LOCUS       BBa_I22000              1798 bp    DNA     linear       13-Jun-2024
DEFINITION  RBS Scaffold with GFP reporter
ACCESSION   BBa_I22000
VERSION     BBa_I22000.1  
FEATURES             Location/Qualifiers
     misc_feature    1..54
                     /label=BBa_R0011
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     stem_loop       56..118
                     /label=hp1
     misc_feature    119..129
                     /label=ss1
     stem_loop       130..158
                     /label=hp2
     misc_feature    159..170
                     /label=ss2
     misc_feature    56..170
                     /label=BBa_B0100
     misc_feature    171..176
                     /label=AvrII site
     misc_feature    171..176
                     /label=BBa_B0048
     misc_feature    1..176
                     /label=BBa_S00159
     CDS             213..518
                     /label=ccdB (rev)
     CDS             520..740
                     /label=ccdA- (rev)
     misc_feature    651..651
                     /label=T to C mutation
     misc_feature    779..784
                     /label=-10 (rev)
     misc_feature    802..807
                     /label=-35 (rev)
     misc_feature    185..859
                     /label=BBa_P1010
     misc_feature    868..874
                     /label=SapI recognition
     misc_feature    868..875
                     /label=BBa_B0102
     misc_feature    876..1595
                     /label=BBa_E0040
     misc_feature    868..1595
                     /label=BBa_E0041
     stem_loop       1609..1661
                     /label=stem_loop
     misc_feature    1604..1661
                     /label=BBa_B0101
     stem_loop       1681..1724
                     /label=stem_loop
     misc_feature    1670..1749
                     /label=BBa_B0010
     terminator      1670..1749
                     /label=BBa_B0010
     stem_loop       1765..1784
                     /label=T7 TE
     misc_feature    1791..1791
                     /label=stop
     polyA_site      1785..1798
                     /label=polya
     misc_feature    1758..1798
                     /label=BBa_B0012
     terminator      1758..1798
                     /label=BBa_B0012
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacagccag
       61 gggtgctcgg cataagccga agatatcggt agagttaata ttgagcagat cccccggtga
      121 aggatttaac cgtgttatct cgttggagat attcatggcg tattttggat cctaggtact
      181 agagactggc tgtgtataag ggagcctgac atttatattc cccagaacat caggttaatg
      241 gcgtttttga tgtcattttc gcggtggctg agatcagcca cttcttcccc gataacggag
      301 accggcacac tggccatatc ggtggtcatc atgcgccagc tttcatcccc gatatgcacc
      361 accgggtaaa gttcacggga gactttatct gacagcagac gtgcactggc cagggggatc
      421 accatccgtc gcccgggcgt gtcaataata tcactctgta catccacaaa cagacgataa
      481 cggctctctc ttttataggt gtaaacctta aactgcattt caccagcccc tgttctcgtc
      541 agcaaaagag ccgttcattt caataaaccg ggcgacctca gccatccctt cctgattttc
      601 cgctttccag cgttcggcac gcagacgacg ggcttcattc tgcatggttg tgcttaccag
      661 accggagata ttgacatcat atatgccttg agcaactgat agctgtcgct gtcaactgtc
      721 actgtaatac gctgcttcat agcatacctc tttttgacat acttcgggta tacatatcag
      781 tatatattct tataccgcaa aaatcagcgc gcaaatacgc atactgttat ctggctttta
      841 gtaagccgga tccacgcgtt actagaggct cttctatgcg taaaggagaa gaacttttca
      901 ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg
      961 tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca
     1021 ctactggaaa actacctgtt ccatggccaa cacttgtcac tactttcggt tatggtgttc
     1081 aatgctttgc gagataccca gatcatatga aacagcatga ctttttcaag agtgccatgc
     1141 ccgaaggtta tgtacaggaa agaactatat ttttcaaaga tgacgggaac tacaagacac
     1201 gtgctgaagt caagtttgaa ggtgataccc ttgttaatag aatcgagtta aaaggtattg
     1261 attttaaaga agatggaaac attcttggac acaaattgga atacaactat aactcacaca
     1321 atgtatacat catggcagac aaacaaaaga atggaatcaa agttaacttc aaaattagac
     1381 acaacattga agatggaagc gttcaactag cagaccatta tcaacaaaat actccaattg
     1441 gcgatggccc tgtcctttta ccagacaacc attacctgtc cacacaatct gccctttcga
     1501 aagatcccaa cgaaaagaga gaccacatgg tccttcttga gtttgtaaca gctgctggga
     1561 ttacacatgg catggatgaa ctatacaaat aataatacta gaggatcgcc tgatcccggt
     1621 gcacccgggc agctgcatag tctgggtgca ccgggatcag gtactagagc caggcatcaa
     1681 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     1741 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//