##gff-version 3
##sequence-region BBa_I3111 1 1884
BBa_I3111	.	promoter	1	49	.	+	0	ID=BBa_R1075;Name=BBa_R1075
BBa_I3111	.	promoter	15	20	.	+	0	ID=annotation309024;Name=-35;Parent=BBa_R1075
BBa_I3111	.	promoter	35	40	.	+	0	ID=annotation309020;Name=-10;Parent=BBa_R1075
BBa_I3111	.	ribosome_entry_site	968	979	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I3111	.	conserved_region	972	975	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I3111	.	terminator	1844	1884	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I3111	.	stem_loop	1851	1870	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I3111	.	engineered_region	1844	1884	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I3111	.	polyA_site	1871	1884	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I3111	.	stop_codon	1877	1877	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I3111	.	promoter	906	959	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I3111	.	promoter	948	953	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I3111	.	non_covalent_binding_site	931	949	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I3111	.	non_covalent_binding_site	906	924	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I3111	.	engineered_region	906	959	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I3111	.	promoter	925	930	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I3111	.	engineered_region	986	1747	.	+	0	ID=BBa_E0022;Name=BBa_E0022
BBa_I3111	.	stop_codon	1742	1747	.	+	0	ID=annotation2154;Name=2;Parent=BBa_E0022
BBa_I3111	.	CDS	986	1747	.	+	0	ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022
BBa_I3111	.	engineered_region	986	1747	.	+	0	ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022
BBa_I3111	.	tag	1704	1741	.	+	0	ID=annotation2153;Name=SsrA;Parent=BBa_E0022
BBa_I3111	.	point_mutation	1054	1054	.	+	0	ID=annotation2155;Name=A;Parent=BBa_E0022
BBa_I3111	.	terminator	769	848	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I3111	.	engineered_region	769	848	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I3111	.	stem_loop	780	823	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I3111	.	CDS	76	735	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_I3111	.	tag	696	729	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_I3111	.	engineered_tag	736	760	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_I3111	.	CDS	79	695	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_I3111	.	terminator	1756	1835	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I3111	.	engineered_region	1756	1835	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I3111	.	stem_loop	1767	1810	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I3111	.	ribosome_entry_site	58	69	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I3111	.	conserved_region	62	65	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I3111	.	terminator	857	897	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I3111	.	stem_loop	864	883	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I3111	.	engineered_region	857	897	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I3111	.	polyA_site	884	897	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I3111	.	stop_codon	890	890	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
>BBa_I3111
ttaaatttcctcttttcaggccggaataactccctataatgcgccaccatactagagaaagaggagaaatactagatgt
ccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccg
taaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgcc
ttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgta
ataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacaga
aaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgca
ctcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaa
cacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagcctt
cttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaa
aactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaa
ggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctca
ccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagag
atactgagcactactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgccc
atcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacg
gcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctg
gggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctac
gtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccc
tggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaacta
catcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatc
gaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgaca
accactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgt
gaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagta
gcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgt
ttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata