline

BBa_S00162
type: ComponentDefinition
persistent identity: BBa S00162
display id: BBa_S00162
version: 1
was generated by: iGEM to SBOL conversion
description: mCherry + 3' Hairpin + Terminator
created: 2007-03-06T12:00:00Z
modified: 2015-05-08T01:14:16Z
mutable provenance: n/a
owned by: james
owned by: myers
mutable description: This is a theoretical intermediate part that will never be physically built, but is necessary for creating the part files of my RBS measurement devices which do not use Biobricks Cloning Sites for inserting the RBS sequence. This is the region found downstream of the RBS sequence in all of the mCherry measurement devices.
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 571
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: n/a
star: false
creator: Heather Keller
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0010
component: BBa_B0101
component: BBa_J06504
component: BBa_B0012
sequence annotation: BBa_J06504
sequence annotation: BBa_B0101
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_S00162_sequence/1

BBa_B0010
type: Component
persistent identity: component1920663
display id: component1920663
version: 1
top level: BBa_S00162
definition: BBa_B0010
access: public

BBa_B0101
type: Component
persistent identity: component1920662
display id: component1920662
version: 1
top level: BBa_S00162
definition: HP3 3
access: public

BBa_J06504
type: Component
persistent identity: component1920660
display id: component1920660
version: 1
top level: BBa_S00162
definition: mCherry
access: public

BBa_B0012
type: Component
persistent identity: component1920665
display id: component1920665
version: 1
top level: BBa_S00162
definition: BBa_B0012
access: public

BBa_J06504
type: SequenceAnnotation
persistent identity: annotation1920660
display id: annotation1920660
version: 1
top level: BBa_S00162
location: https://synbiohub.org/public/igem/BBa_S00162/annotation1920660/range1920660/1
component: BBa_J06504

https://synbiohub.org/public/igem/BBa_S00162/annotation1920660/range1920660/1
type: Range
persistent identity: range1920660
display id: range1920660
version: 1
top level: BBa_S00162
direction: direction/reverse
start: 1
end: 714
orientation: inline

BBa_B0101
type: SequenceAnnotation
persistent identity: annotation1920662
display id: annotation1920662
version: 1
top level: BBa_S00162
location: https://synbiohub.org/public/igem/BBa_S00162/annotation1920662/range1920662/1
component: BBa_B0101

https://synbiohub.org/public/igem/BBa_S00162/annotation1920662/range1920662/1
type: Range
persistent identity: range1920662
display id: range1920662
version: 1
top level: BBa_S00162
direction: direction/reverse
start: 723
end: 780
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1920663
display id: annotation1920663
version: 1
top level: BBa_S00162
location: https://synbiohub.org/public/igem/BBa_S00162/annotation1920663/range1920663/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_S00162/annotation1920663/range1920663/1
type: Range
persistent identity: range1920663
display id: range1920663
version: 1
top level: BBa_S00162
direction: direction/forward
start: 789
end: 868
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1920665
display id: annotation1920665
version: 1
top level: BBa_S00162
location: https://synbiohub.org/public/igem/BBa_S00162/annotation1920665/range1920665/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S00162/annotation1920665/range1920665/1
type: Range
persistent identity: range1920665
display id: range1920665
version: 1
top level: BBa_S00162
direction: direction/forward
start: 877
end: 917
orientation: inline

BBa_S00164
type: ComponentDefinition
persistent identity: BBa S00164
display id: BBa_S00164
version: 1
was generated by: iGEM to SBOL conversion
description: R0011 + B0100 + AvrII/XbaI scar
created: 2007-03-06T12:00:00Z
modified: 2015-05-08T01:14:16Z
mutable provenance: n/a
owned by: james
owned by: myers
mutable description: This is a theoretical intermediate part that will never be physically built, but is necessary for creating the part files of my RBS measurement devices which do not use Biobricks Cloning Sites for inserting the RBS sequence. This is the region found upstream of the RBS sequence in all of the GFP and mCherry measurement devices, including the AvrII/XbaI scar site that is created between this part and the RBS during cloning.
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 571
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: n/a
star: false
creator: Heather Keller
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_B0104
component: BBa_B0100
component: BBa_R0011
sequence annotation: BBa_R0011
sequence annotation: BBa_B0100
sequence annotation: BBa_B0104
sequence: https://synbiohub.org/public/igem/BBa_S00164_sequence/1

