LOCUS BBa_I3002 13449 bp DNA linear 22-Dec-2024 DEFINITION E. colibrator design 3, Polkadorks (I3410.I3200.E0422.I3510.I3401.I 3411.I0460.I3100.I3400.I3300.I340 ACCESSION BBa_I3002 VERSION BBa_I3002.1 FEATURES Location/Qualifiers promoter 38..43 /label=-10 promoter 15..20 /label=-35 misc_feature 25..41 /label=OR1 misc_feature 1..17 /label=OR2 misc_feature 1..49 /label=BBa_R0051 promoter 1..49 /label=BBa_R0051 misc_feature 62..65 /label=conserved RBS 58..69 /label=BBa_B0034 misc_feature 814..819 /label=2 CDS 76..819 /label=cI HK022 misc_feature 781..813 /label=SsrA misc_feature 76..83 /label=OR3 partial misc_feature 76..819 /label=BBa_C0050 misc_feature 820..844 /label=Help:Barcodes CDS 76..819 /label=BBa_C0050 misc_feature 857..860 /label=conserved RBS 853..864 /label=BBa_B0034 CDS 871..2349 /label=AspA misc_feature 871..871 /label=G misc_feature 1458..1458 /label=C misc_feature 1494..1494 /label=G misc_feature 1527..1527 /label=C misc_feature 1869..1869 /label=G misc_feature 1995..1995 /label=G misc_feature 2350..2382 /label=LVA misc_feature 2389..2413 /label=Help:Barcodes CDS 871..2388 /label=BBa_C0083 misc_feature 2426..2429 /label=conserved RBS 2422..2433 /label=BBa_B0034 CDS 2440..3201 /label=CFP (LVA) misc_feature 3158..3195 /label=SsrA misc_feature 3196..3201 /label=2 misc_feature 2508..2508 /label=A misc_feature 2440..3201 /label=BBa_E0022 misc_feature 2440..3201 /label=BBa_E0022 stem_loop 3221..3264 /label=stem_loop misc_feature 3210..3289 /label=BBa_B0010 terminator 3210..3289 /label=BBa_B0010 stem_loop 3305..3324 /label=T7 TE misc_feature 3331..3331 /label=stop polyA_site 3325..3338 /label=polya misc_feature 3298..3338 /label=BBa_B0012 terminator 3298..3338 /label=BBa_B0012 misc_feature 3347..3366 /label=LuxR/HSL promoter 3461..3468 /label=-10 promoter 3435..3440 /label=-35 misc_feature 3474..3474 /label=start misc_feature 3476..3495 /label=Putative LuxR/HSL misc_feature 3347..3497 /label=BBa_R0063 promoter 3347..3497 /label=BBa_R0063 misc_feature 3510..3513 /label=conserved RBS 3506..3517 /label=BBa_B0034 misc_feature 3524..3525 /label=prefix misc_feature 3697..3697 /label=T misc_feature 4015..4015 /label=A CDS 3524..4273 /label=luxR misc_feature 3524..4279 /label=BBa_C0062 misc_feature 4280..4304 /label=Help:Barcodes CDS 3524..4279 /label=BBa_C0062 stem_loop 4324..4367 /label=stem_loop misc_feature 4313..4392 /label=BBa_B0010 terminator 4313..4392 /label=BBa_B0010 stem_loop 4408..4427 /label=T7 TE misc_feature 4434..4434 /label=stop polyA_site 4428..4441 /label=polya misc_feature 4401..4441 /label=BBa_B0012 terminator 4401..4441 /label=BBa_B0012 misc_feature 4450..4469 /label=LuxR/HSL promoter 4469..4474 /label=-35 promoter 4491..4496 /label=-10 misc_feature 4502..4502 /label=start misc_feature 4450..4504 /label=BBa_R0062 promoter 4450..4504 /label=BBa_R0062 misc_feature 4517..4520 /label=conserved RBS 4513..4524 /label=BBa_B0034 CDS 4534..5241 /label=cI lambda misc_feature 5242..5274 /label=LVA misc_feature 5281..5305 /label=Help:Barcodes CDS 