BBa_I2048
type: ComponentDefinition
persistent identity: BBa I2048
display id: BBa_I2048
version: 1
was generated by: iGEM to SBOL conversion
description: Positive and negative feedback regulated T7 RNAP expression system
created: 2007-06-07T11:00:00Z
modified: 2015-08-31T04:07:40Z
mutable provenance: Based on and developed from a system built by Bill Studier at Brookhaven National Labs
owned by: james
owned by: myers
mutable description: This part is an intermediate version of a biological virtual machine. T7 RNAP expression is controlled by a T7 promoter, forming a positive feedback loop or autogene. LacI expression is controlled by the same promoter. The promoter is repressible by LacI leading to negative feedback. The combination of positive and negative feedback should lead to a moderate, stable level of T7 RNAP in the cell.
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: n/a
star: false
creator: Bartholomew Canton
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_I2044
component: BBa_R0184
sequence annotation: BBa_R0184
sequence annotation: BBa_I2044
sequence: https://synbiohub.org/public/igem/BBa_I2048_sequence/1

BBa_I2044
type: Component
persistent identity: component2260755
display id: component2260755
version: 1
top level: BBa_I2048
definition: BBa_I2044
access: public

BBa_R0184
type: Component
persistent identity: component2260741
display id: component2260741
version: 1
top level: BBa_I2048
definition: BBa_R0184
access: public

BBa_R0184
type: SequenceAnnotation
persistent identity: annotation2260741
display id: annotation2260741
version: 1
top level: BBa_I2048
location: https://synbiohub.org/public/igem/BBa_I2048/annotation2260741/range2260741/1
component: BBa_R0184

https://synbiohub.org/public/igem/BBa_I2048/annotation2260741/range2260741/1
type: Range
persistent identity: range2260741
display id: range2260741
version: 1
top level: BBa_I2048
direction: direction/reverse
start: 1
end: 44
orientation: inline

BBa_I2044
type: SequenceAnnotation
persistent identity: annotation2260755
display id: annotation2260755
version: 1
top level: BBa_I2048
location: https://synbiohub.org/public/igem/BBa_I2048/annotation2260755/range2260755/1
component: BBa_I2044

https://synbiohub.org/public/igem/BBa_I2048/annotation2260755/range2260755/1
type: Range
persistent identity: range2260755
display id: range2260755
version: 1
top level: BBa_I2048
direction: direction/reverse
start: 53
end: 3920
orientation: inline

BBa_I2044
type: ComponentDefinition
persistent identity: BBa I2044
display id: BBa_I2044
version: 1
was generated by: iGEM to SBOL conversion
description: Double protein generator (T7 RNAP and LacI)
created: 2007-06-03T11:00:00Z
modified: 2015-08-31T04:07:40Z
mutable provenance: n/a
owned by: james
owned by: myers
mutable description: LacI and T7 RNAP are transcribed polycistronically and translation of both is regulated by B0032. A T7 terminator is downstream. This part is being used in the construction of VM2.0
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: n/a
star: false
creator: Bartholomew Canton
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I2034
component: BBa_S03703
sequence annotation: BBa_S03703
sequence annotation: BBa_I2034
sequence: https://synbiohub.org/public/igem/BBa_I2044_sequence/1

BBa_I2034
type: Component
persistent identity: component2252664
display id: component2252664
version: 1
top level: BBa_I2044
definition: BBa_I2034
access: public

BBa_S03703
type: Component
persistent identity: component2252657
display id: component2252657
version: 1
top level: BBa_I2044
definition: BBa_S03703
access: public

BBa_S03703
type: SequenceAnnotation
persistent identity: annotation2252657
display id: annotation2252657
version: 1
top level: BBa_I2044
location: https://synbiohub.org/public/igem/BBa_I2044/annotation2252657/range2252657/1
component: BBa_S03703

https://synbiohub.org/public/igem/BBa_I2044/annotation2252657/range2252657/1
type: Range
persistent identity: range2252657
display id: range2252657
version: 1
top level: BBa_I2044
direction: direction/reverse
start: 1
end: 2674
orientation: inline