BBa_B0104
type: Component
persistent identity: component1920713
display id: component1920713
version: 1
top level: BBa_S00164
definition: AvrII/XbaI
access: public

BBa_B0100
type: Component
persistent identity: component1920711
display id: component1920711
version: 1
top level: BBa_S00164
definition: OmpA 5
access: public

BBa_R0011
type: Component
persistent identity: component1920702
display id: component1920702
version: 1
top level: BBa_S00164
definition: lacI+pL
access: public

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1920702
display id: annotation1920702
version: 1
top level: BBa_S00164
location: https://synbiohub.org/public/igem/BBa_S00164/annotation1920702/range1920702/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_S00164/annotation1920702/range1920702/1
type: Range
persistent identity: range1920702
display id: range1920702
version: 1
top level: BBa_S00164
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0100
type: SequenceAnnotation
persistent identity: annotation1920711
display id: annotation1920711
version: 1
top level: BBa_S00164
location: https://synbiohub.org/public/igem/BBa_S00164/annotation1920711/range1920711/1
component: BBa_B0100

https://synbiohub.org/public/igem/BBa_S00164/annotation1920711/range1920711/1
type: Range
persistent identity: range1920711
display id: range1920711
version: 1
top level: BBa_S00164
direction: direction/reverse
start: 56
end: 170
orientation: inline

BBa_B0104
type: SequenceAnnotation
persistent identity: annotation1920713
display id: annotation1920713
version: 1
top level: BBa_S00164
location: https://synbiohub.org/public/igem/BBa_S00164/annotation1920713/range1920713/1
component: BBa_B0104

https://synbiohub.org/public/igem/BBa_S00164/annotation1920713/range1920713/1
type: Range
persistent identity: range1920713
display id: range1920713
version: 1
top level: BBa_S00164
direction: direction/reverse
start: 171
end: 176
orientation: inline

BBa_B2022
type: ComponentDefinition
persistent identity: BBa B2022
display id: BBa_B2022
version: 1
was generated by: iGEM to SBOL conversion
description: 4B RBS from T7
created: 2007-05-22T11:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: to be added automatically later
owned by: james
owned by: myers
mutable description: to be added automatically later
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: to be added automatically later
star: false
creator: Bartholomew Canton
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_B2022_sequence/1

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_B0073
type: ComponentDefinition
persistent identity: BBa B0073
display id: BBa_B0073
version: 1
was generated by: iGEM to SBOL conversion
description: Specialized RBS
created: 2006-05-01T11:00:00Z
modified: 2015-08-31T04:07:21Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Bartholomew Canton
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_B0073_sequence/1

OmpA 5
type: ComponentDefinition
persistent identity: BBa B0100
display id: BBa_B0100
version: 1
was generated by: iGEM to SBOL conversion
description: OmpA 5
created: 2007-02-28T12:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: MG1655 genome, OmpA gene
owned by: james
owned by: myers
mutable description: 5 prime UTR from the OmpA gene in E.coli used for stabilizing the downstream RNA transcript
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 571
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This region was PCR amplified from the genome of MG1655.
star: false
creator: Heather Keller
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: hp1
sequence annotation: ss1
sequence annotation: hp2
sequence annotation: ss2
sequence: https://synbiohub.org/public/igem/BBa_B0100_sequence/1

hp1
type: SequenceAnnotation
persistent identity: annotation1919681
display id: annotation1919681
version: 1
top level: OmpA 5
location: https://synbiohub.org/public/igem/BBa_B0100/annotation1919681/range1919681/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0100/annotation1919681/range1919681/1
type: Range
persistent identity: range1919681
display id: range1919681
version: 1
top level: OmpA 5
direction: direction/forward
start: 1
end: 63
orientation: inline

ss1
type: SequenceAnnotation
persistent identity: annotation1919682
display id: annotation1919682
version: 1
top level: OmpA 5
location: https://synbiohub.org/public/igem/BBa_B0100/annotation1919682/range1919682/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_B0100/annotation1919682/range1919682/1
type: Range
persistent identity: range1919682
display id: range1919682
version: 1
top level: OmpA 5
direction: direction/forward
start: 64
end: 74
orientation: inline

