LOCUS       BBa_I2045               1943 bp    DNA     linear       22-Dec-2024
DEFINITION  mCherry & GFP expression device for testing a biological virtual 
            machine
ACCESSION   BBa_I2045
VERSION     BBa_I2045.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     stem_loop       56..118
                     /label=hp1
     misc_feature    119..129
                     /label=ss1
     stem_loop       130..158
                     /label=hp2
     misc_feature    159..170
                     /label=ss2
     misc_feature    56..170
                     /label=BBa_B0100
     misc_feature    171..176
                     /label=AvrII/XbaI mixed site
     misc_feature    171..176
                     /label=BBa_B0104
     misc_feature    1..176
                     /label=BBa_S00164
     RBS             177..196
                     /label=BBa_B2022
     CDS             197..907
                     /label=mCherry
     misc_feature    548..548
                     /label=C->T (removing PstI site)
     misc_feature    197..910
                     /label=BBa_J06504
     stem_loop       924..976
                     /label=stem_loop
     misc_feature    919..976
                     /label=BBa_B0101
     stem_loop       996..1039
                     /label=stem_loop
     misc_feature    985..1064
                     /label=BBa_B0010
     terminator      985..1064
                     /label=BBa_B0010
     stem_loop       1080..1099
                     /label=T7 TE
     misc_feature    1106..1106
                     /label=stop
     polyA_site      1100..1113
                     /label=polya
     misc_feature    1073..1113
                     /label=BBa_B0012
     terminator      1073..1113
                     /label=BBa_B0012
     misc_feature    197..1113
                     /label=BBa_S00162
     misc_feature    1..1113
                     /label=BBa_I20222
     promoter        1122..1144
                     /label=BBa_R0183
     RBS             1153..1161
                     /label=BBa_B0073
     CDS             1168..1887
                     /label=GFP protein
     CDS             1168..1887
                     /label=BBa_E0040
     terminator      1896..1943
                     /label=BBa_B0016
     misc_feature    1122..1943
                     /label=BBa_E71205
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacagccag
       61 gggtgctcgg cataagccga agatatcggt agagttaata ttgagcagat cccccggtga
      121 aggatttaac cgtgttatct cgttggagat attcatggcg tattttggat cctagaaacc
      181 ctcaggaggt aaaccaatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga
      241 gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga
      301 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
      361 gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa
      421 ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg
      481 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga
      541 ctcctccttg caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc
      601 ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat
      661 gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg
      721 cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc
      781 cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat
      841 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta
      901 caagtaataa tactagagga tcgcctgatc ccggtgcacc cgggcagctg catagtctgg
      961 gtgcaccggg atcaggtact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
     1021 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     1081 gctcaccttc gggtgggcct ttctgcgttt atatactaga gtcatacgac tcactatagg
     1141 gagatactag agtcacacca ctactagatg cgtaaaggag aagaactttt cactggagtt
     1201 gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga
     1261 gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga
     1321 aaactacctg ttccatggcc aacacttgtc actactttcg gttatggtgt tcaatgcttt
     1381 gcgagatacc cagatcatat gaaacagcat gactttttca agagtgccat gcccgaaggt
     1441 tatgtacagg aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa
     1501 gtcaagtttg aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa
     1561 gaagatggaa acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac
     1621 atcatggcag acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt
     1681 gaagatggaa gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc
     1741 cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc
     1801 aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat
     1861 ggcatggatg aactatacaa ataataatac tagagctagc ataacccctt ggggcctcta
     1921 aacgggtctt gaggggtttt ttg
//