BBa_I2034
type: SequenceAnnotation
persistent identity: annotation2252664
display id: annotation2252664
version: 1
top level: BBa_I2044
location: https://synbiohub.org/public/igem/BBa_I2044/annotation2252664/range2252664/1
component: BBa_I2034

https://synbiohub.org/public/igem/BBa_I2044/annotation2252664/range2252664/1
type: Range
persistent identity: range2252664
display id: range2252664
version: 1
top level: BBa_I2044
direction: direction/reverse
start: 2683
end: 3868
orientation: inline

BBa_R0184
type: ComponentDefinition
persistent identity: BBa R0184
display id: BBa_R0184
version: 1
was generated by: iGEM to SBOL conversion
description: T7 promoter (lacI repressible)
created: 2007-04-25T11:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: This sequence is based on the T7lac promoter described by Dubendorff and Studier (JMB 1991, 219, 45-59). The only modification is the mutation at -16.
owned by: james
owned by: myers
mutable description: A mutant version of the T7lac promoter designed by Dubendorff and Studier. The promoter is based on the strong ??10 T7 promoter but incorporates an A->C mutation at -16. The LacI operator site is a partially symmetric 25bp sequence.
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The -16 mutation was included to weaken the consensus promoter as described by Imburgio and co-workers (see BBa_R0183 for more information)
star: false
creator: Bartholomew Canton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0183 fragment
sequence annotation: lacI binding site
sequence annotation: Center of binding site symmetry
sequence: https://synbiohub.org/public/igem/BBa_R0184_sequence/1

BBa_R0183 fragment
type: SequenceAnnotation
persistent identity: annotation1932678
display id: annotation1932678
version: 1
top level: BBa_R0184
location: https://synbiohub.org/public/igem/BBa_R0184/annotation1932678/range1932678/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0184/annotation1932678/range1932678/1
type: Range
persistent identity: range1932678
display id: range1932678
version: 1
top level: BBa_R0184
direction: direction/forward
start: 1
end: 20
orientation: inline

lacI binding site
type: SequenceAnnotation
persistent identity: annotation1932679
display id: annotation1932679
version: 1
top level: BBa_R0184
location: https://synbiohub.org/public/igem/BBa_R0184/annotation1932679/range1932679/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0184/annotation1932679/range1932679/1
type: Range
persistent identity: range1932679
display id: range1932679
version: 1
top level: BBa_R0184
direction: direction/forward
start: 20
end: 44
orientation: inline

Center of binding site symmetry
type: SequenceAnnotation
persistent identity: annotation1932680
display id: annotation1932680
version: 1
top level: BBa_R0184
location: https://synbiohub.org/public/igem/BBa_R0184/annotation1932680/range1932680/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_R0184/annotation1932680/range1932680/1
type: Range
persistent identity: range1932680
display id: range1932680
version: 1
top level: BBa_R0184
direction: direction/forward
start: 31
end: 31
orientation: inline

BBa_S03703
type: ComponentDefinition
persistent identity: BBa S03703
display id: BBa_S03703
version: 1
was generated by: iGEM to SBOL conversion
description: B0032:I2032
created: 2007-05-14T11:00:00Z
modified: 2015-05-08T01:14:29Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _84_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Bartholomew Canton
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0032
component: BBa_I2032
sequence annotation: BBa_B0032
sequence annotation: BBa_I2032
sequence: https://synbiohub.org/public/igem/BBa_S03703_sequence/1

BBa_B0032
type: Component
persistent identity: component2218407
display id: component2218407
version: 1
top level: BBa_S03703
definition: BBa_B0032
access: public

BBa_I2032
type: Component
persistent identity: component2218410
display id: component2218410
version: 1
top level: BBa_S03703
definition: BBa_I2032
access: public

