BBa_I20532
1
BBa_I20532
Bxb1 BP Data Register
2012-04-02T11:00:00Z
2015-08-31T04:07:41Z
Multiple
The DNA data register in BP and LR states consist of a constitutive promoter (BBa_J23119) flanked by BP or LR recombination sites positioned in opposite orientation, resulting in DNA inversion when recombined. A Rrnp T1 terminator (BBa_J61048) was added in reverse orientation upstream of the promoter to prevent transcriptional read-through in the opposite orientation, so that in each state, only one fluorescent protein is visibly expressed. On each side of the recombination target, we cloned superfolder GFP and mKate2 under translational control of measured strong RBSs (BIOFAB pilot C-dog project http://biofab.org/data).
false
false
_11_
0
3868
11
Not in stock
false
Recombination sites can have some terminator activity-Strong RBS preferabe at low copy number
false
Jerome Bonnet
annotation2171814
1
mKate2
range2171814
1
1
696
annotation2171815
1
Invitrogen RBS
range2171815
1
697
726
annotation2171820
1
Superfolder GFP
range2171820
1
1301
2020
annotation2171819
1
JBEI
range2171819
1
1267
1300
annotation2171822
1
attP Bxb1
range2171822
1
1211
1263
annotation2171817
1
J61048 terminator
range2171817
1
830
942
annotation2171816
1
AttB Bxb1
range2171816
1
727
776
annotation2171821
1
B0015
range2171821
1
2021
2149
annotation2171818
1
J23119
range2171818
1
1035
1069
BBa_I20532_sequence
1
ttatcaacgatgtcctaatttcgacggaaggtcacaataacgggctacggctacttcatgttgttcaacgtaggtttctttatcagcttctttaatacgttctaagcgacgatcaacatagtatactccaggcattttaaggtttttagcgggttttttggaacgataggttgttttcaggttgcagattaaatgaccgcctccaactaatttcagtgccatgtctgcgcgtccttctaagccaccgtccgcaggatataaggtttctgttgatgcttcccaaccaagagtttttttttgcattacgggtccgttactcgggaaatttacgccacggattttaacattgtaaatcagacagccatcttgtaaactcgtatcttgagttgctgtaagtacaccgccatcttcataagttgttacacgttcccaggtaaagccttcaggaaaggactgtttaaaaaagtcaggaatgccttgggtgtgattgataaaagttttagacccgtacataaagctcgttgcgagaatgtcaaacgcaaagggcaggggtccaccttcaactgctttgatgcgcattgtttgtgtgccttcatatggtttaccttcaccttcggatgtgcatttgaaatgatgattgttgacagtaccttccatatataatttcatgtgcatattttctttaattaattctgacattaatatatacctcttaatttttactagtcggccggcttgtcgacgacggcggtctccgtcgtcaggatcatccgggcccctcctgaggtgccacccgtgcggcacgttgaagccgattagtacaatagattttcttttgggtatagcgtcgtggacagtcattcatctttctgcccctccaaaagcaaaaacccgccgaagcgggtttttacgtaaatcaggtgaaactgaccgataagccggttattcaaccccaaaggtctactaccgtgctaacgctcgctaaggcccggtccggaagcacgccgtcgctctcgcgcctatacagttgtcacttgacagctagctcagtcctaggtataatgctagcagatggttagtgtgcgtatctgatacttttatgtctgtcctacaactccaagcgtacagttcaggccaacaccctagctttgagaaagtcgcgtcagtaaatgtcgtttccctaaagcttacaatttacctcctcgctattgggtttgtaccgtacaccactgagaccgcggtggttgaccagacaaaccacgatactagagaaaagatcttttaagaaggagatatacatatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaatgatgaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z