BBa_I20532 1 BBa_I20532 Bxb1 BP Data Register 2012-04-02T11:00:00Z 2015-08-31T04:07:41Z Multiple The DNA data register in BP and LR states consist of a constitutive promoter (BBa_J23119) flanked by BP or LR recombination sites positioned in opposite orientation, resulting in DNA inversion when recombined. A Rrnp T1 terminator (BBa_J61048) was added in reverse orientation upstream of the promoter to prevent transcriptional read-through in the opposite orientation, so that in each state, only one fluorescent protein is visibly expressed. On each side of the recombination target, we cloned superfolder GFP and mKate2 under translational control of measured strong RBSs (BIOFAB pilot C-dog project http://biofab.org/data). false false _11_ 0 3868 11 Not in stock false Recombination sites can have some terminator activity-Strong RBS preferabe at low copy number false Jerome Bonnet annotation2171814 1 mKate2 range2171814 1 1 696 annotation2171815 1 Invitrogen RBS range2171815 1 697 726 annotation2171820 1 Superfolder GFP range2171820 1 1301 2020 annotation2171819 1 JBEI range2171819 1 1267 1300 annotation2171822 1 attP Bxb1 range2171822 1 1211 1263 annotation2171817 1 J61048 terminator range2171817 1 830 942 annotation2171816 1 AttB Bxb1 range2171816 1 727 776 annotation2171821 1 B0015 range2171821 1 2021 2149 annotation2171818 1 J23119 range2171818 1 1035 1069 BBa_I20532_sequence 1 ttatcaacgatgtcctaatttcgacggaaggtcacaataacgggctacggctacttcatgttgttcaacgtaggtttctttatcagcttctttaatacgttctaagcgacgatcaacatagtatactccaggcattttaaggtttttagcgggttttttggaacgataggttgttttcaggttgcagattaaatgaccgcctccaactaatttcagtgccatgtctgcgcgtccttctaagccaccgtccgcaggatataaggtttctgttgatgcttcccaaccaagagtttttttttgcattacgggtccgttactcgggaaatttacgccacggattttaacattgtaaatcagacagccatcttgtaaactcgtatcttgagttgctgtaagtacaccgccatcttcataagttgttacacgttcccaggtaaagccttcaggaaaggactgtttaaaaaagtcaggaatgccttgggtgtgattgataaaagttttagacccgtacataaagctcgttgcgagaatgtcaaacgcaaagggcaggggtccaccttcaactgctttgatgcgcattgtttgtgtgccttcatatggtttaccttcaccttcggatgtgcatttgaaatgatgattgttgacagtaccttccatatataatttcatgtgcatattttctttaattaattctgacattaatatatacctcttaatttttactagtcggccggcttgtcgacgacggcggtctccgtcgtcaggatcatccgggcccctcctgaggtgccacccgtgcggcacgttgaagccgattagtacaatagattttcttttgggtatagcgtcgtggacagtcattcatctttctgcccctccaaaagcaaaaacccgccgaagcgggtttttacgtaaatcaggtgaaactgaccgataagccggttattcaaccccaaaggtctactaccgtgctaacgctcgctaaggcccggtccggaagcacgccgtcgctctcgcgcctatacagttgtcacttgacagctagctcagtcctaggtataatgctagcagatggttagtgtgcgtatctgatacttttatgtctgtcctacaactccaagcgtacagttcaggccaacaccctagctttgagaaagtcgcgtcagtaaatgtcgtttccctaaagcttacaatttacctcctcgctattgggtttgtaccgtacaccactgagaccgcggtggttgaccagacaaaccacgatactagagaaaagatcttttaagaaggagatatacatatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaatgatgaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z