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2218407
display id: annotation2218407
version: 1
top level: BBa_S03703
location: https://synbiohub.org/public/igem/BBa_S03703/annotation2218407/range2218407/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_S03703/annotation2218407/range2218407/1
type: Range
persistent identity: range2218407
display id: range2218407
version: 1
top level: BBa_S03703
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_I2032
type: SequenceAnnotation
persistent identity: annotation2218410
display id: annotation2218410
version: 1
top level: BBa_S03703
location: https://synbiohub.org/public/igem/BBa_S03703/annotation2218410/range2218410/1
component: BBa_I2032

https://synbiohub.org/public/igem/BBa_S03703/annotation2218410/range2218410/1
type: Range
persistent identity: range2218410
display id: range2218410
version: 1
top level: BBa_S03703
direction: direction/reverse
start: 20
end: 2674
orientation: inline

BBa_I2034
type: ComponentDefinition
persistent identity: BBa I2034
display id: BBa_I2034
version: 1
was generated by: iGEM to SBOL conversion
description: LacI protein generator
created: 2007-05-17T11:00:00Z
modified: 2015-08-31T04:07:40Z
mutable provenance: n/a
owned by: james
owned by: myers
mutable description: This protein generator is composed of an E. coli RBS, a lacI coding region and a T7 terminator. It is designed for use with a T7 promoter but should be functional with a E. coli promoter also. Ultimately, it will be used in an early version of a virtual machine.
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: B0032 is a very reliable RBS and B0016 is the T7 terminator
star: false
creator: Bartholomew Canton
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_C0013
component: BBa_B0032
component: BBa_B0016
sequence annotation: BBa_B0032
sequence annotation: BBa_C0013
sequence annotation: BBa_B0016
sequence: https://synbiohub.org/public/igem/BBa_I2034_sequence/1

BBa_C0013
type: Component
persistent identity: component2245562
display id: component2245562
version: 1
top level: BBa_I2034
definition: lacI
access: public

BBa_B0032
type: Component
persistent identity: component2245559
display id: component2245559
version: 1
top level: BBa_I2034
definition: BBa_B0032
access: public

BBa_B0016
type: Component
persistent identity: component2245563
display id: component2245563
version: 1
top level: BBa_I2034
definition: BBa_B0016
access: public

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2245559
display id: annotation2245559
version: 1
top level: BBa_I2034
location: https://synbiohub.org/public/igem/BBa_I2034/annotation2245559/range2245559/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_I2034/annotation2245559/range2245559/1
type: Range
persistent identity: range2245559
display id: range2245559
version: 1
top level: BBa_I2034
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_C0013
type: SequenceAnnotation
persistent identity: annotation2245562
display id: annotation2245562
version: 1
top level: BBa_I2034
location: https://synbiohub.org/public/igem/BBa_I2034/annotation2245562/range2245562/1
component: BBa_C0013

https://synbiohub.org/public/igem/BBa_I2034/annotation2245562/range2245562/1
type: Range
persistent identity: range2245562
display id: range2245562
version: 1
top level: BBa_I2034
direction: direction/reverse
start: 20
end: 1130
orientation: inline

BBa_B0016
type: SequenceAnnotation
persistent identity: annotation2245563
display id: annotation2245563
version: 1
top level: BBa_I2034
location: https://synbiohub.org/public/igem/BBa_I2034/annotation2245563/range2245563/1
component: BBa_B0016

https://synbiohub.org/public/igem/BBa_I2034/annotation2245563/range2245563/1
type: Range
persistent identity: range2245563
display id: range2245563
version: 1
top level: BBa_I2034
direction: direction/reverse
start: 1139
end: 1186
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

lacI
type: ComponentDefinition
persistent identity: BBa C0013
display id: BBa_C0013
version: 1
was generated by: iGEM to SBOL conversion
description: Repressor, lacI
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: www.ncbi.nlm.nih.gov
owned by: james
owned by: myers
mutable description: LacI repressor protein, without SsrA degradation tag. No Barcode.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Yong-Su Jin (Fighting Darwins)
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0013_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214005
display id: annotation2214005
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0013/annotation2214005/range2214005/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0013/annotation2214005/range2214005/1
type: Range
persistent identity: range2214005
display id: range2214005
version: 1
top level: lacI
direction: direction/forward
start: 1087
end: 1111
orientation: inline

