BBa_I3001
type: ComponentDefinition
persistent identity: BBa I3001
display id: BBa_I3001
version: 1
was generated by: iGEM to SBOL conversion
description: E. colibrator design 2, Polkadorks (I3410.I3200.E0422.I3510.I3401.I3411.B0015.I3100.I3701.I3300.I370
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:07:41Z
owned by: james
owned by: myers
mutable description: E. colibrator design 2 has an explicit OR gate before the switch via an araC OR gate device (I3701, I3700). Design 2 has no explicit activity during the switch OFF state, as illustrated by the terminator immediately following I3411 Lambda cI switch output device. This causes the OFF state to rely completely on cellular diffusion to reset the system.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The layout of E. colibrator (I3001) is designed to insulate the most sensitive devices, namely those in the switch that store state. The hk022cI switch output (ON) is insulated by appearing first. This output is then attached to the AspA attractant sender and the CFP reporter (I3410.I3200.E0422). The quorum receiver and its control of the lambda cI switch input (OFF) occur next (I3510.I3401) because leaky TIPS through this module will encourage the OFF state dicated by the downstream lambda cI switch output (I3411.B0015). Note that the OFF switch output simply ends at termination of the output TIPS (B0015) in this design. The rest of the switch inputs occur downstream because they do not store state and therefore do not need to be as insulated from leaky TIPS. Lac trigger controls the araC input device for the OR gate (I3100.I3701), and Tar-EnvZ receptor (I3300 upstream ompC version) controls the second OR input, embedded in the araC buffer device (I3300.I3700). The araC OR gate in turn outputs TIPS into the hk022cI switch input (I3400). Finally, the quorum sender device (I3500) is last because it is meant to be constitutively on at some basal level, so insulation is not required. Note: Depending on the efficacy of the OmpR promoter, this design can be implemented with any of I3300, I3301, I3302 (variations of Tar-EnvZ devices with different OmpR promoters). The default implementation uses I3300.
star: false
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
type: DnaRegion
role: partType/Project
role: SO:0000804
component: BBa_C0083
component: BBa_B0034
component: BBa_C0050
component: BBa_B0012
component: BBa_B0010
component: BBa_C0080
component: BBa_C0061
component: BBa_R0010
component: BBa_C0082
component: BBa_R0062
component: BBa_R1075
component: BBa_R0082
component: BBa_R0080
component: BBa_C0051
component: BBa_C0012
component: BBa_R0050
component: BBa_E0022
component: BBa_C0062
component: BBa_R0063

BBa_C0083
type: Component
persistent identity: component1010561
display id: component1010561
version: 1
top level: BBa_I3001
definition: aspA
access: public

BBa_B0034
type: Component
persistent identity: component1010534
display id: component1010534
version: 1
top level: BBa_I3001
definition: BBa_B0034
access: public

BBa_C0050
type: Component
persistent identity: component1010526
display id: component1010526
version: 1
top level: BBa_I3001
definition: cI HK022
access: public

BBa_B0034
type: Component
persistent identity: component1010511
display id: component1010511
version: 1
top level: BBa_I3001
definition: BBa_B0034
access: public

BBa_B0012
type: Component
persistent identity: component1011062
display id: component1011062
version: 1
top level: BBa_I3001
definition: BBa_B0012
access: public

BBa_B0012
type: Component
persistent identity: component1011012
display id: component1011012
version: 1
top level: BBa_I3001
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1011002
display id: component1011002
version: 1
top level: BBa_I3001
definition: BBa_B0010
access: public

BBa_C0050
type: Component
persistent identity: component1010994
display id: component1010994
version: 1
top level: BBa_I3001
definition: cI HK022
access: public

BBa_B0010
type: Component
persistent identity: component1010842
display id: component1010842
version: 1
top level: BBa_I3001
definition: BBa_B0010
access: public

BBa_B0034
type: Component
persistent identity: component1010979
display id: component1010979
version: 1
top level: BBa_I3001
definition: BBa_B0034
access: public

BBa_C0080
type: Component
persistent identity: component1010836
display id: component1010836
version: 1
top level: BBa_I3001
definition: araC
access: public

BBa_B0010
type: Component
persistent identity: component1011052
display id: component1011052
version: 1
top level: BBa_I3001
definition: BBa_B0010
access: public

BBa_B0034
type: Component
persistent identity: component1010826
display id: component1010826
version: 1
top level: BBa_I3001
definition: BBa_B0034
access: public

BBa_C0061
type: Component
persistent identity: component1011045
display id: component1011045
version: 1
top level: BBa_I3001
definition: luxI
access: public

BBa_R0010
type: Component
persistent identity: component1010816
display id: component1010816
version: 1
top level: BBa_I3001
definition: LacI
access: public

BBa_B0034
type: Component
persistent identity: component1011035
display id: component1011035
version: 1
top level: BBa_I3001
definition: BBa_B0034
access: public

BBa_C0082
type: Component
persistent identity: component1010884
display id: component1010884
version: 1
top level: BBa_I3001
definition: tar-envZ
access: public

BBa_R0062
type: Component
persistent identity: component1011027
display id: component1011027
version: 1
top level: BBa_I3001
definition: lux pR
access: public

BBa_B0034
type: Component
persistent identity: component1010868
display id: component1010868
version: 1
top level: BBa_I3001
definition: BBa_B0034
access: public

BBa_B0034
type: Component
persistent identity: component1010925
display id: component1010925
version: 1
top level: BBa_I3001
definition: BBa_B0034
access: public

BBa_R1075
type: Component
persistent identity: component1010863
display id: component1010863
version: 1
top level: BBa_I3001
definition: const.
access: public

BBa_R0082
type: Component
persistent identity: component1010920
display id: component1010920
version: 1
top level: BBa_I3001
definition: OmpR
access: public

BBa_B0012
type: Component
persistent identity: component1010852
display id: component1010852
version: 1
top level: BBa_I3001
definition: BBa_B0012
access: public

BBa_B0012
type: Component
persistent identity: component1010900
display id: component1010900
version: 1
top level: BBa_I3001
definition: BBa_B0012
access: public

BBa_R1075
type: Component
persistent identity: component1010768
display id: component1010768
version: 1
top level: BBa_I3001
definition: const.
access: public

BBa_B0010
type: Component
persistent identity: component1010890
display id: component1010890
version: 1
top level: BBa_I3001
definition: BBa_B0010
access: public

BBa_B0012
type: Component
persistent identity: component1010757
display id: component1010757
version: 1
top level: BBa_I3001
definition: BBa_B0012
access: public

BBa_R0080
type: Component
persistent identity: component1010974
display id: component1010974
version: 1
top level: BBa_I3001
definition: AraC
access: public

BBa_B0010
type: Component
persistent identity: component1010747
display id: component1010747
version: 1
top level: BBa_I3001
definition: BBa_B0010
access: public

BBa_B0012
type: Component
persistent identity: component1010951
display id: component1010951
version: 1
top level: BBa_I3001
definition: BBa_B0012
access: public

BBa_C0051
type: Component
persistent identity: component1010741
display id: component1010741
version: 1
top level: BBa_I3001
definition: cI lam
access: public

BBa_B0010
type: Component
persistent identity: component1010941
display id: component1010941
version: 1
top level: BBa_I3001
definition: BBa_B0010
access: public

BBa_B0012
type: Component
persistent identity: component1010800
display id: component1010800
version: 1
top level: BBa_I3001
definition: BBa_B0012
access: public

BBa_C0080
type: Component
persistent identity: component1010935
display id: component1010935
version: 1
top level: BBa_I3001
definition: araC
access: public

BBa_B0010
type: Component
persistent identity: component1010790
display id: component1010790
version: 1
top level: BBa_I3001
definition: BBa_B0010
access: public

BBa_C0012
type: Component
persistent identity: component1010783
display id: component1010783
version: 1
top level: BBa_I3001
definition: lacI
access: public

BBa_B0034
type: Component
persistent identity: component1010773
display id: component1010773
version: 1
top level: BBa_I3001
definition: BBa_B0034
access: public

BBa_C0051
type: Component
persistent identity: component1010689
display id: component1010689
version: 1
top level: BBa_I3001
definition: cI lam
access: public

BBa_B0034
type: Component
persistent identity: component1010679
display id: component1010679
version: 1
top level: BBa_I3001
definition: BBa_B0034
access: public

BBa_R0062
type: Component
persistent identity: component1010671
display id: component1010671
version: 1
top level: BBa_I3001
definition: lux pR
access: public

BBa_B0012
type: Component
persistent identity: component1010656
display id: component1010656
version: 1
top level: BBa_I3001
definition: BBa_B0012
access: public

BBa_B0034
type: Component
persistent identity: component1010731
display id: component1010731
version: 1
top level: BBa_I3001
definition: BBa_B0034
access: public

BBa_R0050
type: Component
persistent identity: component1010721
display id: component1010721
version: 1
top level: BBa_I3001
definition: cI HK022
access: public

BBa_B0012
type: Component
persistent identity: component1010705
display id: component1010705
version: 1
top level: BBa_I3001
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1010695
display id: component1010695
version: 1
top level: BBa_I3001
definition: BBa_B0010
access: public

BBa_B0012
type: Component
persistent identity: component1010597
display id: component1010597
version: 1
top level: BBa_I3001
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1010587
display id: component1010587
version: 1
top level: BBa_I3001
definition: BBa_B0010
access: public

BBa_E0022
type: Component
persistent identity: component1010579
display id: component1010579
version: 1
top level: BBa_I3001
definition: ECFP
access: public

BBa_B0034
type: Component
persistent identity: component1010567
display id: component1010567
version: 1
top level: BBa_I3001
definition: BBa_B0034
access: public

BBa_B0010
type: Component
persistent identity: component1010646
display id: component1010646
version: 1
top level: BBa_I3001
definition: BBa_B0010
access: public

BBa_C0062
type: Component
persistent identity: component1010638
display id: component1010638
version: 1
top level: BBa_I3001
definition: luxr
access: public

BBa_B0034
type: Component
persistent identity: component1010623
display id: component1010623
version: 1
top level: BBa_I3001
definition: BBa_B0034
access: public

BBa_R0063
type: Component
persistent identity: component1010614
display id: component1010614
version: 1
top level: BBa_I3001
definition: lux pL
access: public

BBa_R0051
type: Component
persistent identity: component1010503
display id: component1010503
version: 1
top level: BBa_I3001
definition: cI lam
access: public

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation1010503
display id: annotation1010503
version: 1
top level: BBa_I3001
location: https://synbiohub.org/public/igem/BBa_I3001/annotation1010503/range1010503/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_I3001/annotation1010503/range1010503/1
type: Range
persistent identity: range1010503
display id: range1010503
version: 1
top level: BBa_I3001
direction: direction/forward
start: 1
end: 49
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1010511
display id: annotation1010511
version: 1
top level: BBa_I3001
location: https://synbiohub.org/public/igem/BBa_I3001/annotation1010511/range1010511/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I3001/annotation1010511/range1010511/1
type: Range
persistent identity: range1010511
display id: range1010511
version: 1
top level: BBa_I3001
direction: direction/forward
start: 58
end: 69
orientation: inline

BBa_C0050
type: SequenceAnnotation
persistent identity: annotation1010526
display id: annotation1010526
version: 1
top level: BBa_I3001
location: https://synbiohub.org/public/igem/BBa_I3001/annotation1010526/range1010526/1
component: BBa_C0050

https://synbiohub.org/public/igem/BBa_I3001/annotation1010526/range1010526/1
type: Range
persistent identity: range1010526
display id: range1010526
version: 1
top level: BBa_I3001
direction: direction/forward
start: 76
end: 819
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1010534
display id: annotation1010534
version: 1
top level: BBa_I3001
location: https://synbiohub.org/public/igem/BBa_I3001/annotation1010534/range1010534/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I3001/annotation1010534/range1010534/1
type: Range
persistent identity: range1010534
display id: range1010534
version: 1
top level: BBa_I3001
direction: direction/forward
start: 853
end: 864
orientation: inline

BBa_C0083
type: SequenceAnnotation
persistent identity: annotation1010561
display id: annotation1010561
version: 1
top level: BBa_I3001
location: https://synbiohub.org/public/igem/BBa_I3001/annotation1010561/range1010561/1
component: BBa_C0083

https://synbiohub.org/public/igem/BBa_I3001/annotation1010561/range1010561/1
type: Range
persistent identity: range1010561
display id: range1010561
version: 1
top level: BBa_I3001
direction: direction/forward
start: 871
end: 2388
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1010567
display id: annotation1010567
version: 1
top level: BBa_I3001
location: https://synbiohub.org/public/igem/BBa_I3001/annotation1010567/range1010567/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I3001/annotation1010567/range1010567/1
type: Range
persistent identity: range1010567
display id: range1010567
version: 1
top level: BBa_I3001
direction: direction/forward
start: 2422
end: 2433
orientation: inline

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation1010579
display id: annotation1010579
version: 1
top level: BBa_I3001
location: https://synbiohub.org/public/igem/BBa_I3001/annotation1010579/range1010579/1
component: BBa_E0022

https://synbiohub.org/public/igem/BBa_I3001/annotation1010579/range1010579/1
type: Range
persistent identity: range1010579
display id: range1010579
version: 1
top level: BBa_I3001
direction: direction/forward
start: 2440
end: 3201
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1010587
display id: annotation1010587
version: 1
top level: BBa_I3001
location: https://synbiohub.org/public/igem/BBa_I3001/annotation1010587/range1010587/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I3001/annotation1010587/range1010587/1
type: Range
persistent identity: range1010587
display id: range1010587
version: 1
top level: BBa_I3001
direction: direction/forward
start: 3210
end: 3289
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1010597
display id: annotation1010597
version: 1
top level: BBa_I3001
location: https://synbiohub.org/public/igem/BBa_I3001/annotation1010597/range1010597/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I3001/annotation1010597/range1010597/1
type: Range
persistent identity: range1010597
display id: range1010597
version: 1
top level: BBa_I3001
direction: direction/forward
start: 3298
end: 3338
orientation: inline

BBa_R0063
type: SequenceAnnotation
persistent identity: annotation1010614
display id: annotation1010614
version: 1
top level: BBa_I3001
location: https://synbiohub.org/public/igem/BBa_I3001/annotation1010614/range1010614/1
component: BBa_R0063

https://synbiohub.org/public/igem/BBa_I3001/annotation1010614/range1010614/1
type: Range
persistent identity: range1010614
display id: range1010614
version: 1
top level: BBa_I3001
direction: direction/forward
start: 3347
end: 3497
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1010623
display id: annotation1010623
version: 1
top level: BBa_I3001
location: https://synbiohub.org/public/igem/BBa_I3001/annotation1010623/range1010623/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I3001/annotation1010623/range1010623/1
type: Range
persistent identity: range1010623
display id: range1010623
version: 1
top level: BBa_I3001
direction: direction/forward
start: 3506
end: 3517
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation1010638
display id: annotation1010638
version: 1
top level: BBa_I3001
location: https://synbiohub.org/public/igem/BBa_I3001/annotation1010638/range1010638/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_I3001/annotation1010638/range1010638/1
type: Range
persistent identity: range1010638
display id: range1010638
version: 1
top level: BBa_I3001
direction: direction/forward
start: 3524
end: 4279
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1010646
display id: annotation1010646
version: 1
top level: BBa_I3001
location: https://synbiohub.org/public/igem/BBa_I3001/annotation1010646/range1010646/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I3001/annotation1010646/range1010646/1
type: Range
persistent identity: range1010646
display id: range1010646
version: 1
top level: BBa_I3001
direction: direction/forward
start: 4313
end: 4392
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1010656
display id: annotation1010656
version: 1
top level: BBa_I3001
location: https://synbiohub.org/public/igem/BBa_I3001/annotation1010656/range1010656/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I3001/annotation1010656/range1010656/1
type: Range
persistent identity: range1010656
display id: range1010656
version: 1
top level: BBa_I3001
direction: direction/forward
start: 4401
end: 4441
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation1010671
display id: annotation1010671
version: 1
top level: BBa_I3001
location: https://synbiohub.org/public/igem/BBa_I3001/annotation1010671/range1010671/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_I3001/annotation1010671/range1010671/1
type: Range
persistent identity: range1010671
display id: range1010671
version: 1
top level: BBa_I3001
direction: direction/forward
start: 4450
end: 4504
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1010679
display id: annotation1010679
version: 1
top level: BBa_I3001
location: https://synbiohub.org/public/igem/BBa_I3001/annotation1010679/range1010679/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I3001/annotation1010679/range1010679/1
type: Range
persistent identity: range1010679
display id: range1010679
version: 1
top level: BBa_I3001
direction: direction/forward
start: 4513
end: 4524
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation1010689
display id: annotation1010689
version: 1
top level: BBa_I3001
location: https://synbiohub.org/public/igem/BBa_I3001/annotation1010689/range1010689/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_I3001/annotation1010689/range1010689/1
type: Range
persistent identity: range1010689
display id: range1010689
version: 1
top level: BBa_I3001
direction: direction/forward
start: 4531
end: 5280
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1010695
display id: annotation1010695
version: 1
top level: BBa_I3001
location: https://synbiohub.org/public/igem/BBa_I3001/annotation1010695/range1010695/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I3001/annotation1010695/range1010695/1
type: Range
persistent identity: range1010695
display id: range1010695
version: 1
top level: BBa_I3001
direction: direction/forward
start: 5314
end: 5393
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1010705
display id: annotation1010705
version: 1
top level: BBa_I3001
location: https://synbiohub.org/public/igem/BBa_I3001/annotation1010705/range1010705/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I3001/annotation1010705/range1010705/1
type: Range
persistent identity: range1010705
display id: range1010705
version: 1
top level: BBa_I3001
direction: direction/forward
start: 5402
end: 5442
orientation: inline

BBa_R0050
type: SequenceAnnotation
persistent identity: annotation1010721
display id: annotation1010721
version: 1
top level: BBa_I3001
location: https://synbiohub.org/public/igem/BBa_I3001/annotation1010721/range1010721/1
component: BBa_R0050

https://synbiohub.org/public/igem/BBa_I3001/annotation1010721/range1010721/1
type: Range
persistent identity: range1010721
display id: range1010721
version: 1
top level: BBa_I3001
direction: direction/forward
start: 5451
end: 5505
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1010731
display id: annotation1010731
version: 1
top level: BBa_I3001
location: https://synbiohub.org/public/igem/BBa_I3001/annotation1010731/range1010731/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I3001/annotation1010731/range1010731/1
type: Range
persistent identity: range1010731
display id: range1010731
version: 1
top level: BBa_I3001
direction: direction/forward
start: 5514
end: 5525
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation1010741
display id: annotation1010741
version: 1
top level: BBa_I3001
location: https://synbiohub.org/public/igem/BBa_I3001/annotation1010741/range1010741/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_I3001/annotation1010741/range1010741/1
type: Range
persistent identity: range1010741
display id: range1010741
version: 1
top level: BBa_I3001
direction: direction/forward
start: 5532
end: 6281
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1010747
display id: annotation1010747
version: 1
top level: BBa_I3001
location: https://synbiohub.org/public/igem/BBa_I3001/annotation1010747/range1010747/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I3001/annotation1010747/range1010747/1
type: Range
persistent identity: range1010747
display id: range1010747
version: 1
top level: BBa_I3001
direction: direction/forward
start: 6315
end: 6394
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1010757
display id: annotation1010757
version: 1
top level: BBa_I3001
location: https://synbiohub.org/public/igem/BBa_I3001/annotation1010757/range1010757/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I3001/annotation1010757/range1010757/1
type: Range
persistent identity: range1010757
display id: range1010757
version: 1
top level: BBa_I3001
direction: direction/forward
start: 6403
end: 6443
orientation: inline