hp2
type: SequenceAnnotation
persistent identity: annotation1919683
display id: annotation1919683
version: 1
top level: OmpA 5
location: https://synbiohub.org/public/igem/BBa_B0100/annotation1919683/range1919683/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0100/annotation1919683/range1919683/1
type: Range
persistent identity: range1919683
display id: range1919683
version: 1
top level: OmpA 5
direction: direction/forward
start: 75
end: 103
orientation: inline

ss2
type: SequenceAnnotation
persistent identity: annotation1919684
display id: annotation1919684
version: 1
top level: OmpA 5
location: https://synbiohub.org/public/igem/BBa_B0100/annotation1919684/range1919684/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_B0100/annotation1919684/range1919684/1
type: Range
persistent identity: range1919684
display id: range1919684
version: 1
top level: OmpA 5
direction: direction/forward
start: 104
end: 115
orientation: inline

HP3 3
type: ComponentDefinition
persistent identity: BBa B0101
display id: BBa_B0101
version: 1
was generated by: iGEM to SBOL conversion
description: HP3 3
created: 2007-02-28T12:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: The part was made by primer annealing.
owned by: james
owned by: myers
mutable description: 3' stabilizing mRNA designed by Christina Smolke and Jay Keasling for stabilizing gfp.
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 571
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: A single base pair change (A to T at position 25) from the original design was made in order to remove a PstI site occurring in the loop of the hairpin. This should have no effect on the secondary structure.
star: false
creator: Heather Keller
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0101_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation1919685
display id: annotation1919685
version: 1
top level: HP3 3
location: https://synbiohub.org/public/igem/BBa_B0101/annotation1919685/range1919685/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0101/annotation1919685/range1919685/1
type: Range
persistent identity: range1919685
display id: range1919685
version: 1
top level: HP3 3
direction: direction/forward
start: 6
end: 58
orientation: inline

AvrII/XbaI
type: ComponentDefinition
persistent identity: BBa B0104
display id: BBa_B0104
version: 1
was generated by: iGEM to SBOL conversion
description: AvrII/XbaI mixed site
created: 2007-03-06T12:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: n/a
owned by: james
owned by: myers
mutable description: This is the 6 bp mixed site that results when ligating the compatible ends (CTAG 5' overhang) that result from AvrII cleavage (CCTAGA) of the upstream part and XbaI (TCTAGA) cleavage of the downstream part.
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 571
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: n/a
star: false
creator: Heather Keller
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: AvrII/XbaI mixed site
sequence: https://synbiohub.org/public/igem/BBa_B0104_sequence/1

AvrII/XbaI mixed site
type: SequenceAnnotation
persistent identity: annotation1920669
display id: annotation1920669
version: 1
top level: AvrII/XbaI
location: https://synbiohub.org/public/igem/BBa_B0104/annotation1920669/range1920669/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_B0104/annotation1920669/range1920669/1
type: Range
persistent identity: range1920669
display id: range1920669
version: 1
top level: AvrII/XbaI
direction: direction/forward
start: 1
end: 6
orientation: inline

mCherry
type: ComponentDefinition
persistent identity: BBa J06504
display id: BBa_J06504
version: 1
was generated by: iGEM to SBOL conversion
description: monomeric RFP optimized for bacteria
created: 2005-07-17T11:00:00Z
modified: 2016-01-25T01:05:28Z
mutable provenance: mCherry from Roger Y. Tsien's lab, altered to be BioBrick compatible
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: mRFP (DsRed) derived, altered to be a biobrick by removing a PstI site and adding in the ends.
discontinued: false
dominant: false
experience: experience/None
group u list: _20_
m user id: 4206
owner id: 340
owning group id: 20
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Made a point mutation to eliminate a PstI site in the middle and then added BioBrick ends using PCR. Sequenced using primers that bind to the pSB1A2 plasmid on either side of the brick.
star: true
creator: ytwang
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: mCherry
sequence annotation: C->T (removing PstI site)
sequence: https://synbiohub.org/public/igem/BBa_J06504_sequence/1

mCherry
type: SequenceAnnotation
persistent identity: annotation1585829
display id: annotation1585829
version: 1
top level: mCherry
location: https://synbiohub.org/public/igem/BBa_J06504/annotation1585829/range1585829/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_J06504/annotation1585829/range1585829/1
type: Range
persistent identity: range1585829
display id: range1585829
version: 1
top level: mCherry
direction: direction/forward
start: 1
end: 711
orientation: inline