BBa_I2032
type: ComponentDefinition
persistent identity: BBa I2032
display id: BBa_I2032
version: 1
was generated by: iGEM to SBOL conversion
description: T7 RNA polymerase
created: 2007-04-11T11:00:00Z
modified: 2016-10-14T06:52:19Z
mutable provenance: Bacteriophage T7
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Gene 1 coding region from bacteriophage T7 encoding a RNAP polymerase (RNAP). This single-protein RNAP recognizes a 21 bp consensus promoter sequence and is orthogonal to E. coli RNAP.
discontinued: false
dominant: true
experience: experience/None
group u list: _11_
m user id: 27399
owner id: 135
owning group id: 84
sample status: In stock
status: status/Available
bookmark: false
mutable notes: n/a
star: false
creator: Bartholomew Canton
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: putative E. coli promoter
sequence: https://synbiohub.org/public/igem/BBa_I2032_sequence/1

putative E. coli promoter
type: SequenceAnnotation
persistent identity: annotation1928674
display id: annotation1928674
version: 1
top level: BBa_I2032
location: https://synbiohub.org/public/igem/BBa_I2032/annotation1928674/range1928674/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I2032/annotation1928674/range1928674/1
type: Range
persistent identity: range1928674
display id: range1928674
version: 1
top level: BBa_I2032
direction: direction/forward
start: 2498
end: 2525
orientation: inline

BBa_B0016
type: ComponentDefinition
persistent identity: BBa B0016
display id: BBa_B0016
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (T7 RNAP specific, T_Phi)
created: 2003-12-04T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Sri Kosuri
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: T7 gene 1 specific transcriptional terminator
discontinued: false
dominant: true
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Sri Kosuri
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_B0016_sequence/1

https://synbiohub.org/public/igem/BBa_I2048_sequence/1
type: Sequence
persistent identity: BBa I2048 sequence
display id: BBa_I2048_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcatacgactcactataggggaattgtgagcggataacaattcctactagagtcacacaggaaagtactagatgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctggcttacgggtccaaagag…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I2044_sequence/1
type: Sequence
persistent identity: BBa I2044 sequence
display id: BBa_I2044_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctggcttacgggtccaaagagttcggcttccgtcaacaagtgctggaagataccattcagccagctattgatt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0184_sequence/1
type: Sequence
persistent identity: BBa R0184 sequence
display id: BBa_R0184_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcatacgactcactataggggaattgtgagcggataacaattcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S03703_sequence/1
type: Sequence
persistent identity: BBa S03703 sequence
display id: BBa_S03703_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctggcttacgggtccaaagagttcggcttccgtcaacaagtgctggaagataccattcagccagctattgatt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I2034_sequence/1
type: Sequence
persistent identity: BBa I2034 sequence
display id: BBa_I2034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataacactgatagtgctagtgtagatcactactagagctagcataaccccttggggcctctaaacgggtcttgaggggttttttg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0013_sequence/1
type: Sequence
persistent identity: BBa C0013 sequence
display id: BBa_C0013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I2032_sequence/1
type: Sequence
persistent identity: BBa I2032 sequence
display id: BBa_I2032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctggcttacgggtccaaagagttcggcttccgtcaacaagtgctggaagataccattcagccagctattgattccggcaagggtctgatgtt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0016_sequence/1
type: Sequence
persistent identity: BBa B0016 sequence
display id: BBa_B0016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctagcataaccccttggggcctctaaacgggtcttgaggggttttttg
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z