BBa_R1075
type: SequenceAnnotation
persistent identity: annotation1010768
display id: annotation1010768
version: 1
top level: BBa_I3001
location: https://synbiohub.org/public/igem/BBa_I3001/annotation1010768/range1010768/1
component: BBa_R1075

https://synbiohub.org/public/igem/BBa_I3001/annotation1010768/range1010768/1
type: Range
persistent identity: range1010768
display id: range1010768
version: 1
top level: BBa_I3001
direction: direction/forward
start: 6452
end: 6500
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1010773
display id: annotation1010773
version: 1
top level: BBa_I3001
location: https://synbiohub.org/public/igem/BBa_I3001/annotation1010773/range1010773/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I3001/annotation1010773/range1010773/1
type: Range
persistent identity: range1010773
display id: range1010773
version: 1
top level: BBa_I3001
direction: direction/forward
start: 6509
end: 6520
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation1010783
display id: annotation1010783
version: 1
top level: BBa_I3001
location: https://synbiohub.org/public/igem/BBa_I3001/annotation1010783/range1010783/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_I3001/annotation1010783/range1010783/1
type: Range
persistent identity: range1010783
display id: range1010783
version: 1
top level: BBa_I3001
direction: direction/forward
start: 6527
end: 7654
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1010790
display id: annotation1010790
version: 1
top level: BBa_I3001
location: https://synbiohub.org/public/igem/BBa_I3001/annotation1010790/range1010790/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I3001/annotation1010790/range1010790/1
type: Range
persistent identity: range1010790
display id: range1010790
version: 1
top level: BBa_I3001
direction: direction/forward
start: 7688
end: 7767
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1010800
display id: annotation1010800
version: 1
top level: BBa_I3001
location: https://synbiohub.org/public/igem/BBa_I3001/annotation1010800/range1010800/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I3001/annotation1010800/range1010800/1
type: Range
persistent identity: range1010800
display id: range1010800
version: 1
top level: BBa_I3001
direction: direction/forward
start: 7776
end: 7816
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1010816
display id: annotation1010816
version: 1
top level: BBa_I3001
location: https://synbiohub.org/public/igem/BBa_I3001/annotation1010816/range1010816/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_I3001/annotation1010816/range1010816/1
type: Range
persistent identity: range1010816
display id: range1010816
version: 1
top level: BBa_I3001
direction: direction/forward
start: 7825
end: 8024
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1010826
display id: annotation1010826
version: 1
top level: BBa_I3001
location: https://synbiohub.org/public/igem/BBa_I3001/annotation1010826/range1010826/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I3001/annotation1010826/range1010826/1
type: Range
persistent identity: range1010826
display id: range1010826
version: 1
top level: BBa_I3001
direction: direction/forward
start: 8033
end: 8044
orientation: inline

BBa_C0080
type: SequenceAnnotation
persistent identity: annotation1010836
display id: annotation1010836
version: 1
top level: BBa_I3001
location: https://synbiohub.org/public/igem/BBa_I3001/annotation1010836/range1010836/1
component: BBa_C0080

https://synbiohub.org/public/igem/BBa_I3001/annotation1010836/range1010836/1
type: Range
persistent identity: range1010836
display id: range1010836
version: 1
top level: BBa_I3001
direction: direction/forward
start: 8051
end: 8965
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1010842
display id: annotation1010842
version: 1
top level: BBa_I3001
location: https://synbiohub.org/public/igem/BBa_I3001/annotation1010842/range1010842/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I3001/annotation1010842/range1010842/1
type: Range
persistent identity: range1010842
display id: range1010842
version: 1
top level: BBa_I3001
direction: direction/forward
start: 8999
end: 9078
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1010852
display id: annotation1010852
version: 1
top level: BBa_I3001
location: https://synbiohub.org/public/igem/BBa_I3001/annotation1010852/range1010852/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I3001/annotation1010852/range1010852/1
type: Range
persistent identity: range1010852
display id: range1010852
version: 1
top level: BBa_I3001
direction: direction/forward
start: 9087
end: 9127
orientation: inline

BBa_R1075
type: SequenceAnnotation
persistent identity: annotation1010863
display id: annotation1010863
version: 1
top level: BBa_I3001
location: https://synbiohub.org/public/igem/BBa_I3001/annotation1010863/range1010863/1
component: BBa_R1075

https://synbiohub.org/public/igem/BBa_I3001/annotation1010863/range1010863/1
type: Range
persistent identity: range1010863
display id: range1010863
version: 1
top level: BBa_I3001
direction: direction/forward
start: 9136
end: 9184
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1010868
display id: annotation1010868
version: 1
top level: BBa_I3001
location: https://synbiohub.org/public/igem/BBa_I3001/annotation1010868/range1010868/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I3001/annotation1010868/range1010868/1
type: Range
persistent identity: range1010868
display id: range1010868
version: 1
top level: BBa_I3001
direction: direction/forward
start: 9193
end: 9204
orientation: inline

BBa_C0082
type: SequenceAnnotation
persistent identity: annotation1010884
display id: annotation1010884
version: 1
top level: BBa_I3001
location: https://synbiohub.org/public/igem/BBa_I3001/annotation1010884/range1010884/1
component: BBa_C0082

https://synbiohub.org/public/igem/BBa_I3001/annotation1010884/range1010884/1
type: Range
persistent identity: range1010884
display id: range1010884
version: 1
top level: BBa_I3001
direction: direction/forward
start: 9211
end: 10701
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1010890
display id: annotation1010890
version: 1
top level: BBa_I3001
location: https://synbiohub.org/public/igem/BBa_I3001/annotation1010890/range1010890/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I3001/annotation1010890/range1010890/1
type: Range
persistent identity: range1010890
display id: range1010890
version: 1
top level: BBa_I3001
direction: direction/forward
start: 10735
end: 10814
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1010900
display id: annotation1010900
version: 1
top level: BBa_I3001
location: https://synbiohub.org/public/igem/BBa_I3001/annotation1010900/range1010900/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I3001/annotation1010900/range1010900/1
type: Range
persistent identity: range1010900
display id: range1010900
version: 1
top level: BBa_I3001
direction: direction/forward
start: 10823
end: 10863
orientation: inline

BBa_R0082
type: SequenceAnnotation
persistent identity: annotation1010920
display id: annotation1010920
version: 1
top level: BBa_I3001
location: https://synbiohub.org/public/igem/BBa_I3001/annotation1010920/range1010920/1
component: BBa_R0082

https://synbiohub.org/public/igem/BBa_I3001/annotation1010920/range1010920/1
type: Range
persistent identity: range1010920
display id: range1010920
version: 1
top level: BBa_I3001
direction: direction/forward
start: 10872
end: 10979
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1010925
display id: annotation1010925
version: 1
top level: BBa_I3001
location: https://synbiohub.org/public/igem/BBa_I3001/annotation1010925/range1010925/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I3001/annotation1010925/range1010925/1
type: Range
persistent identity: range1010925
display id: range1010925
version: 1
top level: BBa_I3001
direction: direction/forward
start: 10988
end: 10999
orientation: inline

BBa_C0080
type: SequenceAnnotation
persistent identity: annotation1010935
display id: annotation1010935
version: 1
top level: BBa_I3001
location: https://synbiohub.org/public/igem/BBa_I3001/annotation1010935/range1010935/1
component: BBa_C0080

https://synbiohub.org/public/igem/BBa_I3001/annotation1010935/range1010935/1
type: Range
persistent identity: range1010935
display id: range1010935
version: 1
top level: BBa_I3001
direction: direction/forward
start: 11006
end: 11920
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1010941
display id: annotation1010941
version: 1
top level: BBa_I3001
location: https://synbiohub.org/public/igem/BBa_I3001/annotation1010941/range1010941/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I3001/annotation1010941/range1010941/1
type: Range
persistent identity: range1010941
display id: range1010941
version: 1
top level: BBa_I3001
direction: direction/forward
start: 11954
end: 12033
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1010951
display id: annotation1010951
version: 1
top level: BBa_I3001
location: https://synbiohub.org/public/igem/BBa_I3001/annotation1010951/range1010951/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I3001/annotation1010951/range1010951/1
type: Range
persistent identity: range1010951
display id: range1010951
version: 1
top level: BBa_I3001
direction: direction/forward
start: 12042
end: 12082
orientation: inline

BBa_R0080
type: SequenceAnnotation
persistent identity: annotation1010974
display id: annotation1010974
version: 1
top level: BBa_I3001
location: https://synbiohub.org/public/igem/BBa_I3001/annotation1010974/range1010974/1
component: BBa_R0080

https://synbiohub.org/public/igem/BBa_I3001/annotation1010974/range1010974/1
type: Range
persistent identity: range1010974
display id: range1010974
version: 1
top level: BBa_I3001
direction: direction/forward
start: 12091
end: 12239
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1010979
display id: annotation1010979
version: 1
top level: BBa_I3001
location: https://synbiohub.org/public/igem/BBa_I3001/annotation1010979/range1010979/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I3001/annotation1010979/range1010979/1
type: Range
persistent identity: range1010979
display id: range1010979
version: 1
top level: BBa_I3001
direction: direction/forward
start: 12248
end: 12259
orientation: inline