4531..5280 /label=BBa_C0051 stem_loop 5325..5368 /label=stem_loop misc_feature 5314..5393 /label=BBa_B0010 terminator 5314..5393 /label=BBa_B0010 stem_loop 5409..5428 /label=T7 TE misc_feature 5435..5435 /label=stop polyA_site 5429..5442 /label=polya misc_feature 5402..5442 /label=BBa_B0012 terminator 5402..5442 /label=BBa_B0012 promoter 5458..5463 /label=-35 RBS 5463..5477 /label=OR2 promoter 5480..5485 /label=-10 RBS 5487..5501 /label=OR1 misc_feature 5493..5493 /label=putative misc_feature 5451..5505 /label=BBa_R0050 promoter 5451..5505 /label=BBa_R0050 misc_feature 5518..5521 /label=conserved RBS 5514..5525 /label=BBa_B0034 CDS 5535..6242 /label=cI lambda misc_feature 6243..6275 /label=LVA misc_feature 6282..6306 /label=Help:Barcodes CDS 5532..6281 /label=BBa_C0051 misc_feature 6319..6322 /label=conserved RBS 6315..6326 /label=BBa_B0034 misc_feature 6333..6335 /label=start misc_feature 7116..7121 /label=2 misc_feature 7083..7115 /label=LVA CDS 6333..7082 /label=aiiA misc_feature 6333..7121 /label=BBa_C0060 misc_feature 7122..7146 /label=Help:Barcodes CDS 6333..7121 /label=BBa_C0060 stem_loop 7166..7209 /label=stem_loop misc_feature 7155..7234 /label=BBa_B0010 terminator 7155..7234 /label=BBa_B0010 stem_loop 7250..7269 /label=T7 TE misc_feature 7276..7276 /label=stop polyA_site 7270..7283 /label=polya misc_feature 7243..7283 /label=BBa_B0012 terminator 7243..7283 /label=BBa_B0012 promoter 7326..7331 /label=-10 promoter 7306..7311 /label=-35 promoter 7292..7340 /label=BBa_R1075 misc_feature 7353..7356 /label=conserved RBS 7349..7360 /label=BBa_B0034 misc_feature 8456..8494 /label=LVA CDS 7367..8494 /label=lacI-LVA misc_feature 7367..8494 /label=BBa_C0012 misc_feature 8495..8519 /label=Help:Barcodes CDS 7367..8494 /label=BBa_C0012 stem_loop 8539..8582 /label=stem_loop misc_feature 8528..8607 /label=BBa_B0010 terminator 8528..8607 /label=BBa_B0010 stem_loop 8623..8642 /label=T7 TE misc_feature 8649..8649 /label=stop polyA_site 8643..8656 /label=polya misc_feature 8616..8656 /label=BBa_B0012 terminator 8616..8656 /label=BBa_B0012 CDS 8665..8752 /label=end of LacI coding region (inactive) misc_feature 8665..8864 /label=BBa_R0010 misc_feature 8753..8790 /label=CAP binding site promoter 8801..8806 /label=-35 promoter 8825..8830 /label=-10 misc_feature 8830..8864 /label=LacI binding site misc_feature 8837..8837 /label=start promoter 8665..8864 /label=BBa_R0010 misc_feature 8877..8880 /label=conserved RBS 8873..8884 /label=BBa_B0034 misc_feature 9629..9634 /label=2 CDS 8891..9634 /label=cI HK022 misc_feature 9596..9628 /label=SsrA misc_feature 8891..8898 /label=OR3 partial misc_feature 8891..9634 /label=BBa_C0050 misc_feature 9635..9659 /label=Help:Barcodes CDS 8891..9634 /label=BBa_C0050 stem_loop 9679..9722 /label=stem_loop misc_feature 9668..9747 /label=BBa_B0010 terminator 9668..9747 /label=BBa_B0010 stem_loop 9763..9782 /label=T7 TE misc_feature 9789..9789 /label=stop polyA_site 9783..9796 /label=polya misc_feature 9756..9796 /label=BBa_B0012 terminator 9756..9796 /label=BBa_B0012 promoter 9839..9844 /label=-10 promoter 9819..9824 /label=-35 promoter 9805..9853 /label=BBa_R1075 misc_feature 9866..9869 /label=conserved RBS 9862..9873 /label=BBa_B0034 misc_feature 10302..10303 /label=A misc_feature 11332..11364 /label=LVA CDS 9880..10644 /label=Tar (front) CDS 10651..11331 /label=Envz (back) misc_feature 11371..11395 /label=Help:Barcodes CDS 9880..11370 /label=BBa_C0082 stem_loop 11415..11458 /label=stem_loop misc_feature 11404..11483 /label=BBa_B0010 terminator 11404..11483 /label=BBa_B0010 stem_loop 11499..11518 /label=T7 TE misc_feature 11525..11525 /label=stop polyA_site 11519..11532 /label=polya misc_feature 11492..11532 /label=BBa_B0012 terminator 11492..11532 /label=BBa_B0012 misc_feature 11553..11570 /label=C1 OmpR misc_feature 11574..11591 /label=C2 OmpR misc_feature 11594..11611 /label=C3 OmpR promoter 11615..11620 /label=-35 promoter 11638..11643 /label=-10 promoter 11541..11648 /label=BBa_R0082 misc_feature 11661..11664 /label=conserved RBS 11657..11668 /label=BBa_B0034 misc_feature 12413..12418 /label=2 CDS 11675..12418 /label=cI HK022 misc_feature 12380..12412 /label=SsrA misc_feature 11675..11682 /label=OR3 partial misc_feature 11675..12418 /label=BBa_C0050 misc_feature 12419..12443 /label=Help:Barcodes CDS 11675..12418 /label=BBa_C0050 stem_loop 12463..12506 /label=stem_loop misc_feature 12452..12531 /label=BBa_B0010 terminator 12452..12531 /label=BBa_B0010 stem_loop 12547..12566 /label=T7 TE misc_feature 12573..12573 /label=stop polyA_site 12567..12580 /label=polya misc_feature 12540..12580 /label=BBa_B0012 terminator 12540..12580 /label=BBa_B0012 misc_feature 12589..12608 /label=LuxR/HSL promoter 12608..12613 /label=-35 promoter 12630..12635 /label=-10 misc_feature 12641..12641 /label=start misc_feature 12589..12643 /label=BBa_R0062 promoter 12589..12643 /label=BBa_R0062 misc_feature 12656..12659 /label=conserved RBS 12652..12663 /label=BBa_B0034 misc_feature 13249..13280 /label=LVA CDS 12670..13248 /label=luxI misc_feature 12670..13287 /label=BBa_C0061 misc_feature 13288..13312 /label=Help:Barcodes CDS 12670..13287 /label=BBa_C0061 stem_loop 13332..13375 /label=stem_loop misc_feature 13321..13400 /label=BBa_B0010 terminator 13321..13400 /label=BBa_B0010 stem_loop 13416..13435 /label=T7 TE misc_feature 13442..13442 /label=stop polyA_site 13436..13449 /label=polya misc_feature 13409..13449 /label=BBa_B0012 terminator 13409..13449 /label=BBa_B0012 ORIGIN 1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagaaa 61 gaggagaaat actagatggt tcaacagaaa gagcgtgaaa ctttctcgca gaggcttgcg 121 ctggcctgtg ataaagcggg attacctttg catggtaggc aggctgattt agctgtcagg 181 cttaaggtca caccaaaagc cattagtaaa tggttcaacg gggagtcaat accaagaaaa 241 gacaagatgg aatctctggc ttcggtgctg ggaactactg ctgcatatct gcatggctat 301 gctgatgatg acggtatcac ggtaaatcat ctatcaagat caaatgatta ttatcgtgtt 361 gatgtattgg atgttcaggc gagcgccggg ccaggaacca tggtttccaa tgaatttata 421 gaaaagataa gagcaattga atatacgacc gagcaggcaa gaattttatt taatggaagg 481 ccacaggaaa