LOCUS       BBa_I3111               1884 bp    DNA     linear       22-Nov-2024
DEFINITION  Test: TetR trigger (I3101.E0422)
ACCESSION   BBa_I3111
VERSION     BBa_I3111.1  
FEATURES             Location/Qualifiers
     promoter        35..40
                     /label=-10
     promoter        15..20
                     /label=-35
     promoter        1..49
                     /label=BBa_R1075
     misc_feature    62..65
                     /label=conserved
     RBS             58..69
                     /label=BBa_B0034
     CDS             79..695
                     /label=tetR
     misc_feature    696..729
                     /label=SsrA
     misc_feature    736..760
                     /label=Help:Barcodes
     CDS             76..735
                     /label=BBa_C0040
     stem_loop       780..823
                     /label=stem_loop
     misc_feature    769..848
                     /label=BBa_B0010
     terminator      769..848
                     /label=BBa_B0010
     stem_loop       864..883
                     /label=T7 TE
     misc_feature    890..890
                     /label=stop
     polyA_site      884..897
                     /label=polya
     misc_feature    857..897
                     /label=BBa_B0012
     terminator      857..897
                     /label=BBa_B0012
     misc_feature    906..924
                     /label=TetR 1
     misc_feature    906..959
                     /label=BBa_R0040
     promoter        925..930
                     /label=-35
     misc_feature    931..949
                     /label=TetR 2
     promoter        948..953
                     /label=-10
     promoter        906..959
                     /label=BBa_R0040
     misc_feature    972..975
                     /label=conserved
     RBS             968..979
                     /label=BBa_B0034
     CDS             986..1747
                     /label=CFP (LVA)
     misc_feature    1704..1741
                     /label=SsrA
     misc_feature    1742..1747
                     /label=2
     misc_feature    1054..1054
                     /label=A
     misc_feature    986..1747
                     /label=BBa_E0022
     misc_feature    986..1747
                     /label=BBa_E0022
     stem_loop       1767..1810
                     /label=stem_loop
     misc_feature    1756..1835
                     /label=BBa_B0010
     terminator      1756..1835
                     /label=BBa_B0010
     stem_loop       1851..1870
                     /label=T7 TE
     misc_feature    1877..1877
                     /label=stop
     polyA_site      1871..1884
                     /label=polya
     misc_feature    1844..1884
                     /label=BBa_B0012
     terminator      1844..1884
                     /label=BBa_B0012
ORIGIN
        1 ttaaatttcc tcttttcagg ccggaataac tccctataat gcgccaccat actagagaaa
       61 gaggagaaat actagatgtc cagattagat aaaagtaaag tgattaacag cgcattagag
      121 ctgcttaatg aggtcggaat cgaaggttta acaacccgta aactcgccca gaagctaggt
      181 gtagagcagc ctacattgta ttggcatgta aaaaataagc gggctttgct cgacgcctta
      241 gccattgaga tgttagatag gcaccatact cacttttgcc ctttagaagg ggaaagctgg
      301 caagattttt tacgtaataa cgctaaaagt tttagatgtg ctttactaag tcatcgcgat
      361 ggagcaaaag tacatttagg tacacggcct acagaaaaac agtatgaaac tctcgaaaat
      421 caattagcct ttttatgcca acaaggtttt tcactagaga atgcattata tgcactcagc
      481 gctgtggggc attttacttt aggttgcgta ttggaagatc aagagcatca agtcgctaaa
      541 gaagaaaggg aaacacctac tactgatagt atgccgccat tattacgaca agctatcgaa
      601 ttatttgatc accaaggtgc agagccagcc ttcttattcg gccttgaatt gatcatatgc
      661 ggattagaaa aacaacttaa atgtgaaagt gggtccgctg caaacgacga aaactacgct
      721 ttagtagctt aataacactg atagtgctag tgtagatcac tactagagcc aggcatcaaa
      781 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
      841 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac
      901 tagagtccct atcagtgata gagattgaca tccctatcag tgatagagat actgagcact
      961 actagagaaa gaggagaaat actagatggt gagcaagggc gaggagctgt tcaccggggt
     1021 ggtgcccatc ctggtcgagc tggacggcga cgtgaacggc cacaagttca gcgtgtccgg
     1081 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     1141 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctggggcg tgcagtgctt
     1201 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     1261 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     1321 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     1381 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacatcagcc acaacgtcta
     1441 tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat
     1501 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     1561 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc
     1621 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     1681 cggcatggac gagctgtaca agaggcctgc tgcaaacgac gaaaactacg ctttagtagc
     1741 ttaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     1801 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
     1861 cgggtgggcc tttctgcgtt tata
//