LOCUS       BBa_I2056                903 bp    DNA     linear       27-Dec-2024
DEFINITION  RFP-based RBS tester
ACCESSION   BBa_I2056
VERSION     BBa_I2056.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     CDS             61..735
                     /label=mrfp1
     misc_feature    742..766
                     /label=Help:Barcodes
     CDS             61..741
                     /label=BBa_E1010
     stem_loop       786..829
                     /label=stem_loop
     misc_feature    775..854
                     /label=BBa_B0010
     terminator      775..854
                     /label=BBa_B0010
     stem_loop       870..889
                     /label=T7 TE
     misc_feature    896..896
                     /label=stop
     polyA_site      890..903
                     /label=polya
     misc_feature    863..903
                     /label=BBa_B0012
     terminator      863..903
                     /label=BBa_B0012
     misc_feature    61..903
                     /label=BBa_J04650
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 atggcttcct ccgaagacgt tatcaaagag ttcatgcgtt tcaaagttcg tatggaaggt
      121 tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg aaggtcgtcc gtacgaaggt
      181 acccagaccg ctaaactgaa agttaccaaa ggtggtccgc tgccgttcgc ttgggacatc
      241 ctgtccccgc agttccagta cggttccaaa gcttacgtta aacacccggc tgacatcccg
      301 gactacctga aactgtcctt cccggaaggt ttcaaatggg aacgtgttat gaacttcgaa
      361 gacggtggtg ttgttaccgt tacccaggac tcctccctgc aagacggtga gttcatctac
      421 aaagttaaac tgcgtggtac caacttcccg tccgacggtc cggttatgca gaaaaaaacc
      481 atgggttggg aagcttccac cgaacgtatg tacccggaag acggtgctct gaaaggtgaa
      541 atcaaaatgc gtctgaaact gaaagacggt ggtcactacg acgctgaagt taaaaccacc
      601 tacatggcta aaaaaccggt tcagctgccg ggtgcttaca aaaccgacat caaactggac
      661 atcacctccc acaacgaaga ctacaccatc gttgaacagt acgaacgtgc tgaaggtcgt
      721 cactccaccg gtgcttaata acgctgatag tgctagtgta gatcgctact agagccaggc
      781 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
      841 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
      901 ata
//