gcgtaaaagt catcacggtt cgcggtgaca gcatggaggg aaccatcaat 541 ccgggagatg agatctttgt tgatgtatcc ataacctgtt ttgatggcga tggcatttat 601 gtgtttgtat acgggaaaac aatgcacgtt aagcgcctgc aaatgcaaaa gaacaggctt 661 gccgtcatct ctgacaatgc cgcttatgat cgatggtaca tagaagaagg tgaagaagag 721 caacttcaca ttctagccaa agtcctcatt aggcagtcaa tcgattacaa gcgattcgga 781 gctgcaaacg acgaaaacta cgctttagta gcttaataac cctgatagtg ctagtgtaga 841 tccctactag agaaagagga gaaatactag atgtgtttaa agcaaatcat tggcagcttg 901 aaaaagaagg ttcacatgtc aaacaacatt cgtatcgaag aagatctgtt gggtaccagg 961 gaagttccag ctgatgccta ctatggtgtt cacactctga gagcgattga aaacttctat 1021 atcagcaaca acaaaatcag tgatattcct gaatttgttc gcggtatggt aatggttaaa 1081 aaagccgcag ctatggcaaa caaagagctg caaaccattc ctaaaagtgt agcgaatgcc 1141 atcattgccg catgtgatga agtcctgaac aacggaaaat gcatggatca gttcccggta 1201 gacgtctacc agggcggcgc aggtacttcc gtaaacatga acaccaacga agtgctggcc 1261 aatatcggtc tggaactgat gggtcaccaa aaaggtgaat atcagtacct gaacccgaac 1321 gaccatgtta acaaatgtca gtccactaac gacgcctacc cgaccggttt ccgtatcgca 1381 gtttactctt ccctgattaa gctggtagat gcgattaacc aactgcgtga aggctttgaa 1441 cgtaaagctg tcgaatttca ggacatcctg aaaatgggtc gtacccagct gcaagacgca 1501 gtaccgatga ccctcggtca ggaatttcgc gctttcagca tcctgctgaa agaagaagtg 1561 aaaaacatcc aacgtaccgc tgaactgctg ctggaagtta accttggtgc aacagcaatc 1621 ggtactggtc tgaacacgcc gaaagagtac tctccgctgg cagtgaaaaa actggctgaa 1681 gttactggct tcccatgcgt accggctgaa gacctgatcg aagcgacctc tgactgcggc 1741 gcttatgtta tggttcacgg cgcgctgaaa cgcctggctg tgaagatgtc caaaatctgt 1801 aacgacctgc gcttgctctc ttcaggccca cgtgccggcc tgaacgagat caacctgccg 1861 gaactgcaag cgggctcttc catcatgcca gctaaagtaa acccggttgt tccggaagtg 1921 gttaaccagg tatgcttcaa agtcatcggt aacgacacca ctgttaccat ggcagcagaa 1981 gcaggtcagc tgcaattgaa cgttatggag ccggtcattg gccaggccat gttcgaatcc 2041 gttcacattc tgaccaacgc ttgctacaac ctgctggaaa aatgcattaa cggcatcact 2101 gctaacaaag aagtgtgcga aggttacgtt tacaactcta tcggtatcgt tacttacctg 2161 aacccgttca tcggtcacca caacggtgac atcgtgggta aaatctgtgc cgaaaccggt 2221 aagagtgtac gtgaagtcgt tctggaacgc ggtctgttga ctgaagcgga acttgacgat 2281 attttctccg tacagaatct gatgcacccg gcttacaaag caaaacgcta tactgatgaa 2341 agcgaacagg ctgcaaacga cgaaaactac gctttagtag cttaataact ctgatagtgc 2401 tagtgtagat ctctactaga gaaagaggag aaatactaga tggtgagcaa gggcgaggag 2461 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtgaa cggccacaag 2521 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc 2581 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctgg 2641 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc 2701 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac 2761 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag 2821 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacatc 2881 agccacaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag 2941 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc 3001 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc 3061 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc 3121 gccgggatca ctctcggcat ggacgagctg tacaagaggc ctgctgcaaa cgacgaaaac 3181 tacgctttag tagcttaata atactagagc caggcatcaa ataaaacgaa aggctcagtc 3241 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 3301 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtacgatcct 3361 acaggtgctt atgttaagta attgtattcc cagcgataca atagtgtgac aaaaatccaa 3421 tttattagaa tcaaatgtca atccattacc gttttaatga tatataacac gcaaaacttg 3481 cgacaaacaa taggtaatac tagagaaaga ggagaaatac tagatgaaaa acataaatgc 3541 cgacgacaca tacagaataa ttaataaaat taaagcttgt agaagcaata atgatattaa 3601 tcaatgctta tctgatatga ctaaaatggt acattgtgaa tattatttac tcgcgatcat 3661 ttatcctcat tctatggtta aatctgatat ttcaatccta gataattacc ctaaaaaatg 3721 gaggcaatat tatgatgacg ctaatttaat aaaatatgat cctatagtag attattctaa 3781 ctccaatcat tcaccaatta attggaatat atttgaaaac aatgctgtaa ataaaaaatc 3841 tccaaatgta attaaagaag cgaaaacatc aggtcttatc actgggttta gtttccctat 3901 tcatacggct aacaatggct tcggaatgct tagttttgca cattcagaaa aagacaacta 3961 tatagatagt ttatttttac atgcgtgtat gaacatacca ttaattgttc cttctctagt 4021 tgataattat cgaaaaataa atatagcaaa taataaatca aacaacgatt taaccaaaag 4081 agaaaaagaa tgtttagcgt gggcatgcga aggaaaaagc tcttgggata tttcaaaaat 4141 attaggttgc agtgagcgta ctgtcacttt ccatttaacc aatgcgcaaa tgaaactcaa 4201 tacaacaaac cgctgccaaa gtatttctaa agcaatttta acaggagcaa ttgattgccc 4261 atactttaaa aattaataac actgatagtg ctagtgtaga tcactactag agccaggcat 4321 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 4381 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 4441 atactagaga cctgtaggat cgtacaggtt tacgcaagaa aatggtttgt tatagtcgaa 4501 taaatactag agaaagagga gaaatactag atgagcacaa aaaagaaacc attaacacaa 4561 gagcagcttg aggacgcacg tcgccttaaa gcaatttatg aaaaaaagaa aaatgaactt 4621 ggcttatccc aggaatctgt cgcagacaag atggggatgg ggcagtcagg cgttggtgct 4681 ttatttaatg gcatcaatgc attaaatgct tataacgccg cattgcttgc aaaaattctc 4741 aaagttagcg ttgaagaatt tagcccttca atcgccagag aaatctacga gatgtatgaa 4801 gcggttagta tgcagccgtc acttagaagt gagtatgagt accctgtttt ttctcatgtt 4861 caggcaggga tgttctcacc tgagcttaga acctttacca aaggtgatgc ggagagatgg 4921 gtaagcacaa ccaaaaaagc cagtgattct gcattctggc ttgaggttga aggtaattcc 4981 atgaccgcac caacaggctc caagccaagc tttcctgacg gaatgttaat tctcgttgac 5041 cctgagcagg ctgttgagcc aggtgatttc tgcatagcca gacttggggg tgatgagttt 5101 accttcaaga aactgatcag ggatagcggt caggtgtttt tacaaccact aaacccacag 5161 tacccaatga tcccatgcaa tgagagttgt tccgttgtgg ggaaagttat cgctagtcag 5221 tggcctgaag agacgtttgg cgctgcaaac gacgaaaact acgctttagt agcttaataa 5281 cgctgatagt gctagtgtag atcgctacta gagccaggca tcaaataaaa cgaaaggctc 5341 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga 5401 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag ttaggtattg 5461 actgtactat cagttccgtc ataatatgaa ccataagttc accactacta gagaaagagg 5521 agaaatacta gatgagcaca aaaaagaaac cattaacaca agagcagctt gaggacgcac 5581 gtcgccttaa agcaatttat gaaaaaaaga aaaatgaact tggcttatcc caggaatctg 5641 tcgcagacaa gatggggatg gggcagtcag gcgttggtgc tttatttaat ggcatcaatg 5701 cattaaatgc