BBa_I2052
type: ComponentDefinition
persistent identity: BBa I2052
display id: BBa_I2052
version: 1
was generated by: iGEM to SBOL conversion
description: mCherry and GFP expression system
created: 2007-06-14T11:00:00Z
modified: 2015-08-31T04:07:40Z
mutable provenance: n/a
owned by: james
owned by: myers
mutable description: This system includes two fluorescent protein generators. An mCherry generating device is controlled by a LacI-repressible promoter and a GFP generating device is controlled by a T7 promoter and an orthogonal ribosome binding site.
discontinued: true
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 135
owning group id: 84
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: n/a
star: false
creator: Bartholomew Canton
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0011
component: BBa_E0040
component: BBa_B0073
component: BBa_B0016
component: BBa_B0104
component: BBa_B0100
component: BBa_J06504
component: BBa_B2022
component: BBa_B0010
component: BBa_B0101
component: BBa_R0183
component: BBa_B0012
sequence annotation: BBa_R0011
sequence annotation: BBa_B0100
sequence annotation: BBa_B0104
sequence annotation: BBa_B2022
sequence annotation: BBa_J06504
sequence annotation: BBa_B0101
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0183
sequence annotation: BBa_B0073
sequence annotation: BBa_E0040
sequence annotation: BBa_B0016
sequence: https://synbiohub.org/public/igem/BBa_I2052_sequence/1

BBa_R0011
type: Component
persistent identity: component1934782
display id: component1934782
version: 1
top level: BBa_I2052
definition: lacI+pL
access: public

BBa_E0040
type: Component
persistent identity: component1934809
display id: component1934809
version: 1
top level: BBa_I2052
definition: GFP
access: public

BBa_B0073
type: Component
persistent identity: component1934807
display id: component1934807
version: 1
top level: BBa_I2052
definition: BBa_B0073
access: public

BBa_B0016
type: Component
persistent identity: component1934810
display id: component1934810
version: 1
top level: BBa_I2052
definition: BBa_B0016
access: public

BBa_B0104
type: Component
persistent identity: component1934793
display id: component1934793
version: 1
top level: BBa_I2052
definition: AvrII/XbaI
access: public

BBa_B0100
type: Component
persistent identity: component1934791
display id: component1934791
version: 1
top level: BBa_I2052
definition: OmpA 5
access: public

BBa_J06504
type: Component
persistent identity: component1934797
display id: component1934797
version: 1
top level: BBa_I2052
definition: mCherry
access: public

BBa_B2022
type: Component
persistent identity: component1934794
display id: component1934794
version: 1
top level: BBa_I2052
definition: BBa_B2022
access: public

BBa_B0010
type: Component
persistent identity: component1934800
display id: component1934800
version: 1
top level: BBa_I2052
definition: BBa_B0010
access: public

BBa_B0101
type: Component
persistent identity: component1934799
display id: component1934799
version: 1
top level: BBa_I2052
definition: HP3 3
access: public

BBa_R0183
type: Component
persistent identity: component1934806
display id: component1934806
version: 1
top level: BBa_I2052
definition: BBa_R0183
access: public

BBa_B0012
type: Component
persistent identity: component1934802
display id: component1934802
version: 1
top level: BBa_I2052
definition: BBa_B0012
access: public

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1934782
display id: annotation1934782
version: 1
top level: BBa_I2052
location: https://synbiohub.org/public/igem/BBa_I2052/annotation1934782/range1934782/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_I2052/annotation1934782/range1934782/1
type: Range
persistent identity: range1934782
display id: range1934782
version: 1
top level: BBa_I2052
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0100
type: SequenceAnnotation
persistent identity: annotation1934791
display id: annotation1934791
version: 1
top level: BBa_I2052
location: https://synbiohub.org/public/igem/BBa_I2052/annotation1934791/range1934791/1
component: BBa_B0100

https://synbiohub.org/public/igem/BBa_I2052/annotation1934791/range1934791/1
type: Range
persistent identity: range1934791
display id: range1934791
version: 1
top level: BBa_I2052
direction: direction/reverse
start: 64
end: 178
orientation: inline

