##gff-version 3
##sequence-region BBa_I23000 1 1792
BBa_I23000	.	terminator	1752	1792	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I23000	.	polyA_site	1779	1792	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I23000	.	stop_codon	1785	1785	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I23000	.	stem_loop	1759	1778	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I23000	.	engineered_region	1752	1792	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I23000	.	engineered_region	868	1589	.	+	0	ID=BBa_E0042;Name=BBa_E0042
BBa_I23000	.	sequence_alteration	1227	1227	.	+	0	ID=annotation1920226;Name=C->T (removing PstI site);Parent=BBa_E0042
BBa_I23000	.	engineered_region	868	875	.	+	0	ID=annotation1920224;Name=BBa_B0102;Parent=BBa_E0042
BBa_I23000	.	CDS	876	1586	.	+	0	ID=annotation1920225;Name=mCherry;Parent=BBa_E0042
BBa_I23000	.	sequence_feature	868	874	.	+	0	ID=annotation1920223;Name=SapI recognition;Parent=BBa_E0042
BBa_I23000	.	engineered_region	876	1589	.	+	0	ID=annotation1920227;Name=BBa_J06504;Parent=BBa_E0042
BBa_I23000	.	terminator	1664	1743	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I23000	.	stem_loop	1675	1718	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I23000	.	engineered_region	1664	1743	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I23000	.	engineered_region	185	859	.	+	0	ID=BBa_P1010;Name=BBa_P1010
BBa_I23000	.	CDS	213	518	.	+	0	ID=annotation1747332;Name=ccdB (rev);Parent=BBa_P1010
BBa_I23000	.	point_mutation	651	651	.	+	0	ID=annotation1747334;Name=T to C mutation;Parent=BBa_P1010
BBa_I23000	.	sequence_feature	779	784	.	+	0	ID=annotation1747335;Name=-10 (rev);Parent=BBa_P1010
BBa_I23000	.	sequence_feature	802	807	.	+	0	ID=annotation1747336;Name=-35 (rev);Parent=BBa_P1010
BBa_I23000	.	CDS	520	740	.	+	0	ID=annotation1747333;Name=ccdA- (rev);Parent=BBa_P1010
BBa_I23000	.	mature_transcript_region	1598	1655	.	+	0	ID=BBa_B0101;Name=BBa_B0101
BBa_I23000	.	stem_loop	1603	1655	.	+	0	ID=annotation1919685;Name=stem_loop;Parent=BBa_B0101
BBa_I23000	.	engineered_region	1	176	.	+	0	ID=BBa_S00159;Name=BBa_S00159
BBa_I23000	.	sequence_feature	171	176	.	+	0	ID=annotation1920205;Name=AvrII site;Parent=BBa_S00159
BBa_I23000	.	promoter	20	25	.	+	0	ID=annotation1920197;Name=-35;Parent=BBa_S00159
BBa_I23000	.	promoter	43	48	.	+	0	ID=annotation1920199;Name=-10;Parent=BBa_S00159
BBa_I23000	.	sequence_feature	159	170	.	+	0	ID=annotation1920203;Name=ss2;Parent=BBa_S00159
BBa_I23000	.	sequence_feature	119	129	.	+	0	ID=annotation1920201;Name=ss1;Parent=BBa_S00159
BBa_I23000	.	operator	26	42	.	+	0	ID=annotation1920198;Name=lac O1;Parent=BBa_S00159
BBa_I23000	.	engineered_region	171	176	.	+	0	ID=annotation1920206;Name=BBa_B0048;Parent=BBa_S00159
BBa_I23000	.	engineered_region	56	170	.	+	0	ID=annotation1920204;Name=BBa_B0100;Parent=BBa_S00159
BBa_I23000	.	engineered_region	1	54	.	+	0	ID=annotation1920195;Name=BBa_R0011;Parent=BBa_S00159
BBa_I23000	.	stem_loop	56	118	.	+	0	ID=annotation1920200;Name=hp1;Parent=BBa_S00159
BBa_I23000	.	stem_loop	130	158	.	+	0	ID=annotation1920202;Name=hp2;Parent=BBa_S00159
BBa_I23000	.	operator	3	19	.	+	0	ID=annotation1920196;Name=lac O1;Parent=BBa_S00159
>BBa_I23000
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacagccaggggtgctcggcataagccg
aagatatcggtagagttaatattgagcagatcccccggtgaaggatttaaccgtgttatctcgttggagatattcatgg
cgtattttggatcctaggtactagagactggctgtgtataagggagcctgacatttatattccccagaacatcaggtta
atggcgtttttgatgtcattttcgcggtggctgagatcagccacttcttccccgataacggagaccggcacactggcca
tatcggtggtcatcatgcgccagctttcatccccgatatgcaccaccgggtaaagttcacgggagactttatctgacag
cagacgtgcactggccagggggatcaccatccgtcgcccgggcgtgtcaataatatcactctgtacatccacaaacaga
cgataacggctctctcttttataggtgtaaaccttaaactgcatttcaccagcccctgttctcgtcagcaaaagagccg
ttcatttcaataaaccgggcgacctcagccatcccttcctgattttccgctttccagcgttcggcacgcagacgacggg
cttcattctgcatggttgtgcttaccagaccggagatattgacatcatatatgccttgagcaactgatagctgtcgctg
tcaactgtcactgtaatacgctgcttcatagcatacctctttttgacatacttcgggtatacatatcagtatatattct
tataccgcaaaaatcagcgcgcaaatacgcatactgttatctggcttttagtaagccggatccacgcgttactagaggc
tcttctatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagg
gctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagct
gaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctac
gtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaact
tcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcg
cggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtac
cccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtca
agaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctccca
caacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtac
aagtaataatactagaggatcgcctgatcccggtgcacccgggcagctgcatagtctgggtgcaccgggatcaggtact
agagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacg
ctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata