LOCUS       BBa_I2050               2059 bp    DNA     linear       20-Nov-2024
DEFINITION  mCherry and GFP expression system
ACCESSION   BBa_I2050
VERSION     BBa_I2050.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     stem_loop       56..118
                     /label=hp1
     misc_feature    119..129
                     /label=ss1
     stem_loop       130..158
                     /label=hp2
     misc_feature    159..170
                     /label=ss2
     misc_feature    56..170
                     /label=BBa_B0100
     misc_feature    171..176
                     /label=AvrII/XbaI mixed site
     misc_feature    171..176
                     /label=BBa_B0104
     misc_feature    1..176
                     /label=BBa_S00164
     RBS             177..196
                     /label=BBa_B2040
     CDS             197..907
                     /label=mCherry
     misc_feature    548..548
                     /label=C->T (removing PstI site)
     misc_feature    197..910
                     /label=BBa_J06504
     stem_loop       924..976
                     /label=stem_loop
     misc_feature    919..976
                     /label=BBa_B0101
     stem_loop       996..1039
                     /label=stem_loop
     misc_feature    985..1064
                     /label=BBa_B0010
     terminator      985..1064
                     /label=BBa_B0010
     stem_loop       1080..1099
                     /label=T7 TE
     misc_feature    1106..1106
                     /label=stop
     polyA_site      1100..1113
                     /label=polya
     misc_feature    1073..1113
                     /label=BBa_B0012
     terminator      1073..1113
                     /label=BBa_B0012
     misc_feature    197..1113
                     /label=BBa_S00162
     misc_feature    1..1113
                     /label=BBa_I20240
     misc_feature    1122..1140
                     /label=TetR 1
     misc_feature    1122..1175
                     /label=BBa_R0040
     promoter        1141..1146
                     /label=-35
     misc_feature    1147..1165
                     /label=TetR 2
     promoter        1164..1169
                     /label=-10
     promoter        1122..1175
                     /label=BBa_R0040
     RBS             1184..1196
                     /label=RBS-3\Weak
     RBS             1190..1193
                     /label=RBS
     misc_feature    1184..1196
                     /label=BBa_B0032
     RBS             1184..1196
                     /label=BBa_B0032
     CDS             1203..1922
                     /label=GFP protein
     CDS             1203..1922
                     /label=BBa_E0040
     stem_loop       1942..1985
                     /label=stem_loop
     misc_feature    1931..2010
                     /label=BBa_B0010
     terminator      1931..2010
                     /label=BBa_B0010
     stem_loop       2026..2045
                     /label=T7 TE
     misc_feature    2052..2052
                     /label=stop
     polyA_site      2046..2059
                     /label=polya
     misc_feature    2019..2059
                     /label=BBa_B0012
     terminator      2019..2059
                     /label=BBa_B0012
     misc_feature    1122..2059
                     /label=BBa_I7101
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacagccag
       61 gggtgctcgg cataagccga agatatcggt agagttaata ttgagcagat cccccggtga
      121 aggatttaac cgtgttatct cgttggagat attcatggcg tattttggat cctagaaaga
      181 catggagaca catttaatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga
      241 gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga
      301 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
      361 gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa
      421 ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg
      481 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga
      541 ctcctccttg caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc
      601 ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat
      661 gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg
      721 cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc
      781 cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat
      841 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta
      901 caagtaataa tactagagga tcgcctgatc ccggtgcacc cgggcagctg catagtctgg
      961 gtgcaccggg atcaggtact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
     1021 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     1081 gctcaccttc gggtgggcct ttctgcgttt atatactaga gtccctatca gtgatagaga
     1141 ttgacatccc tatcagtgat agagatactg agcactacta gagtcacaca ggaaagtact
     1201 agatgcgtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg
     1261 gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg
     1321 gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac
     1381 ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat catatgaaac
     1441 agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt
     1501 tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg
     1561 ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca
     1621 aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg
     1681 gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt caactagcag
     1741 accattatca acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt
     1801 acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac cacatggtcc
     1861 ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta tacaaataat
     1921 aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     1981 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
     2041 gggcctttct gcgtttata
//