LOCUS BBa_I3001 14040 bp DNA linear 26-Dec-2024 DEFINITION E. colibrator design 2, Polkadorks (I3410.I3200.E0422.I3510.I3401.I 3411.B0015.I3100.I3701.I3300.I370 ACCESSION BBa_I3001 VERSION BBa_I3001.1 FEATURES Location/Qualifiers promoter 38..43 /label=-10 promoter 15..20 /label=-35 misc_feature 25..41 /label=OR1 misc_feature 1..17 /label=OR2 misc_feature 1..49 /label=BBa_R0051 promoter 1..49 /label=BBa_R0051 misc_feature 62..65 /label=conserved RBS 58..69 /label=BBa_B0034 misc_feature 814..819 /label=2 CDS 76..819 /label=cI HK022 misc_feature 781..813 /label=SsrA misc_feature 76..83 /label=OR3 partial misc_feature 76..819 /label=BBa_C0050 misc_feature 820..844 /label=Help:Barcodes CDS 76..819 /label=BBa_C0050 misc_feature 857..860 /label=conserved RBS 853..864 /label=BBa_B0034 CDS 871..2349 /label=AspA misc_feature 871..871 /label=G misc_feature 1458..1458 /label=C misc_feature 1494..1494 /label=G misc_feature 1527..1527 /label=C misc_feature 1869..1869 /label=G misc_feature 1995..1995 /label=G misc_feature 2350..2382 /label=LVA misc_feature 2389..2413 /label=Help:Barcodes CDS 871..2388 /label=BBa_C0083 misc_feature 2426..2429 /label=conserved RBS 2422..2433 /label=BBa_B0034 CDS 2440..3201 /label=CFP (LVA) misc_feature 3158..3195 /label=SsrA misc_feature 3196..3201 /label=2 misc_feature 2508..2508 /label=A misc_feature 2440..3201 /label=BBa_E0022 misc_feature 2440..3201 /label=BBa_E0022 stem_loop 3221..3264 /label=stem_loop misc_feature 3210..3289 /label=BBa_B0010 terminator 3210..3289 /label=BBa_B0010 stem_loop 3305..3324 /label=T7 TE misc_feature 3331..3331 /label=stop polyA_site 3325..3338 /label=polya misc_feature 3298..3338 /label=BBa_B0012 terminator 3298..3338 /label=BBa_B0012 misc_feature 3347..3366 /label=LuxR/HSL promoter 3461..3468 /label=-10 promoter 3435..3440 /label=-35 misc_feature 3474..3474 /label=start misc_feature 3476..3495 /label=Putative LuxR/HSL misc_feature 3347..3497 /label=BBa_R0063 promoter 3347..3497 /label=BBa_R0063 misc_feature 3510..3513 /label=conserved RBS 3506..3517 /label=BBa_B0034 misc_feature 3524..3525 /label=prefix misc_feature 3697..3697 /label=T misc_feature 4015..4015 /label=A CDS 3524..4273 /label=luxR misc_feature 3524..4279 /label=BBa_C0062 misc_feature 4280..4304 /label=Help:Barcodes CDS 3524..4279 /label=BBa_C0062 stem_loop 4324..4367 /label=stem_loop misc_feature 4313..4392 /label=BBa_B0010 terminator 4313..4392 /label=BBa_B0010 stem_loop 4408..4427 /label=T7 TE misc_feature 4434..4434 /label=stop polyA_site 4428..4441 /label=polya misc_feature 4401..4441 /label=BBa_B0012 terminator 4401..4441 /label=BBa_B0012 misc_feature 4450..4469 /label=LuxR/HSL promoter 4469..4474 /label=-35 promoter 4491..4496 /label=-10 misc_feature 4502..4502 /label=start misc_feature 4450..4504 /label=BBa_R0062 promoter 4450..4504 /label=BBa_R0062 misc_feature 4517..4520 /label=conserved RBS 4513..4524 /label=BBa_B0034 CDS 4534..5241 /label=cI lambda misc_feature 5242..5274 /label=LVA misc_feature 5281..5305 /label=Help:Barcodes CDS 4531..5280 /label=BBa_C0051 stem_loop 5325..5368 /label=stem_loop misc_feature 5314..5393 /label=BBa_B0010 terminator 5314..5393 /label=BBa_B0010 stem_loop 5409..5428 /label=T7 TE misc_feature 5435..5435 /label=stop polyA_site 5429..5442 /label=polya misc_feature 5402..5442 /label=BBa_B0012 terminator 5402..5442 /label=BBa_B0012 promoter 5458..5463 /label=-35 RBS 5463..5477 /label=OR2 promoter 5480..5485 /label=-10 RBS 5487..5501 /label=OR1 misc_feature 5493..5493 /label=putative misc_feature 5451..5505 /label=BBa_R0050 promoter 5451..5505 /label=BBa_R0050 misc_feature 5518..5521 /label=conserved RBS 5514..5525 /label=BBa_B0034 CDS 5535..6242 /label=cI lambda misc_feature 6243..6275 /label=LVA misc_feature 6282..6306 /label=Help:Barcodes CDS 5532..6281 /label=BBa_C0051 stem_loop 6326..6369 /label=stem_loop misc_feature 6315..6394 /label=BBa_B0010 terminator 6315..6394 /label=BBa_B0010 stem_loop 6410..6429 /label=T7 TE misc_feature 6436..6436 /label=stop polyA_site 6430..6443 /label=polya misc_feature 6403..6443 /label=BBa_B0012 terminator 6403..6443 /label=BBa_B0012 promoter 6486..6491 /label=-10 promoter 6466..6471 /label=-35 promoter 6452..6500 /label=BBa_R1075 misc_feature 6513..6516 /label=conserved RBS 6509..6520 /label=BBa_B0034 misc_feature 7616..7654 /label=LVA CDS 6527..7654 /label=lacI-LVA misc_feature 6527..7654 /label=BBa_C0012 misc_feature 7655..7679 /label=Help:Barcodes CDS 6527..7654 /label=BBa_C0012 stem_loop 7699..7742 /label=stem_loop misc_feature 7688..7767 /label=BBa_B0010 terminator 7688..7767 /label=BBa_B0010 stem_loop 7783..7802 /label=T7 TE misc_feature 7809..7809 /label=stop polyA_site 7803..7816 /label=polya misc_feature 7776..7816 /label=BBa_B0012 terminator 7776..7816 /label=BBa_B0012 CDS 7825..7912 /label=end of LacI coding region (inactive) misc_feature 7825..8024 /label=BBa_R0010 misc_feature 7913..7950 /label=CAP binding site promoter 7961..7966 /label=-35 promoter 7985..7990 /label=-10 misc_feature 7990..8024 /label=LacI binding site misc_feature 7997..7997 /label=start promoter 7825..8024 /label=BBa_R0010 misc_feature 8037..8040 /label=conserved RBS 8033..8044 /label=BBa_B0034 CDS 8051..8926 /label=araC misc_feature 8927..8959 /label=LVA misc_feature 8966..8990 /label=Help:Barcodes CDS 8051..8965 /label=BBa_C0080 stem_loop 9010..9053 /label=stem_loop misc_feature 8999..9078 /label=BBa_B0010 terminator 8999..9078 /label=BBa_B0010 stem_loop 9094..9113 /label=T7 TE misc_feature 9120..9120 /label=stop polyA_site 9114..9127 /label=polya misc_feature 9087..9127 /label=BBa_B0012 terminator 9087..9127 /label=BBa_B0012 promoter 9170..9175 /label=-10 promoter 9150..9155 /label=-35 promoter 9136..9184 /label=BBa_R1075 misc_feature 9197..9200 /label=conserved RBS 9193..9204 /label=BBa_B0034 misc_feature 9633..9634 /label=A misc_feature 10663..10695 /label=LVA CDS 9211..9975 /label=Tar (front) CDS 9982..10662 /label=Envz (back) misc_feature 10702..10726 /label=Help:Barcodes CDS 9211..10701 /label=BBa_C0082 stem_loop 10746..10789 /label=stem_loop misc_feature 10735..10814 /label=BBa_B0010 terminator 10735..10814 /label=BBa_B0010 stem_loop 10830..10849 /label=T7 TE misc_feature 10856..10856 /label=stop polyA_site 10850..10863 /label=polya misc_feature 10823..10863 /label=BBa_B0012 terminator 10823..10863 /label=BBa_B0012 misc_feature 10884..10901 /label=C1 OmpR misc_feature 10905..10922 /label=C2 OmpR misc_feature 10925..10942 /label=C3 OmpR promoter 10946..10951 /label=-35 promoter 10969..10974 /label=-10 promoter 10872..10979 /label=BBa_R0082 misc_feature 10992..10995 /label=conserved RBS 10988..10999 /label=BBa_B0034 CDS 11006..11881 /label=araC misc_feature 11882..11914 /label=LVA misc_feature 11921..11945 /label=Help:Barcodes CDS 11006..11920 /label=BBa_C0080 stem_loop 11965..12008 /label=stem_loop misc_feature 11954..12033 /label=BBa_B0010 terminator 11954..12033 /label=BBa_B0010 stem_loop 12049..12068 /label=T7 TE misc_feature 12075..12075 /label=stop polyA_site 12069..12082 /label=polya misc_feature 12042..12082 /label=BBa_B0012 terminator 12042..12082 /label=BBa_B0012 misc_feature 12094..12119 /label=c-amp2 misc_feature 12096..12134 /label=araO1 misc_feature 12133..12162 /label=c-amp1 misc_feature 12163..12191 /label=ara1 and ara2 promoter 12203..12208 /label=-35 promoter 12226..12231 /label=-10 promoter 12091..12239 /label=BBa_R0080 misc_feature 12252..12255 /label=conserved RBS 12248..12259 /label=BBa_B0034 misc_feature 13004..13009 /label=2 CDS 12266..13009 /label=cI HK022 misc_feature 12971..13003 /label=SsrA misc_feature 12266..12273 /label=OR3 partial misc_feature 12266..13009 /label=BBa_C0050 misc_feature 13010..13034 /label=Help:Barcodes CDS 12266..13009 /label=BBa_C0050 stem_loop 13054..13097 /label=stem_loop misc_feature 13043..13122 /label=BBa_B0010 terminator 13043..13122 /label=BBa_B0010 stem_loop 13138..13157 /label=T7 TE misc_feature 13164..13164 /label=stop polyA_site 13158..13171 /label=polya misc_feature 13131..13171 /label=BBa_B0012 terminator 13131..13171 /label=BBa_B0012 misc_feature 13180..13199 /label=LuxR/HSL promoter 13199..13204 /label=-35 promoter 13221..13226 /label=-10 misc_feature 13232..13232 /label=start misc_feature 13180..13234 /label=BBa_R0062 promoter 13180..13234 /label=BBa_R0062 misc_feature 13247..13250 /label=conserved RBS 13243..13254 /label=BBa_B0034 misc_feature 13840..13871 /label=LVA CDS 13261..13839 /label=luxI misc_feature 13261..13878 /label=BBa_C0061 misc_feature 13879..13903 /label=Help:Barcodes CDS 13261..13878 /label=BBa_C0061 stem_loop 13923..13966 /label=stem_loop misc_feature 13912..13991 /label=BBa_B0010 terminator 13912..13991 /label=BBa_B0010 stem_loop 14007..14026 /label=T7 TE misc_feature 14033..14033 /label=stop polyA_site 14027..14040 /label=polya misc_feature 14000..14040 /label=BBa_B0012 terminator 14000..14040 /label=BBa_B0012 ORIGIN 1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagaaa 61 gaggagaaat actagatggt tcaacagaaa gagcgtgaaa ctttctcgca gaggcttgcg 121 ctggcctgtg ataaagcggg attacctttg catggtaggc aggctgattt agctgtcagg 181 cttaaggtca caccaaaagc cattagtaaa tggttcaacg gggagtcaat accaagaaaa 241 gacaagatgg aatctctggc ttcggtgctg ggaactactg ctgcatatct gcatggctat 301 gctgatgatg acggtatcac ggtaaatcat ctatcaagat caaatgatta ttatcgtgtt 361 gatgtattgg atgttcaggc gagcgccggg ccaggaacca tggtttccaa tgaatttata 421 gaaaagataa gagcaattga atatacgacc gagcaggcaa gaattttatt taatggaagg 481 ccacaggaaa gcgtaaaagt catcacggtt cgcggtgaca