LOCUS BBa_I2048 3920 bp DNA linear 20-Nov-2024 DEFINITION Positive and negative feedback regulated T7 RNAP expression system ACCESSION BBa_I2048 VERSION BBa_I2048.1 FEATURES Location/Qualifiers promoter 1..20 /label=BBa_R0183 fragment misc_feature 20..44 /label=lacI binding site misc_feature 31..31 /label=Center of binding site symmetry promoter 1..44 /label=BBa_R0184 RBS 53..65 /label=RBS-3\Weak RBS 59..62 /label=RBS misc_feature 53..65 /label=BBa_B0032 RBS 53..65 /label=BBa_B0032 promoter 2569..2596 /label=putative E. coli promoter CDS 72..2726 /label=BBa_I2032 misc_feature 53..2726 /label=BBa_S03703 RBS 2735..2747 /label=RBS-3\Weak RBS 2741..2744 /label=RBS misc_feature 2735..2747 /label=BBa_B0032 RBS 2735..2747 /label=BBa_B0032 misc_feature 3840..3864 /label=Help:Barcodes CDS 2754..3864 /label=BBa_C0013 terminator 3873..3920 /label=BBa_B0016 misc_feature 2735..3920 /label=BBa_I2034 misc_feature 53..3920 /label=BBa_I2044 ORIGIN 1 tcatacgact cactataggg gaattgtgag cggataacaa ttcctactag agtcacacag 61 gaaagtacta gatgaacacg attaacatcg ctaagaacga cttctctgac atcgaactgg 121 ctgctatccc gttcaacact ctggctgacc attacggtga gcgtttagct cgcgaacagt 181 tggcccttga gcatgagtct tacgagatgg gtgaagcacg cttccgcaag atgtttgagc 241 gtcaacttaa agctggtgag gttgcggata acgctgccgc caagcctctc atcactaccc 301 tactccctaa gatgattgca cgcatcaacg actggtttga ggaagtgaaa gctaagcgcg 361 gcaagcgccc gacagccttc cagttcctgc aagaaatcaa gccggaagcc gtagcgtaca 421 tcaccattaa gaccactctg gcttgcctaa ccagtgctga caatacaacc gttcaggctg 481 tagcaagcgc aatcggtcgg gccattgagg acgaggctcg cttcggtcgt atccgtgacc 541 ttgaagctaa gcacttcaag aaaaacgttg aggaacaact caacaagcgc gtagggcacg 601 tctacaagaa agcatttatg caagttgtcg aggctgacat gctctctaag ggtctactcg 661 gtggcgaggc gtggtcttcg tggcataagg aagactctat tcatgtagga gtacgctgca 721 tcgagatgct cattgagtca accggaatgg ttagcttaca ccgccaaaat gctggcgtag 781 taggtcaaga ctctgagact atcgaactcg cacctgaata cgctgaggct atcgcaaccc 841 gtgcaggtgc gctggctggc atctctccga tgttccaacc ttgcgtagtt cctcctaagc 901 cgtggactgg cattactggt ggtggctatt gggctaacgg tcgtcgtcct ctggcgctgg 961 tgcgtactca cagtaagaaa gcactgatgc gctacgaaga cgtttacatg cctgaggtgt 1021 acaaagcgat taacattgcg caaaacaccg catggaaaat caacaagaaa gtcctagcgg 1081 tcgccaacgt aatcaccaag tggaagcatt gtccggtcga ggacatccct gcgattgagc 1141 gtgaagaact cccgatgaaa ccggaagaca tcgacatgaa tcctgaggct ctcaccgcgt 1201 ggaaacgtgc tgccgctgct gtgtaccgca aggacaaggc tcgcaagtct cgccgtatca 1261 gccttgagtt catgcttgag caagccaata agtttgctaa ccataaggcc atctggttcc 1321 cttacaacat ggactggcgc ggtcgtgttt acgctgtgtc aatgttcaac ccgcaaggta 1381 acgatatgac caaaggactg cttacgctgg cgaaaggtaa accaatcggt aaggaaggtt 1441 actactggct gaaaatccac ggtgcaaact gtgcgggtgt cgataaggtt ccgttccctg 1501 agcgcatcaa gttcattgag gaaaaccacg agaacatcat ggcttgcgct aagtctccac 1561 tggagaacac ttggtgggct gagcaagatt ctccgttctg cttccttgcg ttctgctttg 1621 agtacgctgg ggtacagcac cacggcctga gctataactg ctcccttccg ctggcgtttg 1681 acgggtcttg ctctggcatc cagcacttct ccgcgatgct ccgagatgag gtaggtggtc 1741 gcgcggttaa cttgcttcct agtgaaaccg ttcaggacat ctacgggatt gttgctaaga 1801 aagtcaacga gattctacaa gcagacgcaa tcaatgggac cgataacgaa gtagttaccg 1861 tgaccgatga gaacactggt gaaatctctg agaaagtcaa gctgggcact aaggcactgg 1921 ctggtcaatg gctggcttac ggtgttactc gcagtgtgac taagcgttca gtcatgacgc 1981 tggcttacgg gtccaaagag ttcggcttcc gtcaacaagt gctggaagat accattcagc 2041 cagctattga ttccggcaag ggtctgatgt tcactcagcc gaatcaggct gctggataca 2101 tggctaagct gatttgggaa tctgtgagcg tgacggtggt agctgcggtt gaagcaatga 2161 actggcttaa gtctgctgct aagctgctgg ctgctgaggt caaagataag aagactggag 2221 agattcttcg caagcgttgc gctgtgcatt gggtaactcc tgatggtttc cctgtgtggc 2281 aggaatacaa gaagcctatt cagacgcgct tgaacctgat gttcctcggt cagttccgct 2341 tacagcctac cattaacacc aacaaagata gcgagattga tgcacacaaa caggagtctg 2401 gtatcgctcc taactttgta cacagccaag acggtagcca ccttcgtaag actgtagtgt 2461 gggcacacga gaagtacgga atcgaatctt ttgcactgat tcacgactcc ttcggtacca 2521 ttccggctga cgctgcgaac ctgttcaaag cagtgcgcga aactatggtt gacacatatg 2581 agtcttgtga tgtactggct gatttctacg accagttcgc tgaccagttg cacgagtctc 2641 aattggacaa aatgccagca cttccggcta aaggtaactt gaacctccgt gacatcttag 2701 agtcggactt cgcgttcgcg taataatact agagtcacac aggaaagtac tagatgaaac 2761 cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg 2821 tggtgaacca ggccagccac gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg 2881 cggagctgaa ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc 2941 tgattggcgt tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga 3001 ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg 3061 gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga 3121 tcattaacta tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg 3181 ttccggcgtt atttcttgat gtctctgacc agacacccat caacagtatt attttctccc 3241 atgaagacgg tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg 3301 cgctgttagc gggcccatta agttctgtct cggcgcgtct gcgtctggct ggctggcata 3361 aatatctcac tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca 3421 tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc 3481 tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc 3541 gcgttggtgc ggatatctcg gtagtgggat acgacgatac cgaagacagc tcatgttata 3601 tcccgccgtt aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc 3661 gcttgctgca actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac 3721 tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg 3781 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtaataac 3841 actgatagtg ctagtgtaga tcactactag agctagcata accccttggg gcctctaaac 3901 gggtcttgag gggttttttg //