LOCUS BBa_I2044 3868 bp DNA linear 25-Nov-2024 DEFINITION Double protein generator (T7 RNAP and LacI) ACCESSION BBa_I2044 VERSION BBa_I2044.1 FEATURES Location/Qualifiers RBS 1..13 /label=RBS-3\Weak RBS 7..10 /label=RBS misc_feature 1..13 /label=BBa_B0032 RBS 1..13 /label=BBa_B0032 promoter 2517..2544 /label=putative E. coli promoter CDS 20..2674 /label=BBa_I2032 misc_feature 1..2674 /label=BBa_S03703 RBS 2683..2695 /label=RBS-3\Weak RBS 2689..2692 /label=RBS misc_feature 2683..2695 /label=BBa_B0032 RBS 2683..2695 /label=BBa_B0032 misc_feature 3788..3812 /label=Help:Barcodes CDS 2702..3812 /label=BBa_C0013 terminator 3821..3868 /label=BBa_B0016 misc_feature 2683..3868 /label=BBa_I2034 ORIGIN 1 tcacacagga aagtactaga tgaacacgat taacatcgct aagaacgact tctctgacat 61 cgaactggct gctatcccgt tcaacactct ggctgaccat tacggtgagc gtttagctcg 121 cgaacagttg gcccttgagc atgagtctta cgagatgggt gaagcacgct tccgcaagat 181 gtttgagcgt caacttaaag ctggtgaggt tgcggataac gctgccgcca agcctctcat 241 cactacccta ctccctaaga tgattgcacg catcaacgac tggtttgagg aagtgaaagc 301 taagcgcggc aagcgcccga cagccttcca gttcctgcaa gaaatcaagc cggaagccgt 361 agcgtacatc accattaaga ccactctggc ttgcctaacc agtgctgaca atacaaccgt 421 tcaggctgta gcaagcgcaa tcggtcgggc cattgaggac gaggctcgct tcggtcgtat 481 ccgtgacctt gaagctaagc acttcaagaa aaacgttgag gaacaactca acaagcgcgt 541 agggcacgtc tacaagaaag catttatgca agttgtcgag gctgacatgc tctctaaggg 601 tctactcggt ggcgaggcgt ggtcttcgtg gcataaggaa gactctattc atgtaggagt 661 acgctgcatc gagatgctca ttgagtcaac cggaatggtt agcttacacc gccaaaatgc 721 tggcgtagta ggtcaagact ctgagactat cgaactcgca cctgaatacg ctgaggctat 781 cgcaacccgt gcaggtgcgc tggctggcat ctctccgatg ttccaacctt gcgtagttcc 841 tcctaagccg tggactggca ttactggtgg tggctattgg gctaacggtc gtcgtcctct 901 ggcgctggtg cgtactcaca gtaagaaagc actgatgcgc tacgaagacg tttacatgcc 961 tgaggtgtac aaagcgatta acattgcgca aaacaccgca tggaaaatca acaagaaagt 1021 cctagcggtc gccaacgtaa tcaccaagtg gaagcattgt ccggtcgagg acatccctgc 1081 gattgagcgt gaagaactcc cgatgaaacc ggaagacatc gacatgaatc ctgaggctct 1141 caccgcgtgg aaacgtgctg ccgctgctgt gtaccgcaag gacaaggctc gcaagtctcg 1201 ccgtatcagc cttgagttca tgcttgagca agccaataag tttgctaacc ataaggccat 1261 ctggttccct tacaacatgg actggcgcgg tcgtgtttac gctgtgtcaa tgttcaaccc 1321 gcaaggtaac gatatgacca aaggactgct tacgctggcg aaaggtaaac caatcggtaa 1381 ggaaggttac tactggctga aaatccacgg tgcaaactgt gcgggtgtcg ataaggttcc 1441 gttccctgag cgcatcaagt tcattgagga aaaccacgag aacatcatgg cttgcgctaa 1501 gtctccactg gagaacactt ggtgggctga gcaagattct ccgttctgct tccttgcgtt 1561 ctgctttgag tacgctgggg tacagcacca cggcctgagc tataactgct cccttccgct 1621 ggcgtttgac gggtcttgct ctggcatcca gcacttctcc gcgatgctcc gagatgaggt 1681 aggtggtcgc gcggttaact tgcttcctag tgaaaccgtt caggacatct acgggattgt 1741 tgctaagaaa gtcaacgaga ttctacaagc agacgcaatc aatgggaccg ataacgaagt 1801 agttaccgtg accgatgaga acactggtga aatctctgag aaagtcaagc tgggcactaa 1861 ggcactggct ggtcaatggc tggcttacgg tgttactcgc agtgtgacta agcgttcagt 1921 catgacgctg gcttacgggt ccaaagagtt cggcttccgt caacaagtgc tggaagatac 1981 cattcagcca gctattgatt ccggcaaggg tctgatgttc actcagccga atcaggctgc 2041 tggatacatg gctaagctga tttgggaatc tgtgagcgtg acggtggtag ctgcggttga 2101 agcaatgaac tggcttaagt ctgctgctaa gctgctggct gctgaggtca aagataagaa 2161 gactggagag attcttcgca agcgttgcgc tgtgcattgg gtaactcctg atggtttccc 2221 tgtgtggcag gaatacaaga agcctattca gacgcgcttg aacctgatgt tcctcggtca 2281 gttccgctta cagcctacca ttaacaccaa caaagatagc gagattgatg cacacaaaca 2341 ggagtctggt atcgctccta actttgtaca cagccaagac ggtagccacc ttcgtaagac 2401 tgtagtgtgg gcacacgaga agtacggaat cgaatctttt gcactgattc acgactcctt 2461 cggtaccatt ccggctgacg ctgcgaacct gttcaaagca gtgcgcgaaa ctatggttga 2521 cacatatgag tcttgtgatg tactggctga tttctacgac cagttcgctg accagttgca 2581 cgagtctcaa ttggacaaaa tgccagcact tccggctaaa ggtaacttga acctccgtga 2641 catcttagag tcggacttcg cgttcgcgta ataatactag agtcacacag gaaagtacta 2701 gatgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt 2761 ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc 2821 ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca 2881 gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt 2941 cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga 3001 acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag 3061 tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg 3121 cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat 3181 tttctcccat gaagacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca 3241 gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg 3301 ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg 3361 gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca tcgttcccac 3421 tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc 3481 cgggctgcgc gttggtgcgg atatctcggt agtgggatac gacgataccg aagacagctc 3541 atgttatatc ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag 3601 cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc 3661 cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg 3721 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca 3781 gtaataacac tgatagtgct agtgtagatc actactagag ctagcataac cccttggggc 3841 ctctaaacgg gtcttgaggg gttttttg //