BBa_C0050
type: SequenceAnnotation
persistent identity: annotation1010994
display id: annotation1010994
version: 1
top level: BBa_I3001
location: https://synbiohub.org/public/igem/BBa_I3001/annotation1010994/range1010994/1
component: BBa_C0050

https://synbiohub.org/public/igem/BBa_I3001/annotation1010994/range1010994/1
type: Range
persistent identity: range1010994
display id: range1010994
version: 1
top level: BBa_I3001
direction: direction/forward
start: 12266
end: 13009
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1011002
display id: annotation1011002
version: 1
top level: BBa_I3001
location: https://synbiohub.org/public/igem/BBa_I3001/annotation1011002/range1011002/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I3001/annotation1011002/range1011002/1
type: Range
persistent identity: range1011002
display id: range1011002
version: 1
top level: BBa_I3001
direction: direction/forward
start: 13043
end: 13122
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1011012
display id: annotation1011012
version: 1
top level: BBa_I3001
location: https://synbiohub.org/public/igem/BBa_I3001/annotation1011012/range1011012/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I3001/annotation1011012/range1011012/1
type: Range
persistent identity: range1011012
display id: range1011012
version: 1
top level: BBa_I3001
direction: direction/forward
start: 13131
end: 13171
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation1011027
display id: annotation1011027
version: 1
top level: BBa_I3001
location: https://synbiohub.org/public/igem/BBa_I3001/annotation1011027/range1011027/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_I3001/annotation1011027/range1011027/1
type: Range
persistent identity: range1011027
display id: range1011027
version: 1
top level: BBa_I3001
direction: direction/forward
start: 13180
end: 13234
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1011035
display id: annotation1011035
version: 1
top level: BBa_I3001
location: https://synbiohub.org/public/igem/BBa_I3001/annotation1011035/range1011035/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I3001/annotation1011035/range1011035/1
type: Range
persistent identity: range1011035
display id: range1011035
version: 1
top level: BBa_I3001
direction: direction/forward
start: 13243
end: 13254
orientation: inline

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation1011045
display id: annotation1011045
version: 1
top level: BBa_I3001
location: https://synbiohub.org/public/igem/BBa_I3001/annotation1011045/range1011045/1
component: BBa_C0061

https://synbiohub.org/public/igem/BBa_I3001/annotation1011045/range1011045/1
type: Range
persistent identity: range1011045
display id: range1011045
version: 1
top level: BBa_I3001
direction: direction/forward
start: 13261
end: 13878
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1011052
display id: annotation1011052
version: 1
top level: BBa_I3001
location: https://synbiohub.org/public/igem/BBa_I3001/annotation1011052/range1011052/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I3001/annotation1011052/range1011052/1
type: Range
persistent identity: range1011052
display id: range1011052
version: 1
top level: BBa_I3001
direction: direction/forward
start: 13912
end: 13991
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1011062
display id: annotation1011062
version: 1
top level: BBa_I3001
location: https://synbiohub.org/public/igem/BBa_I3001/annotation1011062/range1011062/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I3001/annotation1011062/range1011062/1
type: Range
persistent identity: range1011062
display id: range1011062
version: 1
top level: BBa_I3001
direction: direction/forward
start: 14000
end: 14040
orientation: inline

tar-envZ
type: ComponentDefinition
persistent identity: BBa C0082
display id: BBa_C0082
version: 1
was generated by: iGEM to SBOL conversion
description: Receptor, tar-envZ
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:24Z
mutable provenance: NC_000913 E. coli K12
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding sequence for Tar-EnvZ fusion protein. Tar is the bacterial chemotaxis receptor for aspartate. EnvZ is an osmosensor kinase, which, through phosphorylation of OmpR, regulates transcription of porin genes. This fusion protein combines the extracellular and transmembrane domains of Tar with the intracellular domain of EnvZ. The fusion converts chemical binding of aspartate to production of transcription factors.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: A silent point mutation was made at base 423 (A -> C) to eliminate an XbaI restriction enzyme site. The mutation was designed to match codons with similar frequencies of usage in E. coli.
star: false
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Tar (front)
sequence annotation: A
sequence annotation: Envz (back)
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0082_sequence/1

Tar (front)
type: SequenceAnnotation
persistent identity: annotation308352
display id: annotation308352
version: 1
top level: tar-envZ
location: https://synbiohub.org/public/igem/BBa_C0082/annotation308352/range308352/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0082/annotation308352/range308352/1
type: Range
persistent identity: range308352
display id: range308352
version: 1
top level: tar-envZ
direction: direction/forward
start: 1
end: 765
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation300943
display id: annotation300943
version: 1
top level: tar-envZ
location: https://synbiohub.org/public/igem/BBa_C0082/annotation300943/range300943/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0082/annotation300943/range300943/1
type: Range
persistent identity: range300943
display id: range300943
version: 1
top level: tar-envZ
direction: direction/forward
start: 423
end: 424
orientation: inline

Envz (back)
type: SequenceAnnotation
persistent identity: annotation308360
display id: annotation308360
version: 1
top level: tar-envZ
location: https://synbiohub.org/public/igem/BBa_C0082/annotation308360/range308360/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0082/annotation308360/range308360/1
type: Range
persistent identity: range308360
display id: range308360
version: 1
top level: tar-envZ
direction: direction/forward
start: 772
end: 1452
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation301146
display id: annotation301146
version: 1
top level: tar-envZ
location: https://synbiohub.org/public/igem/BBa_C0082/annotation301146/range301146/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0082/annotation301146/range301146/1
type: Range
persistent identity: range301146
display id: range301146
version: 1
top level: tar-envZ
direction: direction/forward
start: 1453
end: 1485
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213997
display id: annotation2213997
version: 1
top level: tar-envZ
location: https://synbiohub.org/public/igem/BBa_C0082/annotation2213997/range2213997/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0082/annotation2213997/range2213997/1
type: Range
persistent identity: range2213997
display id: range2213997
version: 1
top level: tar-envZ
direction: direction/forward
start: 1492
end: 1516
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

araC
type: ComponentDefinition
persistent identity: BBa C0080
display id: BBa_C0080
version: 1
was generated by: iGEM to SBOL conversion
description: araC arabinose operon regulatory protein (repressor/activator) from E. coli (+LVA)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:24Z
mutable provenance: GenBank: NC_002655 (www.ncbi.nlm.nih.gov)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: coding region for the araC gene, used to make araC protein for use in positive or negative regulation of TIPs output (see also R0080, R0081)
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Sara Neves (Fighting Darwins)
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: araC
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0080_sequence/1

araC
type: SequenceAnnotation
persistent identity: annotation308191
display id: annotation308191
version: 1
top level: araC
location: https://synbiohub.org/public/igem/BBa_C0080/annotation308191/range308191/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0080/annotation308191/range308191/1
type: Range
persistent identity: range308191
display id: range308191
version: 1
top level: araC
direction: direction/forward
start: 1
end: 876
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation308196
display id: annotation308196
version: 1
top level: araC
location: https://synbiohub.org/public/igem/BBa_C0080/annotation308196/range308196/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0080/annotation308196/range308196/1
type: Range
persistent identity: range308196
display id: range308196
version: 1
top level: araC
direction: direction/forward
start: 877
end: 909
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214004
display id: annotation2214004
version: 1
top level: araC
location: https://synbiohub.org/public/igem/BBa_C0080/annotation2214004/range2214004/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0080/annotation2214004/range2214004/1
type: Range
persistent identity: range2214004
display id: range2214004
version: 1
top level: araC
direction: direction/forward
start: 916
end: 940
orientation: inline

cI HK022
type: ComponentDefinition
persistent identity: BBa C0050
display id: BBa_C0050
version: 1
was generated by: iGEM to SBOL conversion
description: cI repressor from phage HK022 (+LVA?)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Bacteriophage HK022.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the HK022 bacteriophage cI protein. cI binds to the HK022 pR regulator (BBa_R0050). It represses transcription of the protein encoded by the sequence 3' to the pR region. This coding sequence does not contain a RBS.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Carlson NG, Little JW. Highly cooperative DNA binding by the coliphage HK022 repressor. J Mol Biol. 1993 Apr 20;230(4):1108-30. PMID: 8487297. Mao C, Little JW. Mutations affecting cooperative DNA binding of phage HK022 CI repressor. J Mol Biol. 1998 May 29;279(1):31-48. PMID: 9636698. References (unparsed) here: Carlson NG, Little JW. Highly cooperative DNA binding by the coliphage HK022 repressor. J Mol Biol. 1993 Apr 20;230(4):1108-30. PMID: 8487297. Mao C, Little JW. Mutations affecting cooperative DNA binding of phage HK022 CI repressor. J Mol Biol. 1998 May 29;279(1):31-48. PMID: 9636698. Derived from <genbank>STHK022N</genbank>. Response from John Little (Arizona) regarding the start of HK022 cI From: <jlittle@email.arizona.edu> Date: Tue Jan 21, 2003 4:39:21 PM US/Eastern To: "Drew Endy" <endy@MIT.EDU> Subject: RE: hk022 cI start (naive question)? Hello Drew and Michael, I seriously doubt that the extra 27 aa are part of CI. It doesn't make sense in terms of the biology, for sure. In any case, the protein we characterized starts where Carlson and Little stated; as I recall, oR3 partially overlaps the start of cI. I have a vague memory of some possibly interesting biology, having to do with multicopy plasmids. I don't recall the findings themselves. Anyway, one possible explanation was that this extra N-terminal addition was made (e.g. from the pRE promoter, or from a message that arose from transcription around the entire plasmid), making a protein with altered functions. We never followed it up. Good luck John -- Original Message -- Date: Sun, 19 Jan 2003 15:26:26 -0500 Subject: hk022 cI start (naive question)? Cc: elowitm@rockefeller.edu To: jlittle@u.arizona.edu From: Drew Endy <endy@MIT.EDU> Hi John, I'm sitting here with Michael Elowitz and we're working through the sequence for HK022 cI. We noticed that the annotation from NC_002166 includes an "extra" 81 base pairs upstream of what we thought of as the actual start. It look…
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: BBa_C0050
sequence annotation: OR3 partial
sequence annotation: cI HK022
sequence annotation: SsrA
sequence annotation: 2
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0050_sequence/1

BBa_C0050
type: SequenceAnnotation
persistent identity: annotation7033
display id: annotation7033
version: 1
top level: cI HK022
location: https://synbiohub.org/public/igem/BBa_C0050/annotation7033/range7033/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0050/annotation7033/range7033/1
type: Range
persistent identity: range7033
display id: range7033
version: 1
top level: cI HK022
direction: direction/forward
start: 1
end: 744
orientation: inline

OR3 partial
type: SequenceAnnotation
persistent identity: annotation1737
display id: annotation1737
version: 1
top level: cI HK022
location: https://synbiohub.org/public/igem/BBa_C0050/annotation1737/range1737/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_C0050/annotation1737/range1737/1
type: Range
persistent identity: range1737
display id: range1737
version: 1
top level: cI HK022
direction: direction/forward
start: 1
end: 8
orientation: inline

cI HK022
type: SequenceAnnotation
persistent identity: annotation1735
display id: annotation1735
version: 1
top level: cI HK022
location: https://synbiohub.org/public/igem/BBa_C0050/annotation1735/range1735/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0050/annotation1735/range1735/1
type: Range
persistent identity: range1735
display id: range1735
version: 1
top level: cI HK022
direction: direction/forward
start: 1
end: 744
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation1736
display id: annotation1736
version: 1
top level: cI HK022
location: https://synbiohub.org/public/igem/BBa_C0050/annotation1736/range1736/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0050/annotation1736/range1736/1
type: Range
persistent identity: range1736
display id: range1736
version: 1
top level: cI HK022
direction: direction/forward
start: 706
end: 738
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation1734
display id: annotation1734
version: 1
top level: cI HK022
location: https://synbiohub.org/public/igem/BBa_C0050/annotation1734/range1734/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_C0050/annotation1734/range1734/1
type: Range
persistent identity: range1734
display id: range1734
version: 1
top level: cI HK022
direction: direction/forward
start: 739
end: 744
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213990
display id: annotation2213990
version: 1
top level: cI HK022
location: https://synbiohub.org/public/igem/BBa_C0050/annotation2213990/range2213990/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0050/annotation2213990/range2213990/1
type: Range
persistent identity: range2213990
display id: range2213990
version: 1
top level: cI HK022
direction: direction/forward
start: 745
end: 769
orientation: inline

aspA
type: ComponentDefinition
persistent identity: BBa C0083
display id: BBa_C0083
version: 1
was generated by: iGEM to SBOL conversion
description: aspartate ammonia-lyase
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:24Z
mutable provenance: AE000486 E. coli K12
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding sequence for AspA enzyme. AspA aminates fumarate to make aspartate. Aspartate can be used as a bacterial chemotaxis signal.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The start codon was changed from GTG to ATG to match BioBricks standards. Five silent mutations were introduced to eliminate EcoRI and PstI restriction enzyme sites. EcoRI sites were eliminated at bases 588 and 657 with C -> T mutations. PstI sites were eliminated at bases 624, 999, and 1125 with G -> A mutations. The mutations were designed to match codons with similar frequencies of usage in E. coli.
star: true
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: AspA
sequence annotation: G
sequence annotation: C
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0083_sequence/1

AspA
type: SequenceAnnotation
persistent identity: annotation300981
display id: annotation300981
version: 1
top level: aspA
location: https://synbiohub.org/public/igem/BBa_C0083/annotation300981/range300981/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0083/annotation300981/range300981/1
type: Range
persistent identity: range300981
display id: range300981
version: 1
top level: aspA
direction: direction/forward
start: 1
end: 1479
orientation: inline

G
type: SequenceAnnotation
persistent identity: annotation300982
display id: annotation300982
version: 1
top level: aspA
location: https://synbiohub.org/public/igem/BBa_C0083/annotation300982/range300982/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0083/annotation300982/range300982/1
type: Range
persistent identity: range300982
display id: range300982
version: 1
top level: aspA
direction: direction/forward
start: 1
end: 1
orientation: inline

C
type: SequenceAnnotation
persistent identity: annotation300983
display id: annotation300983
version: 1
top level: aspA
location: https://synbiohub.org/public/igem/BBa_C0083/annotation300983/range300983/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0083/annotation300983/range300983/1
type: Range
persistent identity: range300983
display id: range300983
version: 1
top level: aspA
direction: direction/forward
start: 588
end: 588
orientation: inline

G
type: SequenceAnnotation
persistent identity: annotation300984
display id: annotation300984
version: 1
top level: aspA
location: https://synbiohub.org/public/igem/BBa_C0083/annotation300984/range300984/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0083/annotation300984/range300984/1
type: Range
persistent identity: range300984
display id: range300984
version: 1
top level: aspA
direction: direction/forward
start: 624
end: 624
orientation: inline

C
type: SequenceAnnotation
persistent identity: annotation300986
display id: annotation300986
version: 1
top level: aspA
location: https://synbiohub.org/public/igem/BBa_C0083/annotation300986/range300986/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0083/annotation300986/range300986/1
type: Range
persistent identity: range300986
display id: range300986
version: 1
top level: aspA
direction: direction/forward
start: 657
end: 657
orientation: inline

G
type: SequenceAnnotation
persistent identity: annotation300987
display id: annotation300987
version: 1
top level: aspA
location: https://synbiohub.org/public/igem/BBa_C0083/annotation300987/range300987/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0083/annotation300987/range300987/1
type: Range
persistent identity: range300987
display id: range300987
version: 1
top level: aspA
direction: direction/forward
start: 999
end: 999
orientation: inline

G
type: SequenceAnnotation
persistent identity: annotation300988
display id: annotation300988
version: 1
top level: aspA
location: https://synbiohub.org/public/igem/BBa_C0083/annotation300988/range300988/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_C0083/annotation300988/range300988/1
type: Range
persistent identity: range300988
display id: range300988
version: 1
top level: aspA
direction: direction/forward
start: 1125
end: 1125
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation300993
display id: annotation300993
version: 1
top level: aspA
location: https://synbiohub.org/public/igem/BBa_C0083/annotation300993/range300993/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0083/annotation300993/range300993/1
type: Range
persistent identity: range300993
display id: range300993
version: 1
top level: aspA
direction: direction/forward
start: 1480
end: 1512
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213998
display id: annotation2213998
version: 1
top level: aspA
location: https://synbiohub.org/public/igem/BBa_C0083/annotation2213998/range2213998/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0083/annotation2213998/range2213998/1
type: Range
persistent identity: range2213998
display id: range2213998
version: 1
top level: aspA
direction: direction/forward
start: 1519
end: 1543
orientation: inline

const.
type: ComponentDefinition
persistent identity: BBa R1075
display id: BBa_R1075
version: 1
was generated by: iGEM to SBOL conversion
description: Constitutive Promoter II
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: Gaal, Gourse, et al.
owned by: james
owned by: myers
mutable description: constitutive promoter which outputs .4 TIPs point mutants of wild type rrnB promoter P1
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Chris Walsh (Fighting Darwins)
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R1075_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation309024
display id: annotation309024
version: 1
top level: const.
location: https://synbiohub.org/public/igem/BBa_R1075/annotation309024/range309024/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R1075/annotation309024/range309024/1
type: Range
persistent identity: range309024
display id: range309024
version: 1
top level: const.
direction: direction/forward
start: 15
end: 20
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation309020
display id: annotation309020
version: 1
top level: const.
location: https://synbiohub.org/public/igem/BBa_R1075/annotation309020/range309020/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R1075/annotation309020/range309020/1
type: Range
persistent identity: range309020
display id: range309020
version: 1
top level: const.
direction: direction/forward
start: 35
end: 40
orientation: inline

OmpR
type: ComponentDefinition
persistent identity: BBa R0082
display id: BBa_R0082
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (OmpR, positive)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: NC_000193 E. coli K12
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The promoter includes the three OmpR binding sites: C1, C2, and C3. The promoter starts at -107 and goes up to the transcriptional start site at +1. An alternate version, BBa_R0083, cuts out the C2 and C3 sites. The efficacy of this promoter versus the truncated ompC upstream region (BBa_R0083) or the ompF upstream region (BBa_R0084) is currently unknown.
star: true
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: C1 OmpR
sequence annotation: C2 OmpR
sequence annotation: C3 OmpR
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0082_sequence/1

C1 OmpR
type: SequenceAnnotation
persistent identity: annotation301154
display id: annotation301154
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301154/range301154/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0082/annotation301154/range301154/1
type: Range
persistent identity: range301154
display id: range301154
version: 1
top level: OmpR
direction: direction/forward
start: 13
end: 30
orientation: inline

C2 OmpR
type: SequenceAnnotation
persistent identity: annotation301155
display id: annotation301155
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301155/range301155/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0082/annotation301155/range301155/1
type: Range
persistent identity: range301155
display id: range301155
version: 1
top level: OmpR
direction: direction/forward
start: 34
end: 51
orientation: inline

C3 OmpR
type: SequenceAnnotation
persistent identity: annotation301156
display id: annotation301156
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301156/range301156/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0082/annotation301156/range301156/1
type: Range
persistent identity: range301156
display id: range301156
version: 1
top level: OmpR
direction: direction/forward
start: 54
end: 71
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation301166
display id: annotation301166
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301166/range301166/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0082/annotation301166/range301166/1
type: Range
persistent identity: range301166
display id: range301166
version: 1
top level: OmpR
direction: direction/forward
start: 75
end: 80
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation301167
display id: annotation301167
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301167/range301167/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0082/annotation301167/range301167/1
type: Range
persistent identity: range301167
display id: range301167
version: 1
top level: OmpR
direction: direction/forward
start: 98
end: 103
orientation: inline

luxI
type: ComponentDefinition
persistent identity: BBa C0061
display id: BBa_C0061
version: 1
was generated by: iGEM to SBOL conversion
description: autoinducer synthetase for AHL
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: V. fischeri <genbank>AF170104</genbank>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Synthesizes 3OC6HSL, which binds to LuxR. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound HSL. This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: An LVA tail (sequence: AANDENYALVA) was added to increase protein degradation. .
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_C0061
sequence annotation: luxI
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0061_sequence/1