C->T (removing PstI site)
type: SequenceAnnotation
persistent identity: annotation1585833
display id: annotation1585833
version: 1
top level: mCherry
location: https://synbiohub.org/public/igem/BBa_J06504/annotation1585833/range1585833/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_J06504/annotation1585833/range1585833/1
type: Range
persistent identity: range1585833
display id: range1585833
version: 1
top level: mCherry
direction: direction/forward
start: 352
end: 352
orientation: inline

lacI+pL
type: ComponentDefinition
persistent identity: BBa R0011
display id: BBa_R0011
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (lacI regulated, lambda pL hybrid)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site. LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
star: true
creator: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0011
sequence annotation: lac O1
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0011_sequence/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation7064
display id: annotation7064
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
type: Range
persistent identity: range7064
display id: range7064
version: 1
top level: lacI+pL
direction: direction/forward
start: 1
end: 54
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation1999
display id: annotation1999
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
type: Range
persistent identity: range1999
display id: range1999
version: 1
top level: lacI+pL
direction: direction/forward
start: 3
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2000
display id: annotation2000
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
type: Range
persistent identity: range2000
display id: range2000
version: 1
top level: lacI+pL
direction: direction/forward
start: 20
end: 25
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2001
display id: annotation2001
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
type: Range
persistent identity: range2001
display id: range2001
version: 1
top level: lacI+pL
direction: direction/forward
start: 26
end: 42
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002
display id: annotation2002
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
type: Range
persistent identity: range2002
display id: range2002
version: 1
top level: lacI+pL
direction: direction/forward
start: 43
end: 48
orientation: inline

https://synbiohub.org/public/igem/BBa_I2037_sequence/1
type: Sequence
persistent identity: BBa I2037 sequence
display id: BBa_I2037_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacagccaggggtgctcggcataagccgaagatatcggtagagttaatattgagcagatcccccggtgaaggatttaaccgtgttatctcgttggagatattcatggcgtattttggatcctagaaaccctcaggaggtaaaccaatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataatactagaggatcgcctgatcccggtgcacccgggcagctgcatagtctgggtgcaccgggatcaggtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacaccactactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaac…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E70202_sequence/1
type: Sequence
persistent identity: BBa E70202 sequence
display id: BBa_E70202_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacaccactactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I20222_sequence/1
type: Sequence
persistent identity: BBa I20222 sequence
display id: BBa_I20222_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacagccaggggtgctcggcataagccgaagatatcggtagagttaatattgagcagatcccccggtgaaggatttaaccgtgttatctcgttggagatattcatggcgtattttggatcctagaaaccctcaggaggtaaaccaatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataatactagaggatcgcctgatcccggtgcacccgggcagctgcatagtctgggtgcaccgggatcaggtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S00162_sequence/1
type: Sequence
persistent identity: BBa S00162 sequence
display id: BBa_S00162_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataatactagaggatcgcctgatcccggtgcacccgggcagctgcatagtctgggtgcaccgggatcaggtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S00164_sequence/1
type: Sequence
persistent identity: BBa S00164 sequence
display id: BBa_S00164_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacagccaggggtgctcggcataagccgaagatatcggtagagttaatattgagcagatcccccggtgaaggatttaaccgtgttatctcgttggagatattcatggcgtattttggatcctaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B2022_sequence/1
type: Sequence
persistent identity: BBa B2022 sequence
display id: BBa_B2022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaccctcaggaggtaaacca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0073_sequence/1
type: Sequence
persistent identity: BBa B0073 sequence
display id: BBa_B0073_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacaccac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0100_sequence/1
type: Sequence
persistent identity: BBa B0100 sequence
display id: BBa_B0100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gccaggggtgctcggcataagccgaagatatcggtagagttaatattgagcagatcccccggtgaaggatttaaccgtgttatctcgttggagatattcatggcgtattttggat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0101_sequence/1
type: Sequence
persistent identity: BBa B0101 sequence
display id: BBa_B0101_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gatcgcctgatcccggtgcacccgggcagctgcatagtctgggtgcaccgggatcagg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0104_sequence/1
type: Sequence
persistent identity: BBa B0104 sequence
display id: BBa_B0104_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cctaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J06504_sequence/1
type: Sequence
persistent identity: BBa J06504 sequence
display id: BBa_J06504_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0011_sequence/1
type: Sequence
persistent identity: BBa R0011 sequence
display id: BBa_R0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z