BBa_B0104
type: SequenceAnnotation
persistent identity: annotation1934793
display id: annotation1934793
version: 1
top level: BBa_I2052
location: https://synbiohub.org/public/igem/BBa_I2052/annotation1934793/range1934793/1
component: BBa_B0104

https://synbiohub.org/public/igem/BBa_I2052/annotation1934793/range1934793/1
type: Range
persistent identity: range1934793
display id: range1934793
version: 1
top level: BBa_I2052
direction: direction/reverse
start: 187
end: 192
orientation: inline

BBa_B2022
type: SequenceAnnotation
persistent identity: annotation1934794
display id: annotation1934794
version: 1
top level: BBa_I2052
location: https://synbiohub.org/public/igem/BBa_I2052/annotation1934794/range1934794/1
component: BBa_B2022

https://synbiohub.org/public/igem/BBa_I2052/annotation1934794/range1934794/1
type: Range
persistent identity: range1934794
display id: range1934794
version: 1
top level: BBa_I2052
direction: direction/reverse
start: 201
end: 220
orientation: inline

BBa_J06504
type: SequenceAnnotation
persistent identity: annotation1934797
display id: annotation1934797
version: 1
top level: BBa_I2052
location: https://synbiohub.org/public/igem/BBa_I2052/annotation1934797/range1934797/1
component: BBa_J06504

https://synbiohub.org/public/igem/BBa_I2052/annotation1934797/range1934797/1
type: Range
persistent identity: range1934797
display id: range1934797
version: 1
top level: BBa_I2052
direction: direction/reverse
start: 227
end: 940
orientation: inline

BBa_B0101
type: SequenceAnnotation
persistent identity: annotation1934799
display id: annotation1934799
version: 1
top level: BBa_I2052
location: https://synbiohub.org/public/igem/BBa_I2052/annotation1934799/range1934799/1
component: BBa_B0101

https://synbiohub.org/public/igem/BBa_I2052/annotation1934799/range1934799/1
type: Range
persistent identity: range1934799
display id: range1934799
version: 1
top level: BBa_I2052
direction: direction/reverse
start: 949
end: 1006
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1934800
display id: annotation1934800
version: 1
top level: BBa_I2052
location: https://synbiohub.org/public/igem/BBa_I2052/annotation1934800/range1934800/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I2052/annotation1934800/range1934800/1
type: Range
persistent identity: range1934800
display id: range1934800
version: 1
top level: BBa_I2052
direction: direction/forward
start: 1015
end: 1094
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1934802
display id: annotation1934802
version: 1
top level: BBa_I2052
location: https://synbiohub.org/public/igem/BBa_I2052/annotation1934802/range1934802/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I2052/annotation1934802/range1934802/1
type: Range
persistent identity: range1934802
display id: range1934802
version: 1
top level: BBa_I2052
direction: direction/forward
start: 1103
end: 1143
orientation: inline

BBa_R0183
type: SequenceAnnotation
persistent identity: annotation1934806
display id: annotation1934806
version: 1
top level: BBa_I2052
location: https://synbiohub.org/public/igem/BBa_I2052/annotation1934806/range1934806/1
component: BBa_R0183

https://synbiohub.org/public/igem/BBa_I2052/annotation1934806/range1934806/1
type: Range
persistent identity: range1934806
display id: range1934806
version: 1
top level: BBa_I2052
direction: direction/reverse
start: 1152
end: 1174
orientation: inline

BBa_B0073
type: SequenceAnnotation
persistent identity: annotation1934807
display id: annotation1934807
version: 1
top level: BBa_I2052
location: https://synbiohub.org/public/igem/BBa_I2052/annotation1934807/range1934807/1
component: BBa_B0073

https://synbiohub.org/public/igem/BBa_I2052/annotation1934807/range1934807/1
type: Range
persistent identity: range1934807
display id: range1934807
version: 1
top level: BBa_I2052
direction: direction/reverse
start: 1183
end: 1191
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1934809
display id: annotation1934809
version: 1
top level: BBa_I2052
location: https://synbiohub.org/public/igem/BBa_I2052/annotation1934809/range1934809/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I2052/annotation1934809/range1934809/1
type: Range
persistent identity: range1934809
display id: range1934809
version: 1
top level: BBa_I2052
direction: direction/reverse
start: 1198
end: 1917
orientation: inline

BBa_B0016
type: SequenceAnnotation
persistent identity: annotation1934810
display id: annotation1934810
version: 1
top level: BBa_I2052
location: https://synbiohub.org/public/igem/BBa_I2052/annotation1934810/range1934810/1
component: BBa_B0016

https://synbiohub.org/public/igem/BBa_I2052/annotation1934810/range1934810/1
type: Range
persistent identity: range1934810
display id: range1934810
version: 1
top level: BBa_I2052
direction: direction/reverse
start: 1926
end: 1973
orientation: inline

OmpA 5
type: ComponentDefinition
persistent identity: BBa B0100
display id: BBa_B0100
version: 1
was generated by: iGEM to SBOL conversion
description: OmpA 5
created: 2007-02-28T12:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: MG1655 genome, OmpA gene
owned by: james
owned by: myers
mutable description: 5 prime UTR from the OmpA gene in E.coli used for stabilizing the downstream RNA transcript
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 571
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This region was PCR amplified from the genome of MG1655.
star: false
creator: Heather Keller
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: hp1
sequence annotation: ss1
sequence annotation: hp2
sequence annotation: ss2
sequence: https://synbiohub.org/public/igem/BBa_B0100_sequence/1

hp1
type: SequenceAnnotation
persistent identity: annotation1919681
display id: annotation1919681
version: 1
top level: OmpA 5
location: https://synbiohub.org/public/igem/BBa_B0100/annotation1919681/range1919681/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0100/annotation1919681/range1919681/1
type: Range
persistent identity: range1919681
display id: range1919681
version: 1
top level: OmpA 5
direction: direction/forward
start: 1
end: 63
orientation: inline

ss1
type: SequenceAnnotation
persistent identity: annotation1919682
display id: annotation1919682
version: 1
top level: OmpA 5
location: https://synbiohub.org/public/igem/BBa_B0100/annotation1919682/range1919682/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_B0100/annotation1919682/range1919682/1
type: Range
persistent identity: range1919682
display id: range1919682
version: 1
top level: OmpA 5
direction: direction/forward
start: 64
end: 74
orientation: inline

hp2
type: SequenceAnnotation
persistent identity: annotation1919683
display id: annotation1919683
version: 1
top level: OmpA 5
location: https://synbiohub.org/public/igem/BBa_B0100/annotation1919683/range1919683/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0100/annotation1919683/range1919683/1
type: Range
persistent identity: range1919683
display id: range1919683
version: 1
top level: OmpA 5
direction: direction/forward
start: 75
end: 103
orientation: inline

ss2
type: SequenceAnnotation
persistent identity: annotation1919684
display id: annotation1919684
version: 1
top level: OmpA 5
location: https://synbiohub.org/public/igem/BBa_B0100/annotation1919684/range1919684/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_B0100/annotation1919684/range1919684/1
type: Range
persistent identity: range1919684
display id: range1919684
version: 1
top level: OmpA 5
direction: direction/forward
start: 104
end: 115
orientation: inline

HP3 3
type: ComponentDefinition
persistent identity: BBa B0101
display id: BBa_B0101
version: 1
was generated by: iGEM to SBOL conversion
description: HP3 3
created: 2007-02-28T12:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: The part was made by primer annealing.
owned by: james
owned by: myers
mutable description: 3' stabilizing mRNA designed by Christina Smolke and Jay Keasling for stabilizing gfp.
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 571
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: A single base pair change (A to T at position 25) from the original design was made in order to remove a PstI site occurring in the loop of the hairpin. This should have no effect on the secondary structure.
star: false
creator: Heather Keller
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0101_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation1919685
display id: annotation1919685
version: 1
top level: HP3 3
location: https://synbiohub.org/public/igem/BBa_B0101/annotation1919685/range1919685/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0101/annotation1919685/range1919685/1
type: Range
persistent identity: range1919685
display id: range1919685
version: 1
top level: HP3 3
direction: direction/forward
start: 6
end: 58
orientation: inline