gcatggaggg aaccatcaat 541 ccgggagatg agatctttgt tgatgtatcc ataacctgtt ttgatggcga tggcatttat 601 gtgtttgtat acgggaaaac aatgcacgtt aagcgcctgc aaatgcaaaa gaacaggctt 661 gccgtcatct ctgacaatgc cgcttatgat cgatggtaca tagaagaagg tgaagaagag 721 caacttcaca ttctagccaa agtcctcatt aggcagtcaa tcgattacaa gcgattcgga 781 gctgcaaacg acgaaaacta cgctttagta gcttaataac cctgatagtg ctagtgtaga 841 tccctactag agaaagagga gaaatactag atgtgtttaa agcaaatcat tggcagcttg 901 aaaaagaagg ttcacatgtc aaacaacatt cgtatcgaag aagatctgtt gggtaccagg 961 gaagttccag ctgatgccta ctatggtgtt cacactctga gagcgattga aaacttctat 1021 atcagcaaca acaaaatcag tgatattcct gaatttgttc gcggtatggt aatggttaaa 1081 aaagccgcag ctatggcaaa caaagagctg caaaccattc ctaaaagtgt agcgaatgcc 1141 atcattgccg catgtgatga agtcctgaac aacggaaaat gcatggatca gttcccggta 1201 gacgtctacc agggcggcgc aggtacttcc gtaaacatga acaccaacga agtgctggcc 1261 aatatcggtc tggaactgat gggtcaccaa aaaggtgaat atcagtacct gaacccgaac 1321 gaccatgtta acaaatgtca gtccactaac gacgcctacc cgaccggttt ccgtatcgca 1381 gtttactctt ccctgattaa gctggtagat gcgattaacc aactgcgtga aggctttgaa 1441 cgtaaagctg tcgaatttca ggacatcctg aaaatgggtc gtacccagct gcaagacgca 1501 gtaccgatga ccctcggtca ggaatttcgc gctttcagca tcctgctgaa agaagaagtg 1561 aaaaacatcc aacgtaccgc tgaactgctg ctggaagtta accttggtgc aacagcaatc 1621 ggtactggtc tgaacacgcc gaaagagtac tctccgctgg cagtgaaaaa actggctgaa 1681 gttactggct tcccatgcgt accggctgaa gacctgatcg aagcgacctc tgactgcggc 1741 gcttatgtta tggttcacgg cgcgctgaaa cgcctggctg tgaagatgtc caaaatctgt 1801 aacgacctgc gcttgctctc ttcaggccca cgtgccggcc tgaacgagat caacctgccg 1861 gaactgcaag cgggctcttc catcatgcca gctaaagtaa acccggttgt tccggaagtg 1921 gttaaccagg tatgcttcaa agtcatcggt aacgacacca ctgttaccat ggcagcagaa 1981 gcaggtcagc tgcaattgaa cgttatggag ccggtcattg gccaggccat gttcgaatcc 2041 gttcacattc tgaccaacgc ttgctacaac ctgctggaaa aatgcattaa cggcatcact 2101 gctaacaaag aagtgtgcga aggttacgtt tacaactcta tcggtatcgt tacttacctg 2161 aacccgttca tcggtcacca caacggtgac atcgtgggta aaatctgtgc cgaaaccggt 2221 aagagtgtac gtgaagtcgt tctggaacgc ggtctgttga ctgaagcgga acttgacgat 2281 attttctccg tacagaatct gatgcacccg gcttacaaag caaaacgcta tactgatgaa 2341 agcgaacagg ctgcaaacga cgaaaactac gctttagtag cttaataact ctgatagtgc 2401 tagtgtagat ctctactaga gaaagaggag aaatactaga tggtgagcaa gggcgaggag 2461 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtgaa cggccacaag 2521 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc 2581 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctgg 2641 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc 2701 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac 2761 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag 2821 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacatc 2881 agccacaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag 2941 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc 3001 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc 3061 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc 3121 gccgggatca ctctcggcat ggacgagctg tacaagaggc ctgctgcaaa cgacgaaaac 3181 tacgctttag tagcttaata atactagagc caggcatcaa ataaaacgaa aggctcagtc 3241 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 3301 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtacgatcct 3361 acaggtgctt atgttaagta attgtattcc cagcgataca atagtgtgac aaaaatccaa 3421 tttattagaa tcaaatgtca atccattacc gttttaatga tatataacac gcaaaacttg 3481 cgacaaacaa taggtaatac tagagaaaga ggagaaatac tagatgaaaa acataaatgc 3541 cgacgacaca tacagaataa ttaataaaat taaagcttgt agaagcaata atgatattaa 3601 tcaatgctta tctgatatga ctaaaatggt acattgtgaa tattatttac tcgcgatcat 3661 ttatcctcat tctatggtta aatctgatat ttcaatccta gataattacc ctaaaaaatg 3721 gaggcaatat tatgatgacg ctaatttaat aaaatatgat cctatagtag attattctaa 3781 ctccaatcat tcaccaatta attggaatat atttgaaaac aatgctgtaa ataaaaaatc 3841 tccaaatgta attaaagaag cgaaaacatc aggtcttatc actgggttta gtttccctat 3901 tcatacggct aacaatggct tcggaatgct tagttttgca cattcagaaa aagacaacta 3961 tatagatagt ttatttttac atgcgtgtat gaacatacca ttaattgttc cttctctagt 4021 tgataattat cgaaaaataa atatagcaaa taataaatca aacaacgatt taaccaaaag 4081 agaaaaagaa tgtttagcgt gggcatgcga aggaaaaagc tcttgggata tttcaaaaat 4141 attaggttgc agtgagcgta ctgtcacttt ccatttaacc aatgcgcaaa tgaaactcaa 4201 tacaacaaac cgctgccaaa gtatttctaa agcaatttta acaggagcaa ttgattgccc 4261 atactttaaa aattaataac actgatagtg ctagtgtaga tcactactag agccaggcat 4321 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 4381 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 4441 atactagaga cctgtaggat cgtacaggtt tacgcaagaa aatggtttgt tatagtcgaa 4501 taaatactag agaaagagga gaaatactag atgagcacaa aaaagaaacc attaacacaa 4561 gagcagcttg aggacgcacg tcgccttaaa gcaatttatg aaaaaaagaa aaatgaactt 4621 ggcttatccc aggaatctgt cgcagacaag atggggatgg ggcagtcagg cgttggtgct 4681 ttatttaatg gcatcaatgc attaaatgct tataacgccg cattgcttgc aaaaattctc 4741 aaagttagcg ttgaagaatt tagcccttca atcgccagag aaatctacga gatgtatgaa 4801 gcggttagta tgcagccgtc acttagaagt gagtatgagt accctgtttt ttctcatgtt 4861 caggcaggga tgttctcacc tgagcttaga acctttacca aaggtgatgc ggagagatgg 4921 gtaagcacaa ccaaaaaagc cagtgattct gcattctggc ttgaggttga aggtaattcc 4981 atgaccgcac caacaggctc caagccaagc tttcctgacg gaatgttaat tctcgttgac 5041 cctgagcagg ctgttgagcc aggtgatttc tgcatagcca gacttggggg tgatgagttt 5101 accttcaaga aactgatcag ggatagcggt caggtgtttt tacaaccact aaacccacag 5161 tacccaatga tcccatgcaa tgagagttgt tccgttgtgg ggaaagttat cgctagtcag 5221 tggcctgaag agacgtttgg cgctgcaaac gacgaaaact acgctttagt agcttaataa 5281 cgctgatagt gctagtgtag atcgctacta gagccaggca tcaaataaaa cgaaaggctc 5341 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga 5401 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag ttaggtattg 5461 actgtactat cagttccgtc ataatatgaa ccataagttc accactacta gagaaagagg 5521 agaaatacta gatgagcaca aaaaagaaac cattaacaca agagcagctt gaggacgcac 5581 gtcgccttaa agcaatttat gaaaaaaaga aaaatgaact tggcttatcc caggaatctg 5641 tcgcagacaa gatggggatg gggcagtcag gcgttggtgc tttatttaat ggcatcaatg 5701 cattaaatgc ttataacgcc gcattgcttg caaaaattct caaagttagc gttgaagaat 5761 ttagcccttc aatcgccaga gaaatctacg agatgtatga agcggttagt atgcagccgt 5821 cacttagaag tgagtatgag taccctgttt tttctcatgt tcaggcaggg atgttctcac 5881 ctgagcttag aacctttacc aaaggtgatg cggagagatg ggtaagcaca accaaaaaag 5941 ccagtgattc tgcattctgg cttgaggttg aaggtaattc catgaccgca ccaacaggct 6001 ccaagccaag ctttcctgac ggaatgttaa ttctcgttga ccctgagcag gctgttgagc 6061 caggtgattt ctgcatagcc agacttgggg gtgatgagtt taccttcaag aaactgatca 6121 gggatagcgg tcaggtgttt ttacaaccac taaacccaca gtacccaatg atcccatgca 6181 atgagagttg ttccgttgtg gggaaagtta tcgctagtca gtggcctgaa gagacgtttg 6241 gcgctgcaaa cgacgaaaac tacgctttag tagcttaata acgctgatag tgctagtgta 6301 gatcgctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt 6361 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc 6421 gggtgggcct ttctgcgttt atatactaga gttaaatttc ctcttttcag gccggaataa 6481 ctccctataa tgcgccacca tactagagaa agaggagaaa tactagatgg tgaatgtgaa 6541 accagtaacg ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg 6601 cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat 6661 ggcggagctg aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt 6721 gctgattggc gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc 6781 gattaaatct cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag 6841 cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct 6901 gatcattaac tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa 6961 tgttccggcg ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc 7021 ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat 7081 cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca 7141 taaatatctc actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc 7201 catgtccggt tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat 7261 gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct 7321 gcgcgttggt gcggatatct cggtagtggg atacgacgat accgaagaca gctcatgtta 7381 tatcccgccg ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga 7441 ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc 7501 actggtgaaa agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt 7561 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcaggctgc 7621 aaacgacgaa aactacgctt tagtagctta ataactctga tagtgctagt gtagatctct 7681 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt 7741 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg 7801 cctttctgcg tttatatact agagcaatac gcaaaccgcc tctccccgcg cgttggccga 7861 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg 7921 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg 7981 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacatactag agaaagagga 8041 gaaatactag atggctgaag cgcaaaatga tcccctgctg ccgggatact cgtttaacgc 8101 ccatctggtg gcgggtttaa cgccgattga ggccaatggt tatctcgatt tttttatcga 8161 ccgaccgctg ggaatgaaag gttatattct caatctcacc attcgcggtc agggggtggt 8221 gaaaaatcag ggacgagaat ttgtctgccg accgggtgat attttgctgt tcccgccagg 8281 agagattcat cactacggtc gtcatccgga ggctcgcgaa tggtatcacc agtgggttta 8341 ctttcgtccg cgcgcctact ggcatgaatg gcttaactgg ccgtcaatat ttgccaatac 8401 gggtttcttt cgcccggatg aagcgcacca gccgcatttc agcgacctgt ttgggcaaat 8461 cattaacgcc gggcaagggg aagggcgcta ttcggagctg ctggcgataa atctgcttga 8521 gcaattgtta ctgcggcgca tggaagcgat taacgagtcg ctccatccac cgatggataa 8581 tcgggtacgc gaggcttgtc agtacatcag cgatcacctg gcagacagca attttgatat 8641 cgccagcgtc gcacagcatg tttgcctgtc gccgtcgcgt ctgtcacatc ttttccgcca 8701 gcagttaggg attagcgtct taagctggcg cgaggaccaa cgcatcagcc aggcgaagct 8761 gcttttgagc actacccgga tgcctatcgc caccgtcggt cgcaatgttg gttttgacga 8821 tcaactctat ttctcgcgag tatttaaaaa atgcaccggg gccagcccga gcgagttccg 8881 tgccggttgt gaagaaaaag tgaatgatgt agccgtcaag ttgtcagctg caaacgacga 8941 aaactacgct ttagtagctt aataacactg atagtgctag tgtagatcac tactagagcc 9001 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt 9061 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc 9121 gtttatatac tagagttaaa tttcctcttt tcaggccgga ataactccct ataatgcgcc 9181 accatactag agaaagagga gaaatactag atgattaacc gtatccgcgt agtcacgctg 9241 ttggtaatgg tgctgggggt attcgcactg ttacagctta tttccggcag tctgtttttt 9301 tcttcccttc accatagcca gaagagcttt gtggtttcca atcaattacg ggaacagcag 9361 ggcgagctga cgtcaacctg ggatttaatg ctgcaaacgc gcattaacct gagtcgttca 9421 gcggtacgga tgatgatgga ttcctccaat caacaaagta acgccaaagt tgaattgctc 9481 gatagcgcca ggaaaacatt ggcgcaggca gcgacgcatt ataaaaaatt caaaagcatg 9541 gcaccgttac ctgaaatggt cgctaccagt cgtaatattg atgaaaaata taaaaactat 9601 tacacagcgt taactgaact gattgattat ctcgattatg gcaatactgg agcttatttc 9661 gctcagccaa cccagggaat gcaaaatgca atgggcgaag cgtttgctca gtacgccctc 9721 agcagtgaaa aactgtatcg cgatatcgtc actgacaacg cagatgatta ccgatttgcc 9781 cagtggcaac tggcggttat cgcgctggtg gtggtattga ttctgctggt ggcgtggtac 9841 ggcattcgcc gtatgttgct tactccgctg gcaaaaatta ttgctcacat tcgcgaaatc 9901 gccggtggta acctggcgaa taccctgacc attgacgggc gcagtgaaat gggcgacctg 9961 gcgcagagcg tttcacatat ggcggctggt gttaagcaac tggcggatga ccgcacgctg 10021 ctgatggcgg gggtaagtca cgacttgcgc acgccgctga cgcgtattcg cctggcgact 10081 gagatgatga gcgagcagga tggctatctg gcagaatcga tcaataaaga tatcgaagag 10141 tgcaacgcca tcattgagca gtttatcgac tacctgcgca ccgggcagga gatgccgatg 10201 gaaatggcgg atcttaatgc agtactcggt gaggtgattg ctgccgaaag tggctatgag 10261 cgggaaattg aaaccgcgct ttaccccggc agcattgaag tgaaaatgca cccgctgtcg 10321 atcaaacgcg cggtggcgaa tatggtggtc aacgccgccc gttatggcaa tggctggatc 10381 aaagtcagca gcggaacgga gccgaatcgc gcctggttcc aggtggaaga tgacggtccg 10441 ggaattgcgc cggaacaacg taagcacctg ttccagccgt ttgtccgcgg cgacagtgcg 10501 cgcaccatta gcggcacggg attagggctg gcaattgtgc agcgtatcgt ggataaccat 10561 aacgggatgc tggagcttgg caccagcgag cggggcgggc tttccattcg cgcctggctg 10621 ccagtgccgg taacgcgggc gcagggcacg acaaaagaag gggctgcaaa cgacgaaaac 10681 tacgctttag tagcttaata acgctgatag tgctagtgta gatcgctact agagccaggc 10741 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt 10801 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt 10861 atatactaga gtcccttgca tttacatttt gaaacatcta tagcgataaa tgaaacatct 10921 taaaagtttt agtatcatat tcgtgttgga ttattctgca tttttgggga gaatggactt 10981 actagagaaa gaggagaaat actagatggc tgaagcgcaa aatgatcccc tgctgccggg 11041 atactcgttt aacgcccatc tggtggcggg tttaacgccg attgaggcca atggttatct 11101 cgattttttt atcgaccgac cgctgggaat gaaaggttat attctcaatc tcaccattcg 11161 cggtcagggg gtggtgaaaa atcagggacg agaatttgtc tgccgaccgg gtgatatttt 11221 gctgttcccg ccaggagaga ttcatcacta cggtcgtcat ccggaggctc gcgaatggta 11281 tcaccagtgg gtttactttc gtccgcgcgc ctactggcat gaatggctta actggccgtc 11341 aatatttgcc aatacgggtt tctttcgccc ggatgaagcg