##gff-version 3
##sequence-region BBa_I2038 1 2055
BBa_I2038	.	engineered_region	1	1113	.	+	0	ID=BBa_I20240;Name=BBa_I20240
BBa_I2038	.	engineered_region	197	1113	.	+	0	ID=BBa_S00162;Name=BBa_S00162;Parent=BBa_I20240
BBa_I2038	.	mature_transcript_region	919	976	.	+	0	ID=BBa_B0101;Name=BBa_B0101;Parent=BBa_S00162
BBa_I2038	.	stem_loop	924	976	.	+	0	ID=annotation1919685;Name=stem_loop;Parent=BBa_B0101
BBa_I2038	.	engineered_region	197	910	.	+	0	ID=BBa_J06504;Name=BBa_J06504;Parent=BBa_S00162
BBa_I2038	.	sequence_alteration	548	548	.	+	0	ID=annotation1585833;Name=C->T (removing PstI site);Parent=BBa_J06504
BBa_I2038	.	CDS	197	907	.	+	0	ID=annotation1585829;Name=mCherry;Parent=BBa_J06504
BBa_I2038	.	terminator	985	1064	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_S00162
BBa_I2038	.	stem_loop	996	1039	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I2038	.	engineered_region	985	1064	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I2038	.	terminator	1073	1113	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_S00162
BBa_I2038	.	polyA_site	1100	1113	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I2038	.	stem_loop	1080	1099	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I2038	.	engineered_region	1073	1113	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I2038	.	stop_codon	1106	1106	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I2038	.	engineered_region	1	176	.	+	0	ID=BBa_S00164;Name=BBa_S00164;Parent=BBa_I20240
BBa_I2038	.	mature_transcript_region	56	170	.	+	0	ID=BBa_B0100;Name=BBa_B0100;Parent=BBa_S00164
BBa_I2038	.	stem_loop	56	118	.	+	0	ID=annotation1919681;Name=hp1;Parent=BBa_B0100
BBa_I2038	.	stem_loop	130	158	.	+	0	ID=annotation1919683;Name=hp2;Parent=BBa_B0100
BBa_I2038	.	sequence_feature	119	129	.	+	0	ID=annotation1919682;Name=ss1;Parent=BBa_B0100
BBa_I2038	.	sequence_feature	159	170	.	+	0	ID=annotation1919684;Name=ss2;Parent=BBa_B0100
BBa_I2038	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_S00164
BBa_I2038	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_I2038	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_I2038	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_I2038	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_I2038	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_I2038	.	sequence_feature	171	176	.	+	0	ID=BBa_B0104;Name=BBa_B0104;Parent=BBa_S00164
BBa_I2038	.	sequence_feature	171	176	.	+	0	ID=annotation1920669;Name=AvrII/XbaI mixed site;Parent=BBa_B0104
BBa_I2038	.	ribosome_entry_site	177	196	.	+	0	ID=BBa_B2040;Name=BBa_B2040;Parent=BBa_I20240
BBa_I2038	.	engineered_region	1122	2055	.	+	0	ID=BBa_E70202;Name=BBa_E70202
BBa_I2038	.	terminator	1927	2006	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E70202
BBa_I2038	.	stem_loop	1938	1981	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I2038	.	engineered_region	1927	2006	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I2038	.	CDS	1199	1918	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E70202
BBa_I2038	.	CDS	1199	1918	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_I2038	.	terminator	2015	2055	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E70202
BBa_I2038	.	polyA_site	2042	2055	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I2038	.	stem_loop	2022	2041	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I2038	.	engineered_region	2015	2055	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I2038	.	stop_codon	2048	2048	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I2038	.	promoter	1122	1175	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_E70202
BBa_I2038	.	non_covalent_binding_site	1122	1140	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I2038	.	promoter	1164	1169	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I2038	.	promoter	1141	1146	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I2038	.	non_covalent_binding_site	1147	1165	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I2038	.	engineered_region	1122	1175	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I2038	.	ribosome_entry_site	1184	1192	.	+	0	ID=BBa_B0073;Name=BBa_B0073;Parent=BBa_E70202
>BBa_I2038
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacagccaggggtgctcggcataagccg
aagatatcggtagagttaatattgagcagatcccccggtgaaggatttaaccgtgttatctcgttggagatattcatgg
cgtattttggatcctagaaagacatggagacacatttaatggtgagcaagggcgaggaggataacatggccatcatcaa
ggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggc
cgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgt
cccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccc
cgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcag
gacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagacca
tgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagct
gaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctac
aacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggcc
gccactccaccggcggcatggacgagctgtacaagtaataatactagaggatcgcctgatcccggtgcacccgggcagc
tgcatagtctgggtgcaccgggatcaggtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggc
ctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgc
gtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtc
acaccactactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgtt
aatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgca
ctactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagataccc
agatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaa
gatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggta
ttgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggc
agacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagac
cattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccc
tttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcat
ggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcg
ttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
a