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation7038
display id: annotation7038
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation7038/range7038/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0061/annotation7038/range7038/1
type: Range
persistent identity: range7038
display id: range7038
version: 1
top level: luxI
direction: direction/forward
start: 1
end: 618
orientation: inline

luxI
type: SequenceAnnotation
persistent identity: annotation1761
display id: annotation1761
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation1761/range1761/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0061/annotation1761/range1761/1
type: Range
persistent identity: range1761
display id: range1761
version: 1
top level: luxI
direction: direction/forward
start: 1
end: 579
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1760
display id: annotation1760
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation1760/range1760/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0061/annotation1760/range1760/1
type: Range
persistent identity: range1760
display id: range1760
version: 1
top level: luxI
direction: direction/forward
start: 580
end: 611
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213985
display id: annotation2213985
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation2213985/range2213985/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0061/annotation2213985/range2213985/1
type: Range
persistent identity: range2213985
display id: range2213985
version: 1
top level: luxI
direction: direction/forward
start: 619
end: 643
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa C0051
display id: BBa_C0051
version: 1
was generated by: iGEM to SBOL conversion
description: cI repressor from E. coli phage lambda (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999).
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the cI repressor based on cI repressor from bacteriophage lambda modified with an LVA tail for rapid degradation of the protein. cI repressor binds to the cI regulator (BBa_R0051).
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 BBa_C0051 cI repressor is based on the cI repressor from the Elowitz's repressilator. It has been modified to include a rapid degradation LAA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cI lambda
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0051_sequence/1

cI lambda
type: SequenceAnnotation
persistent identity: annotation23334
display id: annotation23334
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
type: Range
persistent identity: range23334
display id: range23334
version: 1
top level: cI lam
direction: direction/forward
start: 4
end: 711
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation23335
display id: annotation23335
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
type: Range
persistent identity: range23335
display id: range23335
version: 1
top level: cI lam
direction: direction/forward
start: 712
end: 744
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213991
display id: annotation2213991
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
type: Range
persistent identity: range2213991
display id: range2213991
version: 1
top level: cI lam
direction: direction/forward
start: 751
end: 775
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa R0051
display id: BBa_R0051
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lambda cI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor <bb_part>BBa_C0051</bb_part>. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (More details needed here! DE, 2/24/03) Incompatible with host expressing cI repressor.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0051
sequence annotation: OR2
sequence annotation: -35
sequence annotation: OR1
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0051_sequence/1

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation7067
display id: annotation7067
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1
type: Range
persistent identity: range7067
display id: range7067
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 49
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2025
display id: annotation2025
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1
type: Range
persistent identity: range2025
display id: range2025
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 17
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2023
display id: annotation2023
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1
type: Range
persistent identity: range2023
display id: range2023
version: 1
top level: cI lam
direction: direction/forward
start: 15
end: 20
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2024
display id: annotation2024
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1
type: Range
persistent identity: range2024
display id: range2024
version: 1
top level: cI lam
direction: direction/forward
start: 25
end: 41
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2022
display id: annotation2022
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1
type: Range
persistent identity: range2022
display id: range2022
version: 1
top level: cI lam
direction: direction/forward
start: 38
end: 43
orientation: inline

luxr
type: ComponentDefinition
persistent identity: BBa C0062
display id: BBa_C0062
version: 1
was generated by: iGEM to SBOL conversion
description: luxR repressor/activator, (no LVA?)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: V. fischeri <genbank>AF170104</genbank>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: 2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in E. coli with codons used at a similar frequency.
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: luxR
sequence annotation: prefix
sequence annotation: BBa_C0062
sequence annotation: T
sequence annotation: A
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0062_sequence/1

luxR
type: SequenceAnnotation
persistent identity: annotation1766
display id: annotation1766
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
type: Range
persistent identity: range1766
display id: range1766
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 750
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation1762
display id: annotation1762
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
type: Range
persistent identity: range1762
display id: range1762
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 2
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation7039
display id: annotation7039
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
type: Range
persistent identity: range7039
display id: range7039
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 756
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation1764
display id: annotation1764
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
type: Range
persistent identity: range1764
display id: range1764
version: 1
top level: luxr
direction: direction/forward
start: 174
end: 174
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation1765
display id: annotation1765
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
type: Range
persistent identity: range1765
display id: range1765
version: 1
top level: luxr
direction: direction/forward
start: 492
end: 492
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213986
display id: annotation2213986
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
type: Range
persistent identity: range2213986
display id: range2213986
version: 1
top level: luxr
direction: direction/forward
start: 757
end: 781
orientation: inline

lux pR
type: ComponentDefinition
persistent identity: BBa R0062
display id: BBa_R0062
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (luxR & HSL regulated -- lux pR)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: V. fischeri
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Promoter activated by LuxR in concert with HSL The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <img src="<bb_file>Image1.gif</bb_file>" width="614" height="362">
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BBa_R0062
sequence annotation: LuxR/HSL
sequence annotation: -35
sequence annotation: -10
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0062_sequence/1

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation7070
display id: annotation7070
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1
type: Range
persistent identity: range7070
display id: range7070
version: 1
top level: lux pR
direction: direction/forward
start: 1
end: 55
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2045
display id: annotation2045
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1
type: Range
persistent identity: range2045
display id: range2045
version: 1
top level: lux pR
direction: direction/forward
start: 1
end: 20
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2046
display id: annotation2046
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1
type: Range
persistent identity: range2046
display id: range2046
version: 1
top level: lux pR
direction: direction/forward
start: 20
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2047
display id: annotation2047
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1
type: Range
persistent identity: range2047
display id: range2047
version: 1
top level: lux pR
direction: direction/forward
start: 42
end: 47
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2048
display id: annotation2048
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1
type: Range
persistent identity: range2048
display id: range2048
version: 1
top level: lux pR
direction: direction/forward
start: 53
end: 53
orientation: inline

lacI
type: ComponentDefinition
persistent identity: BBa C0012
display id: BBa_C0012
version: 1
was generated by: iGEM to SBOL conversion
description: lacI repressor from E. coli (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the LacI protein with an LVA degradation tail and without an RBS. LacI binds to the pLac regulator <bb_part>BBa_R0010</bb_part> and PLlac01 hybrid regulator <bb_part>BBa_R0011</bb_part> and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription. A rapid degredation tail (LVA) has been added to improve the High to Low performance of this part.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] Sequence taken from the repressilator of Elowitz and Leibler (2000). The obtained sequence was compared to the wild-type sequence for LacI obtained through a database search. The sequence had been modified from the wild-type in that wild-type GTG start was changed to an ATG start (note, actual ORF in E.coli has several GTG starts it would seem). The LVA tag has been added for quicker degradation. Incompatible with systems containing LacI, lactose, or IPTG.
star: true
creator: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: BBa_C0012
sequence annotation: lacI-LVA
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0012_sequence/1

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation7031
display id: annotation7031
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1
type: Range
persistent identity: range7031
display id: range7031
version: 1
top level: lacI
direction: direction/forward
start: 1
end: 1128
orientation: inline

lacI-LVA
type: SequenceAnnotation
persistent identity: annotation1723
display id: annotation1723
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1
type: Range
persistent identity: range1723
display id: range1723
version: 1
top level: lacI
direction: direction/forward
start: 1
end: 1128
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1722
display id: annotation1722
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1
type: Range
persistent identity: range1722
display id: range1722
version: 1
top level: lacI
direction: direction/forward
start: 1090
end: 1128
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213988
display id: annotation2213988
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1
type: Range
persistent identity: range2213988
display id: range2213988
version: 1
top level: lacI
direction: direction/forward
start: 1129
end: 1153
orientation: inline

lux pL
type: ComponentDefinition
persistent identity: BBa R0063
display id: BBa_R0063
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (luxR & HSL regulated -- lux pL)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: V. fischeri.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The lux cassette of V. fischeri contains a left and a right promoter. The left promoter gives weak constitutive expression of downstream genes.This expression is down-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription from Pr, repressing transcription from Pl
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below.Includes most of Lux reulatory region, including the LuxR binding site which activates the right promoter. A putative LuxR autorepression binding site is also identified adjacent to the -10 site of the right promoter. This 2nd site has 55% identity with the first site. Putative inverted repeats (of size 18-27 bp) also exist between these two sites (not marked above), which may represent binding sites for other regulatory proteins. <img src="<bb_file>Image01.gif</bb_file>" width="614" height="362"> Unspecified.
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: LuxR/HSL
sequence annotation: BBa_R0063
sequence annotation: -35
sequence annotation: -10
sequence annotation: start
sequence annotation: Putative LuxR/HSL
sequence: https://synbiohub.org/public/igem/BBa_R0063_sequence/1

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2051
display id: annotation2051
version: 1
top level: lux pL
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2051/range2051/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0063/annotation2051/range2051/1
type: Range
persistent identity: range2051
display id: range2051
version: 1
top level: lux pL
direction: direction/forward
start: 1
end: 20
orientation: inline

BBa_R0063
type: SequenceAnnotation
persistent identity: annotation7071
display id: annotation7071
version: 1
top level: lux pL
location: https://synbiohub.org/public/igem/BBa_R0063/annotation7071/range7071/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0063/annotation7071/range7071/1
type: Range
persistent identity: range7071
display id: range7071
version: 1
top level: lux pL
direction: direction/forward
start: 1
end: 151
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2053
display id: annotation2053
version: 1
top level: lux pL
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2053/range2053/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0063/annotation2053/range2053/1
type: Range
persistent identity: range2053
display id: range2053
version: 1
top level: lux pL
direction: direction/forward
start: 89
end: 94
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2052
display id: annotation2052
version: 1
top level: lux pL
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2052/range2052/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0063/annotation2052/range2052/1
type: Range
persistent identity: range2052
display id: range2052
version: 1
top level: lux pL
direction: direction/forward
start: 115
end: 122
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2054
display id: annotation2054
version: 1
top level: lux pL
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2054/range2054/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_R0063/annotation2054/range2054/1
type: Range
persistent identity: range2054
display id: range2054
version: 1
top level: lux pL
direction: direction/forward
start: 128
end: 128
orientation: inline