AvrII/XbaI
type: ComponentDefinition
persistent identity: BBa B0104
display id: BBa_B0104
version: 1
was generated by: iGEM to SBOL conversion
description: AvrII/XbaI mixed site
created: 2007-03-06T12:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: n/a
owned by: james
owned by: myers
mutable description: This is the 6 bp mixed site that results when ligating the compatible ends (CTAG 5' overhang) that result from AvrII cleavage (CCTAGA) of the upstream part and XbaI (TCTAGA) cleavage of the downstream part.
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 571
owning group id: 10
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: n/a
star: false
creator: Heather Keller
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: AvrII/XbaI mixed site
sequence: https://synbiohub.org/public/igem/BBa_B0104_sequence/1

AvrII/XbaI mixed site
type: SequenceAnnotation
persistent identity: annotation1920669
display id: annotation1920669
version: 1
top level: AvrII/XbaI
location: https://synbiohub.org/public/igem/BBa_B0104/annotation1920669/range1920669/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_B0104/annotation1920669/range1920669/1
type: Range
persistent identity: range1920669
display id: range1920669
version: 1
top level: AvrII/XbaI
direction: direction/forward
start: 1
end: 6
orientation: inline

BBa_B2022
type: ComponentDefinition
persistent identity: BBa B2022
display id: BBa_B2022
version: 1
was generated by: iGEM to SBOL conversion
description: 4B RBS from T7
created: 2007-05-22T11:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: to be added automatically later
owned by: james
owned by: myers
mutable description: to be added automatically later
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 135
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: to be added automatically later
star: false
creator: Bartholomew Canton
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_B2022_sequence/1

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

mCherry
type: ComponentDefinition
persistent identity: BBa J06504
display id: BBa_J06504
version: 1
was generated by: iGEM to SBOL conversion
description: monomeric RFP optimized for bacteria
created: 2005-07-17T11:00:00Z
modified: 2016-01-25T01:05:28Z
mutable provenance: mCherry from Roger Y. Tsien's lab, altered to be BioBrick compatible
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: mRFP (DsRed) derived, altered to be a biobrick by removing a PstI site and adding in the ends.
discontinued: false
dominant: false
experience: experience/None
group u list: _20_
m user id: 4206
owner id: 340
owning group id: 20
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Made a point mutation to eliminate a PstI site in the middle and then added BioBrick ends using PCR. Sequenced using primers that bind to the pSB1A2 plasmid on either side of the brick.
star: true
creator: ytwang
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence annotation: mCherry
sequence annotation: C->T (removing PstI site)
sequence: https://synbiohub.org/public/igem/BBa_J06504_sequence/1

mCherry
type: SequenceAnnotation
persistent identity: annotation1585829
display id: annotation1585829
version: 1
top level: mCherry
location: https://synbiohub.org/public/igem/BBa_J06504/annotation1585829/range1585829/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_J06504/annotation1585829/range1585829/1
type: Range
persistent identity: range1585829
display id: range1585829
version: 1
top level: mCherry
direction: direction/forward
start: 1
end: 711
orientation: inline

C->T (removing PstI site)
type: SequenceAnnotation
persistent identity: annotation1585833
display id: annotation1585833
version: 1
top level: mCherry
location: https://synbiohub.org/public/igem/BBa_J06504/annotation1585833/range1585833/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_J06504/annotation1585833/range1585833/1
type: Range
persistent identity: range1585833
display id: range1585833
version: 1
top level: mCherry
direction: direction/forward
start: 352
end: 352
orientation: inline

