LOCUS       BBa_I3110               2498 bp    DNA     linear       02-May-2024
DEFINITION  Test: Lac trigger (I3100.E0422)
ACCESSION   BBa_I3110
VERSION     BBa_I3110.1  
FEATURES             Location/Qualifiers
     promoter        35..40
                     /label=-10
     promoter        15..20
                     /label=-35
     promoter        1..49
                     /label=BBa_R1075
     misc_feature    62..65
                     /label=conserved
     RBS             58..69
                     /label=BBa_B0034
     misc_feature    1165..1203
                     /label=LVA
     CDS             76..1203
                     /label=lacI-LVA
     misc_feature    76..1203
                     /label=BBa_C0012
     misc_feature    1204..1228
                     /label=Help:Barcodes
     CDS             76..1203
                     /label=BBa_C0012
     stem_loop       1248..1291
                     /label=stem_loop
     misc_feature    1237..1316
                     /label=BBa_B0010
     terminator      1237..1316
                     /label=BBa_B0010
     stem_loop       1332..1351
                     /label=T7 TE
     misc_feature    1358..1358
                     /label=stop
     polyA_site      1352..1365
                     /label=polya
     misc_feature    1325..1365
                     /label=BBa_B0012
     terminator      1325..1365
                     /label=BBa_B0012
     CDS             1374..1461
                     /label=end of LacI coding region (inactive)
     misc_feature    1374..1573
                     /label=BBa_R0010
     misc_feature    1462..1499
                     /label=CAP binding site
     promoter        1510..1515
                     /label=-35
     promoter        1534..1539
                     /label=-10
     misc_feature    1539..1573
                     /label=LacI binding site
     misc_feature    1546..1546
                     /label=start
     promoter        1374..1573
                     /label=BBa_R0010
     misc_feature    1586..1589
                     /label=conserved
     RBS             1582..1593
                     /label=BBa_B0034
     CDS             1600..2361
                     /label=CFP (LVA)
     misc_feature    2318..2355
                     /label=SsrA
     misc_feature    2356..2361
                     /label=2
     misc_feature    1668..1668
                     /label=A
     misc_feature    1600..2361
                     /label=BBa_E0022
     misc_feature    1600..2361
                     /label=BBa_E0022
     stem_loop       2381..2424
                     /label=stem_loop
     misc_feature    2370..2449
                     /label=BBa_B0010
     terminator      2370..2449
                     /label=BBa_B0010
     stem_loop       2465..2484
                     /label=T7 TE
     misc_feature    2491..2491
                     /label=stop
     polyA_site      2485..2498
                     /label=polya
     misc_feature    2458..2498
                     /label=BBa_B0012
     terminator      2458..2498
                     /label=BBa_B0012
ORIGIN
        1 ttaaatttcc tcttttcagg ccggaataac tccctataat gcgccaccat actagagaaa
       61 gaggagaaat actagatggt gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat
      121 gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg
      181 aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg
      241 gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc
      301 ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc
      361 agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac
      421 aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat
      481 gccattgctg tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac
      541 cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact gggcgtggag
      601 catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc
      661 tcggcgcgtc tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg
      721 atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg
      781 ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca acgatcagat ggcgctgggc
      841 gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggatatctc ggtagtggga
      901 tacgacgata ccgaagacag ctcatgttat atcccgccgt taaccaccat caaacaggat
      961 tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg
     1021 gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc
     1081 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     1141 gtttcccgac tggaaagcgg gcaggctgca aacgacgaaa actacgcttt agtagcttaa
     1201 taactctgat agtgctagtg tagatctcta ctagagccag gcatcaaata aaacgaaagg
     1261 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
     1321 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagcaatacg
     1381 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     1441 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     1501 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     1561 acaatttcac acatactaga gaaagaggag aaatactaga tggtgagcaa gggcgaggag
     1621 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtgaa cggccacaag
     1681 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     1741 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctgg
     1801 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     1861 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     1921 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     1981 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacatc
     2041 agccacaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag
     2101 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     2161 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     2221 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     2281 gccgggatca ctctcggcat ggacgagctg tacaagaggc ctgctgcaaa cgacgaaaac
     2341 tacgctttag tagcttaata atactagagc caggcatcaa ataaaacgaa aggctcagtc
     2401 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
     2461 cactggctca ccttcgggtg ggcctttctg cgtttata
//