LOCUS       BBa_I3112               2234 bp    DNA     linear       22-Nov-2024
DEFINITION  Test: AraC trigger (I3102.E0422)
ACCESSION   BBa_I3112
VERSION     BBa_I3112.1  
FEATURES             Location/Qualifiers
     promoter        35..40
                     /label=-10
     promoter        15..20
                     /label=-35
     promoter        1..49
                     /label=BBa_R1075
     misc_feature    62..65
                     /label=conserved
     RBS             58..69
                     /label=BBa_B0034
     CDS             76..951
                     /label=araC
     misc_feature    952..984
                     /label=LVA
     misc_feature    991..1015
                     /label=Help:Barcodes
     CDS             76..990
                     /label=BBa_C0080
     stem_loop       1035..1078
                     /label=stem_loop
     misc_feature    1024..1103
                     /label=BBa_B0010
     terminator      1024..1103
                     /label=BBa_B0010
     stem_loop       1119..1138
                     /label=T7 TE
     misc_feature    1145..1145
                     /label=stop
     polyA_site      1139..1152
                     /label=polya
     misc_feature    1112..1152
                     /label=BBa_B0012
     terminator      1112..1152
                     /label=BBa_B0012
     misc_feature    1164..1189
                     /label=c-amp2
     misc_feature    1166..1204
                     /label=araO1
     misc_feature    1203..1232
                     /label=c-amp1
     misc_feature    1233..1261
                     /label=ara1 and ara2
     promoter        1273..1278
                     /label=-35
     promoter        1296..1301
                     /label=-10
     promoter        1161..1309
                     /label=BBa_R0080
     misc_feature    1322..1325
                     /label=conserved
     RBS             1318..1329
                     /label=BBa_B0034
     CDS             1336..2097
                     /label=CFP (LVA)
     misc_feature    2054..2091
                     /label=SsrA
     misc_feature    2092..2097
                     /label=2
     misc_feature    1404..1404
                     /label=A
     misc_feature    1336..2097
                     /label=BBa_E0022
     misc_feature    1336..2097
                     /label=BBa_E0022
     stem_loop       2117..2160
                     /label=stem_loop
     misc_feature    2106..2185
                     /label=BBa_B0010
     terminator      2106..2185
                     /label=BBa_B0010
     stem_loop       2201..2220
                     /label=T7 TE
     misc_feature    2227..2227
                     /label=stop
     polyA_site      2221..2234
                     /label=polya
     misc_feature    2194..2234
                     /label=BBa_B0012
     terminator      2194..2234
                     /label=BBa_B0012
ORIGIN
        1 ttaaatttcc tcttttcagg ccggaataac tccctataat gcgccaccat actagagaaa
       61 gaggagaaat actagatggc tgaagcgcaa aatgatcccc tgctgccggg atactcgttt
      121 aacgcccatc tggtggcggg tttaacgccg attgaggcca atggttatct cgattttttt
      181 atcgaccgac cgctgggaat gaaaggttat attctcaatc tcaccattcg cggtcagggg
      241 gtggtgaaaa atcagggacg agaatttgtc tgccgaccgg gtgatatttt gctgttcccg
      301 ccaggagaga ttcatcacta cggtcgtcat ccggaggctc gcgaatggta tcaccagtgg
      361 gtttactttc gtccgcgcgc ctactggcat gaatggctta actggccgtc aatatttgcc
      421 aatacgggtt tctttcgccc ggatgaagcg caccagccgc atttcagcga cctgtttggg
      481 caaatcatta acgccgggca aggggaaggg cgctattcgg agctgctggc gataaatctg
      541 cttgagcaat tgttactgcg gcgcatggaa gcgattaacg agtcgctcca tccaccgatg
      601 gataatcggg tacgcgaggc ttgtcagtac atcagcgatc acctggcaga cagcaatttt
      661 gatatcgcca gcgtcgcaca gcatgtttgc ctgtcgccgt cgcgtctgtc acatcttttc
      721 cgccagcagt tagggattag cgtcttaagc tggcgcgagg accaacgcat cagccaggcg
      781 aagctgcttt tgagcactac ccggatgcct atcgccaccg tcggtcgcaa tgttggtttt
      841 gacgatcaac tctatttctc gcgagtattt aaaaaatgca ccggggccag cccgagcgag
      901 ttccgtgccg gttgtgaaga aaaagtgaat gatgtagccg tcaagttgtc agctgcaaac
      961 gacgaaaact acgctttagt agcttaataa cactgatagt gctagtgtag atcactacta
     1021 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     1081 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
     1141 tctgcgttta tatactagag gcgtaacaaa agtgtctata atcacggcag aaaagtccac
     1201 attgattatt tgcacggcgt cacactttgc tatgccatag catttttatc cataagatta
     1261 gcggatccta cctgacgctt tttatcgcaa ctctctactg tttctccatt actagagaaa
     1321 gaggagaaat actagatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     1381 ctggtcgagc tggacggcga cgtgaacggc cacaagttca gcgtgtccgg cgagggcgag
     1441 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     1501 gtgccctggc ccaccctcgt gaccaccctg acctggggcg tgcagtgctt cagccgctac
     1561 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     1621 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     1681 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     1741 aacatcctgg ggcacaagct ggagtacaac tacatcagcc acaacgtcta tatcaccgcc
     1801 gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat cgaggacggc
     1861 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     1921 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
     1981 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     2041 gagctgtaca agaggcctgc tgcaaacgac gaaaactacg ctttagtagc ttaataatac
     2101 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
     2161 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
     2221 tttctgcgtt tata
//