lacI+pL
type: ComponentDefinition
persistent identity: BBa R0011
display id: BBa_R0011
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (lacI regulated, lambda pL hybrid)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site. LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
star: true
creator: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0011
sequence annotation: lac O1
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0011_sequence/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation7064
display id: annotation7064
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
type: Range
persistent identity: range7064
display id: range7064
version: 1
top level: lacI+pL
direction: direction/forward
start: 1
end: 54
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation1999
display id: annotation1999
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
type: Range
persistent identity: range1999
display id: range1999
version: 1
top level: lacI+pL
direction: direction/forward
start: 3
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2000
display id: annotation2000
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
type: Range
persistent identity: range2000
display id: range2000
version: 1
top level: lacI+pL
direction: direction/forward
start: 20
end: 25
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2001
display id: annotation2001
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
type: Range
persistent identity: range2001
display id: range2001
version: 1
top level: lacI+pL
direction: direction/forward
start: 26
end: 42
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002
display id: annotation2002
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
type: Range
persistent identity: range2002
display id: range2002
version: 1
top level: lacI+pL
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_R0183
type: ComponentDefinition
persistent identity: BBa R0183
display id: BBa_R0183
version: 1
was generated by: iGEM to SBOL conversion
description: T7 RNAP promoter
created: 2005-09-25T11:00:00Z
modified: 2015-05-08T01:14:15Z
owned by: james
owned by: myers
mutable description: T7 promoter with an A->C mutation at the -16 position of the consensus sequence
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Barry Canton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_R0183_sequence/1

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_B0073
type: ComponentDefinition
persistent identity: BBa B0073
display id: BBa_B0073
version: 1
was generated by: iGEM to SBOL conversion
description: Specialized RBS
created: 2006-05-01T11:00:00Z
modified: 2015-08-31T04:07:21Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Bartholomew Canton
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0073_sequence/1

BBa_B0016
type: ComponentDefinition
persistent identity: BBa B0016
display id: BBa_B0016
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (T7 RNAP specific, T_Phi)
created: 2003-12-04T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Sri Kosuri
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: T7 gene 1 specific transcriptional terminator
discontinued: false
dominant: true
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Sri Kosuri
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_B0016_sequence/1

https://synbiohub.org/public/igem/BBa_I2052_sequence/1
type: Sequence
persistent identity: BBa I2052 sequence
display id: BBa_I2052_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagaggccaggggtgctcggcataagccgaagatatcggtagagttaatattgagcagatcccccggtgaaggatttaaccgtgttatctcgttggagatattcatggcgtattttggattactagagcctagatactagagaaccctcaggaggtaaaccatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataatactagaggatcgcctgatcccggtgcacccgggcagctgcatagtctgggtgcaccgggatcaggtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcatacgactcactatagggagatactagagtcacaccactactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagctagcataaccccttggggcctctaaacgggtcttgaggggttttttg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0100_sequence/1
type: Sequence
persistent identity: BBa B0100 sequence
display id: BBa_B0100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gccaggggtgctcggcataagccgaagatatcggtagagttaatattgagcagatcccccggtgaaggatttaaccgtgttatctcgttggagatattcatggcgtattttggat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0101_sequence/1
type: Sequence
persistent identity: BBa B0101 sequence
display id: BBa_B0101_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gatcgcctgatcccggtgcacccgggcagctgcatagtctgggtgcaccgggatcagg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0104_sequence/1
type: Sequence
persistent identity: BBa B0104 sequence
display id: BBa_B0104_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cctaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B2022_sequence/1
type: Sequence
persistent identity: BBa B2022 sequence
display id: BBa_B2022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaccctcaggaggtaaacca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J06504_sequence/1
type: Sequence
persistent identity: BBa J06504 sequence
display id: BBa_J06504_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0011_sequence/1
type: Sequence
persistent identity: BBa R0011 sequence
display id: BBa_R0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0183_sequence/1
type: Sequence
persistent identity: BBa R0183 sequence
display id: BBa_R0183_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcatacgactcactatagggaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0073_sequence/1
type: Sequence
persistent identity: BBa B0073 sequence
display id: BBa_B0073_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacaccac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0016_sequence/1
type: Sequence
persistent identity: BBa B0016 sequence
display id: BBa_B0016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctagcataaccccttggggcctctaaacgggtcttgaggggttttttg
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z