Putative LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2055
display id: annotation2055
version: 1
top level: lux pL
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2055/range2055/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0063/annotation2055/range2055/1
type: Range
persistent identity: range2055
display id: range2055
version: 1
top level: lux pL
direction: direction/forward
start: 130
end: 149
orientation: inline

ECFP
type: ComponentDefinition
persistent identity: BBa E0022
display id: BBa_E0022
version: 1
was generated by: iGEM to SBOL conversion
description: enhanced cyan fluorescent protein derived from A. victoria GFP
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:25Z
mutable provenance: Modified from <bb_part>BBa_E0021</bb_part>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Cyan fluorescent protein (ECFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_E0022 cyan fluorescent protein is based on BioBrick part BBa_E0021. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence annotation: BBa_E0022
sequence annotation: CFP (LVA)
sequence annotation: A
sequence annotation: SsrA
sequence annotation: 2
sequence: https://synbiohub.org/public/igem/BBa_E0022_sequence/1

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation7040
display id: annotation7040
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation7040/range7040/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_E0022/annotation7040/range7040/1
type: Range
persistent identity: range7040
display id: range7040
version: 1
top level: ECFP
direction: direction/forward
start: 1
end: 762
orientation: inline

CFP (LVA)
type: SequenceAnnotation
persistent identity: annotation2150
display id: annotation2150
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2150/range2150/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E0022/annotation2150/range2150/1
type: Range
persistent identity: range2150
display id: range2150
version: 1
top level: ECFP
direction: direction/forward
start: 1
end: 762
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation2155
display id: annotation2155
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2155/range2155/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_E0022/annotation2155/range2155/1
type: Range
persistent identity: range2155
display id: range2155
version: 1
top level: ECFP
direction: direction/forward
start: 69
end: 69
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation2153
display id: annotation2153
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2153/range2153/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_E0022/annotation2153/range2153/1
type: Range
persistent identity: range2153
display id: range2153
version: 1
top level: ECFP
direction: direction/forward
start: 719
end: 756
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation2154
display id: annotation2154
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2154/range2154/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_E0022/annotation2154/range2154/1
type: Range
persistent identity: range2154
display id: range2154
version: 1
top level: ECFP
direction: direction/forward
start: 757
end: 762
orientation: inline

AraC
type: ComponentDefinition
persistent identity: BBa R0080
display id: BBa_R0080
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (AraC regulated)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: GenBank: J01641 (www.ncbi.nlm.nih.gov)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: AraC operator, truncated to include araO1, araI1, araI2, c-amp1, and c-amp2 sites. This operator should *activate* transcription in the presence of AraC; b/c the operator lacks the araO2 site, there should not be araC-mediated repression.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Sara Neves (Fighting Darwins)
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: c-amp2
sequence annotation: araO1
sequence annotation: c-amp1
sequence annotation: ara1 and ara2
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0080_sequence/1

c-amp2
type: SequenceAnnotation
persistent identity: annotation301456
display id: annotation301456
version: 1
top level: AraC
location: https://synbiohub.org/public/igem/BBa_R0080/annotation301456/range301456/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0080/annotation301456/range301456/1
type: Range
persistent identity: range301456
display id: range301456
version: 1
top level: AraC
direction: direction/forward
start: 4
end: 29
orientation: inline

araO1
type: SequenceAnnotation
persistent identity: annotation301457
display id: annotation301457
version: 1
top level: AraC
location: https://synbiohub.org/public/igem/BBa_R0080/annotation301457/range301457/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0080/annotation301457/range301457/1
type: Range
persistent identity: range301457
display id: range301457
version: 1
top level: AraC
direction: direction/forward
start: 6
end: 44
orientation: inline

c-amp1
type: SequenceAnnotation
persistent identity: annotation301458
display id: annotation301458
version: 1
top level: AraC
location: https://synbiohub.org/public/igem/BBa_R0080/annotation301458/range301458/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0080/annotation301458/range301458/1
type: Range
persistent identity: range301458
display id: range301458
version: 1
top level: AraC
direction: direction/forward
start: 43
end: 72
orientation: inline

ara1 and ara2
type: SequenceAnnotation
persistent identity: annotation301462
display id: annotation301462
version: 1
top level: AraC
location: https://synbiohub.org/public/igem/BBa_R0080/annotation301462/range301462/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0080/annotation301462/range301462/1
type: Range
persistent identity: range301462
display id: range301462
version: 1
top level: AraC
direction: direction/forward
start: 73
end: 101
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation308601
display id: annotation308601
version: 1
top level: AraC
location: https://synbiohub.org/public/igem/BBa_R0080/annotation308601/range308601/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0080/annotation308601/range308601/1
type: Range
persistent identity: range308601
display id: range308601
version: 1
top level: AraC
direction: direction/forward
start: 113
end: 118
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation308602
display id: annotation308602
version: 1
top level: AraC
location: https://synbiohub.org/public/igem/BBa_R0080/annotation308602/range308602/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0080/annotation308602/range308602/1
type: Range
persistent identity: range308602
display id: range308602
version: 1
top level: AraC
direction: direction/forward
start: 136
end: 141
orientation: inline

cI HK022
type: ComponentDefinition
persistent identity: BBa R0050
display id: BBa_R0050
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (HK022 cI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Bacteriophage HK022.
owned by: james
owned by: myers
mutable description: The pR regulatory region is a 98 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two operator sites at which HK022 cI protein can bind cooperatively. The -35 and -10 regions from pR are included along with some flanking sequences. The cutoff for the 5' end of the regulatory region was placed just after the pM -10 region which promotes transcription of a gene upstream (thus the pM -10 region is not included). The cutoff for the 3' end of the regulatory region was placed immediately prior to the RBS.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Derived from <genbank>STHK022N</genbank>. Literature claims that there is no cross-talk between HK022 cI regulatory region, Lambda cI, 434 cI and P22 cI, but this has not been verified experimentally by the synthetic biology group at MIT.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0050
sequence annotation: -35
sequence annotation: OR2
sequence annotation: -10
sequence annotation: OR1
sequence annotation: putative
sequence: https://synbiohub.org/public/igem/BBa_R0050_sequence/1

BBa_R0050
type: SequenceAnnotation
persistent identity: annotation7066
display id: annotation7066
version: 1
top level: cI HK022
location: https://synbiohub.org/public/igem/BBa_R0050/annotation7066/range7066/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0050/annotation7066/range7066/1
type: Range
persistent identity: range7066
display id: range7066
version: 1
top level: cI HK022
direction: direction/forward
start: 1
end: 55
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2012
display id: annotation2012
version: 1
top level: cI HK022
location: https://synbiohub.org/public/igem/BBa_R0050/annotation2012/range2012/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0050/annotation2012/range2012/1
type: Range
persistent identity: range2012
display id: range2012
version: 1
top level: cI HK022
direction: direction/forward
start: 8
end: 13
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2013
display id: annotation2013
version: 1
top level: cI HK022
location: https://synbiohub.org/public/igem/BBa_R0050/annotation2013/range2013/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_R0050/annotation2013/range2013/1
type: Range
persistent identity: range2013
display id: range2013
version: 1
top level: cI HK022
direction: direction/forward
start: 13
end: 27
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2014
display id: annotation2014
version: 1
top level: cI HK022
location: https://synbiohub.org/public/igem/BBa_R0050/annotation2014/range2014/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0050/annotation2014/range2014/1
type: Range
persistent identity: range2014
display id: range2014
version: 1
top level: cI HK022
direction: direction/forward
start: 30
end: 35
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2015
display id: annotation2015
version: 1
top level: cI HK022
location: https://synbiohub.org/public/igem/BBa_R0050/annotation2015/range2015/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_R0050/annotation2015/range2015/1
type: Range
persistent identity: range2015
display id: range2015
version: 1
top level: cI HK022
direction: direction/forward
start: 37
end: 51
orientation: inline

putative
type: SequenceAnnotation
persistent identity: annotation2018
display id: annotation2018
version: 1
top level: cI HK022
location: https://synbiohub.org/public/igem/BBa_R0050/annotation2018/range2018/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_R0050/annotation2018/range2018/1
type: Range
persistent identity: range2018
display id: range2018
version: 1
top level: cI HK022
direction: direction/forward
start: 43
end: 43
orientation: inline

https://synbiohub.org/public/igem/BBa_I3001_sequence/1
type: Sequence
persistent identity: BBa I3001 sequence
display id: BBa_I3001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaagaggagaaatactagatggttcaacagaaagagcgtgaaactttctcgcagaggcttgcgctggcctgtgataaagcgggattacctttgcatggtaggcaggctgatttagctgtcaggcttaaggtcacaccaaaagccattagtaaatggttcaacggggagtcaataccaagaaaagacaagatggaatctctggcttcggtgctgggaactactgctgcatatctgcatggctatgctgatgatgacggtatcacggtaaatcatctatcaagatcaaatgattattatcgtgttgatgtattggatgttcaggcgagcgccgggccaggaaccatggtttccaatgaatttatagaaaagataagagcaattgaatatacgaccgagcaggcaagaattttatttaatggaaggccacaggaaagcgtaaaagtcatcacggttcgcggtgacagcatggagggaaccatcaatccgggagatgagatctttgttgatgtatccataacctgttttgatggcgatggcatttatgtgtttgtatacgggaaaacaatgcacgttaagcgcctgcaaatgcaaaagaacaggcttgccgtcatctctgacaatgccgcttatgatcgatggtacatagaagaaggtgaagaagagcaacttcacattctagccaaagtcctcattaggcagtcaatcgattacaagcgattcggagctgcaaacgacgaaaactacgctttagtagcttaataaccctgatagtgctagtgtagatccctactagagaaagaggagaaatactagatgtgtttaaagcaaatcattggcagcttgaaaaagaaggttcacatgtcaaacaacattcgtatcgaagaagatctgttgggtaccagggaagttccagctgatgcctactatggtgttcacactctgagagcgattgaaaacttctatatcagcaacaacaaaatcagtgatattcctgaatttgttcgcggtatggtaatggttaaaaaagccgcagctatggcaaacaaagagctgcaaaccattcctaaaagtgtagcgaatgccatcattgccgcatgtgatgaagtcctgaacaacggaaaatgcatggatcagttcccggtagacgtctaccagggcggcgcaggtacttccgtaaacatgaacaccaacgaagtgctggccaatatcggtctggaactgatgggtcaccaaaaaggtgaatatcagtacctgaacccgaacgaccatgttaacaaatgtcagtccactaacgacgcctacccgaccggtttccgtatcgcagtttactcttccctgattaagctggtagatgcgattaaccaactgcgtgaaggctttgaacgtaaagctgtcgaatttcaggacatcctgaaaatgggtcgtacccagctgcaagacgcagtaccgatgaccctcggtcaggaatttcgcgctttcagcatcctgctgaaagaagaagtgaaaaacatccaacgtaccgctgaactgctgctggaagttaaccttggtgcaacagcaatcggtactggtctgaacacgccgaaagagtactctccgctggcagtgaaaaaactggctgaagttactggcttcccatgcgtaccggctgaagacctgatcgaagcgacctctgactgcggcgcttatgttatggttcacggcgcgctgaaacgcctggctgtgaagatgtccaaaatctgtaacgacctgcgcttgctctcttcaggcccacgtgccggcctgaacgagatcaacctgccggaactgcaagcgggctcttccatcatgccagctaaagtaaacccggttgttccggaagtggttaaccaggtatgcttcaaagtcatcggtaacgacaccactgttaccatggcagcagaagcaggtcagctgcaattgaa…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0082_sequence/1
type: Sequence
persistent identity: BBa C0082 sequence
display id: BBa_C0082_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgattaaccgtatccgcgtagtcacgctgttggtaatggtgctgggggtattcgcactgttacagcttatttccggcagtctgtttttttcttcccttcaccatagccagaagagctttgtggtttccaatcaattacgggaacagcagggcgagctgacgtcaacctgggatttaatgctgcaaacgcgcattaacctgagtcgttcagcggtacggatgatgatggattcctccaatcaacaaagtaacgccaaagttgaattgctcgatagcgccaggaaaacattggcgcaggcagcgacgcattataaaaaattcaaaagcatggcaccgttacctgaaatggtcgctaccagtcgtaatattgatgaaaaatataaaaactattacacagcgttaactgaactgattgattatctcgattatggcaatactggagcttatttcgctcagccaacccagggaatgcaaaatgcaatgggcgaagcgtttgctcagtacgccctcagcagtgaaaaactgtatcgcgatatcgtcactgacaacgcagatgattaccgatttgcccagtggcaactggcggttatcgcgctggtggtggtattgattctgctggtggcgtggtacggcattcgccgtatgttgcttactccgctggcaaaaattattgctcacattcgcgaaatcgccggtggtaacctggcgaataccctgaccattgacgggcgcagtgaaatgggcgacctggcgcagagcgtttcacatatggcggctggtgttaagcaactggcggatgaccgcacgctgctgatggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcgcctggcgactgagatgatgagcgagcaggatggctatctggcagaatcgatcaataaagatatcgaagagtgcaacgccatcattgagcagtttatcgactacctgcgcaccgggcaggagatgccgatggaaatggcggatcttaatgcagtactcggtgaggtgattgctgccgaaagtggctatgagcgggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaatgcacccgctgtcgatcaaacgcgcggtggcgaatatggtggtcaacgccgcccgttatggcaatggctggatcaaagtcagcagcggaacggagccgaatcgcgcctggttccaggtggaagatgacggtccgggaattgcgccggaacaacgtaagcacctgttccagccgtttgtccgcggcgacagtgcgcgcaccattagcggcacgggattagggctggcaattgtgcagcgtatcgtggataaccataacgggatgctggagcttggcaccagcgagcggggcgggctttccattcgcgcctggctgccagtgccggtaacgcgggcgcagggcacgacaaaagaaggggctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0080_sequence/1
type: Sequence
persistent identity: BBa C0080 sequence
display id: BBa_C0080_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggccaatggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcgccgtcgcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagccaggcgaagctgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcgagtatttaaaaaatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R1075_sequence/1
type: Sequence
persistent identity: BBa R1075 sequence
display id: BBa_R1075_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttaaatttcctcttttcaggccggaataactccctataatgcgccacca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0082_sequence/1
type: Sequence
persistent identity: BBa R0082 sequence
display id: BBa_R0082_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggagaatggact
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0050_sequence/1
type: Sequence
persistent identity: BBa C0050 sequence
display id: BBa_C0050_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggttcaacagaaagagcgtgaaactttctcgcagaggcttgcgctggcctgtgataaagcgggattacctttgcatggtaggcaggctgatttagctgtcaggcttaaggtcacaccaaaagccattagtaaatggttcaacggggagtcaataccaagaaaagacaagatggaatctctggcttcggtgctgggaactactgctgcatatctgcatggctatgctgatgatgacggtatcacggtaaatcatctatcaagatcaaatgattattatcgtgttgatgtattggatgttcaggcgagcgccgggccaggaaccatggtttccaatgaatttatagaaaagataagagcaattgaatatacgaccgagcaggcaagaattttatttaatggaaggccacaggaaagcgtaaaagtcatcacggttcgcggtgacagcatggagggaaccatcaatccgggagatgagatctttgttgatgtatccataacctgttttgatggcgatggcatttatgtgtttgtatacgggaaaacaatgcacgttaagcgcctgcaaatgcaaaagaacaggcttgccgtcatctctgacaatgccgcttatgatcgatggtacatagaagaaggtgaagaagagcaacttcacattctagccaaagtcctcattaggcagtcaatcgattacaagcgattcggagctgcaaacgacgaaaactacgctttagtagcttaataaccctgatagtgctagtgtagatccc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0083_sequence/1
type: Sequence
persistent identity: BBa C0083 sequence
display id: BBa_C0083_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtgtttaaagcaaatcattggcagcttgaaaaagaaggttcacatgtcaaacaacattcgtatcgaagaagatctgttgggtaccagggaagttccagctgatgcctactatggtgttcacactctgagagcgattgaaaacttctatatcagcaacaacaaaatcagtgatattcctgaatttgttcgcggtatggtaatggttaaaaaagccgcagctatggcaaacaaagagctgcaaaccattcctaaaagtgtagcgaatgccatcattgccgcatgtgatgaagtcctgaacaacggaaaatgcatggatcagttcccggtagacgtctaccagggcggcgcaggtacttccgtaaacatgaacaccaacgaagtgctggccaatatcggtctggaactgatgggtcaccaaaaaggtgaatatcagtacctgaacccgaacgaccatgttaacaaatgtcagtccactaacgacgcctacccgaccggtttccgtatcgcagtttactcttccctgattaagctggtagatgcgattaaccaactgcgtgaaggctttgaacgtaaagctgtcgaatttcaggacatcctgaaaatgggtcgtacccagctgcaagacgcagtaccgatgaccctcggtcaggaatttcgcgctttcagcatcctgctgaaagaagaagtgaaaaacatccaacgtaccgctgaactgctgctggaagttaaccttggtgcaacagcaatcggtactggtctgaacacgccgaaagagtactctccgctggcagtgaaaaaactggctgaagttactggcttcccatgcgtaccggctgaagacctgatcgaagcgacctctgactgcggcgcttatgttatggttcacggcgcgctgaaacgcctggctgtgaagatgtccaaaatctgtaacgacctgcgcttgctctcttcaggcccacgtgccggcctgaacgagatcaacctgccggaactgcaagcgggctcttccatcatgccagctaaagtaaacccggttgttccggaagtggttaaccaggtatgcttcaaagtcatcggtaacgacaccactgttaccatggcagcagaagcaggtcagctgcaattgaacgttatggagccggtcattggccaggccatgttcgaatccgttcacattctgaccaacgcttgctacaacctgctggaaaaatgcattaacggcatcactgctaacaaagaagtgtgcgaaggttacgtttacaactctatcggtatcgttacttacctgaacccgttcatcggtcaccacaacggtgacatcgtgggtaaaatctgtgccgaaaccggtaagagtgtacgtgaagtcgttctggaacgcggtctgttgactgaagcggaacttgacgatattttctccgtacagaatctgatgcacccggcttacaaagcaaaacgctatactgatgaaagcgaacaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0061_sequence/1
type: Sequence
persistent identity: BBa C0061 sequence
display id: BBa_C0061_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0051_sequence/1
type: Sequence
persistent identity: BBa C0051 sequence
display id: BBa_C0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0051_sequence/1
type: Sequence
persistent identity: BBa R0051 sequence
display id: BBa_R0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taacaccgtgcgtgttgactattttacctctggcggtgataatggttgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0062_sequence/1
type: Sequence
persistent identity: BBa C0062 sequence
display id: BBa_C0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0062_sequence/1
type: Sequence
persistent identity: BBa R0062 sequence
display id: BBa_R0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0012_sequence/1
type: Sequence
persistent identity: BBa C0012 sequence
display id: BBa_C0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0063_sequence/1
type: Sequence
persistent identity: BBa R0063 sequence
display id: BBa_R0063_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtacgatcctacaggtgcttatgttaagtaattgtattcccagcgatacaatagtgtgacaaaaatccaatttattagaatcaaatgtcaatccattaccgttttaatgatatataacacgcaaaacttgcgacaaacaataggtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0022_sequence/1
type: Sequence
persistent identity: BBa E0022 sequence
display id: BBa_E0022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0080_sequence/1
type: Sequence
persistent identity: BBa R0080 sequence
display id: BBa_R0080_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0050_sequence/1
type: Sequence
persistent identity: BBa R0050 sequence
display id: BBa_R0050_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttaggtattgactgtactatcagttccgtcataatatgaaccataagttcaccac
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z