ttataacgcc gcattgcttg caaaaattct caaagttagc gttgaagaat 5761 ttagcccttc aatcgccaga gaaatctacg agatgtatga agcggttagt atgcagccgt 5821 cacttagaag tgagtatgag taccctgttt tttctcatgt tcaggcaggg atgttctcac 5881 ctgagcttag aacctttacc aaaggtgatg cggagagatg ggtaagcaca accaaaaaag 5941 ccagtgattc tgcattctgg cttgaggttg aaggtaattc catgaccgca ccaacaggct 6001 ccaagccaag ctttcctgac ggaatgttaa ttctcgttga ccctgagcag gctgttgagc 6061 caggtgattt ctgcatagcc agacttgggg gtgatgagtt taccttcaag aaactgatca 6121 gggatagcgg tcaggtgttt ttacaaccac taaacccaca gtacccaatg atcccatgca 6181 atgagagttg ttccgttgtg gggaaagtta tcgctagtca gtggcctgaa gagacgtttg 6241 gcgctgcaaa cgacgaaaac tacgctttag tagcttaata acgctgatag tgctagtgta 6301 gatcgctact agagaaagag gagaaatact agatgacagt aaagaagctt tatttcgtcc 6361 cagcaggtcg ttgtatgttg gatcattcgt ctgttaatag tacattaaca ccaggagaat 6421 tattagactt accggtttgg tgttatcttt tggagactga agaaggacct attttagtag 6481 atacaggtat gccagaaagt gcagttaata atgaaggtct ttttaacggt acatttgtcg 6541 aagggcaggt tttaccgaaa atgactgaag aagatagaat cgtgaatatt ttaaaacggg 6601 ttggttatga gccggaagac cttctttata ttattagttc tcacttgcat tttgatcatg 6661 caggaggaaa tggcgctttt ataaatacac caatcattgt acagcgtgct gaatatgagg 6721 cggcgcagca tagcgaagaa tatttgaaag aatgtatatt gccgaattta aactacaaaa 6781 tcattgaagg tgattatgaa gtcgtaccag gagttcaatt attgcataca ccaggccata 6841 ctccagggca tcaatcgcta ttaattgaga cagaaaaatc cggtcctgta ttattaacga 6901 ttgatgcatc gtatacgaaa gagaattttg aaaatgaagt gccatttgcg ggatttgatt 6961 cagaattagc tttatcttca attaaacgtt taaaagaagt ggtgatgaaa gagaagccga 7021 ttgttttctt tggacatgat atagagcagg aaaggggatg taaagtgttc cctgaatata 7081 tagctgcaaa cgacgaaaac tacgctttag tagcttaata acgctgatag tgctagtgta 7141 gatcgctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt 7201 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc 7261 gggtgggcct ttctgcgttt atatactaga gttaaatttc ctcttttcag gccggaataa 7321 ctccctataa tgcgccacca tactagagaa agaggagaaa tactagatgg tgaatgtgaa 7381 accagtaacg ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg 7441 cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat 7501 ggcggagctg aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt 7561 gctgattggc gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc 7621 gattaaatct cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag 7681 cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct 7741 gatcattaac tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa 7801 tgttccggcg ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc 7861 ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat 7921 cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca 7981 taaatatctc actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc 8041 catgtccggt tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat 8101 gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct 8161 gcgcgttggt gcggatatct cggtagtggg atacgacgat accgaagaca gctcatgtta 8221 tatcccgccg ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga 8281 ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc 8341 actggtgaaa agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt 8401 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcaggctgc 8461 aaacgacgaa aactacgctt tagtagctta ataactctga tagtgctagt gtagatctct 8521 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt 8581 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg 8641 cctttctgcg tttatatact agagcaatac gcaaaccgcc tctccccgcg cgttggccga 8701 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg 8761 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg 8821 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacatactag agaaagagga 8881 gaaatactag atggttcaac agaaagagcg tgaaactttc tcgcagaggc ttgcgctggc 8941 ctgtgataaa gcgggattac ctttgcatgg taggcaggct gatttagctg tcaggcttaa 9001 ggtcacacca aaagccatta gtaaatggtt caacggggag tcaataccaa gaaaagacaa 9061 gatggaatct ctggcttcgg tgctgggaac tactgctgca tatctgcatg gctatgctga 9121 tgatgacggt atcacggtaa atcatctatc aagatcaaat gattattatc gtgttgatgt 9181 attggatgtt caggcgagcg ccgggccagg aaccatggtt tccaatgaat ttatagaaaa 9241 gataagagca attgaatata cgaccgagca ggcaagaatt ttatttaatg gaaggccaca 9301 ggaaagcgta aaagtcatca cggttcgcgg tgacagcatg gagggaacca tcaatccggg 9361 agatgagatc tttgttgatg tatccataac ctgttttgat ggcgatggca tttatgtgtt 9421 tgtatacggg aaaacaatgc acgttaagcg cctgcaaatg caaaagaaca ggcttgccgt 9481 catctctgac aatgccgctt atgatcgatg gtacatagaa gaaggtgaag aagagcaact 9541 tcacattcta gccaaagtcc tcattaggca gtcaatcgat tacaagcgat tcggagctgc 9601 aaacgacgaa aactacgctt tagtagctta ataaccctga tagtgctagt gtagatccct 9661 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt 9721 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg 9781 cctttctgcg tttatatact agagttaaat ttcctctttt caggccggaa taactcccta 9841 taatgcgcca ccatactaga gaaagaggag aaatactaga tgattaaccg tatccgcgta 9901 gtcacgctgt tggtaatggt gctgggggta ttcgcactgt tacagcttat ttccggcagt 9961 ctgttttttt cttcccttca ccatagccag aagagctttg tggtttccaa tcaattacgg 10021 gaacagcagg gcgagctgac gtcaacctgg gatttaatgc tgcaaacgcg cattaacctg 10081 agtcgttcag cggtacggat gatgatggat tcctccaatc aacaaagtaa cgccaaagtt 10141 gaattgctcg atagcgccag gaaaacattg gcgcaggcag cgacgcatta taaaaaattc 10201 aaaagcatgg caccgttacc tgaaatggtc gctaccagtc gtaatattga tgaaaaatat 10261 aaaaactatt acacagcgtt aactgaactg attgattatc tcgattatgg caatactgga 10321 gcttatttcg ctcagccaac ccagggaatg caaaatgcaa tgggcgaagc gtttgctcag 10381 tacgccctca gcagtgaaaa actgtatcgc gatatcgtca ctgacaacgc agatgattac 10441 cgatttgccc agtggcaact ggcggttatc gcgctggtgg tggtattgat tctgctggtg 10501 gcgtggtacg gcattcgccg tatgttgctt actccgctgg caaaaattat tgctcacatt 10561 cgcgaaatcg ccggtggtaa cctggcgaat accctgacca ttgacgggcg cagtgaaatg 10621 ggcgacctgg cgcagagcgt ttcacatatg gcggctggtg ttaagcaact ggcggatgac 10681 cgcacgctgc tgatggcggg ggtaagtcac gacttgcgca cgccgctgac gcgtattcgc 10741 ctggcgactg agatgatgag cgagcaggat ggctatctgg cagaatcgat caataaagat 10801 atcgaagagt gcaacgccat cattgagcag tttatcgact acctgcgcac cgggcaggag 10861 atgccgatgg aaatggcgga tcttaatgca gtactcggtg aggtgattgc tgccgaaagt 10921 ggctatgagc gggaaattga aaccgcgctt taccccggca gcattgaagt gaaaatgcac 10981 ccgctgtcga tcaaacgcgc ggtggcgaat atggtggtca acgccgcccg ttatggcaat 11041 ggctggatca aagtcagcag cggaacggag ccgaatcgcg cctggttcca ggtggaagat 11101 gacggtccgg gaattgcgcc ggaacaacgt aagcacctgt tccagccgtt tgtccgcggc 11161 gacagtgcgc gcaccattag cggcacggga ttagggctgg caattgtgca gcgtatcgtg 11221 gataaccata acgggatgct ggagcttggc accagcgagc ggggcgggct ttccattcgc 11281 gcctggctgc cagtgccggt aacgcgggcg cagggcacga caaaagaagg ggctgcaaac 11341 gacgaaaact acgctttagt agcttaataa cgctgatagt gctagtgtag atcgctacta 11401 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct 11461 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt 11521 tctgcgttta tatactagag tcccttgcat ttacattttg aaacatctat agcgataaat 11581 gaaacatctt aaaagtttta gtatcatatt cgtgttggat tattctgcat ttttggggag 11641 aatggactta ctagagaaag aggagaaata ctagatggtt caacagaaag agcgtgaaac 11701 tttctcgcag aggcttgcgc tggcctgtga taaagcggga ttacctttgc atggtaggca 11761 ggctgattta gctgtcaggc ttaaggtcac accaaaagcc attagtaaat ggttcaacgg 11821 ggagtcaata ccaagaaaag acaagatgga atctctggct tcggtgctgg gaactactgc 11881 tgcatatctg catggctatg ctgatgatga cggtatcacg gtaaatcatc tatcaagatc 11941 aaatgattat tatcgtgttg atgtattgga tgttcaggcg agcgccgggc caggaaccat 12001 ggtttccaat gaatttatag aaaagataag agcaattgaa tatacgaccg agcaggcaag 12061 aattttattt aatggaaggc cacaggaaag cgtaaaagtc atcacggttc gcggtgacag 12121 catggaggga accatcaatc cgggagatga gatctttgtt gatgtatcca taacctgttt 12181 tgatggcgat ggcatttatg tgtttgtata cgggaaaaca atgcacgtta agcgcctgca 12241 aatgcaaaag aacaggcttg ccgtcatctc tgacaatgcc gcttatgatc gatggtacat 12301 agaagaaggt gaagaagagc aacttcacat tctagccaaa gtcctcatta ggcagtcaat 12361 cgattacaag cgattcggag ctgcaaacga cgaaaactac gctttagtag cttaataacc 12421 ctgatagtgc tagtgtagat ccctactaga gccaggcatc aaataaaacg aaaggctcag 12481 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt 12541 cacactggct caccttcggg tgggcctttc tgcgtttata tactagagac ctgtaggatc 12601 gtacaggttt acgcaagaaa atggtttgtt atagtcgaat aaatactaga gaaagaggag 12661 aaatactaga tgactataat gataaaaaaa tcggattttt tggcaattcc atcggaggag 12721 tataaaggta ttctaagtct tcgttatcaa gtgtttaagc aaagacttga gtgggactta 12781 gttgtagaaa ataaccttga atcagatgag tatgataact caaatgcaga atatatttat 12841 gcttgtgatg atactgaaaa tgtaagtgga tgctggcgtt tattacctac aacaggtgat 12901 tatatgctga aaagtgtttt tcctgaattg cttggtcaac agagtgctcc caaagatcct 12961 aatatagtcg aattaagtcg ttttgctgta ggtaaaaata gctcaaagat aaataactct 13021 gctagtgaaa ttacaatgaa actatttgaa gctatatata aacacgctgt tagtcaaggt 13081 attacagaat atgtaacagt aacatcaaca gcaatagagc gatttttaaa gcgtattaaa 13141 gttccttgtc atcgtattgg agacaaagaa attcatgtat taggtgatac taaatcggtt 13201 gtattgtcta tgcctattaa tgaacagttt aaaaaagcag tcttaaatgc tgcaaacgac 13261 gaaaactacg ctttagtagc ttaataactc tgatagtgct agtgtagatc tctactagag 13321 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 13381 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 13441 gcgtttata //