##gff-version 3
##sequence-region BBa_I20533 1 2011
BBa_I20533	.	terminator	1	129	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_I20533	.	terminator	1	80	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I20533	.	stem_loop	12	55	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I20533	.	engineered_region	1	80	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I20533	.	terminator	89	129	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I20533	.	stop_codon	122	122	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I20533	.	engineered_region	89	129	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I20533	.	stem_loop	96	115	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I20533	.	polyA_site	116	129	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I20533	.	CDS	130	904	.	+	0	ID=BBa_C0051;Name=BBa_C0051
BBa_I20533	.	tag	841	873	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_I20533	.	engineered_tag	880	904	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_I20533	.	CDS	133	840	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_I20533	.	terminator	905	950	.	+	0	ID=BBa_B0011;Name=BBa_B0011
BBa_I20533	.	stem_loop	917	939	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_I20533	.	engineered_region	905	950	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_I20533	.	engineered_region	951	2011	.	+	0	ID=BBa_F2620;Name=BBa_F2620
BBa_I20533	.	ribosome_entry_site	1013	1024	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F2620
BBa_I20533	.	conserved_region	1017	1020	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I20533	.	promoter	951	1004	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_F2620
BBa_I20533	.	promoter	970	975	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I20533	.	non_covalent_binding_site	976	994	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I20533	.	engineered_region	951	1004	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I20533	.	non_covalent_binding_site	951	969	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I20533	.	promoter	993	998	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I20533	.	terminator	1820	1948	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_F2620
BBa_I20533	.	terminator	1820	1899	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I20533	.	stem_loop	1831	1874	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I20533	.	engineered_region	1820	1899	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I20533	.	terminator	1908	1948	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I20533	.	stop_codon	1941	1941	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I20533	.	engineered_region	1908	1948	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I20533	.	stem_loop	1915	1934	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I20533	.	polyA_site	1935	1948	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I20533	.	promoter	1957	2011	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_F2620
BBa_I20533	.	non_covalent_binding_site	1957	1976	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_I20533	.	promoter	1998	2003	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_I20533	.	promoter	1976	1981	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_I20533	.	start_codon	2009	2009	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_I20533	.	engineered_region	1957	2011	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_I20533	.	CDS	1031	1786	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_F2620
BBa_I20533	.	engineered_region	1031	1786	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_I20533	.	CDS	1031	1780	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_I20533	.	engineered_region	1031	1032	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_I20533	.	point_mutation	1204	1204	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_I20533	.	engineered_tag	1787	1811	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_I20533	.	point_mutation	1522	1522	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
>BBa_I20533
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttataatgagcacaaaaaagaaaccattaacaca
agagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatct
gtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacg
ccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgta
tgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttc
tcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcat
tctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattct
cgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaa
ctgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgtt
ccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagt
agcttaataacgctgatagtgctagtgtagatcgcagagaatataaaaagccagattattaatccggcttttttattat
tttccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatac
tagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatatta
atcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggt
taaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatat
gatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaa
aatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaa
tggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaac
ataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaa
ccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcag
tgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaa
gcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactag
agccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgct
ctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacagg
tttacgcaagaaaatggtttgttatagtcgaataaa