caccagccgc atttcagcga 11401 cctgtttggg caaatcatta acgccgggca aggggaaggg cgctattcgg agctgctggc 11461 gataaatctg cttgagcaat tgttactgcg gcgcatggaa gcgattaacg agtcgctcca 11521 tccaccgatg gataatcggg tacgcgaggc ttgtcagtac atcagcgatc acctggcaga 11581 cagcaatttt gatatcgcca gcgtcgcaca gcatgtttgc ctgtcgccgt cgcgtctgtc 11641 acatcttttc cgccagcagt tagggattag cgtcttaagc tggcgcgagg accaacgcat 11701 cagccaggcg aagctgcttt tgagcactac ccggatgcct atcgccaccg tcggtcgcaa 11761 tgttggtttt gacgatcaac tctatttctc gcgagtattt aaaaaatgca ccggggccag 11821 cccgagcgag ttccgtgccg gttgtgaaga aaaagtgaat gatgtagccg tcaagttgtc 11881 agctgcaaac gacgaaaact acgctttagt agcttaataa cactgatagt gctagtgtag 11941 atcactacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt 12001 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg 12061 ggtgggcctt tctgcgttta tatactagag gcgtaacaaa agtgtctata atcacggcag 12121 aaaagtccac attgattatt tgcacggcgt cacactttgc tatgccatag catttttatc 12181 cataagatta gcggatccta cctgacgctt tttatcgcaa ctctctactg tttctccatt 12241 actagagaaa gaggagaaat actagatggt tcaacagaaa gagcgtgaaa ctttctcgca 12301 gaggcttgcg ctggcctgtg ataaagcggg attacctttg catggtaggc aggctgattt 12361 agctgtcagg cttaaggtca caccaaaagc cattagtaaa tggttcaacg gggagtcaat 12421 accaagaaaa gacaagatgg aatctctggc ttcggtgctg ggaactactg ctgcatatct 12481 gcatggctat gctgatgatg acggtatcac ggtaaatcat ctatcaagat caaatgatta 12541 ttatcgtgtt gatgtattgg atgttcaggc gagcgccggg ccaggaacca tggtttccaa 12601 tgaatttata gaaaagataa gagcaattga atatacgacc gagcaggcaa gaattttatt 12661 taatggaagg ccacaggaaa gcgtaaaagt catcacggtt cgcggtgaca gcatggaggg 12721 aaccatcaat ccgggagatg agatctttgt tgatgtatcc ataacctgtt ttgatggcga 12781 tggcatttat gtgtttgtat acgggaaaac aatgcacgtt aagcgcctgc aaatgcaaaa 12841 gaacaggctt gccgtcatct ctgacaatgc cgcttatgat cgatggtaca tagaagaagg 12901 tgaagaagag caacttcaca ttctagccaa agtcctcatt aggcagtcaa tcgattacaa 12961 gcgattcgga gctgcaaacg acgaaaacta cgctttagta gcttaataac cctgatagtg 13021 ctagtgtaga tccctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac 13081 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc 13141 tcaccttcgg gtgggccttt ctgcgtttat atactagaga cctgtaggat cgtacaggtt 13201 tacgcaagaa aatggtttgt tatagtcgaa taaatactag agaaagagga gaaatactag 13261 atgactataa tgataaaaaa atcggatttt ttggcaattc catcggagga gtataaaggt 13321 attctaagtc ttcgttatca agtgtttaag caaagacttg agtgggactt agttgtagaa 13381 aataaccttg aatcagatga gtatgataac tcaaatgcag aatatattta tgcttgtgat 13441 gatactgaaa atgtaagtgg atgctggcgt ttattaccta caacaggtga ttatatgctg 13501 aaaagtgttt ttcctgaatt gcttggtcaa cagagtgctc ccaaagatcc taatatagtc 13561 gaattaagtc gttttgctgt aggtaaaaat agctcaaaga taaataactc tgctagtgaa 13621 attacaatga aactatttga agctatatat aaacacgctg ttagtcaagg tattacagaa 13681 tatgtaacag taacatcaac agcaatagag cgatttttaa agcgtattaa agttccttgt 13741 catcgtattg gagacaaaga aattcatgta ttaggtgata ctaaatcggt tgtattgtct 13801 atgcctatta atgaacagtt taaaaaagca gtcttaaatg ctgcaaacga cgaaaactac 13861 gctttagtag cttaataact ctgatagtgc tagtgtagat ctctactaga gccaggcatc 13921 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 13981 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata //