##gff-version 3
##sequence-region BBa_I2052 1 1973
BBa_I2052	.	ribosome_entry_site	1183	1191	.	+	0	ID=BBa_B0073;Name=BBa_B0073
BBa_I2052	.	mature_transcript_region	64	178	.	+	0	ID=BBa_B0100;Name=BBa_B0100
BBa_I2052	.	stem_loop	64	126	.	+	0	ID=annotation1919681;Name=hp1;Parent=BBa_B0100
BBa_I2052	.	sequence_feature	167	178	.	+	0	ID=annotation1919684;Name=ss2;Parent=BBa_B0100
BBa_I2052	.	sequence_feature	127	137	.	+	0	ID=annotation1919682;Name=ss1;Parent=BBa_B0100
BBa_I2052	.	stem_loop	138	166	.	+	0	ID=annotation1919683;Name=hp2;Parent=BBa_B0100
BBa_I2052	.	terminator	1926	1973	.	+	0	ID=BBa_B0016;Name=BBa_B0016
BBa_I2052	.	terminator	1103	1143	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I2052	.	engineered_region	1103	1143	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I2052	.	stop_codon	1136	1136	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I2052	.	polyA_site	1130	1143	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I2052	.	stem_loop	1110	1129	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I2052	.	mature_transcript_region	949	1006	.	+	0	ID=BBa_B0101;Name=BBa_B0101
BBa_I2052	.	stem_loop	954	1006	.	+	0	ID=annotation1919685;Name=stem_loop;Parent=BBa_B0101
BBa_I2052	.	sequence_feature	187	192	.	+	0	ID=BBa_B0104;Name=BBa_B0104
BBa_I2052	.	sequence_feature	187	192	.	+	0	ID=annotation1920669;Name=AvrII/XbaI mixed site;Parent=BBa_B0104
BBa_I2052	.	engineered_region	227	940	.	+	0	ID=BBa_J06504;Name=BBa_J06504
BBa_I2052	.	sequence_alteration	578	578	.	+	0	ID=annotation1585833;Name=C->T (removing PstI site);Parent=BBa_J06504
BBa_I2052	.	CDS	227	937	.	+	0	ID=annotation1585829;Name=mCherry;Parent=BBa_J06504
BBa_I2052	.	CDS	1198	1917	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_I2052	.	CDS	1198	1917	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_I2052	.	promoter	1152	1174	.	+	0	ID=BBa_R0183;Name=BBa_R0183
BBa_I2052	.	terminator	1015	1094	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I2052	.	stem_loop	1026	1069	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I2052	.	engineered_region	1015	1094	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I2052	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_I2052	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_I2052	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_I2052	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_I2052	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_I2052	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_I2052	.	ribosome_entry_site	201	220	.	+	0	ID=BBa_B2022;Name=BBa_B2022
>BBa_I2052
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagaggccaggggtgctcggc
ataagccgaagatatcggtagagttaatattgagcagatcccccggtgaaggatttaaccgtgttatctcgttggagat
attcatggcgtattttggattactagagcctagatactagagaaccctcaggaggtaaaccatactagatggtgagcaa
gggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccac
gagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtg
gccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccga
catccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtg
gtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccct
ccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccct
gaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggcc
aagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacacca
tcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataatactagag
gatcgcctgatcccggtgcacccgggcagctgcatagtctgggtgcaccgggatcaggtactagagccaggcatcaaat
aaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcac
actggctcaccttcgggtgggcctttctgcgtttatatactagagtcatacgactcactatagggagatactagagtca
caccactactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgtta
atgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcac
tactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagataccca
gatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaag
atgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtat
tgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggca
gacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagacc
attatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccct
ttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatg
gatgaactatacaaataataatactagagctagcataaccccttggggcctctaaacgggtcttgaggggttttttg